DOCSLIB.ORG

Structure and Function of the Toscana Virus Cap-Snatching Endonuclease

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Structure and Function of the Toscana Virus Cap-Snatching Endonuclease Structure and function of the Toscana virus cap-snatching endonuclease Rhian Jones, Sana Lessoued, Kristina Meier, Stéphanie Devignot, Sergio Barata-García, Maria Mate, Gabriel Bragagnolo, Friedemann Weber, Maria Rosenthal, Juan Reguera To cite this version: Rhian Jones, Sana Lessoued, Kristina Meier, Stéphanie Devignot, Sergio Barata-García, et al.. Struc- ture and function of the Toscana virus cap-snatching endonuclease. Nucleic Acids Research, Oxford University Press, 2019, 47, pp.10914-10930. 10.1093/nar/gkz838. hal-02611184 HAL Id: hal-02611184 https://hal-amu.archives-ouvertes.fr/hal-02611184 Submitted on 18 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial| 4.0 International License 10914–10930 Nucleic Acids Research, 2019, Vol. 47, No. 20 Published online 4 October 2019 doi: 10.1093/nar/gkz838 Structure and function of the Toscana virus cap-snatching endonuclease Rhian Jones1,†, Sana Lessoued1,†, Kristina Meier3,Stephanie´ Devignot4, Sergio Barata-Garc´ıa1, Maria Mate1, Gabriel Bragagnolo1, Friedemann Weber 4, Maria Rosenthal 3 and Juan Reguera 1,2,* 1Aix-Marseille Universite,´ CNRS, AFMB UMR 7257, 13288 Marseille, France, 2INSERM, AFMB UMR7257,13288 Marseille, France, 3Bernhard Nocht Institute for Tropical Medicine, Department of Virology, D-20359 Hamburg, Downloaded from https://academic.oup.com/nar/article-abstract/47/20/10914/5580917 by guest on 18 May 2020 Germany and 4Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, D-35392 Giessen, Germany Received March 11, 2019; Revised September 14, 2019; Editorial Decision September 17, 2019; Accepted October 01, 2019 ABSTRACT INTRODUCTION Toscana virus (TOSV) is an arthropod-borne hu- Toscana virus (TOSV) is a member of the genus phle- man pathogen responsible for seasonal outbreaks of bovirus (family Phenuiviridae,orderBunyavirales), that in- fever and meningoencephalitis in the Mediterranean cludes several arthropod-borne human pathogens such as basin. TOSV is a segmented negative-strand RNA Sandfly fever sicilian virus (SFSV), Severe fever with throm- virus (sNSV) that belongs to the genus phlebovirus bocytopenia syndrome virus (SFTSV) or Rift Valley fever virus (RVFV), that cause outbreaks in the Mediterranean (family Phenuiviridae, order Bunyavirales), encom- basin, Asia, and sub-Saharan Africa, respectively. Symp- passing other important human pathogens such as toms range from mild to severe central nervous system in- Rift Valley fever virus (RVFV). Here, we carried out fections (TOSV, SFSV) up to hemorrhagic fevers (SFTSV, a structural and functional characterization of the RV F V ) ( 1–3). The absence of efficient drugs and vaccines TOSV cap-snatching endonuclease, an N terminal for these emerging pathogens means that there is an urgent domain of the viral polymerase (L protein) that pro- need for research and development of tools for their diag- vides capped 3OH primers for transcription. We re- nostics and treatment. port TOSV endonuclease crystal structures in the Like all segmented negative-strand RNA viruses apo form, in complex with a di-ketoacid inhibitor (sNSVs), Phenuiviridae family members use a ‘cap- (DPBA) and in an intermediate state of inhibitor re- snatching’ mechanism to initiate transcription of the viral lease, showing details on substrate binding and ac- genome. By this mechanism, host cell messenger RNAs (mRNAs) are sequestered and cleaved by the EN around 11 tive site dynamics. The structure reveals substan- nucleotides downstream of the capped 5 end. The resulting tial folding rearrangements absent in previously re- small capped RNA fragments are then used for the initia- ported cap-snatching endonucleases. These include tion of viral transcription by the L protein RNA-dependent the relocation of the N terminus and the appear- RNA polymerase (RdRp) domain. Cap-snatching has been ance of new structural motifs important for transcrip- extensively structurally and biochemically characterized tion and replication. The enzyme shows high activ- for influenza virus, which replicates in the cell nucleus and ity rates comparable to other His+ cap-snatching en- has a heterotrimeric polymerase instead of the monomeric donucleases. Moreover, the activity is dependent on L protein present in bunyaviruses (4). The comparison of conserved residues involved in metal ion and sub- the structures of influenza A and B polymerases illustrated strate binding. Altogether, these results bring new the possible mechanics underlying this process; whereby light on the structure and function of cap-snatching the cap binding domain (in the PB2 subunit), captures the cellular mRNA and orientates it towards the EN domain endonucleases and pave the way for the develop- (at the N terminus of the PA subunit) for its cleavage ment of specific and broad-spectrum antivirals. before re-directing the RNA into the RdRp catalytic site (in the PB1 subunit) for priming transcription initiation (5–7). Bunyavirales carry out cap-snatching by their mul- tifunctional L protein, which harbours the EN at the N *To whom correspondence should be addressed. Tel: +33 491 825 582; Fax: +33 491 266 720; Email: [email protected] †The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors. C The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Research, 2019, Vol. 47, No. 20 10915 terminus. There are no L protein EN structures available fluenza virus, the enzyme lacks essential residues of the cat- for any Phenuiviridae family members, and certain lines alytic motif and consequently lacks EN activity. This sug- of evidence suggest that the cap-snatching processes may gests that THOV has possibly evolved an alternative, and differ among the sNSVs. Firstly, considering the similar still elusive, strategy to gain access to the cap structure re- architecture of sNSV polymerases reported to date (8), quired for their mRNAs (26). for arenaviruses the length of the host-derived 5 mRNA Besides the conserved residues defining their active sites, fragments (5–6 nucleotides (9–11)) is too short to allow the cap-snatching ENs of the different sNSV families have transcription priming via the mechanism proposed for no apparent sequence similarity (25). This prevents trans- influenza virus (requiring fragments of 10 to 15 nucleotides fer of the available structural information between families. (12)). This is even more remarkable for the influenza-related Consequently, there is a gap in our knowledge regarding Thogotoviruses (THOV; family Orthomyxoviridae), that structure-to-function relationships of cap-snatching ENs contains only one heterologous nucleotide in the 5 end of for many important human pathogens. This is the case for Downloaded from https://academic.oup.com/nar/article-abstract/47/20/10914/5580917 by guest on 18 May 2020 their mRNAs (13,14). Additionally, the sources of mRNA all members of the Nairoviridae and Phenuiviridae families in the nucleus (where influenza virus replicates and tran- of the order Bunyavirales. Here, we present the crystal struc- scribes) are different from those in the cytoplasm (where ture of the Phenuiviridae member TOSV cap-snatching EN bunyaviruses replicate and transcribe). In conclusion, in the apo form and in complex with the di-ketoacid in- structural and/or mechanistic variations in the influenza hibitor 2,4-dioxo-4-phenylbutanoic acid (DPBA). We char- virus cap-snatching mechanism occur among sNSVs and acterize its enzymatic activity, specificity and the impor- still need to be understood. tance of each catalytic residue in the reaction. We identify The presence of a cap-snatching EN is a common fea- new residues involved in substrate binding and investigate ture among the sNSV polymerases. The first structural and their role in catalysis. The structure reveals new features ex- functional characterization of a cap-snatching EN was re- clusive to Phenuiviridae family that we show have functional ported for influenza virus (15,16). These structures revealed implications beyond transcription. We discuss the implica- that the EN belongs to the metal ion dependent PD-D/ExK tions of these new structural features for the cap-snatching nuclease superfamily, including many different enzymes in- mechanism in the context of the full-length L proteins and volved in various aspects of DNA metabolism (e.g. EcoRV). for the evolution of the super large L proteins found in The motif includes acidic residues responsible for metal tenuiviruses and nairoviruses. ion coordination, and a lysine responsible for the stabilisa- tion and orientation of the attacking water hydroxide nu- MATERIALS AND METHODS
Load more

Recommended publications
  • Generic Amplification and Next Generation Sequencing Reveal
    Dinçer et al. Parasites & Vectors (2017) 10:335 DOI 10.1186/s13071-017-2279-1 RESEARCH Open Access Generic amplification and next generation sequencing reveal Crimean-Congo hemorrhagic fever virus AP92-like strain and distinct tick phleboviruses in Anatolia, Turkey Ender Dinçer1†, Annika Brinkmann2†, Olcay Hekimoğlu3, Sabri Hacıoğlu4, Katalin Földes4, Zeynep Karapınar5, Pelin Fatoş Polat6, Bekir Oğuz5, Özlem Orunç Kılınç7, Peter Hagedorn2, Nurdan Özer3, Aykut Özkul4, Andreas Nitsche2 and Koray Ergünay2,8* Abstract Background: Ticks are involved with the transmission of several viruses with significant health impact. As incidences of tick-borne viral infections are rising, several novel and divergent tick- associated viruses have recently been documented to exist and circulate worldwide. This study was performed as a cross-sectional screening for all major tick-borne viruses in several regions in Turkey. Next generation sequencing (NGS) was employed for virus genome characterization. Ticks were collected at 43 locations in 14 provinces across the Aegean, Thrace, Mediterranean, Black Sea, central, southern and eastern regions of Anatolia during 2014–2016. Following morphological identification, ticks were pooled and analysed via generic nucleic acid amplification of the viruses belonging to the genera Flavivirus, Nairovirus and Phlebovirus of the families Flaviviridae and Bunyaviridae, followed by sequencing and NGS in selected specimens. Results: A total of 814 specimens, comprising 13 tick species, were collected and evaluated in 187 pools. Nairovirus and phlebovirus assays were positive in 6 (3.2%) and 48 (25.6%) pools. All nairovirus sequences were closely-related to the Crimean-Congo hemorrhagic fever virus (CCHFV) strain AP92 and formed a phylogenetically distinct cluster among related strains.
    [Show full text]
  • 2020 Taxonomic Update for Phylum Negarnaviricota (Riboviria: Orthornavirae), Including the Large Orders Bunyavirales and Mononegavirales
    Archives of Virology https://doi.org/10.1007/s00705-020-04731-2 VIROLOGY DIVISION NEWS 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales Jens H. Kuhn1 · Scott Adkins2 · Daniela Alioto3 · Sergey V. Alkhovsky4 · Gaya K. Amarasinghe5 · Simon J. Anthony6,7 · Tatjana Avšič‑Županc8 · María A. Ayllón9,10 · Justin Bahl11 · Anne Balkema‑Buschmann12 · Matthew J. Ballinger13 · Tomáš Bartonička14 · Christopher Basler15 · Sina Bavari16 · Martin Beer17 · Dennis A. Bente18 · Éric Bergeron19 · Brian H. Bird20 · Carol Blair21 · Kim R. Blasdell22 · Steven B. Bradfute23 · Rachel Breyta24 · Thomas Briese25 · Paul A. Brown26 · Ursula J. Buchholz27 · Michael J. Buchmeier28 · Alexander Bukreyev18,29 · Felicity Burt30 · Nihal Buzkan31 · Charles H. Calisher32 · Mengji Cao33,34 · Inmaculada Casas35 · John Chamberlain36 · Kartik Chandran37 · Rémi N. Charrel38 · Biao Chen39 · Michela Chiumenti40 · Il‑Ryong Choi41 · J. Christopher S. Clegg42 · Ian Crozier43 · John V. da Graça44 · Elena Dal Bó45 · Alberto M. R. Dávila46 · Juan Carlos de la Torre47 · Xavier de Lamballerie38 · Rik L. de Swart48 · Patrick L. Di Bello49 · Nicholas Di Paola50 · Francesco Di Serio40 · Ralf G. Dietzgen51 · Michele Digiaro52 · Valerian V. Dolja53 · Olga Dolnik54 · Michael A. Drebot55 · Jan Felix Drexler56 · Ralf Dürrwald57 · Lucie Dufkova58 · William G. Dundon59 · W. Paul Duprex60 · John M. Dye50 · Andrew J. Easton61 · Hideki Ebihara62 · Toufc Elbeaino63 · Koray Ergünay64 · Jorlan Fernandes195 · Anthony R. Fooks65 · Pierre B. H. Formenty66 · Leonie F. Forth17 · Ron A. M. Fouchier48 · Juliana Freitas‑Astúa67 · Selma Gago‑Zachert68,69 · George Fú Gāo70 · María Laura García71 · Adolfo García‑Sastre72 · Aura R. Garrison50 · Aiah Gbakima73 · Tracey Goldstein74 · Jean‑Paul J. Gonzalez75,76 · Anthony Grifths77 · Martin H. Groschup12 · Stephan Günther78 · Alexandro Guterres195 · Roy A.
    [Show full text]
  • Baseline Mapping of Severe Fever with Thrombocytopenia Syndrome Virology, Epidemiology and Vaccine Research and Development ✉ Nathen E
    www.nature.com/npjvaccines REVIEW ARTICLE OPEN Baseline mapping of severe fever with thrombocytopenia syndrome virology, epidemiology and vaccine research and development ✉ Nathen E. Bopp1,8, Jaclyn A. Kaiser2,8, Ashley E. Strother1,8, Alan D. T. Barrett1,2,3,4 , David W. C. Beasley 2,3,4,5, Virginia Benassi 6, Gregg N. Milligan2,3,4,7, Marie-Pierre Preziosi6 and Lisa M. Reece 3,4 Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emergent tick-borne bunyavirus first discovered in 2009 in China. SFTSV is a growing public health problem that may become more prominent owing to multiple competent tick-vectors and the expansion of human populations in areas where the vectors are found. Although tick-vectors of SFTSV are found in a wide geographic area, SFTS cases have only been reported from China, South Korea, Vietnam, and Japan. Patients with SFTS often present with high fever, leukopenia, and thrombocytopenia, and in some cases, symptoms can progress to severe outcomes, including hemorrhagic disease. Reported SFTSV case fatality rates range from ~5 to >30% depending on the region surveyed, with more severe disease reported in older individuals. Currently, treatment options for this viral infection remain mostly supportive as there are no licensed vaccines available and research is in the discovery stage. Animal models for SFTSV appear to recapitulate many facets of human disease, although none of the models mirror all clinical manifestations. There are insufficient data available on basic immunologic responses, the immune correlate(s) of protection, and the determinants of severe disease by SFTSV and 1234567890():,; related viruses.
    [Show full text]
  • Supporting Information
    Supporting Information Rosenberg et al. 10.1073/pnas.1307243110 SI Results and Discussion domestic ungulates (horses, cows, sheep, goats, camels, and pigs) Of the 83 arboviruses, nonhuman vertebrate hosts have been and rodents in both groups might be a consequence of spatial identified for 70 (84%); the remaining 13 are presumed to be proximity to humans. Sentinel monkeys were often used in pro- zoonoses because there is no indication they can be transmitted cedures to isolate arboviruses, which might account for their directly between humans by vectors (Table S1). Animal hosts have higher representation among arboviruses. In contrast, there are been identified for at least 57 (44%) of the 130 nonarboviruses; an few published records of bats being routinely sampled during additional 5 (8%) are presumed on epidemiological evidence to arbovirus studies, and only two arboviruses (3%) have been iso- have nonhuman reservoirs (Table S1). A number of viruses infect lated from bats. The reason a much larger number of arbovirus more than one nonhuman vertebrate host species and it is likely species (n = 16) have been isolated from birds than have that the variety of hosts is wider than has been recorded. The nonarbovirus species (n = 1) might, however, be characteristic of predominant host groups for arboviruses (n = 70) are nonhuman the pathogenicity of the togaviruses and flaviviruses, which are primates (31%), rodents (29%), ungulates (26%), and birds (23%); much more common among the arboviruses. The most prominent for the nonarboviruses (n = 57), they are rodents (30%), ungu- vectors of arboviruses were mosquitoes (67%), ticks (19%), and lates (26%), bats (23%), and primates (16%).
    [Show full text]
  • Arboviruses of Human Health Significance in Kenya Atoni E1,2
    Arboviruses of Human Health significance in Kenya Atoni E1,2#, Waruhiu C1,2#, Nganga S1,2, Xia H1, Yuan Z1 1. Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing, 100049, China #Authors contributed equally to this work Correspondence: Zhiming Yuan, [email protected]; Tel.: +86-2787198195 Summary In tropical and developing countries, arboviruses cause emerging and reemerging infectious diseases. The East African region has experienced several outbreaks of Rift valley fever virus, Dengue virus, Chikungunya virus and Yellow fever virus. In Kenya, data from serological studies and mosquito isolation studies have shown a wide distribution of arboviruses throughout the country, implying the potential risk of these viruses to local public health. However, current estimates on circulating arboviruses in the country are largely underestimated due to lack of continuous and reliable countrywide surveillance and reporting systems on arboviruses and disease vectors and the lack of proper clinical screening methods and modern facilities. In this review, we discuss arboviruses of human health importance in Kenya by outlining the arboviruses that have caused outbreaks in the country, alongside those that have only been detected from various serological studies performed. Based on our analysis, at the end we provide workable technical and policy-wise recommendations for management of arboviruses and arboviral vectors in Kenya. [Afr J Health Sci. 2018; 31(1):121-141] and mortality. Recently, the outbreak of Zika virus Introduction became a global public security event, due to its Arthropod-borne viruses (Arboviruses) are a cause ability to cause congenital brain abnormalities, of significant human and animal diseases worldwide.
    [Show full text]
  • Tick Cell Lines for Study of Crimean-Congo Hemorrhagic Fever
    Edinburgh Research Explorer Tick Cell Lines for Study of Crimean-Congo Hemorrhagic Fever Virus and Other Arboviruses Citation for published version: Sakyi, L, Kohl, A, Bente, DA & Fazakerley, JK 2012, 'Tick Cell Lines for Study of Crimean-Congo Hemorrhagic Fever Virus and Other Arboviruses', Vector-Borne and Zoonotic Diseases, vol. 12, no. 9, pp. 769-781. https://doi.org/10.1089/vbz.2011.0766 Digital Object Identifier (DOI): 10.1089/vbz.2011.0766 Link: Link to publication record in Edinburgh Research Explorer Document Version: Peer reviewed version Published In: Vector-Borne and Zoonotic Diseases General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Download date: 26. Sep. 2021 7/9/13 Tick Cell Lines for Study of Crimean-Congo Hemorrhagic Fever Virus and Other Arboviruses VECTOR BORNE AND ZOONOTIC DISEASES Vector Borne Zoonotic Dis. 2012 September; 12(9): 769–781. PMCID: PMC3438810 doi: 10.1089/vbz.2011.0766 Tick Cell Lines for Study of Crimean-Congo Hemorrhagic Fever Virus and Other Arboviruses Lesley Bell-Sakyi, 1,* Alain Kohl,1,* Dennis A.
    [Show full text]
  • Diversity and Evolutionary Origin of the Virus Family Bunyaviridae
    Diversity and Evolutionary Origin of the Virus Family Bunyaviridae Dissertation zur Erlangung des Doktorgrades (Dr. rer. nat.) der Mathematisch-Naturwissenschaftlichen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn vorgelegt von Marco Marklewitz aus Hannover Bonn, 2016 Angefertigt mit Genehmigung der Mathematisch-Naturwissenschaftlichen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn 1. Gutachter: Prof. Dr. Christian Drosten 2. Gutachter: Prof. Dr. Bernhard Misof Tag der Promotion: 21.12.2016 Erscheinungsjahr: 2017 Danksagung Zu Beginn möchte ich mich ganz herzlich bei meinem Doktorvater Prof. Christian Drosten bedanken, dass er mir ermöglicht hat, an einem solch vielfältigen und spannenden Thema zu arbeiten. Für Fragen hatte er jederzeit ein offenes Ohr und bei auftretenden Problemen war er immer sehr hilfsbereit. Des Weiteren möchte ich mich herzlich bei meiner Prüfungskommission, bestehend aus meinem 2. Gutachter Prof. Bernhard Misof sowie Prof. Clemens Simmer und PD Dr. Lars Podsiadlowski, für ihre Zeit und Bereitschaft danken mich zu prüfen. Mein ganz besonder Dank geht an PD Dr. Sandra Junglen für ihre hervorragende und kompetente Betreuung während der Jahre meiner Doktorarbeit. Ich habe es als eine Ehre empfunden, ein Teil ihrer zu Beginn noch sehr jungen Arbeitsgruppe zu sein, danke ihr sehr für ihr Vertrauen und hoffe, sie mit meiner (zukünftigen) Arbeit stolz zu machen. Die Atmosphäre in ihrer Arbeitsgruppe ist immer sehr positiv und ermöglicht, die Arbeit mit viel Spaß zu verbinden. Insbesondere möchte ich herausstellen, dass ich ihr für die Möglichkeit besonders dankbar bin, neben meiner Doktorarbeit Feldarbeiten in Panama durchzuführen. Diese Zeit hat mein Leben auf die positivste Art und Weise nachhaltig beeinflusst. Mein großer Dank gilt auch allen Kolleginnen und Kollegen in den Virologie-Laboratorien der Augenklinik für ihre ständige Hilfs- und Diskussionsbereitschaft, ihr Zuhören bei Problemen sowie für ihre Freundschaft über all die Jahre.
    [Show full text]
  • The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus Title Phlebovirus
    The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus Title Phlebovirus Matsuno, Keita; Kajihara, Masahiro; Nakao, Ryo; Nao, Naganori; Mori-Kajihara, Akina; Muramatsu, Mieko; Qiu, Author(s) Yongjin; Torii, Shiho; Igarashi, Manabu; Kasajima, Nodoka; Mizuma, Keita; Yoshii, Kentaro; Sawa, Hirofumi; Sugimoto, Chihiro; Takada, Ayato; Ebihara, Hideki mSphere, 3(3), e00239-18 Citation https://doi.org/10.1128/mSphere.00239-18 Issue Date 2018-06-13 Doc URL http://hdl.handle.net/2115/71358 Rights(URL) https://creativecommons.org/licenses/by/4.0/ Type article File Information e00239-18.full.pdf Instructions for use Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP RESEARCH ARTICLE Ecological and Evolutionary Science crossm The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus Phlebovirus Keita Matsuno,a,b Masahiro Kajihara,c Ryo Nakao,d Naganori Nao,e Akina Mori-Kajihara,c Mieko Muramatsu,c Yongjin Qiu,f Shiho Torii,g Manabu Igarashi,b,c Nodoka Kasajima,c Keita Mizuma,a Kentaro Yoshii,h Hirofumi Sawa,b,g Chihiro Sugimoto,b,i Downloaded from Ayato Takada,b,c Hideki Ebiharaj aLaboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan bDivision of International Services, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan cDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan
    [Show full text]
  • Electron Microscopy in Discovery of Novel and Emerging Viruses from the Collection of the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA)
    viruses Review Electron Microscopy in Discovery of Novel and Emerging Viruses from the Collection of the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) Vsevolod L. Popov 1,2,3,4,5,*, Robert B. Tesh 1,2,3,4,5, Scott C. Weaver 2,3,4,5,6 and Nikos Vasilakis 1,2,3,4,5,* 1 Department of Pathology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555, USA; [email protected] 2 Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555, USA; [email protected] 3 Institute for Human Infection and Immunity, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555, USA 4 Center for Tropical Diseases, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555, USA 5 World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555, USA 6 Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555, USA * Correspondence: [email protected] (V.L.P.); [email protected] (N.V.); Tel.: +1-409-747-2423 (V.L.P.); +1-409-747-0650 (N.V.) Received: 30 April 2019; Accepted: 24 May 2019; Published: 25 May 2019 Abstract: Since the beginning of modern virology in the 1950s, transmission electron microscopy (TEM) has been an important and widely used technique for discovery, identification and characterization of new viruses. Using TEM, viruses can be differentiated by their ultrastructure: shape, size, intracellular location and for some viruses, by the ultrastructural cytopathic effects and/or specific structures forming in the host cell during their replication.
    [Show full text]
  • 2021 Taxonomic Update of Phylum Negarnaviricota (Riboviria: Orthornavirae), Including the Large Orders Bunyavirales and Mononegavirales
    Archives of Virology https://doi.org/10.1007/s00705-021-05143-6 VIROLOGY DIVISION NEWS 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales Jens H. Kuhn1 · Scott Adkins2 · Bernard R. Agwanda211,212 · Rim Al Kubrusli3 · Sergey V. Alkhovsky (Aльxoвcкий Cepгeй Bлaдимиpoвич)4 · Gaya K. Amarasinghe5 · Tatjana Avšič‑Županc6 · María A. Ayllón7,197 · Justin Bahl8 · Anne Balkema‑Buschmann9 · Matthew J. Ballinger10 · Christopher F. Basler11 · Sina Bavari12 · Martin Beer13 · Nicolas Bejerman14 · Andrew J. Bennett15 · Dennis A. Bente16 · Éric Bergeron17 · Brian H. Bird18 · Carol D. Blair19 · Kim R. Blasdell20 · Dag‑Ragnar Blystad21 · Jamie Bojko22,198 · Wayne B. Borth23 · Steven Bradfute24 · Rachel Breyta25,199 · Thomas Briese26 · Paul A. Brown27 · Judith K. Brown28 · Ursula J. Buchholz29 · Michael J. Buchmeier30 · Alexander Bukreyev31 · Felicity Burt32 · Carmen Büttner3 · Charles H. Calisher33 · Mengji Cao (曹孟籍)34 · Inmaculada Casas35 · Kartik Chandran36 · Rémi N. Charrel37 · Qi Cheng38 · Yuya Chiaki (千秋祐也)39 · Marco Chiapello40 · Il‑Ryong Choi41 · Marina Ciufo40 · J. Christopher S. Clegg42 · Ian Crozier43 · Elena Dal Bó44 · Juan Carlos de la Torre45 · Xavier de Lamballerie37 · Rik L. de Swart46 · Humberto Debat47,200 · Nolwenn M. Dheilly48 · Emiliano Di Cicco49 · Nicholas Di Paola50 · Francesco Di Serio51 · Ralf G. Dietzgen52 · Michele Digiaro53 · Olga Dolnik54 · Michael A. Drebot55 · J. Felix Drexler56 · William G. Dundon57 · W. Paul Duprex58 · Ralf Dürrwald59 · John M. Dye50 · Andrew J. Easton60 · Hideki Ebihara (海老原秀喜)61 · Toufc Elbeaino62 · Koray Ergünay63 · Hugh W. Ferguson213 · Anthony R. Fooks64 · Marco Forgia65 · Pierre B. H. Formenty66 · Jana Fránová67 · Juliana Freitas‑Astúa68 · Jingjing Fu (付晶晶)69 · Stephanie Fürl70 · Selma Gago‑Zachert71 · George Fú Gāo (高福)214 · María Laura García72 · Adolfo García‑Sastre73 · Aura R.
    [Show full text]
  • Detection and Phylogenetic Analysis of Phlebovirus, Including Severe Fever with Thrombocytopenia Syndrome Virus, in Ticks Collected from Tokyo, Japan
    NOTE Virology Detection and phylogenetic analysis of phlebovirus, including severe fever with thrombocytopenia syndrome virus, in ticks collected from Tokyo, Japan Nami MATSUMOTO1), Hiroaki MASUOKA1), Kazuhiro HIRAYAMA1), Akio YAMADA1) and Kozue HOTTA1)* 1)Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan ABSTRACT. Severe fever with thrombocytopenia syndrome (SFTS) was detected for the first J. Vet. Med. Sci. time in China in 2011. Since then, human cases have been reported in endemic regions, including 80(4): 638–641, 2018 Japan. To investigate the presence of tick-borne pathogens in Tokyo, 551 ticks (266 samples) were collected from October 2015 to October 2016. Although the SFTS virus was not detected by doi: 10.1292/jvms.17-0604 RT-PCR, a novel phlebovirus was detected in one sample. In a phylogenetic analysis based on the partial nucleotide sequences of the L and S segments of the virus, the virus clustered with Lesvos virus (Greece), Yongjia tick virus, and Dabieshan tick virus (China). Further studies involving virus Received: 13 November 2017 isolation are required to characterize this novel phlebovirus and to expand the epidemiological Accepted: 28 January 2018 knowledge of related pathogens. Published online in J-STAGE: KEY WORDS: phlebovirus, tick, tick-borne pathogen, Tokyo 26 February 2018 The genus Phlebovirus, which includes the causative pathogen for severe fever with thrombocytopenia syndrome (SFTS), was classified in the order Bunyavirales, family Phenuviridae in the 10th Report of the International Committee for Taxonomy of Virus (ICTV) (https://talk.ictvonline.org/taxonomy/). SFTS is one of the most serious tick-borne diseases.
    [Show full text]
  • Enfermedades Virales Transmitidas Por Garrapatas
    Enfermedades virales transmitidas por garrapatas Katterine Molina-Hoyos1, Carolina Montoya-Ruiz1, Francisco J. Díaz2, Juan David Rodas1 RESUMEN Los virus transmitidos por garrapatas (VTG) pertenecen a las familias Flaviviridae, Bunyaviri- dae, Reoviridae, Asfarviridae y Orthomyxoviridae y son agentes causales de diferentes enfer- medades en humanos y animales. Debido a la creciente importancia epidemiológica que es- tán teniendo los VTG, esta revisión pretende englobar el conocimiento actual de estos agentes y las enfermedades que producen, así como exponer las estrategias abordadas en prevención y tratamiento que se han implementado hasta el momento en diferentes países. Es evidente que para la región Neotropical hacen falta estudios sobre los VTG presentes en la región, ya que la gran mayoría de los artículos, tanto revisiones de tema como trabajos originales, pre- sentan datos de las regiones Neártica y Paleártica. Considerando el panorama actual de los estudios de VTG en la región Neotropical y las particularidades de la misma, es muy probable que existan otros VTG aún no identificados que podrían tener algún impacto en salud pública. PALABRAS CLAVE Arbovirus; Enfermedades Transmitidas por Garrapatas; Latinoamérica; Salud Pública; Zoonosis SUMMARY Tick-borne viruses and their diseases Tick-borne viruses (TBVs) belong to the Flaviviridae, Bunyaviridae, Reoviridae, Asfarviridae and Orthomyxoviridae families and cause different diseases in humans and animals. Due to the epidemiologic relevance of TBVs, this review highlights the actual knowledge of these agents and the diseases they cause, besides of the prevention and treatment strategies implemented 1 Grupo de Investigación en Ciencias Veterinarias Centauro. Línea de zoonosis emergentes y re-emergentes. Facultad de Ciencias Agrarias, Universidad de Antioquia, Medellín, Colombia.
    [Show full text]