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Production of Biosurfactant Via Locally Isolated Actinomycete Fermentation..Pdf Project Code : (for RCMO use only) RU GRANT UNIVERSITI SAINS MALAYSIA FINAL REPORT FORM Please email a softcopy of this report to [email protected] A PROJECT DETAILS Title of Research: PRODUCTION OF BIOSURFACTANT VIA LOCALLY ISOLATED ACTINOMYCETE FERMENTATION ii Account Number: 1001/PBIOLOGI/815103 iii Name of Research Leader: ASSOCIATE PROFESSOR AHMAD RAMLI MOHO YAHYA iv Name of Co-Researcher: 1 .. ASSOCIATE PROFESSOR LATIFFAH ZAKARIA v Duration of this research: a) Start Date 15/12/2012 ~ b) Completion Date 14/06/2015 c) Duration 30 MONTHS d) Revised Date (if any) 8 ABSTRACT OF RESEARCH (An abstract of between 100 and 200 words must be prepared in Bahasa Malaysia and in English. This abstract will be included in the Report of the Research and Innovation Section at a later date as a means of presenting the project findings of the researcherls to the University and the community at large) Kajian ini memberi tumpuan kepada penyaringan dan penilaian penghasilan biosurfaktan ekstrasel daripada 33 pencilan actinomycetes. Daripada 33 pencilan, pencilan R1, dicirikan sebagai genus Streptomyces berdasarkan analisis fenotip and genotip, telah dipilih sebagai model organisma berfilamen dalam fermentasi aerobic untuk penghasilan biosurfaktan. Penghasilan biosurfaktan telah disiasat dalam mod kelompok menggunakan kelalang goncang dan 3 L bioreactor tangki teraduk. Penghasilan biosurfaktan maksimum dalam kelalang goncang didapati pada jam 144 dalam medium yang mengandungi 6% (v/v) minyak sawit, 0.6% (w/v) ekstrak yis, 2% (v/v) Tween® 80, 0. 7% (w/v) NaCI dan pH awal 6 dengan 4% (v/v) kepekatan inokulum. Penambahbaikan penghasilan biosurfaktan telah dica ai den an men unakan reaktor tan ki teraduk an dikawal den an baik. Penin katan RU Grant Final Report I RCMO July 2015 1 sebanyak 3 kali ganda aktiviti pengemulsian dan ukuran ketegangan permukaan terendah (40.5±0.05 dynes/em) telah dicapai apabila kultur diaduk pada 600 rpm. Dalam kultur kelompok, penghasilan biosurfaktan tidak begitu bergantung kepada biojisim tetapi dipengaruhi oleh morfologi kultur semas.a fermentasi tenggelam. Kaedah pengekstrakan pelarut dengan menggunakan methanol, chloroform and 1-butanol (1: 1:1) memberi hasil ekstrak biosurfaktan mentah tertinggi (58.04 g/L) yang dicirikan sebagai biosurfaktan glikolipid. ,., The present study focused on screening and evaluating extracellular biosurfactant production from 33 actinomycete isolates. Out of 33 isolates, isolate R1, characterized as the genus Streptomyces based on phenotypic and genotypic analyses, was selected as a model filamentous organism in an aerobic fermentation for biosurfactant production. The production of biosurfactant was investigated in batch mode using shake flasks and 3 L stirred tank bioreactor. The maximum biosurfactant production in shake flasks was obtained at 144 hours in a medium containing 6% (v/v) palm oil, 0.6% (w/v) yeast extract, 2% (v/v) Tween 80, 0.7% (w/v) NaCI and initial pH 6 with 4% (v/v) inoculum concentration. Further improvement of biosurfactant production was achieved using a well-controlled stirred tank reactor. An increase of 3-fold of emulsification activity and lowest surface tension measurement (40.5±0.05 dynes/em) was attained when the culture was agitated at 600 rpm. In batch cultures, the production of biosurfactant was not dependent on biomass as much but rather was influenced by culture morphology during submerged fermentation. Solvent extraction method using methanol, chloroform and 1-butanol (1: 1:1) gave the highest yield (58.04 g/L) of crude biosurfactant extract that was characterized as glycolipid biosurfactant. C BUDGET & EXPENDITURE Total Approved Budget : RM RM 243,934.00 Yearly Budget Distributed Year 1 : RM 117,967.00 Year 2 : RM 125,967.00 Year 3 : RM - Total Expenditure : RM 243,073.25 Balance : RM 890.75 Percentage of Amount Spent(%) : 99.64% # Please attach final account statement (eStatement) to indicate the project expenditure ii Equipment Purchased Under Vot 35000 No. Name of Equipment Amount (RM) Location Status # Please attach the Asset/Inventory Return Form (Borang Penyerahan Asetllnventori)- Appendix 1 RU Grant Final Report I RCMO July 2015 2 D RESEARCH ACHIEVEMENTS Project Objectives (as stated/approved in the project proposal) i No. Project Objectives Achievement To screen potential biosurfactant-producing actinomycetes 1 Achieved from the local environment Identification and characterization of the potential 2 Achieved biosurfactant-producer . To establish good growth conditions of biosurfactant- 3 Achieved producing actinomycete , To enhance the production of biosurfactant via batch 4 Achieved I fermentation 5 6 ii Research Output a) Publications in lSI Web of Science/Scopus Status of Publication Publication No. (published/accepted/ (authors,title,journal,year,volume,pages,etc.) under review) Nor Syafirah Zambry, Adilah Ayoib, Nur Asshifa Md Noh, and Ahmad Ramli Mohd Yahya. Production and partial , characterization of biosurfactant produced by Streptomyces Published sp. R 1. Bioprocess and Biosystems Engineering (20 17) (ISSN 1615-7591) b) Publications in Other Journals Status of Publication Publication No. (published/accepted/ (authors, title,journal, year, volume, pages,etc.) under review) 1 Nurfarah Aina Mohamed Razalli, Norazurin Syuhada Rusly, Siti Zulaikha Mohd Yusof, Mohd Syafiq Awang, Nor Syafirah Zamry, Shahkillahwati Mohd Ridhwan, Nur Asshifa Md Noh Published , and Ahmad R.M. Yahya, Microbial Biosurfactants,Australian Biotechnology, The Journal of AusBiotech (2016), 26(3), 40-41 c) Other Publications (book,chapters in book,monograph,magazine,etc.) Status of Publication Publication No. (published/accepted/ (authors, title,journal, year, volume, pages,etc.) under review) RU Grant Final Report I RCMO July 2015 3 d) Conference Proceeding Conference Level No. Title of Abstract/Article " (conference name,date, place) (International/National) 1 1st International Conference Screening of Biosurfactant ~ On Sustainable Agriculture Producers from Locally Isolated International and Environment Actinobacterial 2 The Inaugural Asian Effect of Impeller Tip Speed on Conference on the Life Biosurfactant Production by International Sciences & Sustainabilitv Streptomyces sp. R1 3 The Inaugural Asian Isolation and Identification of Conference on the Life Actinobacteriai-Biosurfactant International Sciences & Sustainability Producers # Please attach a full copy of the publication/proceeding listed above iii Other Research Ouput/lmpact From This Project (patent, products, awards, copyright, external grant, networking, etc.) Product : Collection of actinobacterial isolates producing biosurfactants E HUMAN CAPITAL DEVELOPMENT a) Graduated Human Capital Nationality (No.) Student Name National International 1. PhD 2. 1. Nor Syafirah Zambry MSc 2 2. Adilah Ayoib 1. Norazurin Syuhada Rusly 2. lza Syazwani Mardzuan Undergraduate 5 3. Mok Kar Mun 4. Nur Asna Azhar 5. Jonathan Tan Choon Siang b) On-going Human Capital Nationality (No.) Student Name National International 1 1 .. PhD 2. 1. MSc 2. 1. Undergraduate 2. c) Others Human Capital Nationality (No.) Student Name National International 1. Post Doctoral Fellow 2. RU Grant Final Report I RCMO July 2015 4 1. Nor Syafirah Zambry * Research Officer 3 2. Nur Asshifa Md Noh 3. Shahkillawati Mohd Ridhwan 1. Nik Farhana Nadiah Nik Mustapha 2. Adilah Ayoib* Research Assistant 5 3. lntan Sakinah Mohd Anuar 4. Mohd Syafiq Awang 5. Siti Zulaikha Mohd Yusof 1. Others(. .................... ) 2. F COMPREHENSIVE TECHNICAL REPORT Applicants are required to prepare a comprehensive technical report explaining the project. The following format should be used (this report must be attached separately): • Introduction • Objectives • Methods • Results • Discussion • Conclusion and Suggestion • Acknowledgements • References G PROBLEMS/CONSTRAINTS/CHALLENGES IF ANY (Please provide issues arising from the project and how they were resolved) H RECOMMENDATION (Please provide recommendations that can be used to improve the delivery of information, grant management, guidelines and policy, etc.) Project Leader's Signature: Name : ASSOC. PROF. AHMAD RAMLI MOHO. YAHYA SCHOOL OF BIOLOGICAL SCIENCES 0 t . , J:NIV~RS I SAINS MALAYSIA a e . ~I c::?r1 Of~PENANG RU Grant Final Report I RC 0 July 2015 5 COMMENTS, IF ANY/ENDORSEMENT BY PTJ'S RESEARCH ~ONIMITrji~ , .................~.~-~······.•··································································································· Signature and Stamp of Chairperson of PT J's Evaluation Committee Name: PROFESOR MADYA DR. YAHVA 81N MAT ARIP Tlmbalan Oekan Date : Penyelldlkan. Slswa~ah & Jarlngan Pusat Pengajlan Salns Kajihayat Universiti Sains Malaysia ..._. ... ~ .... :........ PRO::s,:: ::n::?.~~i~:::::~~H S1gn ure and mp o lfttl Director of PT J Uniw~rsiti Sains Malaysia Name: Date : RU Grant Final Report I RCMO July 2015 6 ' •. FINAL TECHNICAL REPORT PRODUCTION OF BIOSURFACTANTS VIA LOCALLY ISOLATED ACTINOMYCETE FERMENTATION RESEARCH UNIVERSITY GRANT (RUI) 1001/PBIOLOGI/8151 03 15/12/2012- 14/06/2015 RM243,934.00 AHMAD RAMLI MOHD. YAHYA, PhD. School of Biological Sciences f Final Report: Production ofBiosurfactants via Locally-Isolated Actinomycete Fermentation ' ' RUJ 1001/PBJOLOGJ/815103 Contents 1.0 INTRODUCTION ............................................................................................................... 1 2.0 OBJECTIVES ...................................................................................................................... 1 3.0 RESEARCH METHODOLOGY ........................................................................................
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