New Records of Streptomyces and Non Streptomyces Actinomycetes Isolated from Soils Surrounding Sana'a High Mountain
International Journal of Research in Pharmacy and Biosciences Volume 3, Issue 3, March 2016, PP 19-31 ISSN 2394-5885 (Print) & ISSN 2394-5893 (Online) New Records of Streptomyces and Non Streptomyces Actinomycetes Isolated from Soils Surrounding Sana'a High Mountain Qais Yusuf M. Abdullah1, Maher Ali. Al-Maqtari2, Ola, A A. Al-Awadhi3 Abdullah Y. Al-Mahdi 4 Department of Biology, Microbiology Section Faculty of Science, Sana'a University1, 3, 4 Department of Chemistry, Biochemistry Section, Faculty of Science, Sana'a University2 ABSTRACT Actinomycetes are ubiquitous soil-dwelling saprophytes known to produce secondary metabolites may of which are antibiotic. 20 soil samples were collected from three different sites at high altitude environments surrounding Sana'a city, which ranged from 2300-3000 m above sea level as a prime source of promising native rare actinomycetes. 516 actinomycetes isolates were isolated in pure culture and five selective pretreatment isolation methods. Out of 232 isolates, 26 actinomycetes showing good activity. The identification of 26 selected actinomycetes based on cultural morphology, physiology and biochemical characterization. From the preceding bacteria, thirteen actinomycetes were recorded for the first time from Yemeni soil of these: four non-Streptomyces namely (Intrasporangium sp., Nocardiodes luteus, Sporichthya polymorpha and Streptovirticillium cinnamoneum) and nine Streptomyces namely (S. anulatus, S. celluloflavus, S. cellulosae, S. chromofucus, S. erythrogriseus, S. flavidvirens, S. flavissimus, S. globosus and S. griseoflavus). The results of this study suggested that the soil of high mountains such as Sana'a Mountains could be an interesting source to explore new strains that recorded for the first time in Yemen, Sana'a City.
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