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LIMA1 Antibody Cat

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LIMA1 Antibody Cat LIMA1 Antibody Cat. No.: 8053 LIMA1 Antibody Immunohistochemistry of LIMA1 in human colon tissue Immunofluorescence of LIMA1 in human colon tissue with with LIMA1 antibody at 5 μg/mL. LIMA1 antibody at 20 μg/mL. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (89%), Pig: (72%) LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. IMMUNOGEN: The immunogen is located within amino acids 230 - 280 of LIMA1. TESTED APPLICATIONS: ELISA, IF, IHC-P, WB September 26, 2021 1 https://www.prosci-inc.com/lima1-antibody-8053.html LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this SPECIFICITY: antibody will only detect the three largest isoforms. POSITIVE CONTROL: 1) Cat. No. 1320 - Human Colon Tissue Lysate Predicted: 50, 66, 84 kDa PREDICTED MOLECULAR WEIGHT: Observed: 65 kDa Properties PURIFICATION: LIMA1 antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: LIMA1 antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL LIMA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. Additional Info OFFICIAL SYMBOL: LIMA1 ALTERNATE NAMES: LIM domain and actin-binding protein 1, PP624, EPLIN, SREBP3 ACCESSION NO.: NP_001107018 PROTEIN GI NO.: 165905589 GENE ID: 51474 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 26, 2021 2 https://www.prosci-inc.com/lima1-antibody-8053.html The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the BACKGROUND: cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4). 1) Maul RS and Chang DD. EPLIN, epithelial protein lost in neoplasm. Oncogene 1999; REFERENCES: 18:7838-41. 2) Maul RS, Song Y, Amann KJ, et al. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 2003; 160:399-407. 3) Abe K and Takeichi M. EPLIN mediates linkage of the cadherin catenin complex to F- actin and stabilizes the circumferential actin belt. Proc. Natl. Acad. Sci. USA 2008; 105:13-9. 4) Zhang S, Wang X, Iqval S, et al. Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition. J. Biol. Chem. 2013; 288:1469-79. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/lima1-antibody-8053.html.
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