Cadherin 16 Antibody [CDH16/1071] Cat. No.: 33-309 Cadherin 16 Antibody [CDH16/1071] Formalin-fixed, paraffin-embedded rat kidney stained with Cadherin 16 antibody (CDH16/1071) Specifications HOST SPECIES: Mouse SPECIES REACTIVITY: Dog, Human, Mouse, Rabbit, Rat IMMUNOGEN: Recombinant human protein was used as the immunogen for the Cadherin 16 antibody. TESTED APPLICATIONS: Flow, IF, IHC-P September 26, 2021 1 https://www.prosci-inc.com/cadherin-16-antibody-cdh16-1071-33-309.html Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) APPLICATIONS: Optimal dilution of the Cadherin 16 antibody to be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Properties PURIFICATION: Protein G affinity chromatography CLONALITY: Monoclonal ISOTYPE: IgG1, kappa CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide CONCENTRATION: 0.2 mg/mL STORAGE CONDITIONS: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. Additional Info OFFICIAL SYMBOL: CDH16 ALTERNATE NAMES: Cadherin-16, Kidney-specific cadherin, Ksp-cadherin, CDH16 GENE ID: 1014 USER NOTE: Optimal dilutions for each application to be determined by the researcher Background and References This mAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, BACKGROUND: and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma. September 26, 2021 2 https://www.prosci-inc.com/cadherin-16-antibody-cdh16-1071-33-309.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/cadherin-16-antibody-cdh16-1071-33-309.html.