Verticillium Dahliae Em Solanum Melongena, S

Total Page:16

File Type:pdf, Size:1020Kb

Verticillium Dahliae Em Solanum Melongena, S UNIVERSIDADE DE BRASÍLIA INSTITUTO DE CIÊNCIAS BIOLÓGICAS DEPARTAMENTO DE FITOPATOLOGIA PROGRAMA DE PÓS-GRADUAÇÃO EM FITOPATOLOGIA BUSCA DE FONTES DE RESISTÊNCIA GENÉTICA À VERTICILLIUM DAHLIAE EM SOLANUM MELONGENA, S. STRAMONIFOLIUM E S. SCUTICUM E MANEJO DA IRRIGAÇÃO PARA CONTROLE DA MURCHA DE VERTICÍLIO EM BERINJELA RICARDO NUNES CABRAL Brasília – DF 2015 RICARDO NUNES CABRAL BUSCA DE FONTES DE RESISTÊNCIA GENÉTICA À VERTICILLIUM DAHLIAE NO GÊNERO SOLANUM E MANEJO DA IRRIGAÇÃO PARA O CONTROLE DA MURCHA DE VERTICÍLIO NA BERINJELA. Dissertação apresentada à Universidade de Brasília como requisito parcial para obtenção do título de Mestre em Fitopatologia pelo Programa de Pós-Gradução em Fitopatologia. Orientador Adalberto Corrêa Café Filho, Ph.D. BRASÍLIA DISTRITO FEDERAL - BRASIL 2015 FICHA CATALOGRÁGICA Cabral, Ricardo Nunes Busca de fontes de resistência genética à Verticillium dahliae em Solanum melongena, S. stramonifolium e S. scuticum e manejo da irrigação para controle da murcha de verticílio em berinjela./ Ricardo Nunes Cabral. Brasília, 2015. 92p. Dissertação de mestrado. Programa de Pós-graduação em Fitopatologia. Universidade de Brasília, Brasília. 1. Solanum melongena, Verticillium dahliae, resistência genética vegetal, manejo da irrigação I. Universidade de Brasília. PPG/FIT. II. Busca de fontes de resistência genética à Verticillium dahliae no gênero Solanum e controle cultural baseado na irrigação da murcha de verticílio em berinjela. Dedico esta dissertação a toda minha família, especialmente aos meus pais, Rogéria de Fátima Rangel Nunes e José Carlos de Azevedo Cabral, à minha irmã, Renata Nunes Cabral e à minha namorada Lara Garcia Martos Nunes, pela participação, apoio, incentivo, compreensão, amor e por estarem sempre presentes em minha vida. Amo Vocês ! AGRADECIMENTOS Ao meu professor orientador, Prof. Dr. Adalberto Corrêa Café Filho pela confiança em mim depositada para a realização deste trabalho. Aos Pesquisadores da Embrapa Hortaliças, Dr. Ailton Reis e Dr. Leonardo da Silva Boiteux, pelo envolvimento no projeto e pela ajuda para realizá-lo, disponibilizando sementes do banco de germoplasma e acesso à coleção micológica. Dr. Waldir Aparecido Marouelli, pela colaboração desde a montagem do experimento na Estação Experimental de Biologia da UnB até a finalização da parte escrita. Aos Professores do Departamento de Fitopatologia da UnB, Prof. Dr. Luiz Eduardo Bassay Blum e Prof. Dr. Cleber Furlanetto por aceitarem participar da Banca Examinadora desta dissertação. Ao Professor da Universidade Federal de Viçosa, Prof. Dr. Derly José Henriques da Silva por disponibilizar sementes do Banco de Germoplasma de Hortaliças da UFV e por aceitar participar da Banca Examinadora desta dissertação. A todos os funcionários da Estação Experimental de Biologia da UnB, Aldo, Evandro, Seu Fábio, Seu Chico e Dona Olinda pelo bom humor diário e por toda a ajuda que prestaram no desenvolvimento do experimento. À aluna de graduação em Agronomia - UnB, Julie Anne Lima, pelo interesse na participação neste projeto, facilitando o trabalho realizado no campo. Aos Doutores, Dr. Daniel Anacleto da Costa Lage e Dr. Frederick Mendes Aguiar, por terem ajudado nas análises da dissertação. Aos estudantes do Programa de Pós-Graduação em Fitopatologia da UnB, pelo apoio e pelas amizades durante esses anos. Aos professores e funcionários do Departamento de Fitopatologia – UnB, pelo conhecimento compartilhado. A todos os funcionários da Embrapa Hortaliças. À minha família e amigos por estarem sempre presentes nos momentos importantes da minha vida. Ao CNPq e a FAP/DF pelo financiamento do presente projeto de pesquisa. Muito Obrigado! Ricardo Nunes Cabral. SUMÁRIO RESUMO GERAL ................................................................................................................................. ix GENERAL ABSTRACT ....................................................................................................................... xi INTRODUÇÃO GERAL ........................................................................................................................ 1 REVISÃO DE LITERATURA ............................................................................................................... 3 REFERÊNCIAS BIBLIOGRÁFICAS .................................................................................................. 12 CAPÍTULO 1 ........................................................................................................................................ 17 1. INTRODUÇÃO............................................................................................................................. 22 2. MATERIAIS E MÉTODOS ......................................................................................................... 24 3. RESULTADOS ............................................................................................................................. 29 4. DISCUSSÃO ................................................................................................................................. 34 5. REFERÊNCIAS ............................................................................................................................ 41 CAPÍTULO 2 ........................................................................................................................................ 55 1. INTRODUÇÃO............................................................................................................................. 60 2. MATERIAIS E MÉTODOS ......................................................................................................... 63 3. RESULTADOS ............................................................................................................................. 74 4. DISCUSSÃO ................................................................................................................................. 77 5. CONCLUSÕES FINAIS ............................................................................................................... 84 6. REFERÊNCIAS ............................................................................................................................ 85 v LISTA DE TABELAS Tabela 1.1 – Isolados de Verticillium da coleção micológica do Laboratório de Fitopatologia do CNPH .................................................................................................................................................... 44 Tabela 1.2 – Análise química do solo utilizado nos experimentos....................................................... 44 Tabela 1.3 – Genótipos de Solanum melongena submetidos à avaliação de resistência à Verticillium 45 Tabela 1.4 – Genótipos do gênero Solanum utilizados na avaliação de resistência em Janeiro e Julho de 2014 .................................................................................................................................................. 46 Tabela 1.5 – Genótipos de Solanum scuticum e testemunha utilizados na avaliação de resistência .... 47 Tabela 1.6 - Reação de genótipos de Solanum melongena à mistura de "Vert. 15" e "Vert. 120" ....... 48 Tabela 1.7 – Índice de doença (%) de genótipos de Solanum melongena aos isolados “Vert. 09”; “Vert. 15”; “Vert. 120” e “Vert. 02” ..................................................................................................... 49 Tabela 1.8 – Efeito de diferentes isolados de Verticillium dahliae na altura de genótipos de Solanum melongena ............................................................................................................................................. 49 Tabela 1.9 - Efeito de diferentes isolados de Verticillium dahliae na altura de genótipos de Solanum melongena ............................................................................................................................................. 50 Tabela 1.10 – Dados conjuntos da reação de Solanum stramonifolium à mistura de isolados “Vert. 15” e “Vert. 120”. ........................................................................................................................................ 51 Tabela 1.11 – Reação de genótipos de Solanum scuticum à mistura do isolado “Vert. 120” e “Vert. 15”. ........................................................................................................................................................ 52 Tabela 1.12 – Reação de genótipos de Solanum scuticum à mistura dos isolados “Vert. 120” e “Vert. 15” ......................................................................................................................................................... 53 Tabela 2.1 – Volume de água (L) aplicado por irrigação em cada parcela experimental, conforme a água disponível no solo no momento da irrigação e a profundidade efetiva do sistema radicular da cultura .................................................................................................................................................... 88 Tabela 2.2 – Incidência de murcha de verticílio (%) em plantas de berinjela, conforme a inoculação do solo (Inoc) com Verticillium dahliae e a porcentagem de água disponível no solo (AD) no momento da irrigação ................................................................................................................................................. 88 Tabela 2.3 – Severidade da murcha de verticílio (%) em plantas de berinjela, conforme a inoculação do solo (Inoc) com Verticillium dahliae e a porcentagem
Recommended publications
  • A Higher-Level Phylogenetic Classification of the Fungi
    mycological research 111 (2007) 509–547 available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/mycres A higher-level phylogenetic classification of the Fungi David S. HIBBETTa,*, Manfred BINDERa, Joseph F. BISCHOFFb, Meredith BLACKWELLc, Paul F. CANNONd, Ove E. ERIKSSONe, Sabine HUHNDORFf, Timothy JAMESg, Paul M. KIRKd, Robert LU¨ CKINGf, H. THORSTEN LUMBSCHf, Franc¸ois LUTZONIg, P. Brandon MATHENYa, David J. MCLAUGHLINh, Martha J. POWELLi, Scott REDHEAD j, Conrad L. SCHOCHk, Joseph W. SPATAFORAk, Joost A. STALPERSl, Rytas VILGALYSg, M. Catherine AIMEm, Andre´ APTROOTn, Robert BAUERo, Dominik BEGEROWp, Gerald L. BENNYq, Lisa A. CASTLEBURYm, Pedro W. CROUSl, Yu-Cheng DAIr, Walter GAMSl, David M. GEISERs, Gareth W. GRIFFITHt,Ce´cile GUEIDANg, David L. HAWKSWORTHu, Geir HESTMARKv, Kentaro HOSAKAw, Richard A. HUMBERx, Kevin D. HYDEy, Joseph E. IRONSIDEt, Urmas KO˜ LJALGz, Cletus P. KURTZMANaa, Karl-Henrik LARSSONab, Robert LICHTWARDTac, Joyce LONGCOREad, Jolanta MIA˛ DLIKOWSKAg, Andrew MILLERae, Jean-Marc MONCALVOaf, Sharon MOZLEY-STANDRIDGEag, Franz OBERWINKLERo, Erast PARMASTOah, Vale´rie REEBg, Jack D. ROGERSai, Claude ROUXaj, Leif RYVARDENak, Jose´ Paulo SAMPAIOal, Arthur SCHU¨ ßLERam, Junta SUGIYAMAan, R. Greg THORNao, Leif TIBELLap, Wendy A. UNTEREINERaq, Christopher WALKERar, Zheng WANGa, Alex WEIRas, Michael WEISSo, Merlin M. WHITEat, Katarina WINKAe, Yi-Jian YAOau, Ning ZHANGav aBiology Department, Clark University, Worcester, MA 01610, USA bNational Library of Medicine, National Center for Biotechnology Information,
    [Show full text]
  • REP-PCR, ULTRAESTRUTURA DE LINHAGENS DE Agaricus Bisporus E DE SUA INTERAÇÃO COM Lecanicillium Fungicola
    JANAIRA SANTANA NUNES RIBEIRO REP-PCR, ULTRAESTRUTURA DE LINHAGENS DE Agaricus bisporus E DE SUA INTERAÇÃO COM Lecanicillium fungicola LAVRAS – MG 2014 JANAIRA SANTANA NUNES RIBEIRO REP-PCR, ULTRAESTRUTURA DE LINHAGENS DE Agaricus bisporus E DE SUA INTERAÇÃO COM Lecanicillium fungicola Tese apresentada à Universidade Federal de Lavras, como parte das exigências do Programa de Pós- Graduação em Microbiologia Agrícola, área de concentração em Microbiologia Agrícola, para a obtenção do título de Doutora. Orientador Dr. Eduardo Alves Co-orientador Dr. Eustáquio Souza Dias Dr. Diego Cunha Zied LAVRAS – MG 2014 Ficha catalográfica JANAIRA SANTANA NUNES RIBEIRO REP-PCR, ULTRAESTRUTURA DE LINHAGENS DE Agaricus bisporus E DE SUA INTERAÇÃO COM Lecanicillium fungicola Tese apresentada à Universidade Federal de Lavras, como parte das exigências do Programa de Pós- Graduação em Microbiologia Agrícola, área de concentração em Microbiologia Agrícola, para a obtenção do título de Doutora. APROVADA em 17 de julho de 2014. Dr. Edson Ampélio Pozza UFLA Dr ª Patrícia Gomes Cardoso UFLA Dr ª Simone Cristina Marques UFLA Dr. Diego Cunha Zied UNESP Dr. Eduardo Alves Orientador Dr. Eustáquio Souza Dias Dr. Diego Cunha Zied Co-orientadores LAVRAS – MG 2014 A todos que contribuíram para a realização desse trabalho. DEDICO AGRADECIMENTOS A Deus que me guia e me dá forças para nunca desistir dos meus objetivos. A minha mãe (Maria Santana), irmãos (Francisco Junior, Edna Maria, Rosália Nunes e Jucélia Nunes) e meu marido (Fernando Barreto) ao apoio e incentivo para minha ascensão pessoal e principalmente pela inesgotável confiança nas minhas capacidades. Ao meu orientador Eduardo Alves e co-orientador Eustáquio Sousa Dias, pela confiança, orientação, paciência e ao conhecimento compartilhado durante a realização desse trabalho.
    [Show full text]
  • Mushroom Recipes: Tianguis and Markets of Hidalgo State, Mexico
    Mushroom recipes: tianguis and markets of Hidalgo state, Mexico. Leticia Romero Bautista, Miguel Ángel Islas Santillán, Griselda Pulido Flores y Xanath Valdez Romero Laboratorio de Etnobotánica, Centro de investigaciones Biológicas, Universidad Autónoma del Estado de Hidalgo. Carr. Pachuca-Tulancingo Km 4.5, Mineral de la Reforma, México. C. P. 42184. E mail [email protected] INTRODUCTION Mexican cuisine is known for its great variety of dishes, reflecting the biodiversity of our country, in which organisms interact with cultural expressions and traditions of each geographic region, which gives to each a hallmark. The state of Hidalgo ranks third nationally with more than 260 species of wild edible mushrooms (WEM) and the tradition continues in the markets and swap meets of some municipalities (Fig. 1): Acaxochitlán, Huasca, Huejutla, Mineral del Monte Mineral del Chico, Molango, Omitlán, Pachuca, Zacualtipán and Tlanchinol mainly. MATERIALS AND METHODS Go to these sites selling is an enjoyable experience as they become excellent "information centers" of knowledge, which are provided by “hongueros”, they are people who are responsible for collecting and marketing mushrooms. Species, prices and sales units vary according to the region of the state be purchased heaps, sardine, quadroon, bucket, piece or kilogram and prices range according to the species, the highest are for the most requested and / or difficult to find. Fig. 1 y 2. Sale of mushrooms in a tradicional marketplace Mushrooms species included Phylum Clase Subclase Orden Familia Género Especie Agaricaceae Agaricus bisporus Pleurotus albidus Pleurotaceae Pleurotus djamor 1 Pleurotus ostreatus RESULTS Omphalotaceae Lentinula edodes Agaricales Clitocybe gibba Variety of dishes prepared with 24 WEM and 3 cultivated, acquired in these municipalities are Tricholomataceae Tricholoma caligatum Agaricomycetidae Amanita jacksonii presented in this cookbook.
    [Show full text]
  • Download Full Article in PDF Format
    Cryptogamie, Mycologie, 2016, 37 (4): 449-475 © 2016 Adac. Tous droits réservés Fuscosporellales, anew order of aquatic and terrestrial hypocreomycetidae (Sordariomycetes) Jing YANG a, Sajeewa S. N. MAHARACHCHIKUMBURA b,D.Jayarama BHAT c,d, Kevin D. HYDE a,g*,Eric H. C. MCKENZIE e,E.B.Gareth JONES f, Abdullah M. AL-SADI b &Saisamorn LUMYONG g* a Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand b Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University,P.O.Box 34, Al-Khod 123, Oman c Formerly,Department of Botany,Goa University,Goa, India d No. 128/1-J, Azad Housing Society,Curca, P.O. Goa Velha 403108, India e Manaaki Whenua LandcareResearch, Private Bag 92170, Auckland, New Zealand f Department of Botany and Microbiology,College of Science, King Saud University,P.O.Box 2455, Riyadh 11451, Kingdom of Saudi Arabia g Department of Biology,Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Abstract – Five new dematiaceous hyphomycetes isolated from decaying wood submerged in freshwater in northern Thailand are described. Phylogenetic analyses of combined LSU, SSU and RPB2 sequence data place these hitherto unidentified taxa close to Ascotaiwania and Bactrodesmiastrum. Arobust clade containing anew combination Pseudoascotaiwania persoonii, Bactrodesmiastrum species, Plagiascoma frondosum and three new species, are introduced in the new order Fuscosporellales (Hypocreomycetidae, Sordariomycetes). A sister relationship for Fuscosporellales with Conioscyphales, Pleurotheciales and Savoryellales is strongly supported by sequence data. Taxonomic novelties introduced in Fuscosporellales are four monotypic genera, viz. Fuscosporella, Mucispora, Parafuscosporella and Pseudoascotaiwania.Anew taxon in its asexual morph is proposed in Ascotaiwania based on molecular data and cultural characters.
    [Show full text]
  • Savoryellales (Hypocreomycetidae, Sordariomycetes): a Novel Lineage
    Mycologia, 103(6), 2011, pp. 1351–1371. DOI: 10.3852/11-102 # 2011 by The Mycological Society of America, Lawrence, KS 66044-8897 Savoryellales (Hypocreomycetidae, Sordariomycetes): a novel lineage of aquatic ascomycetes inferred from multiple-gene phylogenies of the genera Ascotaiwania, Ascothailandia, and Savoryella Nattawut Boonyuen1 Canalisporium) formed a new lineage that has Mycology Laboratory (BMYC), Bioresources Technology invaded both marine and freshwater habitats, indi- Unit (BTU), National Center for Genetic Engineering cating that these genera share a common ancestor and Biotechnology (BIOTEC), 113 Thailand Science and are closely related. Because they show no clear Park, Phaholyothin Road, Khlong 1, Khlong Luang, Pathumthani 12120, Thailand, and Department of relationship with any named order we erect a new Plant Pathology, Faculty of Agriculture, Kasetsart order Savoryellales in the subclass Hypocreomyceti- University, 50 Phaholyothin Road, Chatuchak, dae, Sordariomycetes. The genera Savoryella and Bangkok 10900, Thailand Ascothailandia are monophyletic, while the position Charuwan Chuaseeharonnachai of Ascotaiwania is unresolved. All three genera are Satinee Suetrong phylogenetically related and form a distinct clade Veera Sri-indrasutdhi similar to the unclassified group of marine ascomy- Somsak Sivichai cetes comprising the genera Swampomyces, Torpedos- E.B. Gareth Jones pora and Juncigera (TBM clade: Torpedospora/Bertia/ Mycology Laboratory (BMYC), Bioresources Technology Melanospora) in the Hypocreomycetidae incertae
    [Show full text]
  • Hypocreales, Sordariomycetes) from Decaying Palm Leaves in Thailand
    Mycosphere Baipadisphaeria gen. nov., a freshwater ascomycete (Hypocreales, Sordariomycetes) from decaying palm leaves in Thailand Pinruan U1, Rungjindamai N2, Sakayaroj J2, Lumyong S1, Hyde KD3 and Jones EBG2* 1Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand 2BIOTEC Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, NSTDA, 113 Thailand Science Park, Paholyothin Road, Khlong 1, Khlong Luang, Pathum Thani, 12120, Thailand 3School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand Pinruan U, Rungjindamai N, Sakayaroj J, Lumyong S, Hyde KD, Jones EBG 2010 – Baipadisphaeria gen. nov., a freshwater ascomycete (Hypocreales, Sordariomycetes) from decaying palm leaves in Thailand. Mycosphere 1, 53–63. Baipadisphaeria spathulospora gen. et sp. nov., a freshwater ascomycete is characterized by black immersed ascomata, unbranched, septate paraphyses, unitunicate, clavate to ovoid asci, lacking an apical structure, and fusiform to almost cylindrical, straight or curved, hyaline to pale brown, unicellular, and smooth-walled ascospores. No anamorph was observed. The species is described from submerged decaying leaves of the peat swamp palm Licuala longicalycata. Phylogenetic analyses based on combined small and large subunit ribosomal DNA sequences showed that it belongs in Nectriaceae (Hypocreales, Hypocreomycetidae, Ascomycota). Baipadisphaeria spathulospora constitutes a sister taxon with weak support to Leuconectria clusiae in all analyses. Based
    [Show full text]
  • Phylogenetics and Taxonomy of the Fungal Vascular Wilt Pathogen Verticillium, with the Descriptions of Five New Species
    Phylogenetics and Taxonomy of the Fungal Vascular Wilt Pathogen Verticillium, with the Descriptions of Five New Species Patrik Inderbitzin1, Richard M. Bostock1, R. Michael Davis1, Toshiyuki Usami2, Harold W. Platt3, Krishna V. Subbarao1* 1 Department of Plant Pathology, University of California Davis, Davis, California, United States of America, 2 Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan, 3 Agriculture and Agri-Food Canada, Charlottetown Research Centre, Charlottetown, Prince Edward Island, Canada Abstract Knowledge of pathogen biology and genetic diversity is a cornerstone of effective disease management, and accurate identification of the pathogen is a foundation of pathogen biology. Species names provide an ideal framework for storage and retrieval of relevant information, a system that is contingent on a clear understanding of species boundaries and consistent species identification. Verticillium, a genus of ascomycete fungi, contains important plant pathogens whose species boundaries have been ill defined. Using phylogenetic analyses, morphological investigations and comparisons to herbarium material and the literature, we established a taxonomic framework for Verticillium comprising ten species, five of which are new to science. We used a collection of 74 isolates representing much of the diversity of Verticillium, and phylogenetic analyses based on the ribosomal internal transcribed spacer region (ITS), partial sequences of the protein coding genes actin (ACT), elongation factor 1-alpha (EF), glyceraldehyde-3-phosphate dehydrogenase (GPD) and tryptophan synthase (TS). Combined analyses of the ACT, EF, GPD and TS datasets recognized two major groups within Verticillium, Clade Flavexudans and Clade Flavnonexudans, reflecting the respective production and absence of yellow hyphal pigments. Clade Flavexudans comprised V. albo-atrum and V.
    [Show full text]
  • A New Order of Aquatic and Terrestrial Fungi for Achroceratosphaeria and Pisorisporium Gen
    Persoonia 34, 2015: 40–49 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158515X685544 Pisorisporiales, a new order of aquatic and terrestrial fungi for Achroceratosphaeria and Pisorisporium gen. nov. in the Sordariomycetes M. Réblová1, J. Fournier 2, V. Štěpánek3 Key words Abstract Four morphologically similar specimens of an unidentified perithecial ascomycete were collected on decaying wood submerged in fresh water. Phylogenetic analysis of DNA sequences from protein-coding and Achroceratosphaeria ribosomal nuclear loci supports the placement of the unidentified fungus together with Achroceratosphaeria in a freshwater strongly supported monophyletic clade. The four collections are described as two new species of the new genus Hypocreomycetidae Pisorisporium characterised by non-stromatic, black, immersed to superficial perithecial ascomata, persistent para- Koralionastetales physes, unitunicate, persistent asci with an amyloid apical annulus and hyaline, fusiform, cymbiform to cylindrical, Lulworthiales transversely multiseptate ascospores with conspicuous guttules. The asexual morph is unknown and no conidia multigene analysis were formed in vitro or on the natural substratum. The clade containing Achroceratosphaeria and Pisorisporium is systematics introduced as the new order Pisorisporiales, family Pisorisporiaceae in the class Sordariomycetes. It represents a new lineage of aquatic fungi. A sister relationship for Pisorisporiales with the Lulworthiales and Koralionastetales is weakly supported
    [Show full text]
  • An Additional Fungal Lineage in the Hypocreomycetidae (Falcocladium Species) and the Taxonomic Re-Evaluation of Chaetosphaeria C
    Cryptogamie, Mycologie, 2014, 35 (2): 119-138 © 2014 Adac. Tous droits réservés An additional fungal lineage in the Hypocreomycetidae (Falcocladium species) and the taxonomic re-evaluation of Chaetosphaeria chaetosa and Swampomyces species, based on morphology, ecology and phylogeny E.B. Gareth JONESa*, Satinee SUETRONGb, Wan-Hsuan CHENGc, Nattawut RUNGJINDAMAIb, Jariya SAKAYAROJb, Nattawut BOONYUENb, Sayanh SOMROTHIPOLd, Mohamed A. ABDEL-WAHABa & Ka-Lai PANGc aBotany and Microbiology Department, College of Science, King Saud University, Riyadh, 1145, Saudi Arabia; email: [email protected] bFungal Diversity Laboratory (BFBD), National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand cInstitute of Marine Biology and Center of Excellence for the Oceans, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, Taiwan (R.O.C.) dMicrobe Interaction Laboratory (BMIT), National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand Abstract – The taxonomic position of the marine fungi referred to the TBM clade is re-evaluated along with the marine species Chaetosphaeria chaetosa, and the terrestrial asexual genus Falcocladium. Phylogenetic analyses of DNA sequences of two ribosomal nuclear loci of the above taxa and those previous recognized as the TBM clade suggest that they form a distinct clade amongst the Hypocreales, Microascales, Savoryellales, Coronophorales and Melanosporales in the Hypocreomycetidae. Four well-supported subclades in the “TBM clade” are discerned including: 1) the Juncigena subclade, 2) the Etheirophora and Swampomyces s. s. subclade, 3) the Falcocladium subclade and 4) the Torpedospora subclade. Chaetosphaeria chaetosa does not group in the Chaetosphaeriales but together with Swampomyces aegyptiacus and S.
    [Show full text]
  • An Overview of the Systematics of the Sordariomycetes Based on a Four-Gene Phylogeny
    Mycologia, 98(6), 2006, pp. 1076–1087. # 2006 by The Mycological Society of America, Lawrence, KS 66044-8897 An overview of the systematics of the Sordariomycetes based on a four-gene phylogeny Ning Zhang of 16 in the Sordariomycetes was investigated based Department of Plant Pathology, NYSAES, Cornell on four nuclear loci (nSSU and nLSU rDNA, TEF and University, Geneva, New York 14456 RPB2), using three species of the Leotiomycetes as Lisa A. Castlebury outgroups. Three subclasses (i.e. Hypocreomycetidae, Systematic Botany & Mycology Laboratory, USDA-ARS, Sordariomycetidae and Xylariomycetidae) currently Beltsville, Maryland 20705 recognized in the classification are well supported with the placement of the Lulworthiales in either Andrew N. Miller a basal group of the Sordariomycetes or a sister group Center for Biodiversity, Illinois Natural History Survey, of the Hypocreomycetidae. Except for the Micro- Champaign, Illinois 61820 ascales, our results recognize most of the orders as Sabine M. Huhndorf monophyletic groups. Melanospora species form Department of Botany, The Field Museum of Natural a clade outside of the Hypocreales and are recognized History, Chicago, Illinois 60605 as a distinct order in the Hypocreomycetidae. Conrad L. Schoch Glomerellaceae is excluded from the Phyllachorales Department of Botany and Plant Pathology, Oregon and placed in Hypocreomycetidae incertae sedis. In State University, Corvallis, Oregon 97331 the Sordariomycetidae, the Sordariales is a strongly supported clade and occurs within a well supported Keith A. Seifert clade containing the Boliniales and Chaetosphaer- Biodiversity (Mycology and Botany), Agriculture and iales. Aspects of morphology, ecology and evolution Agri-Food Canada, Ottawa, Ontario, K1A 0C6 Canada are discussed. Amy Y.
    [Show full text]
  • Evaluation of PCR-Based Methods for Rapid, Accurate Detection and Monitoring of Verticillium Dahliae in Woody Hosts by Real- Time Polymerase Chain Reaction
    University of Kentucky UKnowledge Theses and Dissertations--Plant Pathology Plant Pathology 2014 Evaluation of PCR-Based Methods for Rapid, Accurate Detection and Monitoring of Verticillium Dahliae in Woody Hosts by Real- Time Polymerase Chain Reaction Baker Diwan Getheeth Aljawasim University of Kentucky, [email protected] Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Aljawasim, Baker Diwan Getheeth, "Evaluation of PCR-Based Methods for Rapid, Accurate Detection and Monitoring of Verticillium Dahliae in Woody Hosts by Real-Time Polymerase Chain Reaction" (2014). Theses and Dissertations--Plant Pathology. 13. https://uknowledge.uky.edu/plantpath_etds/13 This Master's Thesis is brought to you for free and open access by the Plant Pathology at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Plant Pathology by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine) which will be submitted to UKnowledge as Additional File. I hereby grant to The University of Kentucky and its agents the irrevocable, non-exclusive, and royalty-free license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known.
    [Show full text]
  • Sodiomyces Alkalinus, a New Holomorphic Alkaliphilic Ascomycete Within the Plectosphaerellaceae
    Persoonia 31, 2013: 147–158 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158513X673080 Sodiomyces alkalinus, a new holomorphic alkaliphilic ascomycete within the Plectosphaerellaceae A.A. Grum-Grzhimaylo1,3, A.J.M. Debets1, A.D. van Diepeningen2, M.L. Georgieva3, E.N. Bilanenko3 Key words Abstract In this study we reassess the taxonomic reference of the previously described holomorphic alkaliphilic fungus Heleococcum alkalinum isolated from soda soils in Russia, Mongolia and Tanzania. We show that it is not alkaliphilic fungi an actual member of the genus Heleococcum (order Hypocreales) as stated before and should, therefore, be ex- growth cluded from it and renamed. Multi-locus gene phylogeny analyses (based on nuclear ITS, 5.8S rDNA, 28S rDNA, Heleococcum alkalinum 18S rDNA, RPB2 and TEF1-alpha) have displayed this fungus as a new taxon at the genus level within the family molecular phylogeny Plectosphaerellaceae, Hypocreomycetidae, Ascomycota. The reference species of actual Heleococcum members scanning electron microscopy showed clear divergence from the strongly supported Heleococcum alkalinum position within the Plectosphaerel- taxonomy laceae, sister to the family Glomerellaceae. Eighteen strains isolated from soda lakes around the world show remarkable genetic similarity promoting speculations on their possible evolution in harsh alkaline environments. We established the pH growth optimum of this alkaliphilic fungus at c. pH 10 and tested growth on 30 carbon sources at pH 7 and 10. The new genus and species, Sodiomyces alkalinus gen. nov. comb. nov., is the second holomorphic fungus known within the family, the first one being Plectosphaerella – some members of this genus are known to be alkalitolerant.
    [Show full text]