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(12) United States Patent (10) Patent No.: US 9,506,915 B2 Tackett Et Al USOO9506915B2 (12) United States Patent (10) Patent No.: US 9,506,915 B2 Tackett et al. (45) Date of Patent: *Nov. 29, 2016 (54) METHODS AND KITS FOR ISOLATION AND 2012fO270273 A1 10/2012 Zhang et al. ANALYSIS OF A CHROMATIN REGION 2015,0111788 A1* 4/2015 Fernandez ......... C12N 15, 1093 506/26 (71) Applicants: Alan J. Tackett, Little Rock, AR (US); Stephanie Byrum, Little Rock, AR OTHER PUBLICATIONS (US); Sean Taverna, Little Rock, AR Agelopoulos et al., “Developmental regulation of chromatin con (US); Kevin Raney, Little Rock, AR formation by Hox proteins in Drosophila'. Cell Rep., 2012, pp. (US); Zachary Waldrip, Little Rock, 350-359, vol. 1, No. 4. AR (US) Akiyoshi et al., “Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit”. Genes & Devel (72) Inventors: Alan J. Tackett, Little Rock, AR (US); opment, 2009, pp. 2887-2899, vol. 23. 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(Continued) (56) References Cited Primary Examiner — Jacob Cheu (74) Attorney, Agent, or Firm — Polsinelli PC U.S. PATENT DOCUMENTS (57) ABSTRACT 6,453,242 B1 9/2002 Eisenberg et al. The present invention encompasses methods of identifying 6,534,261 B1 3/2003 Cox, III et al. proteins and protein modifications of proteins specifically 2011 O239315 A1 9/2011 Bonas et al. associated with a chromatin. 2011 O262908 A1 10/2011 Kingston et al. 2012/0040857 A1 2/2012 Kingston et al. 16 Claims, 22 Drawing Sheets 2012/0110685 A1 5/2012 Bonas et al. (12 of 22 Drawing Sheet(s) Filed in Color) US 9,506,915 B2 Page 2 (56) References Cited Platt et al., “The yeast galactose genetic Switch is mediated by the formation of a Gal4p-Ga180p-Gal3p complex”. The EMBO Jour nal, 1998, pp. 4086-4091, vol. 17, No. 14. OTHER PUBLICATIONS Pokholok et al., “Genome-wide Map of Nucleosome Acetylation Fu et al., “High frequency off-target mutagenesis induced by and Methylation in Yeast”. 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