Product datasheet [email protected] ARG63312 Package: 100 μg anti-MURF2 / TRIM55 antibody Store at: -20°C Summary Product Description Goat Polyclonal antibody recognizes MURF2 / TRIM55 Tested Reactivity Hu, Ms Predict Reactivity Dog, Rat, Pig Tested Application WB Specificity This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.). Host Goat Clonality Polyclonal Isotype IgG Target Name MURF2 / TRIM55 Antigen Species Human Immunogen C-HIFSFSWLNSLNE Conjugation Un-conjugated Alternate Names Muscle-specific RING finger protein 2; muRF2; MuRF-2; MURF-2; RNF29; MuRF2; RING finger protein 29; Tripartite motif-containing protein 55 Application Instructions Application Note WB: Recommend incubate at RT for 1h. Approx 85 kDa band observed in Human and Mouse Heart lysates (calculated MW of 60.5 kDa according to NP_908973.1). The observed molecular weight corresponds to earlier findings in literature with the same antibody (Willis et al, Circ Res. 2007 Mar 2;100(4):456-9. PMID: 17272810). Recommended concentration: 0.1-0.3 µg/ml. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Properties Form Liquid Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA Preservative 0.02% Sodium azide Stabilizer 0.5% BSA Concentration 0.5 mg/ml Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated www.arigobio.com 1/2 freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Database links GeneID: 84675 Human Swiss-port # Q9BYV6 Human Background The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein- protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] Resrarch Area Cell Biology and Cellular Response antibody; Gene Regulation antibody Images ARG63312 anti-MURF2 / TRIM55 antibody WB image Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG63312 anti-MURF2 / TRIM55 antibody at 0.1 µg/ml dilution. ARG63312 anti-MURF2 / TRIM55 antibody WB image Western Blot: Mouse heart lysate from MURF2 +/+ or MURF2 -/- mouse stained with ARG63312 anti-MURF2 / TRIM55 antibody at 1:500 dilution. www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).