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Aplysina Aerophoba Chemical and microbial ecology of the demosponge Aplysina aerophoba Ecología química y microbiana de la demosponja Aplysina aerophoba Oriol Sacristán Soriano ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tdx.cat) i a través del Dipòsit Digital de la UB (diposit.ub.edu) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX ni al Dipòsit Digital de la UB. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX o al Dipòsit Digital de la UB (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR (www.tdx.cat) y a través del Repositorio Digital de la UB (diposit.ub.edu) ha sido autorizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR o al Repositorio Digital de la UB. No se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR o al Repositorio Digital de la UB (framing). Esta reserva de derechos afecta tanto al resumen de presentación de la tesis como a sus contenidos. En la utilización o cita de partes de la tesis es obligado indicar el nombre de la persona autora. WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tdx.cat) service and by the UB Digital Repository (diposit.ub.edu) has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reproduction with lucrative aims is not authorized nor its spreading and availability from a site foreign to the TDX service or to the UB Digital Repository. Introducing its content in a window or frame foreign to the TDX service or to the UB Digital Repository is not authorized (framing). Those rights affect to the presentation summary of the thesis as well as to its contents. In the using or citation of parts of the thesis it’s obliged to indicate the name of the author. Chemical and microbial ecology of the demosponge PhD Thesis 2013 Aplysina aerophoba Oriol Sacristán Soriano aerophoba . A of Chemical and microbial ecology Chemical and microbial O. Sacristán Soriano PhD Thesis 2013 Universidad de Barcelona Facultad de Biología Chemical and microbial ecology of the demosponge Aplysina aerophoba Ecología química y microbiana de la demosponja Aplysina aerophoba Oriol Sacristán Soriano June 2013 Tesis Doctoral Universidad de Barcelona Facultad de Biología Programa de Doctorado de Biodiversidad Chemical and microbial ecology of the demosponge Aplysina aerophoba Memoria presentada por Oriol Sacristán Soriano para optar al Título de Doctor por la Universidad de Barcelona Oriol Sacristán Soriano Centro de Estudios Avanzados de Blanes (CEAB) Consejo Superior de Investigaciones Científicas (CSIC) Barcelona, Junio de 2013 El director de la tesis La tutora de la tesis Dr. Mikel A. Becerro García Dra. Cruz Palacín Cabañas Científico Titular IPNA–CSIC Profesora UB Als meus avis, a mis yayos i al Manel Agradecimientos Como colofón final a esta tesis toca dar las gracias a todas aquellas personas que han hecho posible que este proyecto saliera adelante. Digo “toca” porqué yo no es que me caracterice por el don de la palabra y aunque a algunos ya se lo he agradecido en persona a muchos otros no. Así que me dispongo a escribir unas líneas multilingües para que los destinatarios de esta sección reciban su más que merecido reconocimiento. El primer agradecimiento como es natural va dirigido a Mikel Becerro, mi director en el CEAB y en la distancia en los últimos dos años. Por lo que me has enseñado, por las informales clases de estadística, por tus consejos, y por ver el vaso medio lleno cuando yo lo veía medio vacío. A la Creu Palacín, la tutora d’aquesta tesi, li vull agrair la seva disponibilitat i voluntat eterna per assumir diversos rols. Primer va començar formant part del tribunal del màster experimental que vaig realitzar a la UB, després va assumir la meva tutoria en el màster en Biodiversitat, i finalment ha acabat tutoritzant aquesta tesi doctoral. Je veux remercier beaucoup à Bernard Banaigs pour toute l’aide dans l’analyse chimique des échantillons, sans laquelle la moitié de la thèse n'aurait pas été possible. Gràcies Susanna López per algun que altre consell. Li agraeixo també a l’Emilio O. Casamayor els coneixements que m’ha transmès sobre biologia molecular i el savoir faire en el laboratori. Aunque no he incluido nada de mis estancias en Lisboa y Wageningen en la tesis, quiero agradecer su trabajo a las personas que me acogieron. Muito obrigado Madalena Humanes por me ter recebido na Universidade de Lisboa. Obrigado também a Helena Gaspar por me ter levado a mergulhar. A Alberto Reis pelos viii conselhos no cultivo de bactérias d’esponjas. Estou também muito agradecido a Sandra, a pessoa responsável da minha estadia no ICAT. Thanks to Detmer Sipkema, my supervisor at Wageningen University, for his commitment and guidance during my short stay. Also thanks to all Microbiology lab staff and Detmer’s team for helping me with some lab’s issues. Agradecer también a Juanan Tamayo quien me acogió en su casa y a la comunidad de Spanish por la organización de eventos variopintos. Nunca pensé que podría asistir a tantas barbacoas en tres meses, incluso lloviendo! Agradecimiento a las dos ex integrantes del grupo Charlotte y Villamor, en especial a Adri por sacarme de muestreo y aunque a veces al ofrecerte la mano me cogiste el brazo, una pierna, un trozo de cerebro […] (Villamor, 2010) fue un placer ayudarte y que me ayudaras otras veces. Agrair a la Marta Ribes, a l’Eroteida Jiménez i a la Marta Jové la seva ajuda en l’organització, muntatge i seguiment d’experiments a la ZAE (CMIMA), com també a les persones responsables de la gestió d’aquesta zona d’experimentació, al Maxi i a l’Elvira. Molts són els que m’han donat consells durant les etapes de laboratori. Gemma, JC, Andrea, Xevi, Sònia, Lucía, and specially Patrick who always had an answer that solved all my worries. Altres m’han assistit en la recollecció de mostres com l’Ana Pazó, la Mª João, l’Edu Serrano però, especialment, vull agrair la seva ajuda incondicional al João Gil que mensualment m’acompanyava per estar escassos 20 minuts a l’aigua. Raffaele a ti te agradezco la ayuda que me prestaste en el análisis de imágenes por satélite. Moltes gràcies al CEAB, amb distinció especial per la Carmela i la Gemma que són dos grans pilars pel bon funcionament del centre. També agrair al Ramón el seu suport informàtic. I acabar aquesta part més formal amb l’agraïment al Xavier i a la Iosune, els meus padrins després de la marxa del Mikel al IPNA. He conegut a molta gent durant el meu pas pel CEAB amb els quals he compartit mil històries que han fet que la tesi fos més amena, a vegades tant que oblidaves el motiu pel qual et trobaves ix allà. En una primera fornada ens trobem amb personatges com la Paula risas, Romero, Deivid, Michela, Tina, Mireia, les Lauras Núñez i Serrano, els bessons Oscar i Edu, Ulla, Helena, Ari, Virgis, Mari Carmen, Marc, Valentín, Alis, Hierbas, Jenny, Peckor. Amb ells he compartit birres, congressos, immersions, i amb algun d’ells fins i tot pis. Destacar los consejos de dos doctoras como la copa de un pino, Riesgo y Roci. En una segona fornada podríem destacar al grupo salsero blandense (Steffi, Roser, Edu, Miquel, Laura, Aitana, Dani, Gemma, Susanna, Carmen) amb el qual grans tardes i nits hem compartit. Steffi i Roser gràcies pel vostre suport y por esas magníficas charlas como por esas cervecitas previas para coger fuerzas... como las posteriores para recuperarlas donde se incorporaban Maria Elena, Clara y Lili a veces. Carmeta, mi compañera de piso por excelencia, gracias por el apoyo y por los buenos ratos compartidos, a veces entre algún que otro grito. Willy a ti te agradezco esas tardes de tenis en este final de tesis que hemos compartido. Donatella grazie mille por tus consejos y los buenos momentos que hemos pasado. Dr. Barberán, gracias por las salidas a buscar setas por los alrededores del CEAB, por ese look sexy lady que tanto nos favorece, y por dejarme la tesis para usarla de modelo. Muchas otras personas han participado en este largo camino, en el café, durante las comidas, en eventos varios. Xavi, Pinedo, Guillem, Alessandro, Jordi, Simo, Antoni, Kathrin, Peipoch, Sandra, Juan Pablo, Mariani, Letzy, Alba, Tati, Sara, Marta, Francesco, Chiara, Mirco, Fede, Miguel, Claudia, Carlos, Marias, Celias, Cristina, Tomàs, Boris, Danilo, y alguno más que seguro me dejo (sorry). Fora la vida del CEAB també he trobat persones interessades en el que feia. Durant la universitat vam començar a créixer com a biòlegs encara que al final les sortides per les que ha optat cada un són ben diverses.
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