EPLIN (LIMA1) (NM 016357) Human Tagged ORF Clone – RG211066

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EPLIN (LIMA1) (NM_016357) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: EPLIN (LIMA1) (NM_016357) Human Tagged ORF Clone Tag: TurboGFP Symbol: LIMA1 Synonyms: EPLIN; LDLCQ8; SREBP3 Vector: pCMV6-AC-GFP (PS100010) E. coli Selection: Ampicillin (100 ug/mL) Cell Selection: Neomycin

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ORF Nucleotide >RG211066 representing NM_016357 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGAATCATCTCCATTTAATAGACGGCAATGGACCTCACTATCATTGAGGGTAACAGCCAAAGAACTTT CTCTTGTCAACAAGAACAAGTCATCGGCTATTGTGGAAATATTCTCCAAGTACCAGAAAGCAGCTGAAGA AACAAACATGGAGAAGAAGAGAAGTAACACCGAAAATCTCTCCCAGCACTTTAGAAAGGGGACCCTGACT GTGTTAAAGAAGAAGTGGGAGAACCCAGGGCTGGGAGCAGAGTCTCACACAGACTCTCTACGGAACAGCA GCACTGAGATTAGGCACAGAGCAGACCATCCTCCTGCTGAAGTGACAAGCCACGCTGCTTCTGGAGCCAA AGCTGACCAAGAAGAACAAATCCACCCCAGATCTAGACTCAGGTCACCTCCTGAAGCCCTCGTTCAGGGT CGATATCCCCACATCAAGGACGGTGAGGATCTTAAAGACCACTCAACAGAAAGTAAAAAAATGGAAAATT GTCTAGGAGAATCCAGGCATGAAGTAGAAAAATCAGAAATCAGTGAAAACACAGATGCTTCGGGCAAAAT AGAGAAATATAATGTTCCGCTGAACAGGCTTAAGATGATGTTTGAGAAAGGTGAACCAACTCAAACTAAG ATTCTCCGGGCCCAAAGCCGAAGTGCAAGTGGAAGGAAGATCTCTGAAAACAGCTATTCTCTAGATGACC TGGAAATAGGCCCAGGTCAGTTGTCATCTTCTACATTTGACTCGGAGAAAAATGAGAGTAGACGAAATCT GGAACTTCCACGCCTCTCAGAAACCTCTATAAAGGATCGAATGGCCAAGTACCAGGCAGCTGTGTCCAAA CAAAGCAGCTCAACCAACTATACAAATGAGCTGAAAGCCAGTGGTGGCGAAATCAAAATTCATAAAATGG AGCAAAAGGAGAATGTGCCCCCAGGTCCTGAGGTCTGCATCACCCATCAGGAAGGGGAAAAGATTTCTGC AAATGAGAATAGCCTGGCAGTCCGTTCCACCCCTGCCGAAGATGACTCCCGTGACTCCCAGGTTAAGAGT GAGGTTCAACAGCCTGTCCATCCCAAGCCACTAAGTCCAGATTCCAGAGCCTCCAGTCTTTCTGAAAGTT CTCCTCCCAAAGCAATGAAGAAGTTTCAGGCACCTGCAAGAGAGACCTGCGTGGAATGTCAGAAGACAGT CTATCCAATGGAGCGTCTCTTGGCCAACCAGCAGGTGTTTCACATCAGCTGCTTCCGTTGCTCCTATTGC AACAACAAACTCAGTCTAGGAACATATGCATCTTTACATGGAAGAATCTATTGTAAGCCTCACTTCAATC AACTCTTTAAATCTAAGGGCAACTATGATGAAGGCTTTGGGCACAGACCACACAAGGATCTATGGGCAAG CAAAAATGAAAACGAAGAGATTTTGGAGAGACCAGCCCAGCTTGCAAATGCAAGGGAGACCCCTCACAGC CCAGGGGTAGAAGATGCCCCTATTGCTAAGGTGGGTGTCCTGGCTGCAAGTATGGAAGCCAAGGCCTCCT CTCAGCAGGAGAAGGAAGACAAGCCAGCTGAAACCAAGAAGCTGAGGATCGCCTGGCCACCCCCCACTGA ACTTGGAAGTTCAGGAAGTGCCTTGGAGGAAGGGATCAAAATGTCAAAGCCCAAATGGCCTCCTGAAGAC GAAATCAGCAAGCCCGAAGTTCCTGAGGATGTCGATCTAGATCTGAAGAAGCTAAGACGATCTTCTTCAC TGAAGGAAAGAAGCCGCCCATTCACTGTAGCAGCTTCATTTCAAAGCACCTCTGTCAAGAGCCCAAAAAC TGTGTCCCCACCTATCAGGAAAGGCTGGAGCATGTCAGAGCAGAGTGAAGAGTCTGTGGGTGGAAGAGTT GCAGAAAGGAAACAAGTGGAAAATGCCAAGGCTTCTAAGAAGAATGGGAATGTGGGAAAAACAACCTGGC AAAACAAAGAATCTAAAGGAGAGACAGGGAAGAGAAGTAAGGAAGGTCATAGTTTGGAGATGGAGAATGA GAATCTTGTAGAAAATGGTGCAGACTCCGATGAAGATGATAACAGCTTCCTCAAACAACAATCTCCACAA GAACCCAAGTCTCTGAATTGGTCGAGTTTTGTAGACAACACCTTTGCTGAAGAATTCACTACTCAGAATC AGAAATCCCAGGATGTGGAACTCTGGGAGGGAGAAGTGGTCAAAGAGCTCTCTGTGGAAGAACAGATAAA GAGAAATCGGTATTATGATGAGGATGAGGATGAAGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 4 EPLIN (LIMA1) (NM_016357) Human Tagged ORF Clone – RG211066

Protein Sequence: >RG211066 representing NM_016357 Red=Cloning site Green=Tags(s)

MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENLSQHFRKGTLT VLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQG RYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTK ILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSK QSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKS EVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYC NNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHS PGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPED EISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRV AERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQ EPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE

TRTRPLE - GFP Tag - V Restriction Sites: SgfI-MluI Cloning Scheme:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 4 EPLIN (LIMA1) (NM_016357) Human Tagged ORF Clone – RG211066

Plasmid Map:

ACCN: NM_016357 ORF Size: 2277 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_016357.5 RefSeq Size: 3664 bp RefSeq ORF: 2280 bp Locus ID: 51474 UniProt ID: Q9UHB6 Domains: LIM Gene Summary: This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]