Bioimage Informatics in the Context of Drosophila Research
Methods xxx (2014) xxx–xxx Contents lists available at ScienceDirect Methods journal homepage: www.elsevier.com/locate/ymeth Bioimage Informatics in the context of Drosophila research ⇑ Florian Jug a,1, Tobias Pietzsch a,1, Stephan Preibisch b,c, Pavel Tomancak a, a Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany b Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA c Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA article info abstract Article history: Modern biological research relies heavily on microscopic imaging. The advanced genetic toolkit of Received 25 February 2014 Drosophila makes it possible to label molecular and cellular components with unprecedented level of Revised 2 April 2014 specificity necessitating the application of the most sophisticated imaging technologies. Imaging in Dro- Accepted 4 April 2014 sophila spans all scales from single molecules to the entire populations of adult organisms, from electron Available online xxxx microscopy to live imaging of developmental processes. As the imaging approaches become more com- plex and ambitious, there is an increasing need for quantitative, computer-mediated image processing Keywords: and analysis to make sense of the imagery. Bioimage Informatics is an emerging research field that covers Image analysis all aspects of biological image analysis from data handling, through processing, to quantitative measure- Registration Processing ments, analysis and data presentation. Some of the most advanced, large scale projects, combining Segmentation cutting edge imaging with complex bioimage informatics pipelines, are realized in the Drosophila Tracking research community. In this review, we discuss the current research in biological image analysis specif- Drosophila ically relevant to the type of systems level image datasets that are uniquely available for the Drosophila model system.
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