16 Antibody [CDH16/1071] Cat. No.: 33-309

Cadherin 16 Antibody [CDH16/1071]

Formalin-fixed, paraffin-embedded rat kidney stained with Cadherin 16 antibody (CDH16/1071)

Specifications

HOST SPECIES: Mouse

SPECIES REACTIVITY: Dog, Human, Mouse, Rabbit, Rat

IMMUNOGEN: Recombinant human protein was used as the immunogen for the Cadherin 16 antibody.

TESTED APPLICATIONS: Flow, IF, IHC-P

September 26, 2021 1 https://www.prosci-inc.com/cadherin-16-antibody-cdh16-1071-33-309.html Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2) APPLICATIONS:

Optimal dilution of the Cadherin 16 antibody to be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

PURIFICATION: Protein G affinity chromatography

CLONALITY: Monoclonal

ISOTYPE: IgG1, kappa

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

CONCENTRATION: 0.2 mg/mL

STORAGE CONDITIONS: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Additional Info

OFFICIAL SYMBOL: CDH16

ALTERNATE NAMES: Cadherin-16, Kidney-specific cadherin, Ksp-cadherin, CDH16

GENE ID: 1014

USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background and References

This mAb recognizes a protein of 130kDa, identified as Ksp-cadherin. form a superfamily of related that mediate calcium-dependent and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue , tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, BACKGROUND: and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

September 26, 2021 2 https://www.prosci-inc.com/cadherin-16-antibody-cdh16-1071-33-309.html ANTIBODIES FOR RESEARCH USE ONLY.

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