Roles of Neddylation Against Viral Infections

LETTER TO THE EDITOR

Roles of neddylation against viral infections

Kun Han and Jiyan Zhang

Cellular & Molecular Immunology (2018) 15, 292–294; doi:10.1038/cmi.2017.100; published online 27 November 2017

he ubiquitin-dependent proteasome lentivirus by SAMHD1,7 or Rift Valley is carried out by certain CRLs members. Tpathway can be hijacked by certain fever virus by protein kinase R.8 Both Therefore, we speculated that neddyla- viruses to maintain viral genome ampli- CRLs-dependent and CRLs-independent tion should promote the HSV-1-induced fication. A key class of ubiquitin-E3s mechanisms have been suggested in this production of type I interferons, and this involved in this pathway is Cullin- regard.9 hypothesis was confirmed in our study, RING ligases (CRLs), which are activated As for the innate immune response, which was recently published in Cellular by an additional ubiquitin-like protein the involvement of neddylation was sug- & Molecular Immunology (Figure 1).14 NEDD8.1 The process by which the gested as early as the year 2006.10 The With primary macrophages deficient of ubiquitin-like protein NEDD8 is conju- transcription of type I interferon genes is the catalytic subunit of neddylation E1, gated to its target proteins is officially controlled by nuclear factor-κB (NF-κB) we have provided the first evidence that named ‘neddylation’. Consequently, ned- and interferon regulatory factor (IRF) neddylation indeed plays a role in type I dylation inhibition, through the use family members including IRF3. Many interferon production. Interestingly, ned- of the pharmacological inhibitor viruses execute immune-evasive activities dylation blockade only delays but does MLN4924, might prevent viral genome by targeting the type I interferon signal- not completely abrogate HSV-1-induced amplification.2,3 Herpesviruses, adeno- ing pathway. RNA viruses such as Sendi p65 nuclear translocation.14 Conse- virus, and influenza virus are MLN4924 virus (SeV), vesicular stomatitis virus, quently, the early phase type I interferon susceptible, whereas vaccinia virus and and rotavirus induce the degradation of production in HSV-1 infection is much – vesicular stomatitis virus are not.2,3 Ned- IRF3.10 12 However, a herpesvirus family more impaired than the late phase.14 dylation might also promote viral repli- member, herpes simplex virus type I Recently, the notion that the host also cation independent of CRLs.4 For (HSV-1), a DNA virus, has no such exploits the neddylation pathway to fight example, hepatitis B virus-encoded X effect.13 Even though the degradation of against virus infection has been sup- protein can act as an NEDD8 substrate IRF3 upon infection with rotavirus has ported by additional evidence. Zhang T and its neddylation by E3 ligase HDM2 been demonstrated to be independent of et al. reported that the polymerase basic enhances its stability and function in viral CRLs,11,12 a Cullin-based ubiquitin ligase protein 2 of influenza A virus can be replication and hepatocarcinogenesis.4 On pathway was reported to be involved in covalently modified by NEDD8, which the other hand, blockade of neddylation SeV-induced IRF3 degradation in 2006.10 reduces its stability and blocks the repli- prevents the degradation of host restric- Therefore, neddylation promotes IRF3 cation of influenza A virus.15 tion factors and thereby restores the degradation upon SeV infection.10 The- Another group later reported that restriction of human immunodeficiency oretically, neddylation should promote NEDD8 knockdown showed no effect virus by APOBEC3G and SAMHD1,5,6 IRF3-mediated transcription of type I on type I interferon production triggered interferon genes. by lipopolysaccharide (LPS), poly (I:C), Department of Molecular Immunology, Institute of In addition to IRF family members, or SeV.16 Since poly (I:C) and SeV Basic Medical Sciences, 27 Taiping Road, Beij- NF-κB also plays a key role in mediating induce IRF3 degradation, whereas ing 100850, China 10 Correspondence: Dr J Zhang, PhD, MD, the transcription of type I interferon HSV-1 does not, perhaps the interplay Department of Molecular Immunology, Institute genes. It is demonstrated that neddyla- between the NF-κB pathway and IRF of Basic Medical Sciences, 27 Taiping Road, tion contributes to NF-κB activity by pathway determines the outcome. Intri- Beijing, PA 100850, China fi E-mail: [email protected] promoting the ubiquitination and sub- guingly, MLN4924 signi cantly inhibited Received: 2 August 2017; Accepted: 14 August sequent degradation of IκBproteins type I interferon production triggered by 2017 since the ubiquitination of IκBproteins LPS, poly (I:C), or SeV,16 which has been Neddylation in viral infection K Han and J Zhang

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Figure 1 Neddylation contributes to DNA virus-induced production of type I interferons. The transcription of type I interferon genes is controlled by nuclear factor-κB (NF-κB) and interferon regulatory factor (IRF) family members IRF3 and IRF7. DNA virus-induced NF-κB activation depends on the ubiquitination and subsequent degradation of IκB proteins. The ubiquitination of IκBproteinssuchasIκBα is carried out by a certain member of the Cullin-RING ligases (CRLs), that is, SKP1/Cullin-1/F-box protein β-TrCP/Roc1 complex. As CRLs are activated by the covalent conjugation of an additional ubiquitin-like protein NEDD8 to Cullins, neddylation promotes DNA virus-induced NF-κB activation and thereby promotes DNA virus-induced production of type I interferons. attributed by the authors to its off-target SAMHD1-mediated restriction of HIV-1. – effects. However, the potential off-target 1 Enchev RI, Schulman BA, Peter M. Protein J Virol 2013; 87: 11741 11750. neddylation: beyond cullin-RING ligases. Nat 7 Wei W, Guo H, Liu X, Zhang H, Qian L, Luo K effects of small interfering RNAs and Rev Mol Cell Biol 2015; 16:30–44. et al. A first-in-class NAE inhibitor, the relatively low efficiency of small 2 Hughes DJ, Wood JJ, Jackson BR, Baquero- MLN4924, blocks lentiviral infection in mye- loid cells by disrupting neddylation- RNA interference cannot be excluded. Perez B, Whitehouse A. NEDDylation is essential for Kaposi's sarcoma-associated dependent Vpx-mediated SAMHD1 degrada- Future studies are required to address herpesvirus latency and lytic reactivation tion. JVirol2014; 88:745–751. this issue. and represents a novel anti-KSHV target. 8 Mudhasani R, Tran JP, Retterer C, Kota KP, Whitehouse CA, Bavari S. Protein kinase R Together, these 10 years of studies Plos Pathog 2015; 11: e1004771. 3 Le-Trilling VT, Megger DA, Katschinski B, degradation is essential for rift valley fever suggest that neddylation might exert Landsberg CD, Rückborn MU, Tao S et al. virus infection and is regulated by SKP1- distinct roles in the infection of different Broad and potent antiviral activity of the NAE CUL1-F-box (SCF)FBXW11-NSs E3 ligase. inhibitor MLN4924. Sci Rep 2016; 6: Plos Pathog 2016; 12: e1005437. viruses. Targeting the neddylation 19977. 9 Ramirez PW, DePaula-Silva AB, Szaniawski M, pathway against virus infection, though 4 Liu N, Zhang J, Yang X, Jiao T, Zhao X, Li W Barker E, Bosque A, Planelles V. HIV-1 Vpu hopeful under certain circumstances, et al. HDM2 promotes NEDDylation of HBV utilizes both cullin-RING ligase (CRL) HBx to enhance its stability and function. dependent and independent mechanisms should be cast with caution and JVirol2017; 91:e00340–17. to downmodulate host proteins. Retrovirol- requires extensive studies to clarify how 5 Stanley DJ, Bartholomeeusen K, Crosby DC, ogy 2015; 12: 65. neddylation works in each step of virus Kim DY, Kwon E, Yen L et al. Inhibition of a 10 Bibeau-Poirier A, Gravel SP, Clément JF, NEDD8 cascade restores restriction of HIV by Rolland S, Rodier G, Coulombe P et al. infection. APOBEC3G. Plos Pathog 2012; 8: Involvement of the IkappaB kinase (IKK)- e1003085. related kinases tank-binding kinase 1/IKKi 6 Hofmann H, Norton TD, Schultz ML, Polsky SB, and cullin-based ubiquitin ligases in IFN CONFLICT OF INTEREST Sunseri N, Landau NR. Inhibition of CUL4A regulatory factor-3 degradation. JImmunol The authors declare no conflict of interest. Neddylation causes a reversible block to 2006; 177:5059–5067.

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11 Ding S, Mooney N, Li B, Kelly MR, Feng N, 13 Collins SE, Noyce RS, Mossman KL. Innate 15 Zhang T, Ye Z, Yang X, Qin Y, Hu Y, Tong X Loktev AV et al. Comparative proteomics cellular response to virus particle entry et al. NEDDylation of PB2 reduces its reveals strain-specific beta-TrCP degradation requires IRF3 but not virus replication. stability and blocks the replication of via rotavirus NSP1 hijacking a host Cullin-3- J Virol 2004; 78:1706–1717. influenza A virus. Sci Rep 2017; 7: Rbx1 complex. Plos Pathog 2016; 12: 14 Zhang X, Ye Z, Pei Y, Qiu G, Wang Q, 43691. e1005929. Xu Y et al. Neddylation is required for 16 Song H, Huai W, Yu Z, Wang W, Zhao J, 12 Lutz LM, Pace CR, Arnold MM. Rotavirus herpes simplex virus type I (HSV-1)- Zhang L et al. MLN4924, a first-in-class NSP1 associates with components of the induced early phase interferon-beta pro- NEDD8-activating enzyme inhibitor, attenu- Cullin RING ligase family of E3 ubiquitin duction. Cell Mol Immunol 2016; 13: ates IFN-beta production. JImmunol2016; ligases. JVirol2016; 90:6036–6048. 578–583. 196:3117–3123.

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