DOCSLIB.ORG

IRONMAN Tunes Responses to Iron Deficiency in Concert with Environmental Ph

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
IRONMAN Tunes Responses to Iron Deficiency in Concert with Environmental Ph bioRxiv preprint doi: https://doi.org/10.1101/2021.02.16.431461; this version posted February 17, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 1 Short title: IRONMAN Tunes Iron Deficiency Responses 2 3 Corresponding author details: 4 Wolfgang Schmidt 5 Institute of Plant and Microbial Biology, Academia Sinica, 11529 Taipei, Taiwan 6 E-mail, [email protected] 7 8 9 IRONMAN Tunes Responses to Iron Deficiency in Concert with Environmental pH 10 11 Chandan Kumar Gautama,b,c, Huei-Hsuan Tsaic and Wolfgang Schmidta,c,d,e,1 12 13 aMolecular and Biological Agricultural Sciences Program, Taiwan International Graduate 14 Program, Academia Sinica and National Chung-Hsing University, Taipei 11529, Taiwan 15 bGraduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40227, 16 Taiwan 17 cInstitute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan 18 dBiotechnology Center, National Chung-Hsing University, Taichung 40227, Taiwan 19 eGenome and Systems Biology Degree Program, College of Life Science, National Taiwan 20 University, Taipei 10617, Taiwan 21 22 23 One sentence summary: Ectopic expression of IRONMAN peptides improves growth under 24 iron-limiting conditions by inducing responses to limited iron availability in accordance with the 25 environmental pH. 26 27 The author responsible for distribution of materials integral to the findings presented in this 28 article in accordance with the policy described in the Instructions for Authors 29 (www.plantphysiol.org) is: Wolfgang Schmidt ([email protected]). 30 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.16.431461; this version posted February 17, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 31 AUTHOR CONTRIBUTIONS 32 C.K.G., H.H.T, and W.S. designed the research; C.K.G. and H.H.T performed and analyzed 33 experiments; W.S. and C.K.G. wrote the manuscript. 34 35 This work was supported by a grant from the Ministry of Science and Technology to W.S. 36 (grant No.: 108-2311-B-001 -033 -MY3). 37 1Address correspondence to [email protected] 38 2 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.16.431461; this version posted February 17, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 39 ABSTRACT 40 Iron (Fe) is an essential mineral element which governs the composition of natural plant 41 communities and limits crop yield in agricultural ecosystems due to its extremely low 42 availability in most soils, particularly at alkaline pH. To extract sufficient Fe from the soil under 43 such conditions, some plants including Arabidopsis thaliana secrete Fe-mobilizing 44 phenylpropanoids, which mobilize sparingly soluble Fe hydroxides by reduction and chelation. 45 We show here that ectopic expression of the IRONMAN peptides IMA1 and IMA2 improves 46 growth on calcareous soil by inducing the biosynthesis and secretion of the catecholic coumarin 47 fraxetin (7,8-dihydroxy-6-methoxycoumarin) through increased expression of MYB72 and 48 SCOPOLETIN 8-HYDROXYLASE (S8H), a response which is strictly dependent on elevated 49 environmental pH (pHe). By contrast, transcription of the cytochrome P450 family protein 50 CYP82C4, catalyzing the subsequent hydroxylation of fraxetin to sideretin, which forms less 51 stable complexes with iron, was strongly repressed under such conditions. Luciferase reporter 52 assays in transiently transformed protoplasts showed that IMA1/IMA2 peptides are translated 53 and modulate the expression of CYP82C4 and MYB72 by acting as transcriptional coactivators. It 54 is concluded that IMA peptides regulate processes supporting Fe uptake at both acidic and 55 elevated pH by controlling gene expression upstream of or in concert with a putative pHe signal 56 to adapt the plant to the prevailing edaphic conditions. This regulatory pattern confers tolerance 57 to calcareous soils by extending the pH range in which Fe can be efficiently absorbed from the 58 soil. Altering the expression of IMA peptides provides a novel route for generating plants 59 adapted to calcareous soils. 60 3 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.16.431461; this version posted February 17, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 61 INTRODUCTION 62 In natural ecosystems, growth on calcareous, i.e. carbonate-rich, alkaline soils, is restricted to so- 63 called calcicole (chalk-loving) plants, while calcifuge (chalk-fleeing) species are excluded from 64 such habitats (Lee, 1998; Grime and Hodgson, 1969). Among other factors, the ability to thrive 65 on calcareous substrates has been attributed to a particularly efficient strategy to extract iron (Fe) 66 from the soil, an essential mineral nutrient with extremely limited solubility in most conditions. 67 In aerated soils, alkalinity decreases the availability of Fe to levels that are several orders of 68 magnitude below the requirement of the plants (Lindsay and Schwab, 1983; Tyler, 1996). 69 Species that are not well adapted to calcareous soils, as for example crops such as fruit trees and 70 soybean or taxa which originate from acidic habitats, develop lime-induced chlorosis caused by 71 insufficient uptake, maldistribution, or immobilization of Fe (Zohlen and Tyler, 2003). 72 The underlying causes for the superior Fe acquisition efficiency of calcicole species still 73 remain largely enigmatic. Generally, gramineous species (Poaceae) have been regarded as being 74 more Fe-efficient under alkaline condition than non-grasses, a distinction that has been 75 associated with the mechanism by which Fe is mobilized by grass roots. In contrast to all other 76 land plants, grasses have adopted a chelation-based Fe uptake system that relies on the synthesis 77 and secretion of Fe-chelating phytosiderophores, a mechanism that has been designated as 78 strategy II (Römheld and Marschner, 1986). Phytosiderophores are mugineic acid derivates that 79 form soluble complexes with ferric Fe, which are fairly stable over a wide pH range (Shi et al., 80 1988). Conversely, non-grasses have adopted an Fe acquisition strategy which depends on the 81 orchestrated action of enzymatic reduction of ferric chelates, H+-ATPase-mediated acidification 82 of the rhizosphere, and secretion of low-molecular-weight Fe-mobilizing compounds such as 83 flavins or phenylpropanoids, a system that is referred to as strategy I (Römheld and Marschner, 84 1986). In Arabidopsis, chelated ferric Fe is reduced by the plasma membrane-bound FERRIC 85 REDUCTION OXIDASE2 (FRO2; Robinson et al., 1999) and the liberated Fe2+ is subsequently 86 taken up from the soil solution by IRON-REGULATED TRANSPORTER1 (IRT1; Eide et al; 87 1996; Vert et al., 2002). The FRO2-mediated reduction of ferric Fe is supported by proton 88 extrusion via the P-type H+-ATPase AHA2 (Santi and Schmidt, 2009), a process that creates a 89 slightly acidic pH milieu in the apoplast that is favorable for the uptake of Fe. FRO2-mediated 90 ferric reduction is compromised at circumneutral or alkaline pH ranges (Susín et al., 1996; 4 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.16.431461; this version posted February 17, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 91 Lucena et al., 2007; Waters et al., 2018), rendering the strategy I system ineffective under such 92 conditions. 93 In Arabidopsis, the genes mediating the Fe deficiency responses are under the control of 94 a heterodimer consisting of the master transcription factor FER-LIKE IRON DEFICIENCY 95 INDUCED TRANSCRIPTION FACTOR (FIT; bHLH29) and one out of four clade Ib bHLH 96 proteins (bHLH38, bHLH39, bHLH100, and bHLH101) (Colangelo and Guerinot, 2004; Yuan et 97 al., 2008; Sivitz et al., 2012; Wang et al., 2013). The expression of FIT, in turn, and that of all Ib 98 bHLH proteins, is controlled by UPSTREAM REGULATOR OF IRT1 (URI/bHLH121) in 99 concert with IAA-LEUCINE RESISTANT3 (ILR3/bHLH105) (Kim et al., 2019; Gao et al., 100 2020a; Lei et al., 2020). FIT is negatively regulated by the clade IVa bHLH transcription factors 101 bHLH18, bHLH19, bHLH20, and bHLH25 (Cui et al., 2018), and by the E3 ligases BTSL1 and 102 BTSL2 (Rodríguez-Celma et al., 2019), which target FIT to degradation via the 26S proteasome. 103 A novel family of peptides designated IRONMAN/FE-UPTAKE-INDUCING PEPTIDE 104 (IMA/FEP), control Fe uptake presumably via regulation of clade Ib bHLH proteins (Grillet et 105 al., 2018; Hirayama et al., 2018). IMA/FEP peptides are ubiquitous across land plants and share 106 a highly conserved consensus motif at the C-terminus, which is necessary and sufficient for IMA 107 function. The expression of IMA1 and IMA2 is directly controlled by bHLH121 (Gao et al., 108 2020a). Ectopic expression of IMA1 overrides the repression of Fe uptake by sufficient internal 109 Fe levels, induces ferric reduction activity, and improves growth on media with low Fe solubility 110 (Grillet et al., 2018).
Load more

Recommended publications
  • Scopoletin 8-Hydroxylase: a Novel Enzyme Involved in Coumarin
    bioRxiv preprint doi: https://doi.org/10.1101/197806; this version posted October 4, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron- 2 deficiency responses in Arabidopsis 3 4 Running title: At3g12900 encodes a scopoletin 8-hydroxylase 5 6 Joanna Siwinska1, Kinga Wcisla1, Alexandre Olry2,3, Jeremy Grosjean2,3, Alain Hehn2,3, 7 Frederic Bourgaud2,3, Andrew A. Meharg4, Manus Carey4, Ewa Lojkowska1, Anna 8 Ihnatowicz1,* 9 10 1Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of 11 Gdansk, Abrahama 58, 80-307 Gdansk, Poland 12 2Université de Lorraine, UMR 1121 Laboratoire Agronomie et Environnement Nancy- 13 Colmar, 2 avenue de la forêt de Haye 54518 Vandœuvre-lès-Nancy, France; 3INRA, UMR 14 1121 Laboratoire Agronomie et Environnement Nancy-Colmar, 2 avenue de la forêt de Haye 15 54518 Vandœuvre-lès-Nancy, France; 16 4Institute for Global Food Security, Queen’s University Belfast, David Keir Building, Malone 17 Road, Belfast, UK; 18 19 [email protected] 20 [email protected] 21 [email protected] 22 [email protected] 23 [email protected] 24 [email protected] 25 [email protected] 26 [email protected] 27 [email protected] 28 *Correspondence: [email protected], +48 58 523 63 30 29 30 The date of submission: 02.10.2017 31 The number of figures: 9 (Fig.
    [Show full text]
  • Morning Glory Systemically Accumulates Scopoletin And
    Morning Glory Systemically Accumulates Scopoletin and Scopolin after Interaction with Fusarium oxysporum Bun-ichi Shimizua,b,*, Hisashi Miyagawaa, Tamio Uenoa, Kanzo Sakatab, Ken Watanabec, and Kei Ogawad a Graduate School of Agriculture, Kyoto University, Kyoto 606Ð8502, Japan b Institute for Chemical Research, Kyoto University, Uji 611-0011, Japan. Fax: +81-774-38-3229. E-mail: [email protected] c Ibaraki Agricultural Center, Ibaraki 311Ð4203, Japan d Kyushu National Agricultural Experiment Station, Kumamoto 861Ð1192, Japan * Author for correspondence and reprint requests Z. Naturforsch. 60c,83Ð90 (2005); received September 14/October 29, 2004 An isolate of non-pathogenic Fusarium, Fusarium oxysporum 101-2 (NPF), induces resis- tance in the cuttings of morning glory against Fusarium wilt caused by F. oxysporum f. sp. batatas O-17 (PF). The effect of NPF on phenylpropanoid metabolism in morning glory cuttings was studied. It was found that morning glory tissues responded to treatment with NPF bud-cell suspension (108 bud-cells/ml) with the activation of phenylalanine ammonia- lyase (PAL). PAL activity was induced faster and greater in the NPF-treated cuttings com- pared to cuttings of a distilled water control. High performance liquid chromatography analy- sis of the extract from tissues of morning glory cuttings after NPF treatment showed a quicker induction of scopoletin and scopolin synthesis than that seen in the control cuttings. PF also the induced synthesis of these compounds at 105 bud-cells/ml, but inhibited it at 108 bud- cells/ml. Possibly PF produced constituent(s) that elicited the inhibitory effect on induction of the resistance reaction.
    [Show full text]
  • Accumulation and Secretion of Coumarinolignans and Other Coumarins in Arabidopsis Thaliana Roots in Response to Iron Deficiency
    Accumulation and Secretion of Coumarinolignans and other Coumarins in Arabidopsis thaliana Roots in Response to Iron Deficiency at High pH Patricia Siso-Terraza, Adrian Luis-Villarroya, Pierre Fourcroy, Jean-Francois Briat, Anunciacion Abadia, Frederic Gaymard, Javier Abadia, Ana Alvarez-Fernandez To cite this version: Patricia Siso-Terraza, Adrian Luis-Villarroya, Pierre Fourcroy, Jean-Francois Briat, Anunciacion Aba- dia, et al.. Accumulation and Secretion of Coumarinolignans and other Coumarins in Arabidopsis thaliana Roots in Response to Iron Deficiency at High pH. Frontiers in Plant Science, Frontiers, 2016, 7, pp.1711. 10.3389/fpls.2016.01711. hal-01417731 HAL Id: hal-01417731 https://hal.archives-ouvertes.fr/hal-01417731 Submitted on 15 Dec 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. fpls-07-01711 November 21, 2016 Time: 15:23 # 1 ORIGINAL RESEARCH published: 23 November 2016 doi: 10.3389/fpls.2016.01711 Accumulation and Secretion of Coumarinolignans and other Coumarins in Arabidopsis thaliana Roots in Response to Iron Deficiency at
    [Show full text]
  • Isolated and Identified As Scopoletin (6-Methoxy-7-Hydroxycourmarin; 6-Methoxy- 7-Hydroxy-1,2-Benzopyrone)
    36 BOTANY: SKOOG AND MONTALDI PRoc. N. A. S. 22 Lake G. R., P. McCutchun, R. Van Meter, and J. C. Neel, Anal. Chem., 23, 1634 (1951). 23 Mackinney, G., J. Biol. Chem., 140, 315 (1941). 24 Hartree, B. E. F., in Modern Methods of Plant Analysis, ed. K. Paech, and M. V. Tracey (Berlin: Springer-Verlag, 1955), Vol. IV, p. 241. 25 Pharmacopeia of the United States, Fifteenth Revision (American Pharmaceutical Association, 1955), p. 186. 26 Ford, J. E., Brit. J. Nutrition, 7, 299 (1953). 27 Saltzman, B. E., Anal. Chem., 27, 284 (1955). " Galston, A. W., and S. M. Siegel, Science, 120, 1070 (1954). 29 Thimann, K. V., Amer. J. Bot., 43, 241 (1956). 30 Porter, J. W., Proc. Nutrition Soc. (Gr. Brit.), 12, 106 (1953). A UXIN-KINETIN INTERACTION REGULATING THE SCOPOLETIN AND SCOPOLIN LEVELS IN TOBACCO TISSUE CULTURES BY FOLKE SKOOG AND EDGARDO MONTALDI* DEPARTMENT OF BOTANY, UNIVERSITY OF WISCONSIN Communicated November 17, 1960 Tobacco tissue cultured in vitro has been observed to release a strongly ultra- violet fluorescent material into the agar medium. The intensity of the fluores- cence varies with the composition of the medium and the age and other conditions of the cultures. It is especially striking in the presence of high concentrations of 3- indoleacetic acid (IAA). A major fraction of the fluorescent material has been isolated and identified as scopoletin (6-methoxy-7-hydroxycourmarin; 6-methoxy- 7-hydroxy-1,2-benzopyrone). Influences of auxins and kinetin on its formation, its liberation from a glycosidic bound form, and its release into the medium will be reported.
    [Show full text]
  • PYK10 Myrosinase Reveals a Functional Coordination Between Endoplasmic Reticulum Bodies and Glucosinolates in Arabidopsis Thaliana
    The Plant Journal (2017) 89, 204–220 doi: 10.1111/tpj.13377 PYK10 myrosinase reveals a functional coordination between endoplasmic reticulum bodies and glucosinolates in Arabidopsis thaliana Ryohei T. Nakano1,2,3, Mariola Pislewska-Bednarek 4, Kenji Yamada5,†, Patrick P. Edger6,‡, Mado Miyahara3,§, Maki Kondo5, Christoph Bottcher€ 7,¶, Masashi Mori8, Mikio Nishimura5, Paul Schulze-Lefert1,2,*, Ikuko Hara-Nishimura3,*,#,k and Paweł Bednarek4,*,# 1Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, D-50829 Koln,€ Germany, 2Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, D-50829 Koln,€ Germany, 3Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan, 4Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland, 5Department of Cell Biology, National Institute of Basic Biology, Okazaki 444-8585, Japan, 6Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA, 7Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany, and 8Ishikawa Prefectural University, Nonoichi, Ishikawa 834-1213, Japan Received 29 March 2016; revised 30 August 2016; accepted 5 September 2016; published online 19 December 2016. *For correspondence (e-mails [email protected]; [email protected]; [email protected]). #These authors contributed equally to this work. †Present address: Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland. ‡Present address: Department of Horticulture, Michigan State University, East Lansing, MI, USA. §Present address: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan.
    [Show full text]
  • Accumulation and Secretion of Coumarinolignans and Other Coumarins in Arabidopsis Thaliana Roots in Response to Iron Deficiency at High Ph
    fpls-07-01711 November 21, 2016 Time: 15:23 # 1 ORIGINAL RESEARCH published: 23 November 2016 doi: 10.3389/fpls.2016.01711 Accumulation and Secretion of Coumarinolignans and other Coumarins in Arabidopsis thaliana Roots in Response to Iron Deficiency at High pH Patricia Sisó-Terraza1†, Adrián Luis-Villarroya1†, Pierre Fourcroy2‡, Jean-François Briat2, Anunciación Abadía1, Frédéric Gaymard2, Javier Abadía1 and Ana Álvarez-Fernández1* 1 Plant Stress Physiology Group, Department of Plant Nutrition, Aula Dei Experimental Station, Consejo Superior de Investigaciones Científicas, Zaragoza, Spain, 2 Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Edited by: Recherche Scientifique, Institut National de la Recherche Agronomique, Université Montpellier, Montpellier, France Janin Riedelsberger, University of Talca, Chile Root secretion of coumarin-phenolic type compounds has been recently shown to Reviewed by: Stefano Cesco, be related to Arabidopsis thaliana tolerance to Fe deficiency at high pH. Previous Free University of Bozen-Bolzano, Italy studies revealed the identity of a few simple coumarins occurring in roots and exudates Dierk Scheel, of Fe-deficient A. thaliana plants, and left open the possible existence of other Leibniz Institute of Plant Biochemistry, Germany unknown phenolics. We used HPLC-UV/VIS/ESI-MS(TOF), HPLC/ESI-MS(ion trap) and *Correspondence: HPLC/ESI-MS(Q-TOF) to characterize (identify and quantify) phenolic-type compounds Ana Álvarez-Fernández accumulated in roots or secreted into the nutrient solution of A. thaliana plants in [email protected] response to Fe deficiency. Plants grown with or without Fe and using nutrient solutions †These authors have contributed equally to this work. buffered at pH 5.5 or 7.5 enabled to identify an array of phenolics.
    [Show full text]
  • The Age of Coumarins in Plant–Microbe Interactions Pca Issue Special Ioannis A
    The Age of Coumarins in Plant–Microbe Interactions Special Issue Ioannis A. Stringlis *, Ronnie de Jonge and Corne´ M. J. Pieterse Plant-Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Padualaan 8, Utrecht, 3584 CH, The Netherlands *Corresponding author: E-mail, [email protected]; Fax,+31 30 253 2837. (Received February 9, 2019; Accepted April 23, 2019) Coumarins are a family of plant-derived secondary metab- For example, the cell wall-fortifying compounds lignin, cutin olites that are produced via the phenylpropanoid pathway. and suberin form structural barriers that inhibit pathogen in- In the past decade, coumarins have emerged as iron-mobi- vasion (Doblas et al. 2017). Other phenylpropanoid derivatives – Review lizing compounds that are secreted by plant roots and aid in such as flavonoids, anthocyanins and tannins participate in iron uptake from iron-deprived soils. Members of the cou- other aspects of environmental stress adaptation, or in plant marin family are found in many plant species. Besides their growth and physiology (Vogt 2010). More specifically, flavon- role in iron uptake, coumarins have been extensively studied oids emerged as important mediators of the chemical commu- for their potential to fight infections in both plants and nication between leguminous plants and beneficial nitrogen- animals. Coumarin activities range from antimicrobial and fixing rhizobia. In this mutualistic interaction, root-secreted fla- antiviral to anticoagulant and anticancer. In recent years, vonoids act as chemoattractants for rhizobia and activate genes studies in the model plant species tobacco and required for nodulation, which established the initial paradigm Arabidopsis have significantly increased our understanding for the role phenylpropanoid-derived metabolites in beneficial of coumarin biosynthesis, accumulation, secretion, chemical plant–microbe interactions (Fisher and Long 1992, Phillips modification and their modes of action against plant patho- 1992).
    [Show full text]
  • Identification of Qtls Affecting Scopolin and Scopoletin
    Identification of QTLs affecting scopolin and scopoletin biosynthesis in Arabidopsis thaliana Joanna Siwinska, Leszek Kadzinski, Rafal Banasiuk, Anna Gwizdek-Wisniewska, Alexandre Olry, Bogdan Banecki, Ewa Lojkowska, Anna Ihnatowicz To cite this version: Joanna Siwinska, Leszek Kadzinski, Rafal Banasiuk, Anna Gwizdek-Wisniewska, Alexandre Olry, et al.. Identification of QTLs affecting scopolin and scopoletin biosynthesis in Arabidopsis thaliana. BMC Plant Biology, BioMed Central, 2014, 14, 10.1186/s12870-014-0280-9. hal-01477746 HAL Id: hal-01477746 https://hal.univ-lorraine.fr/hal-01477746 Submitted on 10 May 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Siwinska et al. BMC Plant Biology 2014, 14:280 http://www.biomedcentral.com/1471-2229/14/280 RESEARCH ARTICLE Open Access Identification of QTLs affecting scopolin and scopoletin biosynthesis in Arabidopsis thaliana Joanna Siwinska1, Leszek Kadzinski1, Rafal Banasiuk1, Anna Gwizdek-Wisniewska1, Alexandre Olry2,3, Bogdan Banecki1, Ewa Lojkowska1 and Anna Ihnatowicz1* Abstract Background: Scopoletin and its glucoside scopolin are important secondary metabolites synthesized in plants as a defense mechanism against various environmental stresses. They belong to coumarins, a class of phytochemicals with significant biological activities that is widely used in medical application and cosmetics industry.
    [Show full text]
  • Two Glucosides of Coumarin Derivatives in Sweet Potato Roots Infected by Ceratocystis Fimbriata by Takao MINAMIKAWA, TAKASHI
    [Agr. Biol. Chem., Vol. 28, No. 4, p. 230•`233, 1964] Two Glucosides of Coumarin Derivatives in Sweet Potato Roots Infected by Ceratocystis Fimbriata By Takao MINAMIKAWA,TAKASHI AKAZAWA and IKUZO URITANI Laboratoryof BiochemistryNagoya University, Anjo, Aichi ReceivedJanuary 13, 1964 Two glucosides of coumarin derivatives were separated from sweet potato roots with black rot, by the combination of silica gel-coated thin layer chromatography and paper chromatography, and identified with skimmin and scopolin. The magnitude of synthesis of both bound coumarins was lower than that of the corresponding free coumarins, whereas they were detectable in neither cut nor fresh root tissues. sues7). During the course of this work, we INTRODUCTION also tried to find out whether or not the Several workers have reported the produc fungus infection would induce the produc tion of coumarins in plant tissues infected by tion of the bound forms of the respective pathogenic microorganisms1•`4) Post-infec coumarins in the root tissues. Hughes and tion synthesis of umbelliferone (7-hydroxy- Swain4) reported that about 25-fold increase coumarin) and scopoletin (6-methoxy-7-hy of scopolin (scopoletin-7-ƒÀ-glucoside) was in droxycoumarin) in sweet potato roots attack duced by the infection of Phytophthora in ed by the black rot fungus, Ceratocystis festans in potato tubers, whereas the chloro fimbriata, was reported by Uritani and Hoshi genic acid increase was only 2- to 5-fold in - ya5), and recently the third coumarin com the infected region. pound, esculetin (6,7-dihydroxycoumarin), This paper describes the chromatographic has been added to the list6). We have also identification of skimmin (umbelliferone-7-ƒÀ- carried out an analytical study on the synthe sis of the former two coumarins in the in glucoside) and scopolin in the sweet potato tissue with black rot.
    [Show full text]
  • Reference Substances 2018/2019
    Reference Substances 2018 / 2019 Reference Substances Reference 2018/2019 Contents | 3 Contents Page Welcome 4 Our Services 5 Reference Substances 6 Index I: Alphabetical List of Reference Substances and Synonyms 156 Index II: Plant-specific Marker Compounds 176 Index III: CAS Registry Numbers 214 Index IV: Substance Classification 224 Our Reference Substance Team 234 Order Information 237 Order Form 238 Prices insert 4 | Welcome Welcome to our new 2018 / 2019 catalogue! PhytoLab proudly presents the new you will also be able to view exemplary Index I contains an alphabetical list of all 2018 / 2019 catalogue of phyproof® certificates of analysis and download substances and their synonyms. It pro- Reference Substances. The seventh edition material safety data sheets (MSDS). vides information which name of a refer- of our catalogue now contains well over ence substance is used in this catalogue 1300 phytochemicals. As part of our We very much hope that our product and guides you directly to the correct mission to be your leading supplier of portfolio meets your expectations. The list page. herbal reference substances PhytoLab of substances will be expanded even has characterized them as primary further in the future, based upon current If you are a planning to analyse a specific reference substances and will supply regulatory requirements and new scientific plant please look for the botanical them together with the comprehensive developments. The most recent information name in Index II. It will inform you about certificates of analysis you are familiar will always be available on our web site. common marker compounds for this herb. with.
    [Show full text]
  • Min 2 3 4 5 6 7 Fluoresce N Ce Mau 1 2 3 4 5 6 7 Esculin Scopolin Fraxin
    1 2 3 4 5 6 7 Fluorescence mAu Fluorescence Esculin Fraxin Scopolin 2 3 4 5 6 7 min Figure S1 : Inhibition of esculetin uptake by orthovanadate addition. Representative fluorescence chromatograms obtained using λexc 365 and λem 460 nm for root extracts of Arabidopsis f6’h1 mutant seedlings grown for 7 days on Hoagland agar plates and then transferred 7 days on Hoagland agar containing poorly 3- available Fe and supplemented with 50µM esculetin and different concentrations of orthovanadate (VO4 ). 3- 3- 3- 3- 3- 1:+0µMVO4 ,2:+50µMVO4 ,3:+100µMVO4 ,4:+200µMVO4 ,5:+250µMVO4 , 6: + 500 3- 3- µM VO4 ,7:+1mMVO4 . 90 1200 80 1000 70 60 800 50 600 40 30 400 Fraxin (nmol/g) 20 Scopolin (nmol/g) * 200 10 0 0 +Fe +Fe +Glyb +Fe +Fe +Glyb Figure S2. Inhibition of fraxetin uptake by the ATP-dependent transport inhibitor glibenclamide. Uptake of coumarins by roots grown in hydroponic Hoagland solution containing poorly available Fe (+Fe), supplemented or not with 150 µM glibenclamide (Glyb). Plants were kept for three days in the presence of coumarins and glibenclamide and coumarin glucosides were analyzed by HPLC. t test significant difference: *P <0.05(n = 4 biological repeats). Bars represent means ± SD. Intens. Fraxetin + Fe pH8 : ‐MS x106 467.9896 4 2 207.0359 676.0282 936.9805 0 100 200 300 400 500 600 700 800 900 m/z Intens. Fraxetin + Fe pH7 : ‐MS x106 4 467.9893 2 207.0359 331.9027 936.9799 0 100 200 300 400 500 600 700 800 900 m/z Intens.
    [Show full text]
  • Reference Substances
    Reference Substances 2020/2021 Contents | 3 Contents Page Welcome 4 Our Services 5 Reference Substances 6 Index I: Alphabetical List of Reference Substances and Synonyms 168 Index II: CAS Registry Numbers 190 Index III: Substance Classification 200 Our Reference Substance Team 212 Distributors & Area Representatives 213 Ordering Information 216 Order Form 226 4 | Welcome Welcome to our new 2020 / 2021 catalogue! PhytoLab proudly presents the new for all reference substances are available Index I contains an alphabetical list of 2020/2021 catalogue of phyproof® for download. all substances and their synonyms. It Reference Substances. The eighth edition provides information which name of a of our catalogue now contains well over We very much hope that our product reference substance is used in this 1400 natural products. As part of our portfolio meets your expectations. The catalogue and guides you directly to mission to be your leading supplier of list of substances will be expanded even the correct page. herbal reference substances PhytoLab further in the future, based upon current has characterized them as primary regulatory requirements and new Index II contains a list of the CAS registry reference substances and will supply scientific developments. The most recent numbers for each reference substance. them together with the comprehensive information will always be available on certificates of analysis you are familiar our web site. However, if our product list Finally, in Index III we have sorted all with. does not include the substance you are reference substances by structure based looking for please do not hesitate to get on the class of natural compounds that Our phyproof® Reference Substances will in touch with us.
    [Show full text]