Anti-MURF2 / TRIM55 Antibody (ARG63312)

Product datasheet [email protected]

ARG63312 Package: 100 μg anti-MURF2 / TRIM55 antibody Store at: -20°C

Summary

Product Description Goat Polyclonal antibody recognizes MURF2 / TRIM55

Tested Reactivity Hu, Ms

Predict Reactivity Dog, Rat, Pig

Tested Application WB

Specificity This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).

Host Goat

Clonality Polyclonal

Isotype IgG

Target Name MURF2 / TRIM55

Antigen Species Human

Immunogen C-HIFSFSWLNSLNE

Conjugation Un-conjugated

Alternate Names Muscle-specific RING finger protein 2; muRF2; MuRF-2; MURF-2; RNF29; MuRF2; RING finger protein 29; Tripartite motif-containing protein 55

Application Instructions

Application Note WB: Recommend incubate at RT for 1h. Approx 85 kDa band observed in Human and Mouse Heart lysates (calculated MW of 60.5 kDa according to NP_908973.1). The observed molecular weight corresponds to earlier findings in literature with the same antibody (Willis et al, Circ Res. 2007 Mar 2;100(4):456-9. PMID: 17272810). Recommended concentration: 0.1-0.3 µg/ml.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid

Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA

Preservative 0.02% Sodium azide

Stabilizer 0.5% BSA

Concentration 0.5 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated www.arigobio.com 1/2 freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links GeneID: 84675 Human

Swiss-port # Q9BYV6 Human

Background The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein- protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Resrarch Area Cell Biology and Cellular Response antibody; Gene Regulation antibody

Images

ARG63312 anti-MURF2 / TRIM55 antibody WB image

Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG63312 anti-MURF2 / TRIM55 antibody at 0.1 µg/ml dilution.

ARG63312 anti-MURF2 / TRIM55 antibody WB image

Western Blot: Mouse heart lysate from MURF2 +/+ or MURF2 -/- mouse stained with ARG63312 anti-MURF2 / TRIM55 antibody at 1:500 dilution.