DE GRUYTER Physical Sciences Reviews. 2018; 20180008 Marcello Picollo1 / Maurizio Aceto2 / Tatiana Vitorino1,3 UV-Vis spectroscopy 1 Istituto di Fisica Applicata “Nello Carrara” del Consiglio Nazionale delle Ricerche, Via Madonna del piano 10, 50019 Sesto Fiorentino, Firenze, Italy, E-mail:
[email protected],
[email protected] 2 Dipartimento di Scienze e Innovazione Tecnologica (DISIT), Università degli Studi del Piemonte Orientale, viale Teresa Michel, 11, 15121 Alessandria, Italy, E-mail:
[email protected] 3 Department of Conservation and Restoration and LAQV-REQUIMTE, Faculty of Sciences and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal, E-mail:
[email protected] Abstract: UV-Vis reflectance spectroscopy has been widely used as a non-invasive method for the study of cultural her- itage materials for several decades. In particular, FORS, introduced in the 1980s, allows to acquire hundreds of reflectance spectra in situ in a short time, contributing to the identification of artist’s materials. More recently, microspectrofluorimetry has also been proposed as a powerful non-invasive method for the identification of dyes and lake pigments that provides high sensitivity and selectivity. In this chapter, the concepts behind these spectroscopic methodologies will be discussed, as well as the instrumentation and measurement modes used. Case studies related with different cultural heritage materials (paintings and manuscripts, textiles, carpets and tapestries, glass, metals, and minerals),