Microvesicles in Stored

Louise Knauth Grifols Australia Microvesicles (MV)/

What are they ?!? • Traditional categorization is submicron particles which are between 0.1 to 1µm in diameter. • MV can be derived from different haematological and non‐ haematological lines ‐ , , erythrocytes and epithelial cells. • MV are formed when a membrane vesicle is released by an activated parent cell. Mechanisms for cell vesiculation vary. • Importantly, the MV reflect the composition of the parent cell. • Every one has them in low levels. Stored Red Cells

• Storage lesion. In vivo aging different to in vitro aging. • After 50 days of storage, around x20 increase in MV. More pronounced between 60 and 120 days. • Haemolysis in stored blood is indicated by the concentration of extracellular haemoglobin, 50% of which may be attributed to haemoglobin‐containing ErMVs. • Filtration removes platelets and a significant number of and red cell microparticles. Depends on filter. • Aging red cells = increased PS = procoagulant. Is this why up to 30% cells are ‘lost’ once transfused? Stored Red Cells

• Erythrocyte‐derived MV can promote by initiating thrombin generation independent of . • Studies show that transfusion of older red cells carries a higher risk of DVT and multi‐organ failure. • Platelet activation may activate and bind white cells present in non‐ leucoreduced packed cells > and procoagulant state. • ErMV have also shown a balancing anti‐coagulant effect by prolonging the consolidation of fibrinogen to fibrin, especially if the red cells are washed. Stored Plasma

• Plasma derived from whole blood or apheresis collection. • Processing of plasma for transfusion may induce MV. – Filter for leucodepletion may remove MV, but also reduce clotting factors and fibrinogen. – Mixed findings coagulation results – Recommend leucodepletion within 10 hours of collecting whole blood. Thank you References

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