Received: 22 August 2017 | Revised: 11 July 2018 | Accepted: 19 July 2018 DOI: 10.1111/mec.14815 ORIGINAL ARTICLE Alternate life history phases of a common seaweed have distinct microbial surface communities Matthew A. Lemay1,2 | Patrick T. Martone1,2 | Katharine R. Hind1,2 | Sandra C. Lindstrom1,2 | Laura Wegener Parfrey1,2,3 1Department of Botany, University of British Columbia, Vancouver, British Abstract Columbia, Canada Macroalgal life histories are complex, often involving the alternation of distinct free‐ 2Hakai Institute, Heriot Bay, British living life history phases that differ in morphology, longevity and ploidy. The sur- Columbia, Canada 3Department of Zoology, University of faces of marine macroalgae support diverse microbial biofilms, yet the degree of British Columbia, Vancouver, British microbial variation between alternate phases is unknown. We quantified bacterial Columbia, Canada (16S rRNA gene) and microeukaryote (18S rRNA gene) communities on the surface Correspondence of the common intertidal seaweed, Mastocarpus spp., which alternates between Matthew A. Lemay, Hakai Institute, Heriot Bay, British Columbia, Canada. gametophyte (foliose, haploid) and sporophyte (encrusting, diploid) life history Email:
[email protected] phases. A large portion (97%) of bacterial taxa on the surface Mastocarpus was also Funding information present in samples from the environment, indicating that macroalgal surface commu- Tula Foundation nities are largely assembled from the surrounding seawater. Still, changes in the rela- tive abundance of bacterial taxa result in significantly different communities on alternate Mastocarpus life history phases, rocky substrate and seawater at all inter- tidal elevations. For microeukaryote assemblages, only high intertidal samples had significant differences between life history phases although sporophytes were not different from the rocky substrate at this elevation; gametophytes and sporophytes did not differ in microeukaryote communities in the mid and low zones.