Recombinant Human ADH6 Protein Catalog Number: ATGP1564

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Recombinant human ADH6 protein Catalog Number: ATGP1564 PRODUCT INPORMATION Expression system E.coli Domain 1-375aa UniProt No. P28332 NCBI Accession No. NP_001095940 Alternative Names Alcohol dehydrogenase 6, ADH-5 PRODUCT SPECIFICATION Molecular Weight 42.4 kDa (399aa) confirmed by MALDI-TOF Concentration 0.5mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT Purity > 90% by SDS-PAGE Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description ADH6, also known as alcohol dehydrogenase 6, is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' uTR, which is a steroid hormone receptor binding site. Recombinant human ADH6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. 1 Recombinant human ADH6 protein Catalog Number: ATGP1564 Amino acid Sequence MGSSHHHHHH SSGLVPRGSH MGSHMSTTGQ VIRCKAAILW KPGAPFSIEE VEVAPPKAKE VRIKVVATGL CGTEMKVLGS KHLDLLYPTI LGHEGAGIVE SIGEGVSTVK PGDKVITLFL PQCGECTSCL NSEGNFCIQF KQSKTQLMSD GTSRFTCKGK SIYHFGNTST FCEYTVIKEI SVAKIDAVAP LEKVCLISCG FSTGFGAAIN TAKVTPGSTC AVFGLGGVGL SVVMGCKAAG AARIIGVDVN KEKFKKAQEL GATECLNPQD LKKPIQEVLF DMTDAGIDFC FEAIGNLDVL AAALASCNES YGVCVVVGVL PASVQLKISG QLFFSGRSLK GSVFGGWKSR QHIPKLVADY MAEKLNLDPL ITHTLNLDKI NEAVELMKTG KCIRCILLL General References Yasunami M., et al. (1991) Proc. Natl. Acad. Sci. u.S.A. 88:7610-7614 Hillier L.W., et al. (2005) Nature. 434:724-731 DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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