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Digital Method Development Principles, Benefits, and Potential Pitfalls March 2018 Volume 31 Number 3 www.chromatographyonline.com Digital Method Development Principles, benefits, and potential pitfalls LC TROUBLESHOOTING GC CONNECTIONS BIOPHARMACEUTICAL Eluent preparation for HILIC The past and present of GC PERSPECTIVES detection Peptide mapping using micro-pillar array columns All other trademarks are the property of their respective owners. WHOSE TRIPLE QUAD CAN GIVE YOU 15% MORE TIME? Copyright © 2016 PerkinElmer, Inc. 400358A_03. All rights reserved. PerkinElmer® is a registered trademark of SIMPLE: PERKINELMER. Whether you’re performing environmental, food, or industrial testing, your lab needs to analyze more and increasingly complex samples under tighter regulatory standards. Because our new QSight™ triple-quad LC/MS/MS needs no routine cleaning and maintenance, you get up to 15% more uptime (that’s up to 35 more days per year) to take on your increased testing workload. Plus, our patented technology delivers exceptional sensitivity and reliability along with all that productivity. QSight: A triple quad that simply knows the value of time. Learn more at www.perkinelmer.com/QSight Put Our Insight to Work For You Imagine how productive you could be if you had access to a global team of Real Stories from the Lab experts who strive to deliver insight in every interaction with your lab. With True Story 44: Agilent CrossLab, that’s exactly what you get. Lab-Wide Data Analytics Consider us for compliance services, complete lab relocations, lab analytics System data insight leads to to optimize performance, on-site and on-demand training, as well as complete improved lab performance. service coverage for your Agilent and non-Agilent instruments. We will also See this story and more at consult with you to develop methods and recommend the best columns and agilent.com/chem/story44 supplies to optimize your results. Find out more at www.agilent.com/crosslab © Agilent Technologies, Inc. 2018 March | 2018 Volume 31 Number 3 COVER STORY 120 A Practical Approach to Modelling of Reversed-Phase Liquid Chromatographic Separations:arations: Advantages,Advantages, Principles, and Possible Pitfalls Patrik Petersson, Bernard O. Boateng, Jennifer K. Field, and Melvin R. Euerby Chromatographic principles and best practices for obtaining highly precise retention time, peak width, and resolution predictions for the optimization ofof reversed-phase LC separations using commercially available retention modelling software will be discussed. Columns 144 LC TROUBLESHOOTING Eluent Preparation for Hydrophilic Interaction Liquid Chromatography, Part 2: pH, Buffers, and Gradient Elution Dwight R. Stoll and Claudia Seidl When preparing buffers for HILIC separations, is the buffer concentration considered relative to the aqueous portion of the eluent only, or the aqueous/organic mixture? 150 GC CONNECTIONS A Compendium of GC Detection, Past and Present John V. Hinshaw This instalment reviews GC detectors, both past and vanished, as well as current and relevant to today’s separation challenges. 155 BIOPHARMACEUTICAL PERSPECTIVES Peptide Mapping of Monoclonal Antibodies and Antibody–Drug Conjugates Using Micro-Pillar Array Columns Combined with Mass Spectrometry Koen Sandra, Jonathan Vandenbussche, Isabel Vandenheede, Bo Claerebout, Jeff Op de Beeck, Paul Jacobs, Wim De Malsche, Gert VISIT US! • Hall B2 • Stand 128 Desmet, and Pat Sandra This article reports on the use of micro-pillar array columns for peptide mapping of monoclonal antibodies and antibody–drug conjugates. Departments 167 Products Editorial Policy: 169 The Applications Book All articles submitted to LC•GC Europe 178 Events are subject to a peer-review process in association with the magazine’s Editorial Advisory Board. Cover: Original materials courtesy: echo3005/ Shutterstock.com 116 LC•GC Europe March 2018 Delivering Smart Solutions The GCMS-QP2020 and the GCMS Insight software package dramatically improve the efficiency of daily analysis procedures The Smart SIM creates optimal methods automatically and enables a batch analysis of more than 400 components, thereby reducing the number of measurements. Simultaneous analysis of 434 pesticides using Smart SIM With LabSolutions Insight software, quantita- tive results for a complete series of data files can be displayed side-by-side, enabling easy identification of any outliers, which shortens analysis time. www.shimadzu.eu/gcms-qp2020 Published by UBM Americas Editorial Advisory Board Daniel W. 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