Infertility Investigations for Women
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Evolution of Oviductal Gestation in Amphibians MARVALEE H
THE JOURNAL OF EXPERIMENTAL ZOOLOGY 266394-413 (1993) Evolution of Oviductal Gestation in Amphibians MARVALEE H. WAKE Department of Integrative Biology and Museum of Vertebrate Zoology, University of California,Berkeley, California 94720 ABSTRACT Oviductal retention of developing embryos, with provision for maternal nutrition after yolk is exhausted (viviparity) and maintenance through metamorphosis, has evolved indepen- dently in each of the three living orders of amphibians, the Anura (frogs and toads), the Urodela (salamanders and newts), and the Gymnophiona (caecilians). In anurans and urodeles obligate vivi- parity is very rare (less than 1%of species); a few additional species retain the developing young, but nutrition is yolk-dependent (ovoviviparity) and, at least in salamanders, the young may be born be- fore metamorphosis is complete. However, in caecilians probably the majority of the approximately 170 species are viviparous, and none are ovoviviparous. All of the amphibians that retain their young oviductally practice internal fertilization; the mechanism is cloaca1 apposition in frogs, spermato- phore reception in salamanders, and intromission in caecilians. Internal fertilization is a necessary but not sufficient exaptation (sensu Gould and Vrba: Paleobiology 8:4-15, ’82) for viviparity. The sala- manders and all but one of the frogs that are oviductal developers live at high altitudes and are subject to rigorous climatic variables; hence, it has been suggested that cold might be a “selection pressure” for the evolution of egg retention. However, one frog and all the live-bearing caecilians are tropical low to middle elevation inhabitants, so factors other than cold are implicated in the evolu- tion of live-bearing. -
Luteal Phase Deficiency: What We Now Know
■ OBGMANAGEMENT BY LAWRENCE ENGMAN, MD, and ANTHONY A. LUCIANO, MD Luteal phase deficiency: What we now know Disagreement about the cause, true incidence, and diagnostic criteria of this condition makes evaluation and management difficult. Here, 2 physicians dissect the data and offer an algorithm of assessment and treatment. espite scanty and controversial sup- difficult to definitively diagnose the deficien- porting evidence, evaluation of cy or determine its incidence. Further, while Dpatients with infertility or recurrent reasonable consensus exists that endometrial pregnancy loss for possible luteal phase defi- biopsy is the most reliable diagnostic tool, ciency (LPD) is firmly established in clinical concerns remain about its timing, repetition, practice. In this article, we examine the data and interpretation. and offer our perspective on the role of LPD in assessing and managing couples with A defect of corpus luteum reproductive disorders (FIGURE 1). progesterone output? PD is defined as endometrial histology Many areas of controversy Linconsistent with the chronological date of lthough observational and retrospective the menstrual cycle, based on the woman’s Astudies have reported a higher incidence of LPD in women with infertility and recurrent KEY POINTS 1-4 pregnancy losses than in fertile controls, no ■ Luteal phase deficiency (LPD), defined as prospective study has confirmed these find- endometrial histology inconsistent with the ings. Furthermore, studies have failed to con- chronological date of the menstrual cycle, may be firm the superiority of any particular therapy. caused by deficient progesterone secretion from the corpus luteum or failure of the endometrium Once considered an important cause of to respond appropriately to ovarian steroids. -
Oogenesis [PDF]
Oogenesis Dr Navneet Kumar Professor (Anatomy) K.G.M.U Dr NavneetKumar Professor Anatomy KGMU Lko Oogenesis • Development of ovum (oogenesis) • Maturation of follicle • Fate of ovum and follicle Dr NavneetKumar Professor Anatomy KGMU Lko Dr NavneetKumar Professor Anatomy KGMU Lko Oogenesis • Site – ovary • Duration – 7th week of embryo –primordial germ cells • -3rd month of fetus –oogonium • - two million primary oocyte • -7th month of fetus primary oocyte +primary follicle • - at birth primary oocyte with prophase of • 1st meiotic division • - 40 thousand primary oocyte in adult ovary • - 500 primary oocyte attain maturity • - oogenesis completed after fertilization Dr Navneet Kumar Dr NavneetKumar Professor Professor (Anatomy) Anatomy KGMU Lko K.G.M.U Development of ovum Oogonium(44XX) -In fetal ovary Primary oocyte (44XX) arrest till puberty in prophase of 1st phase meiotic division Secondary oocyte(22X)+Polar body(22X) 1st phase meiotic division completed at ovulation &enter in 2nd phase Ovum(22X)+polarbody(22X) After fertilization Dr NavneetKumar Professor Anatomy KGMU Lko Dr NavneetKumar Professor Anatomy KGMU Lko Dr Navneet Kumar Dr ProfessorNavneetKumar (Anatomy) Professor K.G.M.UAnatomy KGMU Lko Dr NavneetKumar Professor Anatomy KGMU Lko Maturation of follicle Dr NavneetKumar Professor Anatomy KGMU Lko Maturation of follicle Primordial follicle -Follicular cells Primary follicle -Zona pallucida -Granulosa cells Secondary follicle Antrum developed Ovarian /Graafian follicle - Theca interna &externa -Membrana granulosa -Antrial -
Endocrine Control of Lactational Infertility. I
Maternal Nutrition and Lactational Infertility, edited by I. Dobbing. Nestld Nutrition, Vevey/ Raven Press, New York © 1985. Endocrine Control of Lactational Infertility. I *Alan S. McNeilly, *Anna Glasier, and fPeter W. Howie *MRC Reproductive Biology Unit, Edinburgh EH3 9EW, and 1'Department of Obstetrics and Gynaecology, University of Dundee Medical School, Ninewells Hospital, Dundee DD1 951, Scotland Although there is no doubt that breastfeeding suppresses ovarian activity, the reasons for the immense variability in the duration of this suppression and the mechanisms by which the suckling stimulus causes it remain unclear. The interbirth interval in women who breastfeed can be divided into three main components: (a) the period of lactational amenorrhoea, (b) a period when menstruation returns either during or after lactation, and (c) pregnancy. The length of periods a and b will vary considerably depending on the pattern of breastfeeding, and in a few cases pregnancy will occur during the period of lactational amenorrhoea without an intervening period of menstrual cycles. In an attempt to clarify the mechanisms controlling each of periods a and b above, the changes in endocrine and ovarian activities will be explored. GONADOTROPHIC CONTROL OF THE MENSTRUAL CYCLE Before discussing in detail the influences of suckling on ovarian activity, it is first necessary to outline the basic mechanisms controlling the growth and devel- opment of follicles and subsequent formation of the corpus luteum in the normal menstrual cycle. The basic changes in the four principal hormones involved are shown in Fig. 1. At the time of menses following the demise of the corpus luteum of the previous cycle, follicle development starts, and usually a single follicle begins to grow. -
Variability in the Length of Menstrual Cycles Within and Between Women - a Review of the Evidence Key Points
Variability in the Length of Menstrual Cycles Within and Between Women - A Review of the Evidence Key Points • Mean cycle length ranges from 27.3 to 30.1 days between ages 20 and 40 years, follicular phase length is 13-15 days, and luteal phase length is less variable and averages 13-14 days1-3 • Menstrual cycle lengths vary most widely just after menarche and just before menopause primarily as cycles are anovulatory 1 • Mean length of follicular phase declines with age3,11 while luteal phase remains constant to menopause8 • The variability in menstrual cycle length is attributable to follicular phase length1,11 Introduction Follicular and luteal phase lengths Menstrual cycles are the re-occurring physiological – variability of menstrual cycle changes that happen in women of reproductive age. Menstrual cycles are counted from the first day of attributable to follicular phase menstrual flow and last until the day before the next onset of menses. It is generally assumed that the menstrual cycle lasts for 28 days, and this assumption Key Points is typically applied when dating pregnancy. However, there is variability between and within women with regard to the length of the menstrual cycle throughout • Follicular phase length averages 1,11,12 life. A woman who experiences variations of less than 8 13-15 days days between her longest and shortest cycle is considered normal. Irregular cycles are generally • Luteal phase length averages defined as having 8 to 20 days variation in length of 13-14 days1-3 cycle, whereas over 21 days variation in total cycle length is considered very irregular. -
Understanding Your Menstrual Cycle If You're Trying to Conceive
IS MY PERIOD NORMAL? Understanding Your Menstrual Cycle If You’re Trying to Conceive More than 70% 11% 95% of women have or more of of U.S. women start irregular menstrual American women their periods by cycles as menopause suffer from age 16. approaches. endometriosis.1 10% 12% of U.S. women are of women have affected by PCOS trouble getting or (polycystic ovary staying pregnant.3 syndrome).2 Fortunately, your menstrual cycle can tell you a lot about your fertility if you know what to look for. TYPES OF MENSTRUAL CYCLES Only 15% of About Normal = women have 30% of women are fertile only during 21 to 35 days the “perfect” the “normal” fertility 28-day cycle. window—between days 10 and 17 of the menstrual cycle. Day 1 Period starts (aka menses) 27 28 1 2 26 3 25 4 24 5 Day 15-28 23 6 Day 2-14 Luteal phase; Follicular phase; progesterone** 22 WHAT’S NORMAL? 7 FSH released, (follicle- uterine lining 21 8 stimulating matures Give or take a few days, hormone) and a normal cycle looks like this: estrogen released, 20 9 ovulation* begins 19 10 18 11 17 12 16 15 14 13 *ovulation: the process of an ovum (egg) being released from the ovary; occurs 10-14 days before menses. **progesterone: a steroid hormone that tells the uterus to prepare for pregnancy At least 30% of women have an “irregular” cycle either short, long or inconsistent. Short = Long = < 21 days > 35 days May be a sign of: May be a sign of: Hormonal imbalance Hormonal imbalance Ovaries with fewer eggs Lack of ovulation Approach of menopause Other fertility issues Reduced fertility4 Increased risk of miscarriage SIGNS TO WATCH FOR Your menstrual cycle provides valuable clues about your body’s reproductive health. -
A Fixed Formula to Define the Fertile Window of the Menstrual Cycle As the Basis of a Simple Method of Natural Family Planning
ORIGINAL RESEARCH ARTICLE A Fixed Formula to Define the Fertile Window of the Menstrual Cycle as the Basis of a Simple Method of Natural Family Planning Marcos Are´valo,* Irit Sinai,* and Victoria Jennings* A significant number of women worldwide use periodic basis of the proposed Standard Days method, a simple abstinence as their method of family planning. Many of method of natural family planning (NFP). Survey data them use some type of calendar-based approach to deter- from a number of countries around the world show mine when they should abstain from unprotected inter- that a substantial number of women worldwide use course to avoid pregnancy; yet they often lack correct periodic abstinence as their method of family plan- knowledge of when during their menstrual cycle they are ning.1 Many of these women use calendar-based ap- most likely to become pregnant. A simple method of proaches to determine when they should abstain from natural family planning (NFP) based on a fixed formula to unprotected intercourse to avoid pregnancy. How- define the fertile window could be useful to these women. ever, research also indicates that a significant per- This article reports the results of an analysis of the appli- centage of women who claim to use periodic absti- cation of a fixed formula to define the fertile window. A nence lack correct knowledge of when during their large existing data set from a World Health Organization menstrual cycle they are most likely to become study of the Ovulation Method was used to estimate the pregnant.a Most of these women simply abstain from theoretical probability of pregnancy using this formula. -
Changes Before the Change1.06 MB
Changes before the Change Perimenopausal bleeding Although some women may abruptly stop having periods leading up to the menopause, many will notice changes in patterns and irregular bleeding. Whilst this can be a natural phase in your life, it may be important to see your healthcare professional to rule out other health conditions if other worrying symptoms occur. For further information visit www.imsociety.org International Menopause Society, PO Box 751, Cornwall TR2 4WD Tel: +44 01726 884 221 Email: [email protected] Changes before the Change Perimenopausal bleeding What is menopause? Strictly defined, menopause is the last menstrual period. It defines the end of a woman’s reproductive years as her ovaries run out of eggs. Now the cells in the ovary are producing less and less hormones and menstruation eventually stops. What is perimenopause? On average, the perimenopause can last one to four years. It is the period of time preceding and just after the menopause itself. In industrialized countries, the median age of onset of the perimenopause is 47.5 years. However, this is highly variable. It is important to note that menopause itself occurs on average at age 51 and can occur between ages 45 to 55. Actually the time to one’s last menstrual period is defined as the perimenopausal transition. Often the transition can even last longer, five to seven years. What hormonal changes occur during the perimenopause? When a woman cycles, she produces two major hormones, Estrogen and Progesterone. Both of these hormones come from the cells surrounding the eggs. Estrogen is needed for the uterine lining to grow and Progesterone is produced when the egg is released at ovulation. -
Association Between Increased Expression of Endothelial Isoform of Nitric Oxide Synthase in the Human Fallopian Tube and Tubal Ectopic Pregnancy
Iran J Reprod Med Vol. 12. No. 1. pp: 19-28, January 2014 Original article Association between increased expression of endothelial isoform of nitric oxide synthase in the human fallopian tube and tubal ectopic pregnancy Leyla Fath Bayati1 M.Sc., Marefat Ghaffari Novin1, 2 M.D., Ph.D., Fatemeh Fadaei Fathabadi1 Ph.D., Abbas Piryaei1 Ph.D., Mohammad Hasan Heidari1 Ph.D., Mozhgan Bandehpour2 Ph.D., Mohsen Norouzian1 Ph.D., Mahdi Alizadeh Parhizgar3 M.D., Mahmood Shakooriyan Fard3 B.Sc. 1. Department of Biology and Abstract Anatomical Sciences, Faculty of Medicine, Shahid Beheshti Background: Tubal ectopic pregnancy (tEP) is the most common type of extra- University of Medical Sciences, uterine pregnancy and the most common cause of maternal mortality. Nitric oxide Tehran, Iran. (NO) is a molecule that incorporates in many physiological processes of female 2. Cellular and Molecular Biology reproductive system. Recent studies have demonstrated the possible role of Research Center, Shahid Beheshti University of Medical endothelial isoform of nitric oxide synthase (eNOS) enzyme in the regulation of Sciences, Tehran, Iran. many reproductive events that occur in the fallopian tube (FT). 3. Department of Pathology, Objective: The aim of this study was to evaluate the expression of eNOS in the FTs Kamkar Arab-Niya Hospital, of women with tEP. Qom University of Medical Sciences, Qom, Iran. Materials and Methods: In this case-control study, a total number of 30FTs samples were obtained from three groups including: 10 FTs of women that bearing an EP, 10 FTs from the non-pregnant women at luteal phase of the menstrual cycle, and 10 FTs of healthy pregnant women (n=10). -
Implantation of the Human Embryo
14 Implantation of the Human Embryo Russell A. Foulk University of Nevada, School of Medicine USA 1. Introduction Implantation is the final frontier to embryogenesis and successful pregnancy. Over the past three decades, there have been tremendous advances in the understanding of human embryo development. Since the advent of In Vitro Fertilization, the embryo has been readily available to study outside the body. Indeed, the study has led to much advancement in embryonic stem cell derivation. Unfortunately, it is not so easy to evaluate the steps of implantation since the uterus cannot be accessed by most research tools. This has limited our understanding of early implantation. Both the physiological and pathological mechanisms of implantation occur largely unseen. The heterogeneity of these processes between species also limits our ability to develop appropriate animal models to study. In humans, there is a precise coordinated timeline in which pregnancy can occur in the uterus, the so called “window of implantation”. However, in many cases implantation does not occur despite optimal timing and embryo quality. It is very frustrating to both a patient and her clinician to transfer a beautiful embryo into a prepared uterus only to have it fail to implant. This chapter will review the mechanisms of human embryo implantation and discuss some reasons why it fails to occur. 2. Phases of human embryo implantation The human embryo enters the uterine cavity approximately 4 to 5 days post fertilization. After passing down the fallopian tube or an embryo transfer catheter, the embryo is moved within the uterine lumen by rhythmic myometrial contractions until it can physically attach itself to the endometrial epithelium. -
Endocrine and Ovarian Follicular Changes Leading up Ovulation In
Endocrine and ovarian follicular changes leading up to the first ovulation in prepubertal heifers A. C. O. Evans, G. P. Adams and N. C. Rawlings Departments of J Veterinary Physiological Sciences and 2 Veterinary Anatomy, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, S7N OWO, Canada Changes in the pattern of follicular growth and development, and the associated endocrine changes, were examined in prepubertal heifers approaching their first ovulation. Ten, age-matched (\m=+-\3 days), Spring-born Hereford heifers were examined daily by transrectal ultrasonography for 17 days beginning 12 weeks before the first ovulation, and daily from just before the first ovulation until the completion of one normal duration ovulatory cycle. On each day of ultrasound examination, the position and diameter of corpora lutea and follicles \m=ge\3 mm in diameter were recorded, and one blood sample was collected. Blood samples were also collected every 15 min, for 12 h, at 20, 12 and 4 weeks before the first ovulation, to assess the pulsatile nature of LH and FSH secretion. The first ovulation occurred at 56.0 \m=+-\1.2 weeks of age, at a body weight of 391.9 \m=+-\12.0 kg. Waves of follicular development, similar to those of adult cows, were seen at all ages, and in all heifers, the first ovulation was followed by an ovulatory cycle of short duration (7.7 \m=+-\0.2 days) and then by a normal duration ovulatory cycle (20.3 \m=+-\0.5 days). The maximum diameter of the dominant, or largest subordinate, follicles did not increase as the first ovulation approached, or during the subsequent ovulatory cycles. -
Ovulation-Menstruation-Conception”: Teacher’S Guide
6th Puberty Session 2 “Ovulation-Menstruation-Conception”: Teacher’s Guide Chatham County Schools follows the NC Essential Standards. The NC Essential Standards outline the skills and knowledge that students should receive each year in school. The below standards represent the Interpersonal Communication and Relationships standards that students have covered by the completion of 6th grade. The knowledge encompassed by each standard builds yearly, so it is vital that students receive instruction aligning with the standards each year. This is session one of a two part lesson for 6th grade focusing on the Interpersonal Communications and Relationships Healthful Living North Carolina (NC) Essential Standards. This session focuses on 6.ICR.3.2, which focuses on understanding conception and menstruation. It is vital that students understand the concepts of puberty and the reproductive system prior to this lesson. Session One provides this foundation. Statement of Objectives By the end of today’s lesson students will be able to: 6.ICR.3: Understand the changes that occur during puberty and adolescence. o 6.ICR.3.2: Summarize the relationship between conception and the menstrual cycle. Time: 90 minutes Materials: Projector Slides (“6th Puberty Presentation_Day 2”) Teacher copy: "6th Grade Puberty Frequently Asked Questions” (separate document) Teacher copy: “6th Grade Puberty Difficult Questions” (teacher copy) (separate document) “Background Content for Facilitating Bowl and Spoon Activity” (pg 6) Items for Bowl and Spoon Activity” (pg