Notes on Larval Growth and Efficiencies in Antheraea: A
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Bombyx Mori Silk Fibroin Scaffolds with Antheraea Pernyi Silk Fibroin Micro/Nano Fibers for Promoting EA
Article Bombyx mori Silk Fibroin Scaffolds with Antheraea pernyi Silk Fibroin Micro/Nano Fibers for Promoting EA. hy926 Cell Proliferation Yongchun Chen, Weichao Yang, Weiwei Wang, Min Zhang and Mingzhong Li * National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, Jiangsu, China; [email protected] (Y.C.); [email protected] (W.Y.); [email protected] (W.W.); [email protected] (M.Z.) * Correspondence: [email protected]; Tel.: +86‐512‐6706‐1150 Received: 24 August 2017; Accepted: 30 September 2017; Published: 3 October 2017 Abstract: Achieving a high number of inter‐pore channels and a nanofibrous structure similar to that of the extracellular matrix remains a challenge in the preparation of Bombyx mori silk fibroin (BSF) scaffolds for tissue engineering. In this study, Antheraea pernyi silk fibroin (ASF) micro/nano fibers with an average diameter of 324 nm were fabricated by electrospinning from an 8 wt % ASF solution in hexafluoroisopropanol. The electrospun fibers were cut into short fibers (~0.5 mm) and then dispersed in BSF solution. Next, BSF scaffolds with ASF micro/nano fibers were prepared by lyophilization. Scanning electron microscope images clearly showed connected channels between macropores after the addition of ASF micro/nano fibers; meanwhile, micro/nano fibers and micropores could be clearly observed on the pore walls. The results of in vitro cultures of human umbilical vein endothelial cells (EA. hy926) on BSF scaffolds showed that fibrous BSF scaffolds containing 150% ASF fibers significantly promoted cell proliferation during the initial stage. -
Purification of a Lectin from the Hemolymph of Chinese Oak Silk Moth (Antheraea Pernyi) Pupae1
J. Biochem. 101, 545-551 (1987) Purification of a Lectin from the Hemolymph of Chinese Oak Silk Moth (Antheraea pernyi) Pupae1 Xian-Ming QU,2 Chun-Fa ZHANG,3 Hiroto KOMANO, and Shunji NATORI Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113 Received for publication, November 7, 1986 A lectin with affinity to galactose was purified to homogeneity from the hemolymph of diapausing pupae of the Chinese oak silk moth, Antheraea pernyi. The molecular mass of this lectin was 380,000 and it formed an oligomeric structure of a subunit with a molecular mass of 38,000. The hemagglutinating activity in the hemolymph was found to increase with time after immunization with E. coli. Studies with antibody against the purified lectin showed that increase in the hemagglutinating activity was due to the same lectin, suggesting that the amount of the lectin increased in response to intrusion of foreign substances. The function of this lectin in the defence mechanism is discussed. The hemolymphs of many invertebrates are known have no definite humoral immune system like that to contain agglutinin (1-7). Most of these agglu in vertebrates, one of the biological roles of inver tinins are proteins binding carbohydrates, so they tebrate lectins has been suggested to be in the can be defined as invertebrate lectins. These lec defence mechanism (8-11). However, no conclu tins differ greatly in molecular masses, subunit sive evidence to support this idea has yet been structures, hapten sugars, and ionic requirements obtained. (7). Probably, these lectins participate in many Previously, we purified a lectin from the aspects of fundamental biological events, such as hemolymph of Sarcophaga peregrina (flesh-fly) development, differentiation, recognition of self larvae (12). -
PORTABLE OUTDOOR CAGES for MATING FEMALE GIANT SILKWORM MOTHS (SATURNIIDAE)L
VOLUME 30, NUMBER 2 95 PORTABLE OUTDOOR CAGES FOR MATING FEMALE GIANT SILKWORM MOTHS (SATURNIIDAE)l THOMAS A. MILLER 2 AND WILLIAM J. COOPER ~ U. S. Army Medical Bioengineering Research and Development Laboratory, USAMBRDL BLDG 568, Fort Detrick, Maryland 21701 Like many lepidopterists who colonize and study the saturniids, we must find time apart from professional duties and other daily activities to pursue our interest in this family of moths. Therefore, our rearing methods must be both efficient and reliable if we are to accomplish any thing other than the mere maintenance of colonies. Although most of the saturniid species we have reared can be colonized for many genera tions without difficulty, we have at times had a particular problem obtain ing fertile eggs to perpetuate existing colonies or to begin new ones. This problem has been a function largely of limited available time to ensure the mating of females either by hand mating or by the use of indoor cages. For this reason we undertook an evaluation of methods for the routine, unattended mating of female saturniids indigenous to our area. We decided beforehand that the tying out of virgin females and the use of various-size stationary cages or traps (Collins & Weast, 1961; Quesada, 1967; Villiard, 1969) were not suitable to meet our requirements. Our approach, therefore, was to construct and test a series of portable out door cages that could be substituted locally for tying out or stationary cages and could also be easily transported for use in remote field areas. These cages were designed to minimize or prevent the escape of females placed therein while permitting pheromone-seeking males access for the purpose of copulation. -
Clicking Caterpillars: Acoustic Aposematism in Antheraea Polyphemus and Other Bombycoidea Sarah G
993 The Journal of Experimental Biology 210, 993-1005 Published by The Company of Biologists 2007 doi:10.1242/jeb.001990 Clicking caterpillars: acoustic aposematism in Antheraea polyphemus and other Bombycoidea Sarah G. Brown1, George H. Boettner2 and Jayne E. Yack1,* 1Department of Biology, Carleton University, Ottawa, Ontario, K1S 5B6, Canada and 2Plant Soil and Insect Sciences, University of Massachusetts, Amherst, MA 01003, USA *Author for correspondence (e-mail: [email protected]) Accepted 21 November 2006 Summary Acoustic signals produced by caterpillars have been correlated sound production with attack, and an increase documented for over 100 years, but in the majority of in attack rate was positively correlated with the number of cases their significance is unknown. This study is the first signals produced. In addition, sound production typically to experimentally examine the phenomenon of audible preceded or accompanied defensive regurgitation. sound production in larval Lepidoptera, focusing on a Bioassays with invertebrates (ants) and vertebrates (mice) common silkmoth caterpillar, Antheraea polyphemus revealed that the regurgitant is deterrent to would-be (Saturniidae). Larvae produce airborne sounds, predators. Comparative evidence revealed that other resembling ‘clicks’, with their mandibles. Larvae typically Bombycoidea species, including Actias luna (Saturniidae) signal multiple times in quick succession, producing trains and Manduca sexta (Sphingidae), also produce airborne that last over 1·min and include 50–55 clicks. Individual sounds upon attack, and that these sounds precede clicks within a train are on average 24.7·ms in duration, regurgitation. The prevalence and adaptive significance of often consisting of multiple components. Clicks are audible warning sounds in caterpillars is discussed. -
Insects That Feed on Trees and Shrubs
INSECTS THAT FEED ON COLORADO TREES AND SHRUBS1 Whitney Cranshaw David Leatherman Boris Kondratieff Bulletin 506A TABLE OF CONTENTS DEFOLIATORS .................................................... 8 Leaf Feeding Caterpillars .............................................. 8 Cecropia Moth ................................................ 8 Polyphemus Moth ............................................. 9 Nevada Buck Moth ............................................. 9 Pandora Moth ............................................... 10 Io Moth .................................................... 10 Fall Webworm ............................................... 11 Tiger Moth ................................................. 12 American Dagger Moth ......................................... 13 Redhumped Caterpillar ......................................... 13 Achemon Sphinx ............................................. 14 Table 1. Common sphinx moths of Colorado .......................... 14 Douglas-fir Tussock Moth ....................................... 15 1. Whitney Cranshaw, Colorado State University Cooperative Extension etnomologist and associate professor, entomology; David Leatherman, entomologist, Colorado State Forest Service; Boris Kondratieff, associate professor, entomology. 8/93. ©Colorado State University Cooperative Extension. 1994. For more information, contact your county Cooperative Extension office. Issued in furtherance of Cooperative Extension work, Acts of May 8 and June 30, 1914, in cooperation with the U.S. Department of Agriculture, -
Evidence for Paternal Leakage in Hybrid Periodical Cicadas (Hemiptera: Magicicada Spp.) Kathryn M
Evidence for Paternal Leakage in Hybrid Periodical Cicadas (Hemiptera: Magicicada spp.) Kathryn M. Fontaine¤, John R. Cooley*, Chris Simon Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, Connecticut, United States of America Mitochondrial inheritance is generally assumed to be maternal. However, there is increasing evidence of exceptions to this rule, especially in hybrid crosses. In these cases, mitochondria are also inherited paternally, so ‘‘paternal leakage’’ of mitochondria occurs. It is important to understand these exceptions better, since they potentially complicate or invalidate studies that make use of mitochondrial markers. We surveyed F1 offspring of experimental hybrid crosses of the 17-year periodical cicadas Magicicada septendecim, M. septendecula, and M. cassini for the presence of paternal mitochondrial markers at various times during development (1-day eggs; 3-, 6-, 9-week eggs; 16-month old 1st and 2nd instar nymphs). We found evidence of paternal leakage in both reciprocal hybrid crosses in all of these samples. The relative difficulty of detecting paternal mtDNA in the youngest eggs and ease of detecting leakage in older eggs and in nymphs suggests that paternal mitochondria proliferate as the eggs develop. Our data support recent theoretical predictions that paternal leakage may be more common than previously estimated. Citation: Fontaine KM, Cooley JR, Simon C (2007) Evidence for Paternal Leakage in Hybrid Periodical Cicadas (Hemiptera: Magicicada spp.). PLoS ONE 2(9): e892. doi:10.1371/journal.pone.0000892 INTRODUCTION The seven currently-recognized 13- and 17-year periodical Although mitochondrial DNA (mtDNA) exhibits a variety of cicada species (Magicicada septendecim {17}, M. tredecim {13}, M. -
Preliminary Checklist of the Names of the Worldwide Genus Antheraea
ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Galathea, Berichte des Kreises Nürnberger Entomologen e.V. Jahr/Year: 2000 Band/Volume: 9_Supp Autor(en)/Author(s): Paukstadt Ulrich, Brosch Ulrich, Paukstadt Laela Hayati Artikel/Article: Preliminary Checklist of the Names of the Worldwide Genus Antheraea Hübner, 1819 ("1816") (Lepidoptera: Saturniidae) 1-59 ©Kreis Nürnberger Entomologen; download unter www.biologiezentrum.at Preliminary Checklist of the Names of the Worldwide Genus Antheraea H übner , 1819 (“1816”) (Lepidoptera: Saturniidae) Part I Ulrich Paukstadt, Ulrich Brosch & Laela H ayati Paukstadt galathea - Berichte des Kreises Nürnberger Entomologen e. V Supplement 9 Nürnberg August 2000 1 Contents Zusammenfassung.....................................................................................................3©Kreis Nürnberger Entomologen; download unter www.biologiezentrum.at Key W ords................................................................................................................. 4 Introduction................................................................................................................5 C hapter I Checklist of names above generic-group names...........................................................7 Checklist of generic-group names................................................................................ 7 First Subgenus Antheraea Hübner, 1819 (“ 1816”) 7 Second Subgenus Antheraeopsis -
Characterization of Bombyx Mori and Antheraea Pernyi Silk Fibroins And
Prog Biomater DOI 10.1007/s40204-016-0057-3 ORIGINAL RESEARCH Characterization of Bombyx mori and Antheraea pernyi silk fibroins and their blends as potential biomaterials 1 1,2,3,4,5 4 Shuko Suzuki • Traian V. Chirila • Grant A. Edwards Received: 8 August 2016 / Accepted: 17 October 2016 Ó The Author(s) 2016. This article is published with open access at Springerlink.com Abstract Fibroin proteins isolated from the cocoons of appeared to be adequate for surgical manipulation, as the certain silk-producing insects have been widely investi- modulus and strength surpassed those of BM silk fibroin gated as biomaterials for tissue engineering applications. In alone. It was noticed that high concentrations of AP silk this study, fibroins were isolated from cocoons of domes- fibroin led to a significant reduction in the elasticity of ticated Bombyx mori (BM) and wild Antheraea pernyi (AP) membranes. silkworms following a degumming process. The object of this study was to obtain an assessment on certain properties Keywords Bombyx mori silk Á Antheraea pernyi silk Á Silk of these fibroins in order that a concept might be had fibroins Á Membranes Á Mechanical properties Á FTIR regarding the feasibility of using their blends as biomate- analysis rials. Membranes, 10–20 lm thick, which are water-in- soluble, flexible and transparent, were prepared from pure Abbreviations fibroins and from their blends, and subjected to water vapor BMSF Bombyx mori silk fibroin annealing in vacuum, with the aim of providing materials APSF Antheraea pernyi silk fibroin sufficiently strong for manipulation. The resulting materi- FTIR-ATR Fourier transform infrared-attenuated total als were characterized by electrophoretic analysis and reflectance infrared spectrometry. -
Hour-Glass Behavior of the Circadian Clock Controlling Eclosion of the Silkmoth Antheraea Pernyi
Proceedings of the National Academy of Sciences Vol. 68, No. 3, pp. 595-599, March 1971 Hour-Glass Behavior of the Circadian Clock Controlling Eclosion of the Silkmoth Antheraea pernyi JAMES W. TRUMAN The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138 Communicated by Carroll M. Williams, December 18, 1970 ABSTRACT The emergence of the Pernyi silkmoth thereby established (3). The phase assumed by the new from the pupal exuviae is dictated by a brain-centered, rhythm proves to be a function of the time at which the flash photosensitive clock. In continuous darkness the clock displays a persistent free-running rhythm. In photoperiod is administered during the free-running cycle. The experimen- regimens the interaction of the clock with the daily light- tally determined function permits one to plot a "phase-re- dark cycle produces a characteristic time of eclosion. sponse curve." For our present purposes, the phase-response But, in the majority of regimens (from 23L:ID to 4L:20D), relationship is of interest because it has been used success- the eclosion clock undergoes a discontinuous "hour- fully by Pittendrigh to document the operation .of the "eclo- glass" behavior. Thus, during each daily cycle, the onset of darkness initiates a free-running cycle of the clock. sion clock" throughout the days preceding eclosion (5). The next "lights-on" interrupts this cycle and the clock In the case of Drosophila, exposure to continuous light comes to a stop late in the photophase. The moment causes a dissolution of the eclosion rhythm over the course of when the Pernyi clock stops signals the release of an a few cycles. -
Oak Silkworm, Antheraea Pernyi-Specifically, Its Separation By
1572 BIOCHEMISTRY: BARTH, BUNYARD, AND HAMILTON PROC. N. A. S. and F. Bendall, Nature, 186, 136 (1960); see articles by L. N. M. Duysens, E. Rabinowitch, H. T. Witt, D. I. Arnon, and others, in Photosynthetic Mechanisms of Green Plants, NAS-NRC Pub. 1145 (1963). 2 Franck, J., and J. L. Rosenberg, in Photosynthetic Mechanisms of Green Plants, NAS-NRC Pub. 1145 (1963), p. 101. I Kok, B., in Photosynthetic Mechanisms of Green Plants, p. 45. 4Arnold, W., "An electron-hole picture of photosynthesis," unpublished manuscript. I Kok, B., Plant Physiol., 34, 185 (1959). 6 Govindjee, and J. Spencer, paper presented at the 8th Annual Biophysics Meeting, Chicago, 1964, unpublished manuscript. 7Franck, J., in Photosynthesis in Plants (Ames, Iowa: Iowa State College, 1949), chap. XVI, p. 293. 8Brugger, J. E., in Research in Photosynthesis (New York: Interscience, 1957), p. 113. 9 Duysens, L. N. M., and H. E. Sweers, in Studies on Microalgae and Photosynthetic Bacteria (Tokyo: University of Tokyo Press, 1963), p. 353. 10 Butler, W. L., Plant Physiol., Suppl. 36, IV (1961). 11 Teale, F. J. W., Biochem. J., 85, 148 (1962). 12 Rosenberg, J. L., and T. Bigat, in Photosynthetic Mechanisms of Green Plants, NAS-NRC Pub. 1145 (1963), p. 122. 13 Govindjee, in Photosynthetic Mechanisms of Green Plants, p. 318. 14 Govindjee, and L. Yang, paper presented at the Xth International Botanical Congress, Edinburgh, Scotland, 1964, unpublished manuscript. 11 Butler, W. L., in Photosynthetic Mechanisms of Green Plants, NAS-NRC Pub. 1145 (1963), p. 91. 16 Bannister, T. T., and M. J. Vrooman, Plant Physiol., 39, 622 (1964). -
In Response to Sex-Pheromone Loss in the Large Silk Moth
I exp Biol 137, 29-38 (1988) Printed in Great Britain 0 The Company of Biologists Limited 1988 MEASURED BEHAVIOURAL LATENCY! IN RESPONSE TO SEX-PHEROMONE LOSS IN THE LARGE SILK MOTH *Ç ANTHERAEA POLYPHEMUS BYT C. BAKER ; Division of Toxicology and Physiology, Department of Entomology, University of California, Riverside, CA 92521, USA AND R G VOGT* Institute for Neuroscience, University of Oregon, Eugene, OR 97403, USA Accepted 15 February 1988 Summary Males of the giant silk moth Antheraea polyphemus Cramer (Lepidoptera: Saturniidae) were video-recorded in a sustained-flight wind tunnel in a constant plume of sex pheromone The plume was experimentally truncated, and the moths, on losing pheromone stimulus, rapidly changed their behaviour from up- tunnel zig-zag flight to lateral casting flight The latency of this change was in the range 300-500 ms Video and computer analysis of flight tracks indicates that these moths effect this switch by increasing their course angle to the wind while decreasing their air speed Combined with previous physiological and biochemical data concerning pheromone processing within this species, this behavioural study supports the argument that the temporal limit for this behavioural response latency is determined at the level of genetically coded kinetic processes located within the peripheral sensory hairs Introduction The males of numerous moth species have been shown to utilize two distinct behaviour patterns during sex-pheromone-mediated flight In the presence of pheromone they zig-zag upwind, making -
Polyphemus Moth Antheraea Polyphemus (Cramer) (Insecta: Lepidoptera: Saturniidae: Saturniinae)1 Donald W
EENY-531 Polyphemus Moth Antheraea polyphemus (Cramer) (Insecta: Lepidoptera: Saturniidae: Saturniinae)1 Donald W. Hall2 Introduction Distribution The polyphemus moth, Antheraea polyphemus (Cramer), Polyphemus moths are our most widely distributed large is one of our largest and most beautiful silk moths. It is silk moths. They are found from southern Canada down named after Polyphemus, the giant cyclops from Greek into Mexico and in all of the lower 48 states, except for mythology who had a single large, round eye in the middle Arizona and Nevada (Tuskes et al. 1996). of his forehead (Himmelman 2002). The name is because of the large eyespots in the middle of the moth is hind wings. Description The polyphemus moth also has been known by the genus name Telea, but it and the Old World species in the genus Adults Antheraea are not considered to be sufficiently different to The adult wingspan is 10 to 15 cm (approximately 4 to warrant different generic names. Because the name Anther- 6 inches) (Covell 2005). The upper surface of the wings aea has been used more often in the literature, Ferguson is various shades of reddish brown, gray, light brown, or (1972) recommended using that name rather than Telea yellow-brown with transparent eyespots. There is consider- to avoid confusion. Both genus names were published in able variation in color of the wings even in specimens from the same year. For a historical account of the polyphemus the same locality (Holland 1968). The large hind wing moth’s taxonomy see Ferguson (1972) or Tuskes et al. eyespots are ringed with prominent yellow, white (partial), (1996).