Across the Tree of Life, Radiation Resistance Is Governed By
Across the tree of life, radiation resistance is PNAS PLUS + governed by antioxidant Mn2 , gauged by paramagnetic resonance Ajay Sharmaa,1, Elena K. Gaidamakovab,c,1, Olga Grichenkob,c, Vera Y. Matrosovab,c, Veronika Hoekea, Polina Klimenkovab,c, Isabel H. Conzeb,d, Robert P. Volpeb,c, Rok Tkavcb,c, Cene Gostincarˇ e, Nina Gunde-Cimermane, Jocelyne DiRuggierof, Igor Shuryakg, Andrew Ozarowskih, Brian M. Hoffmana,i,2, and Michael J. Dalyb,2 aDepartment of Chemistry, Northwestern University, Evanston, IL 60208; bDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814; cHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817; dDepartment of Biology, University of Bielefeld, Bielefeld, 33615, Germany; eDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, SI-1000, Slovenia; fDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218; gCenter for Radiological Research, Columbia University, New York, NY 10032; hNational High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32306; and iDepartment of Molecular Biosciences, Northwestern University, Evanston, IL 60208 Contributed by Brian M. Hoffman, September 15, 2017 (sent for review August 1, 2017; reviewed by Valeria Cizewski Culotta and Stefan Stoll) Despite concerted functional genomic efforts to understand the agents of cellular damage. In particular, irradiated cells rapidly form •− complex phenotype of ionizing radiation (IR) resistance, a genome superoxide (O2 ) ions by radiolytic reduction of both atmospheric sequence cannot predict whether a cell is IR-resistant or not. Instead, O2 and O2 released through the intracellular decomposition of IR- we report that absorption-display electron paramagnetic resonance generated H2O2 ascatalyzedbybothenzymaticandnonenzymatic (EPR) spectroscopy of nonirradiated cells is highly diagnostic of IR •− metal ions.
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