Characterization of Electrical Penetration Graphs of the Asian Citrus Psyllid, Diaphorina Citri,Insweet Orange Seedlings J
DOI: 10.1111/j.1570-7458.2009.00937.x Characterization of electrical penetration graphs of the Asian citrus psyllid, Diaphorina citri,insweet orange seedlings J. P. Bonani1,A.Fereres2,E.Garzo2,M.P.Miranda3, B. Appezzato-Da-Gloria4 &J.R.S.Lopes1* 1Departamento de Entomologia e Acarologia, ESALQ ⁄ Universidade de Sa˜o Paulo, CP. 9, Piracicaba, SP 13418-900, Brazil, 2Departamento de Proteccio´n Vegetal, Instituto de Ciencias Agrarias (ICA, CSIC), C ⁄ Serrano, 115 dpdo, 28006 Madrid, Spain, 3Fundecitrus, Av. Adhemar de Barros, 201, Araraquara, SP, CEP 14807-040, Brazil, and 4Departamento de Cieˆncias Biolo´gicas, ESALQ ⁄ Universidade de Sa˜o Paulo, CP. 9, Piracicaba, SP 13418-900, Brazil Accepted: 28 September 2009 Key words:Huanglongbing, Citrus sinensis, insect vector, Psyllidae, feeding behavior, EPG, Hemiptera Abstract Detailed information on probing behavior of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is critical for understanding the transmission process of phloem-limited bac- teria (Candidatus Liberibacter spp.) associated with citrus ‘huanglongbing’ by this vector. In this study, we investigated stylet penetration activities of D. citri on seedlings of Citrus sinensis (L.) Osbeck cv. Peˆra (Rutaceae) by using the electrical penetration graph (EPG-DC system) technique. EPG wave- forms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration into plant tissues. The main waveforms were correlated with histological observations of salivary sheath termini in plant tissues, to determine the putative location of stylet tips. The behavioral activities were also inferred based on waveform similarities in relation to other Sternorrhyncha, particularly aphids and whiteflies.
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