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RESEARCH ARTICLE Anti-Glioma Effect of Pseudosynanceia
DOI:10.31557/APJCP.2021.22.7.2295 Effect of Pseudosynanceia Melanostigma Venom on Glioblastoma Cells RESEARCH ARTICLE Editorial Process: Submission:02/14/2021 Acceptance:07/15/2021 Anti-Glioma Effect of Pseudosynanceia Melanostigma Venom on Isolated Mitochondria from Glioblastoma Cells Maral Ramezani, Fatemeh Samiei, Jalal Pourahmad* Abstract Background: Glioblastoma is the most common primary malignant tumor of the central nervous system that occurs in the spinal cord or brain. Pseudosynanceia Melanostigma is a venomous stonefish in the Persian Gulf, which our knowledge about is little. This study’s goal is to investigate the toxicity of stonefish crude venom on mitochondria isolated from U87 cells. Methods: In the first stage, we extracted venom stonefish and then isolated mitochondria have exposed to different concentrations of venom. Finally, mitochondrial toxicity parameters (Succinate dehydrogenase (SDH) activity, Reactive oxygen species (ROS), cytochrome c release, Mitochondrial Membrane Potential (MMP), and mitochondrial swelling) have evaluated. Results: To determine mitochondrial parameters, we used 115, 230, and 460 µg/ml concentrations. The results of our study show that the venom of stonefish selectively increases upstream parameters of apoptosis such as mitochondrial swelling, cytochrome c release, MMP collapse and ROS. Conclusion: This study suggests that Pseudosynanceia Melanostigma crude venom has selectively caused toxicity by increasing active mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma. Keywords: Mitochondria- glioblastoma- fish venoms- cell line- tumor- toxicity-Persian Gulf Asian Pac J Cancer Prev, 22 (7), 2295-2302 Introduction freshwater or saltwater. Marine animals most commonly used for animals live in saltwater, i.e. in oceans, seas, Glioblastoma multiform is the most common group of etc. -
Spider Bites
Infectious Disease Epidemiology Section Office of Public Health, Louisiana Dept of Health & Hospitals 800-256-2748 (24 hr number) www.infectiousdisease.dhh.louisiana.gov SPIDER BITES Revised 6/13/2007 Epidemiology There are over 3,000 species of spiders native to the United States. Due to fragility or inadequate length of fangs, only a limited number of species are capable of inflicting noticeable wounds on human beings, although several small species of spiders are able to bite humans, but with little or no demonstrable effect. The final determination of etiology of 80% of suspected spider bites in the U.S. is, in fact, an alternate diagnosis. Therefore the perceived risk of spider bites far exceeds actual risk. Tick bites, chemical burns, lesions from poison ivy or oak, cutaneous anthrax, diabetic ulcer, erythema migrans from Lyme disease, erythema from Rocky Mountain Spotted Fever, sporotrichosis, Staphylococcus infections, Stephens Johnson syndrome, syphilitic chancre, thromboembolic effects of Leishmaniasis, toxic epidermal necrolyis, shingles, early chicken pox lesions, bites from other arthropods and idiopathic dermal necrosis have all been misdiagnosed as spider bites. Almost all bites from spiders are inflicted by the spider in self defense, when a human inadvertently upsets or invades the spider’s space. Of spiders in the United States capable of biting, only a few are considered dangerous to human beings. Bites from the following species of spiders can result in serious sequelae: Louisiana Office of Public Health – Infectious Disease Epidemiology Section Page 1 of 14 The Brown Recluse: Loxosceles reclusa Photo Courtesy of the Texas Department of State Health Services The most common species associated with medically important spider bites: • Physical characteristics o Length: Approximately 1 inch o Appearance: A violin shaped mark can be visualized on the dorsum (top). -
The Bug Beneath the Bathing Suit: a Case Report and Discussion of Seabather’S Eruption Versus Cutaneous Larva Migrans
The bug beneath the bathing suit: A case report and discussion of seabather’s eruption versus cutaneous larva migrans Andrew Jensen, BS,* Marcus Goodman, DO, FAOCD** *Medical Student, 4th year, Philadelphia College of Osteopathic Medicine - Georgia Campus, Suwanee, GA **Dermatology Residency Program Director, PCOM/North Fulton Hospital Medical Campus, Roswell, GA Abstract Seabather’s eruption is an important differential diagnosis when a patient who has recently swum in a subtropical ocean presents with a pruritic rash in the distribution of their swimwear. Treatment with systemic corticosteroids is indicated in severe cases and can successfully reduce symptoms. Oral steroid therapy in general has proven to be an effective treatment for many acute and chronic diseases but has long been associated with increased risk for infections. In this report, we present an atypical case of cutaneous larva migrans and discuss its clinical unmasking after systemic steroid treatment was given for an initial diagnosis of seabather’s eruption. Introduction Case Report Figure 2 Seabather’s eruption is a benign, superficial A 52-year-old female presented to her reaction to toxins from marine-animal larvae. dermatologist complaining of an itchy rash on It is the most common marine-related problem her groin and upper leg for one week. The patient in the waters south of the United States.1 stated she recently traveled to Mexico, where she It was reported in Florida as early as 1903 spent several days on the beach and swimming in as a “rash which set up an intense itching” the ocean. Physical exam revealed erythematous, shortly after bathing in ocean water.2 In 1949, edematous papules on her lower abdomen and Sams postulated the eruption was caused by groin, assuming a location directly beneath her “some living, microorganism, in the nature of swimsuit (Figure 1). -
Violent Incidents Between Humans and Orcas in Captivity
Violent incidents between humans and orcas in captivity Several accounts of violent incidents with humans have appeared in books and news clips, with little information on the dates or details of those incidents. Other descriptions have made headlines, and some were captured on video tape. There are also anecdotal reports of incidents that were never officially documented. NO. DATE AQUARIUM WHALEs INCIDENT SOURCE early years New York When water level was lowered for pool cleaning, young female Lupa sent Edward R. Riciuti, , New #1 1968 Lupa York, Walker & Co., 1973, Aquarium, USA trainers scrambling from the pool, snapping her jaws threatening. pp. 227-228. Edward R. Riciuti, Killers of the Sea, New York, Young male Cuddles became so increasingly aggressive, having a hold of at Walker & Co., 1973, pp. Flamingo Park, least two trainers, that keepers had to clean the pool from the protection of a 227-228; Reading #2 1969-1970 Cuddles England shark cage. Cuddles also dragged keeper Don Robinson into the pool when he Eagle, August 15, was at Dudley Zoo but that was possibly a PR stunt. 1971; Doug Cartlidge, personal communication, March 2010. Karen Pryor writes, "I have since heard... of at least one killer whale which Karen Pryor, Lads Before the Wind, New York, #3 1970s unknown unknown launched an unprovoked attack on a favorite trainer, in normal circumstances, Harper & Row, 1976, p. savaged him very badly, and nearly killed him." 220. Vancouver Trainer Doug Pemberton described young female Skana as the dominant Cranky killer whales put trainers through their #4 1970's Aquarium, Skana animal in the pool. -
Awareness, Prevention and Treatment of World-Wide Marine Stings and Bites
Awareness, Prevention and Treatment of world-wide marine stings and bites Dr Peter Fenner Honorary Medical Officer, Surf Life Saving Australia International Life Saving Federation Medical/Rescue Conference Proceedings September 1997 Abstract The most common world-wide first aid treatment used by the average lifesaver/lifeguard is the treatment of marine envenomation, especially the treatment of jellyfish stings. It is important to use the correct first aid treatment for each type of envenomation. This study provides a simplified protocol for: - 1. Awareness of the geographical distribution and possibilities of envenomation enabling: - 2. Preventative strategies to reduce morbidity and mortality from marine envenomation 3. First aid treatment of marine envenomation by jellyfish or other marine animals This discussion is based on protocols developed for Surf Life Saving Australia and other first aid providers in Australia over the past ten years. Their success has been proven by a 30% reduction in the number of stings over the past 10 years (statistics from the author’s records). Information for this article has been taken from: - 1. Venomous and poisonous marine animals: a medical and biological handbook produced by Surf Life Saving Queensland 2. The global problem of cnidarian stinging. MD Thesis by the author for the University of London. Introduction The global problem of marine envenomation is not fully appreciated. Each year hundreds of deaths occur from poisoning (by ingestion or eating) or by envenomation (stinging by jellyfish, or biting by venomous marine animals). The morbidity is even greater with jellyfish stings world-wide being numbered in their millions. Each summer it is estimated that up to half a million stings occur on the east coast of the United States from the Portuguese man-o’-war (Physalia physalis). -
Population Structures and Levels of Connectivity for Scyphozoan and Cubozoan Jellyfish
diversity Review Population Structures and Levels of Connectivity for Scyphozoan and Cubozoan Jellyfish Michael J. Kingsford * , Jodie A. Schlaefer and Scott J. Morrissey Marine Biology and Aquaculture, College of Science and Engineering and ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD 4811, Australia; [email protected] (J.A.S.); [email protected] (S.J.M.) * Correspondence: [email protected] Abstract: Understanding the hierarchy of populations from the scale of metapopulations to mesopop- ulations and member local populations is fundamental to understanding the population dynamics of any species. Jellyfish by definition are planktonic and it would be assumed that connectivity would be high among local populations, and that populations would minimally vary in both ecological and genetic clade-level differences over broad spatial scales (i.e., hundreds to thousands of km). Although data exists on the connectivity of scyphozoan jellyfish, there are few data on cubozoans. Cubozoans are capable swimmers and have more complex and sophisticated visual abilities than scyphozoans. We predict, therefore, that cubozoans have the potential to have finer spatial scale differences in population structure than their relatives, the scyphozoans. Here we review the data available on the population structures of scyphozoans and what is known about cubozoans. The evidence from realized connectivity and estimates of potential connectivity for scyphozoans indicates the following. Some jellyfish taxa have a large metapopulation and very large stocks (>1000 s of km), while others have clade-level differences on the scale of tens of km. Data on distributions, genetics of medusa and Citation: Kingsford, M.J.; Schlaefer, polyps, statolith shape, elemental chemistry of statoliths and biophysical modelling of connectivity J.A.; Morrissey, S.J. -
A Guide to Harmful and Toxic Creatures in the Goa of Jordan
Published by the Royal Marine Conservation Society of Jordan. P. O. Box 831051, Abdel Aziz El Thaalbi St., Shmesani 11183. Amman Copyright: © The Royal Marine Conservation Society of Jordan Reproduction of this publication for educational and other non- commercial purposes is authorized without prior written approval from the copyright holder provided the source is fully acknowledged. ISBN: 978-9957-8740-1-8 Deposit Number at the National Library: 2619/6/2016 Citation: Eid, E and Al Tawaha, M. (2016). A Guide to Harmful and Toxic Creature in the Gulf of Aqaba of Jordan. The Royal Marine Conservation Society of Jordan. ISBN: 978-9957-8740-1-8. Pp 84. Material was reviewed by Dr Nidal Al Oran, International Research Center for Water, Environment and Energy\ Al Balqa’ Applied University,and Dr. Omar Attum from Indiana University Southeast at the United State of America. Cover page: Vlad61; Shutterstock Library All photographs used in this publication remain the property of the original copyright holder, and it should not be reproduced or used in other contexts without permission. 1 Content Index of Creatures Described in this Guide ......................................................... 5 Preface ................................................................................................................ 6 Part One: Introduction ......................................................................................... 8 1.1 The Gulf of Aqaba; Jordan ......................................................................... 8 1.2 Aqaba; -
Dangerous Creatures of the Maltese Sea: Injuries and Treatment - Part 2
REVIEW ARTICLE Dangerous creatures of the Maltese sea: Injuries and treatment - Part 2 DR DAVID JAMES SAMMUT ABSTRACT deeper than the epidermis. The common Mediterranean This is the second of a two-part article intended sea urchin does not possess any venom in its spines, thus to give information about different organisms which injury is often of a minor nature. inhabit the Maltese sea and which are potentially harmful. Doctors working in the primary health setup Treatment and sometimes also in secondary care are often faced Treatment options vary. Embedded spines can either with injuries related to these organisms. The nature of be removed immediately, after a few days, or left to come the injury and its treatment is then discussed. Treatment out spontaneously. Any large projecting spines should however is not evidenced based as little if any studies be carefully removed with tweezers (Drobina, 2008). have been conducted in this field of medicine. After the Deeper spines are more difficult to remove. Removing first article considered venomous organisms, this second them immediately is often time consuming, painful and article will review sea creatures that may cause injury may cause more trauma to the site. Superficial spines are through bites, spines and electricity. best left undisturbed, as they will eventually be rejected spontaneously as the epidermis renews itself. Deeper KEY WORDS spines will come out easier if left for a few days before Bites, moray eel bites, sea urchin puncture attempting to remove them. The skin over the spine is broken with a needle and the spine is easily removed by INTRODUCTION applying pressure at the sides of the spine. -
A Summary of the Effects of Captivity on Orcas
A Summary of the Effects of Captivity on Orcas PEOPLE FOR THE ETHICAL TREATMENT OF ANIMALS Contents The Eff ects of Captivity on Tilikum and Orcas Generally at SeaWorld…………..................................…………......3 I. Orcas Are Extremely Intelligent Mammals Whose Brains Are Highly Developed in Areas Responsible for Complex Cognitive Functions, Including Self-Awareness, Social Cognition, Culture, and Language …………………………………………...............................................................................................…...4 II. Tilikum Is Deprived of Every Facet of His Culture and the Opportunity to Engage in Natural Behavior, Causing Extreme Stress and Suff ering….…………….….......................................................5 A. The Tanks at SeaWorld Provide Inadequate Space and Result in Stress……….…...........................5 B. SeaWorld’s Constant Manipulation of Tilikum’s Social Structure Results in Stress.................7 C. The Tanks at SeaWorld Create a Distressing Acoustic Environment…….………..….........................9 III. The Stressors of the Captive Environment at SeaWorld Result in Aggressiveness, Self- Injury, and Other Physical and Behavioral Abnormalities………………….……..............................................10 A. Aggression Between Orcas and Between Orcas and Humans……..……………..............................……10 B. Stereotypic Behavior………………….……………………………………….......................................................................….…..13 1. Painful Dental Problems Caused by Chewing Metal Gates and Concrete Tanks.....14 2. -
Whale Sharks of the Western Caribbean: an Overview of Current Research and Conservation Efforts and Future Needs for Effective Management of the Species
Gulf and Caribbean Research Vol 19(2), 149–159, 2007 Manuscript received December 26, 2006; accepted May 11, 2007 WHALE SHARKS OF THE WESTERN CARIBBEAN: AN overview OF CURRENT RESEARCH AND conservation efforts AND FUTURE NEEDS FOR EFFECTIVE management OF THE SPECIES Rachel T. Graham Wildlife Conservation Society, PO Box 37, Punta Gorda, Belize, E-mail [email protected] ABSTRACT Whale sharks (Rhincodon typus) are seasonal visitors to four sites in the Western Caribbean, 3 of which are encompassed by the Mesoamerican Barrier Reef. Predictable encounters with the world’s largest fish have raised this species’ profile globally and led to several research and conservation efforts that aim to elucidate the need for information for the species management and balance the growing demand for highly lucrative encounter tour- ism. Tagging studies have demonstrated that the whale shark population is relatively small and likely forms a single population. Individuals move throughout the region between 3 of 4 known feeding sites and are capable of timing their movements to pulses of productivity. Whale shark tourism’s dramatic growth has led to a range of protective measures and scientific studies both precautionary and reactionary that require better harmonization throughout the region to be effective. This paper will provide an overview of the status of whale shark research and conservation efforts in the Western Caribbean and identify future management needs to minimize anthropogenic impacts and enable continued whale shark visitation at key feeding sites. RESUMEN Los tiburones ballenas son visitantes estaciónales a cuatro sitios en el Caribe occidental, tres de los cuales se ubican en el arrecife Mesoamericano. -
Proceedings of the Twenty-Ninth Annual Symposium on Sea Turtle Biology and Conservation
NOAA Technical Memorandum NMFS-SEFSC-630 PROCEEDINGS OF THE TWENTY-NINTH ANNUAL SYMPOSIUM ON SEA TURTLE BIOLOGY AND CONSERVATION 17 to 19 February 2009 Brisbane, Queensland, Australia Compiled by: Lisa Belskis, Mike Frick, Aliki Panagopoulou, ALan Rees, & Kris Williams U.S. DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration NOAA Fisheries Service Southeast Fisheries Science Center 75 Virginia Beach Drive Miami, Florida 33149 May 2012 NOAA Technical Memorandum NMFS-SEFSC-630 PROCEEDINGS OF THE TWENTY-NINTH ANNUAL SYMPOSIUM ON SEA TURTLE BIOLOGY AND CONSERVATION 17 to 19 February 2009 Brisbane, Queensland, Australia Compiled by: Lisa Belskis, Mike Frick, Aliki Panagopoulou, ALan Rees, Kris Williams U.S. DEPARTMENT OF COMMERCE John Bryson, Secretary NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION Dr. Jane Lubchenco, Under Secretary for Oceans and Atmosphere NATIONAL MARINE FISHERIES SERVICE Samuel Rauch III, Acting Assistant Administrator for Fisheries May 2012 This Technical Memorandum is used for documentation and timely communication of preliminary results, interim reports, or similar special-purpose information. Although the memoranda are not subject to complete formal review, editorial control or detailed editing, they are expected to reflect sound professional work. NOTICE The NOAA Fisheries Service (NMFS) does not approve, recommend or endorse any proprietary product or material mentioned in this publication. No references shall be made to NMFS, or to this publication furnished by NMFS, in any advertising or sales promotion which would indicate or imply that NMFS approves, recommends or endorses any proprietary product or material herein or which has as its purpose any intent to cause directly or indirectly the advertised product to be use or purchased because of NMFS promotion. -
Investigative Guidelines: Animal Bites and Rabies
PUBLIC HEALTH DIVISION Acute and Communicable Disease Prevention Animal Bites and Rabies Investigative Guidelines December 2018 1. DISEASE REPORTING 1.1 Purpose of Reporting and Surveillance 1. To assess the risk of rabies in persons bitten or otherwise possibly exposed to recommend rabies post-exposure prophylaxis (RPEP) to those who need it, and to provide counseling and reassurance to those who don’t. 2. As necessary to arrange for the capture and either confinement (10-day observation) of a live dog, cat or ferret, or the laboratory examination of an animal head. This may involve coordination with other agencies, e.g., the Humane Society, county sanitarians, animal control and local law enforcement. 3. To identify zoonotic sources of infection. 1.2 Reporting Requirements 1. Anyone with knowledge of humans being bitten by potentially rabid animals (e.g., physicians, veterinarians, animal control personnel, law-enforcement officials, or animal owners), is required to report such incidents to the Local Health Department (LHD) within one working day. 2. Laboratories: Any confirmed case of rabies in an animal and any suspected or confirmed case of human rabies must be reported immediately (day or night) to the LHD. If the LHD cannot be reached, the Acute and Communicable Disease Prevention (ACDP) Section of Oregon Health Authority (OHA) should be contacted at 971-673-1111. 1.3 Local Health Department Reporting and Follow-Up Responsibilities 1. Investigate all reports of animal bites, on the day of the report whenever possible. 2. Determine, in consultation with OHA on-call staff as necessary, whether the exposure constitutes a significant risk for rabies, in which either empiric RPEP or testing of the animal is to be recommended.