Contents • May/June 2018 • Volume 9, No. 3

CONTENTS • MAY/JUNE 2018 • VOLUME 9, NO. 3

FEATURED IMAGE Featured image: Adult female deep-sea anglerﬁsh specimens of Melanocetus johnsonii (top) and Cryptopsaras couesii (bottom), collected on DEEPEND Consortium cruises in the Gulf of Mexico. The lure, which holds luminous bacterial symbionts, is present above each individual’s head. (Photo credit: Danté Fenolio, San Antonio Zoo.) [See related article: Hendry et al., mBio 9(3): e01033-18, 2018.] © 2018 Hendry et al. CC-BY 4.0.

LETTERS TO THE EDITOR Multiple Selected Changes May Modulate the Molecular Interaction between e00476-18 Laverania RH5 and Primate Basigin Diego Forni, Chiara Pontremoli, Rachele Cagliani, Uberto Pozzoli, Mario Clerici, Manuela Sironi Inability to Culture Pneumocystis jirovecii e00939-18 Yueqin Liu, Gary A. Fahle, Joseph A. Kovacs

AUTHOR REPLIES Reply to Forni et al., “Multiple Selected Changes May Modulate the Molecular e00917-18 Interaction between Laverania RH5 and Primate Basigin” Lindsey J. Plenderleith, Weimin Liu, Oscar A. MacLean, Yingying Li, Dorothy E. Loy, Sesh A. Sundararaman, Frederic Bibollet-Ruche, Gerald H. Learn, Beatrice H. Hahn, Paul M. Sharp Reply to Das and Berkhout, “How Polypurine Tract Changes in the HIV-1 RNA e00623-18 Genome Can Cause Resistance against the Integrase Inhibitor Dolutegravir” Isabelle Malet, Frédéric Subra, Clémence Richetta, Charlotte Charpentier, Gilles Collin, Diane Descamps, Vincent Calvez, Anne-Geneviève Marcelin, Olivier Delelis Reply to Liu et al., “Inability To Culture Pneumocystis jirovecii” e01030-18 Verena Schildgen, Oliver Schildgen

PERSPECTIVES The Case for an Expanded Concept of Trained Immunity e00570-18 Antonio Cassone Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, e00525-18 and Generalizability in Microbiome Research Patrick D. Schloss

COMMENTARIES Updates in the Language of Histoplasma Biodiversity e00181-18 Pierre Gladieux Toward an Improved Meningococcal Serogroup B Assay e00713-18 Jan Poolman Fighting Antibiotic-Resistant Klebsiella pneumoniae with “Sweet” Immune Targets e00874-18 Roberto Adamo, Immaculada Margarit CONTENTS • MAY/JUNE 2018 • VOLUME 9, NO. 3

Breaching Pathogeographic Barriers by the Bat White-Nose Fungus e00897-18 Johanna Rhodes, Matthew C. Fisher A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus e01001-18 Transcriptome Felicia Goodrum, Shannon McWeeney

OPINION/HYPOTHESIS Could Positive Feedback Enable Bacterial Pheromone Signaling To Coordinate e00098-18 Behaviors in Response to Heterogeneous Environmental Cues? Eric V. Stabb

MINIREVIEWS Hacking the Cell: Network Intrusion and Exploitation by Adenovirus E1A e00390-18 Cason R. King, Ali Zhang, Tanner M. Tessier, Steven F. Gameiro, Joe S. Mymryk Biological Roles Played by Sphingolipids in Dimorphic and Filamentous Fungi e00642-18 Caroline Mota Fernandes, Gustavo H. Goldman, Maurizio Del Poeta Bacterial Quorum Sensing and Microbial Community Interactions e02331-17 Rhea G. Abisado, Saida Benomar, Jennifer R. Klaus, Ajai A. Dandekar, Josephine R. Chandler

OBSERVATIONS Loss of Ethanolamine Utilization in Enterococcus faecalis Increases e00790-18 Gastrointestinal Tract Colonization Karan Gautam Kaval, Kavindra V. Singh, Melissa R. Cruz, Sruti DebRoy, Wade C. Winkler, Barbara E. Murray, Danielle A. Garsin Microscopic and Molecular Evidence of the First Elasmobranch Adomavirus, the e00185-18 Cause of Skin Disease in a Giant Guitarﬁsh, Rhynchobatus djiddensis Jennifer A. Dill, Alvin C. Camus, John H. Leary, Terry Fei Fan Ng The ICP27 Homology Domain of the Human Cytomegalovirus Protein UL69 e01112-18 Adopts a Dimer-of-Dimers Structure Richard B. Tunnicliffe, Richard F. Collins, Hilda D. Ruiz Nivia, Rozanne M. Sandri-Goldin, Alexander P. Golovanov A Novel Method of Serum Resistance by Escherichia coli That Causes Urosepsis e00920-18 Carrie F. Coggon, Andrew Jiang, Kelvin G. K. Goh, Ian R. Henderson, Mark A. Schembri, Timothy J. Wells Shigella-Induced Emergency Granulopoiesis Protects Zebraﬁsh Larvae from e00933-18 Secondary Infection Alexandra R. Willis, Vincenzo Torraca, Margarida C. Gomes, Jennifer Shelley, Maria Mazon-Moya, Alain Filloux, Cristina Lo Celso, Serge Mostowy

RESEARCH ARTICLES Signature Arsenic Detoxiﬁcation Pathways in Halomonas sp. Strain GFAJ-1 e00515-18 Shuangju Wu, Lianrong Wang, Rui Gan, Tong Tong, Hao Bian, Zhiqiang Li, Shiming Du, Zixin Deng, Shi Chen

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Statins Suppress Ebola Virus Infectivity by Interfering with Glycoprotein e00660-18 Processing Punya Shrivastava-Ranjan, Mike Flint, Éric Bergeron, Anita K. McElroy, Payel Chatterjee, César G. Albariño, Stuart T. Nichol, Christina F. Spiropoulou Conductive Particles Enable Syntrophic Acetate Oxidation between Geobacter e00226-18 and Methanosarcina from Coastal Sediments Amelia-Elena Rotaru, Federica Calabrese, Hryhoriy Stryhanyuk, Florin Musat, Pravin Malla Shrestha, Hannah Sophia Weber, Oona L. O. Snoeyenbos-West, Per O. J. Hall, Hans H. Richnow, Niculina Musat, Bo Thamdrup An Attenuated CRISPR-Cas System in Enterococcus faecalis Permits DNA e00414-18 Acquisition Karthik Hullahalli, Marinelle Rodrigues, Uyen Thy Nguyen, Kelli Palmer The Impact of ExoS on Pseudomonas aeruginosa Internalization by Epithelial e00668-18 Cells Is Independent of fleQ and Correlates with Bistability of Type Three Secretion System Gene Expression Abby R. Kroken, Camille K. Chen, David J. Evans, Timothy L. Yahr, Suzanne M. J. Fleiszig SUN2 Modulates HIV-1 Infection and Latency through Association with Lamin e02408-17 A/C To Maintain the Repressive Chromatin Wei-Wei Sun, Shi Jiao, Li Sun, Zhaocai Zhou, Xia Jin, Jian-Hua Wang Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic e00685-18 Activities of Mycobacterium tuberculosis MazF Toxins Akanksha Nigam, Sathish Kumar, Hanna Engelberg-Kulka Selective Packaging in Murine Coronavirus Promotes Virulence by Limiting Type e00272-18 I Interferon Responses Jeremiah Athmer, Anthony R. Fehr, Matthew E. Grunewald, Wen Qu, D. Lori Wheeler, Kevin W. Graepel, Rudragouda Channappanavar, Aimee Sekine, Dana Saud Aldabeeb, Michael Gale, Jr., Mark R. Denison, Stanley Perlman Regulation of Flagellum Biosynthesis in Response to Cell Envelope Stress in e00736-17 Salmonella enterica Serovar Typhimurium Imke Spöring, Sebastian Felgner, Matthias Preuße, Denitsa Eckweiler, Manfred Rohde, Susanne Häussler, Siegfried Weiss, Marc Erhardt Novel Xanthomonas campestris Long-Chain-Specific 3-Oxoacyl-Acyl Carrier e00596-18 Protein Reductase Involved in Diffusible Signal Factor Synthesis Zhe Hu, Huijuan Dong, Jin-Cheng Ma, Yonghong Yu, Kai-Hui Li, Qiao-Qiao Guo, Chao Zhang, Wen-Bin Zhang, Xinyun Cao, John E. Cronan, Haihong Wang Compositional and Functional Differences in the Human Gut Microbiome e00686-18 Correlate with Clinical Outcome following Infection with Wild-Type Salmonella enterica Serovar Typhi Yan Zhang, Arthur Brady, Cheron Jones, Yang Song, Thomas C. Darton, Claire Jones, Christoph J. Blohmke, Andrew J. Pollard, Laurence S. Magder, Alessio Fasano, Marcelo B. Sztein, Claire M. Fraser Ceftazidime Is the Key Diversification and Selection Driver of VIM-Type e02109-17 Carbapenemases Laura Martínez-García, José M. González-Alba, Fernando Baquero, Rafael Cantón, Juan Carlos Galán

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CLAG3 Self-Associates in Malaria Parasites and Quantitatively Determines e02293-17 Nutrient Uptake Channels at the Host Membrane Ankit Gupta, Praveen Balabaskaran-Nina, Wang Nguitragool, Gagandeep S. Saggu, Marc A. Schureck, Sanjay A. Desai Bone Marrow Is a Major Parasite Reservoir in Plasmodium vivax Infection e00625-18 Nicanor Obaldia III, Elamaran Meibalan, Juliana M. Sa, Siyuan Ma, Martha A. Clark, Pedro Mejia, Roberto R. Moraes Barros, William Otero, Marcelo U. Ferreira, James R. Mitchell, Danny A. Milner, Curtis Huttenhower, Dyann F. Wirth, Manoj T. Duraisingh, Thomas E. Wellems, Matthias Marti Repurposing Cytarabine for Treating Primary Effusion Lymphoma by Targeting e00756-18 Kaposi’s Sarcoma-Associated Herpesvirus Latent and Lytic Replications Marion Gruffaz, Shenghua Zhou, Karthik Vasan, Teresa Rushing, Qing Liu Michael, Chu Lu, Jae U. Jung, Shou-Jiang Gao Identification of the Intragenomic Promoter Controlling Hepatitis E Virus e00769-18 Subgenomic RNA Transcription Qiang Ding, Ila Nimgaonkar, Nicholas F. Archer, Yaron Bram, Brigitte Heller, Robert E. Schwartz, Alexander Ploss A High Frequency of HIV-Specific Circulating Follicular Helper T Cells Is e00317-18 Associated with Preserved Memory B Cell Responses in HIV Controllers M. Claireaux, M. Galperin, D. Benati, A. Nouël, M. Mukhopadhyay, J. Klingler, P. de Truchis, D. Zucman, S. Hendou, F. Boufassa, C. Moog, O. Lambotte, L. A. Chakrabarti CD147 Promotes Entry of Pentamer-Expressing Human Cytomegalovirus into e00781-18 Epithelial and Endothelial Cells Adam L. Vanarsdall, Sarah R. Pritchard, Todd W. Wisner, Jing Liu, Ted S. Jardetzky, David C. Johnson Comparative Population Genomics Analysis of the Mammalian Fungal Pathogen e00381-18 Pneumocystis Ousmane H. Cissé, Liang Ma, Da Wei Huang, Pavel P. Khil, John P. Dekker, Geetha Kutty, Lisa Bishop, Yueqin Liu, Xilong Deng, Philippe M. Hauser, Marco Pagni, Vanessa Hirsch, Richard A. Lempicki, Jason E. Stajich, Christina A. Cuomo, Joseph A. Kovacs Comparative Genomics Reveals the Core Gene Toolbox for the Fungus-Insect e00636-18 Symbiosis Yan Wang, Matt Stata, Wei Wang, Jason E. Stajich, Merlin M. White, Jean-Marc Moncalvo A Mechanism of Unidirectional Transformation, Leading to Antibiotic Resistance, e00561-18 Occurs within Nasopharyngeal Pneumococcal Biofilm Consortia Santiago M. Lattar, Xueqing Wu, Jennifer Brophy, Fuminori Sakai, Keith P. Klugman, Jorge E. Vidal CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is e00717-18 Associated with Alterations in Integrin Binding Emma C. Skoog, Vasilios A. Morikis, Miriam E. Martin, Greg A. Foster, Lucy P. Cai, Lori M. Hansen, Beibei Li, Jennifer A. Gaddy, Scott I. Simon, Jay V. Solnick Predictable Phenotypes of Antibiotic Resistance Mutations e00770-18 M. Knopp, D. I. Andersson

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Inducible Lung Epithelial Resistance Requires Multisource Reactive Oxygen e00696-18 Species Generation To Protect against Viral Infections Carson T. Kirkpatrick, Yongxing Wang, Miguel M. Leiva Juarez, Pooja Shivshankar, Jezreel Pantaleón García, Alexandria K. Plumer, Vikram V. Kulkarni, Hayden H. Ware, Fahad Gulraiz, Miguel A. Chavez Cavasos, Gabriela Martinez Zayes, Shradha Wali, Andrew P. Rice, Hongbing Liu, James M. Tour, William K. A. Sikkema, Ana S. Cruz Solbes, Keith A. Youker, Michael J. Tuvim, Burton F. Dickey, Scott E. Evans SAMHD1 Phosphorylation Coordinates the Anti-HIV-1 Response by Diverse e00819-18 Interferons and Tyrosine Kinase Inhibition Matthew A. Szaniawski, Adam M. Spivak, James E. Cox, Jonathan L. Catrow, Timothy Hanley, Elizabeth S. C. P. Williams, Michel J. Tremblay, Alberto Bosque, Vicente Planelles RECON-Dependent Inflammation in Hepatocytes Enhances e00526-18 Listeria monocytogenes Cell-to-Cell Spread Adelle P. McFarland, Thomas P. Burke, Alexie A. Carletti, Rochelle C. Glover, Hannah Tabakh, Matthew D. Welch, Joshua J. Woodward Bat Caliciviruses and Human Noroviruses Are Antigenically Similar and Have e00869-18 Overlapping Histo-Blood Group Antigen Binding Profiles Jacob F. Kocher, Lisa C. Lindesmith, Kari Debbink, Anne Beall, Michael L. Mallory, Boyd L. Yount, Rachel L. Graham, Jeremy Huynh, J. Edward Gates, Eric F. Donaldson, Ralph S. Baric Role of Severe Acute Respiratory Syndrome Coronavirus Viroporins E, 3a, and 8a e02325-17 in Replication and Pathogenesis Carlos Castaño-Rodriguez, Jose M. Honrubia, Javier Gutiérrez-Álvarez, Marta L. DeDiego, Jose L. Nieto-Torres, Jose M. Jimenez-Guardeño, Jose A. Regla-Nava, Raul Fernandez-Delgado, Carmina Verdia-Báguena, Maria Queralt-Martín, Grazyna Kochan, Stanley Perlman, Vicente M. Aguilella, Isabel Sola, Luis Enjuanes A Single-Domain Response Regulator Functions as an Integrating Hub To e00809-18 Coordinate General Stress Response and Development in Alphaproteobacteria C. Lori, A. Kaczmarczyk, I. de Jong, U. Jenal Loss of Upc2p-Inducible ERG3 Transcription Is Sufficient To Confer Niche-Specific e00225-18 Azole Resistance without Compromising Candida albicans Pathogenicity Arturo Luna-Tapia, Hubertine M. E. Willems, Josie E. Parker, Hélène Tournu, Katherine S. Barker, Andrew T. Nishimoto, P. David Rogers, Steven L. Kelly, Brian M. Peters, Glen E. Palmer Conserved Responses in a War of Small Molecules between a Plant-Pathogenic e00820-18 Bacterium and Fungi Joseph E. Spraker, Philipp Wiemann, Joshua A. Baccile, Nandhitha Venkatesh, Julia Schumacher, Frank C. Schroeder, Laura M. Sanchez, Nancy P. Keller Wolbachia wStri Blocks Zika Virus Growth at Two Independent Stages of Viral e00738-18 Replication M. J. Schultz, A. L. Tan, C. N. Gray, S. Isern, S. F. Michael, H. M. Frydman, J. H. Connor

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Structural Alteration of Gut Microbiota during the Amelioration of Human Type e02392-17 2 Diabetes with Hyperlipidemia by Metformin and a Traditional Chinese Herbal Formula: a Multicenter, Randomized, Open Label Clinical Trial Xiaolin Tong, Jia Xu, Fengmei Lian, Xiaotong Yu, Yufeng Zhao, Lipeng Xu, Menghui Zhang, Xiyan Zhao, Jian Shen, Shengping Wu, Xiaoyan Pang, Jiaxing Tian, Chenhong Zhang, Qiang Zhou, Linhua Wang, Bing Pang, Feng Chen, Zhiping Peng, Jing Wang, Zhong Zhen, Chao Fang, Min Li, Limei Chen, Liping Zhao A New Family of Capsule Polymerases Generates Teichoic Acid-Like Capsule e00641-18 Polymers in Gram-Negative Pathogens Christa Litschko, Davide Oldrini, Insa Budde, Monika Berger, Jochen Meens, Rita Gerardy-Schahn, Francesco Berti, Mario Schubert, Timm Fiebig Fungal Isocyanide Synthases and Xanthocillin Biosynthesis in e00785-18 Aspergillus fumigatus Fang Yun Lim, Tae Hyung Won, Nicholas Raffa, Joshua A. Baccile, Jen Wisecaver, Antonis Rokas, Frank C. Schroeder, Nancy P. Keller Rational Design of Biosafety Level 2-Approved, Multidrug-Resistant Strains of e00938-18 Mycobacterium tuberculosis through Nutrient Auxotrophy Catherine Vilchèze, Jacqueline Copeland, Tracy L. Keiser, Torin Weisbrod, Jacqueline Washington, Paras Jain, Adel Malek, Brian Weinrick, William R. Jacobs, Jr. Increased Usage of Antiseptics Is Associated with Reduced Susceptibility in e00894-18 Clinical Isolates of Staphylococcus aureus Katherine Hardy, Katie Sunnucks, Hannah Gil, Sahida Shabir, Eleftheria Trampari, Peter Hawkey, Mark Webber Genomic and Geographic Context for the Evolution of High-Risk Carbapenem- e00542-18 Resistant Enterobacter cloacae Complex Clones ST171 and ST78 Angela Gomez-Simmonds, Medini K. Annavajhala, Zheng Wang, Nenad Macesic, Yue Hu, Marla J. Giddins, Aidan O’Malley, Nora C. Toussaint, Susan Whittier, Victor J. Torres, Anne-Catrin Uhlemann AupA and AupB Are Outer and Inner Membrane Proteins Involved in Alkane e00520-18 Uptake in Marinobacter hydrocarbonoclasticus SP17 Julie Mounier, Florence Hakil, Priscilla Branchu, Muriel Naïtali, Philippe Goulas, Pierre Sivadon, Régis Grimaud Emergence and Evolution of Novel Reassortant Inﬂuenza A Viruses in Canines in e00909-18 Southern China Ying Chen, Nídia S. Trovão, Guojun Wang, Weifeng Zhao, Ping He, Huabo Zhou, Yanning Mo, Zuzhang Wei, Kang Ouyang, Weijian Huang, Adolfo García-Sastre, Martha I. Nelson Candida albicans-Induced Epithelial Damage Mediates Translocation through e00915-18 Intestinal Barriers Stefanie Allert, Toni M. Förster, Carl-Magnus Svensson, Jonathan P. Richardson, Tony Pawlik, Betty Hebecker, Sven Rudolphi, Marc Juraschitz, Martin Schaller, Mariana Blagojevic, Joachim Morschhäuser, Marc Thilo Figge, Ilse D. Jacobsen, Julian R. Naglik, Lydia Kasper, Selene Mogavero, Bernhard Hube DDX41 Recognizes RNA/DNA Retroviral Reverse Transcripts and Is Critical for In e00923-18 Vivo Control of Murine Leukemia Virus Infection Spyridon Stavrou, Alexya N. Aguilera, Kristin Blouch, Susan R. Ross

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Leveraging Existing 16S rRNA Gene Surveys To Identify Reproducible Biomarkers e00630-18 in Individuals with Colorectal Tumors Marc A. Sze, Patrick D. Schloss Engineering Nitrogen Fixation Activity in an Oxygenic Phototroph e01029-18 Deng Liu, Michelle Liberton, Jingjie Yu, Himadri B. Pakrasi, Maitrayee Bhattacharyya-Pakrasi Novel Partitivirus Enhances Virulence of and Causes Aberrant Gene Expression in e00947-18 Talaromyces marneffei Susanna K. P. Lau, George C. S. Lo, Franklin W. N. Chow, Rachel Y. Y. Fan, James J. Cai, Kwok-Yung Yuen, Patrick C. Y. Woo Sequence Variation of Rare Outer Membrane Protein ␤-Barrel Domains in Clinical e01006-18 Strains Provides Insights into the Evolution of Treponema pallidum subsp. pallidum, the Syphilis Spirochete Sanjiv Kumar, Melissa J. Caimano, Arvind Anand, Abhishek Dey, Kelly L. Hawley, Morgan E. LeDoyt, Carson J. La Vake, Adriana R. Cruz, Lady G. Ramirez, Lenka Pašteˇková, Irina Bezsonova, David Šmajs, Juan C. Salazar, Justin D. Radolf Reassessing the Role of Type II Toxin-Antitoxin Systems in Formation of e00640-18 Escherichia coli Type II Persister Cells Frédéric Goormaghtigh, Nathan Fraikin, Marta Putrinš, Thibaut Hallaert, Vasili Hauryliuk, Abel Garcia-Pino, Andreas Sjödin, Sergo Kasvandik, Klas Udekwu, Tanel Tenson, Niilo Kaldalu, Laurence Van Melderen Genomic Insight into Symbiosis-Induced Insect Color Change by a Facultative e00890-18 Bacterial Endosymbiont, “Candidatus Rickettsiella viridis” Naruo Nikoh, Tsutomu Tsuchida, Taro Maeda, Katsushi Yamaguchi, Shuji Shigenobu, Ryuichi Koga, Takema Fukatsu Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary-Phase Autolysis e00648-18 Resulting in Toxin Release in Clostridium difﬁcile Sandra Wydau-Dematteis, Imane El Meouche, Pascal Courtin, Audrey Hamiot, René Lai-Kuen, Bruno Saubaméa, François Fenaille, Marie-José Butel, Jean-Louis Pons, Bruno Dupuy, Marie-Pierre Chapot-Chartier, Johann Peltier Mycobacterium tuberculosis Pst/SenX3-RegX3 Regulates Membrane Vesicle e00778-18 Production Independently of ESX-5 Activity Dylan W. White, Sarah R. Elliott, Evan Odean, Lynne T. Bemis, Anna D. Tischler Global Proteomic Changes Induced by the Epstein-Barr Virus Oncoproteins e00959-18 Latent Membrane Protein 1 and 2A Robert M. DeKroon, Harsha P. Gunawardena, Rachel Edwards, Nancy Raab-Traub Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric e00546-18 Meningococcal Sepsis Noortje de Haan, Navin P. Boeddha, Ebru Ekinci, Karli R. Reiding, Marieke Emonts, Jan A. Hazelzet, Manfred Wuhrer, Gertjan J. Driessen CRISPR Screen Reveals that EHEC’s T3SS and Shiga Toxin Rely on Shared Host e01003-18 Factors for Infection Alline R. Pacheco, Jacob E. Lazarus, Brandon Sit, Stefanie Schmieder, Wayne I. Lencer, Carlos J. Blondel, John G. Doench, Brigid M. Davis, Matthew K. Waldor

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Four Molybdenum-Dependent Steroid C-25 Hydroxylases: Heterologous e00694-18 Overproduction, Role in Steroid Degradation, and Application for

25-Hydroxyvitamin D3 Synthesis Christian Jacoby, Jens Eipper, Markus Warnke, Oliver Tiedt, Mario Mergelsberg, Hans-Joachim Stärk, Birgit Daus, Zaira Martín-Moldes, María Teresa Zamarro, Eduardo Díaz, Matthias Boll Escherichia coli ZipA Organizes FtsZ Polymers into Dynamic Ring-Like e01008-18 Protoﬁlament Structures Marcin Krupka, Marta Sobrinos-Sanguino, Mercedes Jiménez, Germán Rivas, William Margolin How, When, and Where Relic DNA Affects Microbial Diversity e00637-18 J. T. Lennon, M. E. Muscarella, S. A. Placella, B. K. Lehmkuhl High-Throughput Screening of Type III Secretion Determinants Reveals a Major e01050-18 Chaperone-Independent Pathway Nadja Heinz Ernst, Analise Z. Reeves, Julia E. Ramseyer, Cammie F. Lesser Cytokinin Signaling in Mycobacterium tuberculosis e00989-18 Marie I. Samanovic, Hao-Chi Hsu, Marcus B. Jones, Victoria Jones, Michael R. McNeil, Samuel H. Becker, Ashley T. Jordan, Miroslav Strnad, Changcheng Xu, Mary Jackson, Huilin Li, K. Heran Darwin The PGRS Domain of Mycobacterium tuberculosis PE_PGRS Protein Rv0297 Is e01017-18 Involved in Endoplasmic Reticulum Stress-Mediated Apoptosis through Toll-Like Receptor 4 Sonam Grover, Tarina Sharma, Yadvir Singh, Sakshi Kohli, Manjunath P., Aditi Singh, Torsten Semmler, Lothar H. Wieler, Karsten Tedin, Nasreen Z. Ehtesham, Seyed E. Hasnain The Pearling Transition Provides Evidence of Force-Driven Endosomal Tubulation e01083-18 during Salmonella Infection Yunfeng Gao, Christoph Spahn, Mike Heilemann, Linda J. Kenney Regulation of Aspergillus nidulans CreA-Mediated Catabolite Repression by the e00840-18 F-Box Proteins Fbx23 and Fbx47 Leandro José de Assis, Mevlut Ulas, Laure Nicolas Annick Ries, Nadia Ali Mohamed El Ramli, Ozlem Sarikaya-Bayram, Gerhard H. Braus, Ozgur Bayram, Gustavo Henrique Goldman In Vitro Reconstitution of Functional Type III Protein Export and Insights into e00988-18 Flagellar Assembly Hiroyuki Terashima, Akihiro Kawamoto, Chinatsu Tatsumi, Keiichi Namba, Tohru Minamino, Katsumi Imada A Novel Adaptation Mechanism Underpinning Algal Colonization of a Nuclear e02395-17 Fuel Storage Pond Victoria E. MeGraw, Ashley R. Brown, Christopher Boothman, Royston Goodacre, Katherine Morris, David Sigee, Lizzie Anderson, Jonathan R. Lloyd

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Ongoing Transposon-Mediated Genome Reduction in the Luminous Bacterial e01033-18 Symbionts of Deep-Sea Ceratioid Anglerﬁshes Tory A. Hendry, Lindsay L. Freed, Dana Fader, Danté Fenolio, Tracey T. Sutton, Jose V. Lopez Introduction of Large Sequence Inserts by CRISPR-Cas9 To Create Pathogenicity e00567-18 Mutants in the Multinucleate Filamentous Pathogen Sclerotinia sclerotiorum Jingtao Li, Yanhua Zhang, Yucheng Zhang, Pei-Ling Yu, Hongyu Pan, Jeffrey A. Rollins Long-Term In Vitro Culture of the Syphilis Spirochete Treponema pallidum subsp. e01153-18 pallidum Diane G. Edmondson, Bo Hu, Steven J. Norris Identiﬁcation of Host Factors Involved in Human Cytomegalovirus Replication, e00716-18 Assembly, and Egress Using a Two-Step Small Interfering RNA Screen Dominique McCormick, Yao-Tang Lin, Finn Grey Trichomonas vaginalis Macrophage Migration Inhibitory Factor Mediates Parasite e00910-18 Survival during Nutrient Stress Yi-Pei Chen, Olivia Twu, Patricia J. Johnson The Rickettsial Ankyrin Repeat Protein 2 Is a Type IV Secreted Effector That e00975-18 Associates with the Endoplasmic Reticulum Stephanie S. Lehman, Nicholas F. Noriea, Karin Aistleitner, Tina R. Clark, Cheryl A. Dooley, Vinod Nair, Simran Jeet Kaur, M. Sayeedur Rahman, Joseph J. Gillespie, Abdu F. Azad, Ted Hackstadt

ERRATA Erratum for May and Silhavy, “The Escherichia coli Phospholipase PldA Regulates e00718-18 Outer Membrane Homeostasis via Lipid Signaling” Kerrie L. May, Thomas J. Silhavy Erratum for Xu et al., “Disruption of Early Tumor Necrosis Factor Alpha Signaling e01039-18 Prevents Classical Activation of Dendritic Cells in Lung-Associated Lymph Nodes and Development of Protective Immunity against Cryptococcal Infection” Jintao Xu, Alison J. Eastman, Adam Flaczyk, Lori M. Neal, Guolei Zhao, Jacob Carolan, Antoni N. Malachowski, Valerie R. Stolberg, Mohammed Yosri, Stephen W. Chensue, Jeffrey L. Curtis, John J. Osterholzer, Michal A. Olszewski Erratum for Diago-Navarro et al., “Novel, Broadly Reactive Anticapsular Antibodies e01005-18 against Carbapenem-Resistant Klebsiella pneumoniae Protect from Infection” Elizabeth Diago-Navarro, Michael P. Motley, Gonzalo Ruiz-Peréz, Winnie Yu, Julianne Austin, Bruna M. S. Seco, Guozhi Xiao, Aniska Chikhalya, Peter H. Seeberger, Bettina C. Fries Erratum for Gupta et al., “CLAG3 Self-Associates in Malaria Parasites and e01181-18 Quantitatively Determines Nutrient Uptake Channels at the Host Membrane” Ankit Gupta, Praveen Balabaskaran-Nina, Wang Nguitragool, Gagandeep S. Saggu, Marc A. Schureck, Sanjay A. Desai

AUTHOR CORRECTION Correction for Olsen et al., “Polyamines and Hypusination Are Required for e01065-18 Ebolavirus Gene Expression and Replication” Michelle E. Olsen, Claire Marie Filone, Dan Rozelle, Chad E. Mire, Krystle N. Agans, Lisa Hensley, John H. Connor

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