CURRICULUM VITAE

Name: Myung Hee Park

Education:

1976 Ph.D. Brown University, Providence, RI: chemistry, 1972 B.S. Seoul National University, Seoul, Korea: chemistry,

Brief Chronology of Employment:

1998-present Chief, Molecular and Cellular Biochemistry Section, OPCB, NIDCR, NIH 2009-present Research Chemist, GS-15 Oral and Pharyngeal Cancer Branch, NIDCR, NIH 1996-2009 Research Chemist, GS-14 Oral and Pharyngeal Cancer Branch, NIDCR, NIH 1989-1996 Research Chemist, GS-13 Laboratory of Cellular Development and Oncology, NIDR, NIH 1984-1989 Senior Staff Fellow, Laboratory of Oral Biology and Physiology, NIDR, 1982-1984 Staff Fellow, Laboratory of Biochemistry, NIDR, NIH 1979-1982 Visiting Fellow, Laboratory of Biochemistry, NIDR, NIH 1976-1978 Research Associate, Department of Biology, Massachusetts Institute of Technology

Honors and Awards:

2005 Young Mae Award for Scientific Achievement 2000 CKD/ Society of Biomedical Research Award 1994 NIH Merit Award

Editorial Responsibilities:

2016-present Editorial Board member: Amino Acids 2010-present Editorial Board member: International Journal of Biochemistry and Molecular Biology 1999- 2008 Editorial Board Member: Archives of Pharmacal Research 1997-1998 Editorial Board Member: Journal of Biomedical Research

Professional Membership and Associations:

The American Society for Biochemistry and Molecular Biology Korean Scientists and Engineers Association Society for Biomedical Research

Extramural activities:

1985 Ad Hoc Review Member, Site Visit, University of Arizona 1987 Ad Hoc Review Member, Site Visit, Harvard Medical School 1994 Grant Review Board Member, Korean Science Foundation

1 1995-2000 Organizing Committee, Annual meeting of the Society of Biomedical Research 2001 Consultant, NIH CSR Study Section 2003 Grant Review Board Member, FY2003 USAMRMC/CDMRP Breast Cancer Research Program 2004 Chairperson, Organizing Committee, 14th Annual Meeting of the Society of Biomedical Research 2012 Scientific Advisory Board, International Congress on Polyamines: Biological and Clinical Perspectives. Istanbul, Turkey 2014 Scientific Advisory Board, Third International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives. Itamambuca Eco Resort. Ubatuba-SP, Brazil 2015 Organizing Committee, Inaugural Symposium on US NIH-KOREA Collaborative Biomedical Research 2016 Scientific Advisory Board, Fourth International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives, Tivoli Italy

Invited Lectures and Symposium Presentations:

2016 The fourth International Conference on Polyamines: Biochemical, physiological and clinical perspectives, Sept 4-9, 2016, Tivoli, Italy 2014 The Third International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives, Sept 2, 2014, Ubatuba, Brazil 2014 A keynote Speaker at the Annual Meeting of KWiSE (Korean Women in Science and Engineering) Eastern Chapter, Apr 19, 2014 2013 Medical University of South Carolina, Charleston, SC, Nov 12, 2013 2013 Gordon Research Conference on Polyamines, Waterville Valley, NH, June 17, 2013 2013 The Korean Society of Biochemistry and Molecular Biology Annual Meeting, May 16, 2013, Seoul, Korea 2013 The Catholic University of Korea, May 13, 2013, Seoul Korea 2012 International Congress on Polyamines, Istanbul, Turkey, Sept 4, 2012 2011 Annual Bioscience and Engineering Symposium, Vienna, VA, Nov 5, 2011 2011 College of Medicine, Catholic University of Korea, Seoul, Korea, Aug 11, 2011 2011 National Cancer Center, Goyang-Si, Gyeonggi-do, Korea, Aug 12, 2011 2010 International Conference on the Role of Polyamines and their Analogs in Cancer and Other Diseases, Tivoli, Italy, Dec 3, 2010 2010 the International Polyamine Conference: Progress in medicine and life sciences, June 16, 2010, at Gotemba Kogen, Japan 2010 Gyeongsang National University, School of Medicine, Jinju, Korea, June 21, 2010 2010 Gyeongsang National University, Environmental Biotechnology National Core Research Center, Jinju, Korea, June 21, 2010 2010 Dept of Life Sciences, Dongguk University, Seoul Korea, June 23, 2010 2009 International Congress on Amino Acids, Peptides and Proteins, Aug 2009, Vienna, Austria 2009 Gordon Research Conference on Polyamines, June 2009, Waterville Valley, NH 2008 Forty years of mammalian ornithine decarboxylase, June 2008, Kuopio, Finland 2008 US Korea Conference, Bioscience and Technology Symposium, Aug 2008, San Diego, CA

2 2007 International Congress on Biogenic Amines: Biological and Clinical Perspectives, Catania, Italy 2007 Bioscience and Technology symposium, US Korea Conference 2007, Reston, VA 2007 Sao Paulo State University, Araraquara, Brazil 2007 36th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq) 2007 University of Sao Paulo, Sao Paulo, Brazil 2006 Ewha Woman’s University, Seoul, Korea 2006 Seoul National University, Seoul Korea 2006 Korea Institute of Radiological and Medical Sciences 2006 International Conference on the Role of Polyamines and their Analogs in Cancer and Other Diseases, Tivoli (Rome), Italy 2006 US Korea Conference, Teaneck, NJ 2006 UMDNJ New Jersey Medical School 2005 NIH Korean Scientists Association, NIH 2005 Laboratory of Biochemistry and Genetics, NIDDK, NIH 2004 Gordon Research Conference on Polyamines, New London, Connecticut 2004 International Conference on Polyamines, Kazusa Arc, Japan 2003 The US Korea Conference, Pasadena, CA 2002 Annual Meeting of the Japanese Society of Biochemistry, Kyoto, Japan 2002 Korean National Institute of Health, Seoul, Korea Skin Research Institute, Pacific R & D Center, Yongin, Korea Seoul National University, School of Medicine, Seoul, Korea Catholic University of Korea, Cancer Research Center, Seoul, Korea Catholic University of Daegu, Daegu, Korea 1999 Gordon Research Conference on Polyamines, Oxford, United Kingdom 1998 Cancer Research Institute, Catholic University, Seoul, Korea Key Myung University Medical School, Taegu, Korea Kangnung National University College of Dentistry, Kangnung, Korea 1996 Workshop on eIF-5A Function, Genetic Therapy, Inc. Gaithersburg, MD Tokyo International Symposium on Polyamines, Tokyo, Japan Tokyo Medical and Dental University, Tokyo, Japan Seoul National University, Seoul, Korea Duk-Sung Women’s College, Seoul Korea LBG, NIDDK, NIH, Bethesda MD LMCB, NIDDK, NIH, Bethesda, MD 1995 Korean Green Cross Corporation, Yongin, Korea Korean National Institute of Health, Seoul, Korea Schering-Plough Institute, Kenilworth, NJ Sandoz Research Institute, Vienna, Austria International Congress on Amino Acids, Vienna, Austria Gordon Conference on Polyamines, Meriden NH 1994 Meeting of the Society of Biomedical Research Eisai Research Institute, Andover, MA 1993 Merck Sharp & Dohme Research Laboratory, WestPoint, PA University of Maryland, School of Pharmacy, Baltimore, MD

3 1990 Seoul National University, Seoul, Korea International Symposium on Polyamines in Molecular and Medical Biology, Kyoto, Japan 1989 Gordon Conference on Polyamines, Newport. RI Howard University, Washington, DC 1988 University of Rome, Rome, Italy 1988 International Symposium on Polyamines in Biochemical and Clinical Research, University of Naples, Naples, Italy 1986 Georgetown University, Medical School, Washington, DC 1984 FASEB Meeting, Mini-symposium on Polyamines, St. Louis, MO 1983 Pennsylvania State University, College of Medicine, Hershey, PA 1983 Gordon Conference on Polyamines, New London, NH

4 Intramural activities outside the NIDCR DIR:

2016 to Present President of KWiSE (Korean Women in Science and Engineering) NIH Chapter 2015 Organizing Committee of US NIH-Korea Collaborative Biomedical Research Symposium, Apr. 2015 2007 Review committee for the NIH National Graduate Student Research Festival 2006 Review committee for the NIH National Graduate Student Research Festival 2006 Grant Review Board Member (NIH, CSR), Molecular mechanism of Neurodegeneration 2005 Grant Review Board Member (NIH, CSR), Molecular mechanism of Neurodegeneration 2003 President, NIH Korean Scientists Association 2001 Search Committee, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute 2001 Consultant, NIH CSR Study Section 2001 President of NIH KSA (Korean Scientists Association)

Activities within the NIDCR DIR:

2012 NIDCR Summer Dental Student Award Committee 2011 NIDCR Award Committee 2004 Search Committee for a senior scientist, NIDCR, NIH 1997-1999 NIDCR Animal Care and Use Committee 1998 BSC Report Format Committee 1998 NIDR Equipment Committee 1997 NIDR DIR Seminar Committee 1997 NIDR Discretionary Funds Committee 1993-1994 NIDR Visiting Scholars Committee 1992-1996 NIDR Safety Committee

List of Invention Reports and Patents:

US Patent No 5,344,846 September 6, 1994 Jakus J, Park MH, Wolff EC. and Folk JE (1994) Compositions and methods for inhibiting deoxyhypusine synthase and the growth of cells. US Patent No. 7,141,589 November 28, 2006 Park MH, Clement PMJ, Hanauske-Abel HM, Wolff EC, Kleinman HK and Cracchiolo BM (2003) Method of inhibiting formation of vascular channels and methods of inhibiting proliferation. International Publication Number WO 03/018014; International Publication Date 06.03.2003 Priority Date August 23 2001; International Patent Application under the Patent Cooperation Treaty (PCT) International Application Number PCT/US02/26909 Filing Date August 23.2002

5 US Application No 10/527,453 US Provisional Patent Application No PCT/US03/28742, Sept 13, 2003; 601-1-135PCT WO 2004/024087 25 March 2004; Hanauske-Abel HM, Popowicz A, Wolff EC, Clement PMJ, Park MH, Cracchiolo BM. Methods of Diagnosing and Treating Hyperproliferative Disorders E308-2033/3-PCT-02 US Provisional Patent Application No PCT W02005/055931A2) December 2003 Hanauske- Abel HM, Palumbo P, Cracchiolo BM, Park MH, Wolff EC, Hanauske A-R and McLendon G. Method of preventing survival of retrovirally infected cells and of inhibiting formation of infectious retroviruses US Provisional Patent Application No PCT/US2005/035665 WO2006039607 April 13 2006 December 2004 Hanauske-Abel HM, Palumbo P, D’Alliessi DG, Mathews MB, Saxena D, Hoque M, Cracchiolo BM, Park MH, Hanauske A-R, Wolff EC, Pe’ery T. Method of improving drugs with anti-retroviral and anti-cancer activity. E-079-2007/0-US-01 US Provisional Patent Application No PCT 60/748,879 December 9 2006 December 9, 2005. Park, MH, Wolff, EC, Park, J-H, Hanauske-Abel HM. Deoxyhypusine hydroxylase E­ 051-2006/0-US-01

PUBLICATIONS

1. Lusk JE and Park MH. (1975) Phospholipase activity plays no role on the action of colicin K. Biochim. Biophys. Acta 394: 129-134. PMID 1095066 2. Park MH, Wong BB and Lusk JE. (1976) Mutants in three genes affecting transport of magnesium in Escherichia coli.: Genetics and physiology. J. Bacteriology 126: 1096­ 1103. PMID 780341 PMC 233130 3. Park MH, Berg WH and Buchanan JM (1979) The formation of plasminogen activator during viral transformation of chick embryo fibroblasts. Chem. Phys. of Human Plasma Proteins 315-328. 4. Folk JE, Park MH, Chung SI, Schrode J, Lester EP and Cooper HL (1980) Polyamines as physiological substrates for transglutaminases. J. Biol. Chem. 255: 3695- 3700. PMID 6102569 5. Park MH, Cooper HL and Folk JE (1981) Identification of hypusine, an unusual amino acid, in a protein from human lymphocytes and of spermidine as its biosynthetic precursor. Proc. Natl. Acad. Sci. USA 78:2869-2873. PMID 6789324 PMC 319460 6. Park MH, Cooper HL and Folk JE (1982) The biosynthesis of protein-bound hypusine [Nε-(4-amino-2-hydroxybutyl)lysine]: lysine as the amino acid precursor and the intermediate role of deoxyhypusine [Nε-(4-aminobutyl)lysine]. J. Biol. Chem. 257: 7217­ 7222. PMID 6806267 7. Cooper HL, Park MH and Folk JE (1982) Posttranslational formation of hypusine in a single major protein occurs generally in growing cells and is associated with activation of lymphocyte growth. Cell 29:791-797. PMID 6817926 8. Park MH, Cooper HL and Folk JE (1983) Chromatographic identification of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] and deoxyhypusine [Nε-(4- aminobutyl)lysine]. Methods in Enzymology 94: 458-462.

6 9. Cooper HL, Park MH, Folk JE, Safer B and Braverman R (1983) Identification of the hypusine-containing protein Hy+ as translation initiation factor eIF-4D. Proc. Natl. Acad. Sci. USA 80: 1854-1857. PMID 6403941 PMC 393708 10. Cooper HL, Park MH and Folk JE (1984) Hypusine formation: A unique posttranslational modification of translation initiation factor eIF-4D. Methods in Enzymology 106: 344-351. PMID: 6436642 11. Park MH, Chung SI, Cooper HL and Folk JE (1984) The mammalian hypusine­ containing protein, eIF-4D. structural homology of this protein from several species. J. Biol. Chem. 259: 4563-4565. PMID: 6423643 12. Park MH, Liberato DJ, Yergey AL and Folk JE (1984) The biosynthesis of hypusine [Nε­ (4-amino-2-hydroxybutyl)lysine]: alignment of the butylamine segment and source of the secondary amino nitrogen. J. Biol. Chem. 259: 12123-12127. PMID 6434537 13. Abbruzzese A, Park MH and Folk JE (1986) Deoxyhypusine hydroxylase from rat testis: partial purification and characterization. J. Biol. Chem. 261: 3085-3089. PMID 3949761 14. Abbruzzese A, Park MH and Folk JE (1986) Indirect assays for deoxyhypusine hydroxylase using dual-label ratio changes and oxidative release of radioactivity. Anal. Biochem. 154: 664-670. PMID 3089062 15. Park MH and Folk JE (1986) Biosynthetic labeling of hypusine in mammalian cells: carbon-hydrogen bond fissions revealed by dual-labeling. J. Biol. Chem. 261: 14108­ 14111. PMID 3095314 16. Park MH, Liu TY, Neece SH and Swiggard WJ (1986) Eukaryotic initiation factor 4D: purification from human red blood cells and the sequence of amino acids around its single hypusine residue. J. Biol. Chem. 261: 14515-14519. PMID 3095320 17. Abbruzzese A, Park MH and Folk JE (1987) Hypusine biosynthesis: studies on deoxyhypusine hydroxylase. Italian J. Biochem. 36: 45A-48A. 18. Park MH (1987) Regulation of biosynthesis of hypusine in Chinese hamster ovary cells: evidence for eIF-4D precursor polypeptides. J. Biol. Chem. 262: 12730-12734. PMID 3114263 19. Park MH, Abbruzzese A and Folk JE (1988) Post-translational formation of hypusine: biogenesis of translation initiation factor eIF-4D. In: Zappia, V., Galletti, P., Porta, R. and Wold, F. eds: Advances in Post-translational Modifications of Protein and Aging. Plenum Press, pp 633-640. PMID 3137791 20. Abbruzzese A, Liguori V, Isernia T and Park MH (1988) Inhibition of deoxyhypusine hydroxylase by polyamines. Italian J. Biochem. 37: 187A-189A. 21. Park MH (1988) Identification of an eukaryotic initiation factor 4D precursor in spermidine-depleted Chinese hamster ovary cells. J. Biol. Chem. 263: 7447-7449. PMID 3131321 22. Park MH and Wolff EC (1988) Cell-free synthesis of deoxyhypusine: separation of protein substrate and enzyme, and identification of 1,3-diaminopropane as a product of spermidine cleavage. J. Biol. Chem. 263: 15264-15269. PMID 3139668 23. Park MH, Wolff EC, Abbruzzese A and Folk JE (1988) Biosynthesis of hypusine in eIF­ 4D precursors. In: Zappia V and Pegg AE eds. Progress in Polyamine Research. New York, NY: Plenum Press, pp. 435-447. PMID 3151229 24. Abbruzzese A, Liguori V and Park MH (1988) Deoxyhypusine hydroxylase. In: Zappia, V and Pegg AE, eds. Progress in Polyamine Research. New York, NY: Plenum Press, Adv. in Exper. Med. and Biol. V250 pp. 459-466. PMID 3076334

7 25. Abbruzzese A, Park MH, Beninati S and Folk JE (1989) Inhibition of deoxyhypusine hydroxylase by polyamines and by a deoxyhypusine peptide. Biochim. Biophys. Acta 997: 248-255. PMID 2765563 26. Park MH (1989) The essential role of hypusine in eukaryotic translation initiation factor 4D (eIF-4D): purification of eIF-4D and its precursors and comparison of their activities. J. Biol. Chem. 264: 18531-18535. PMID 2509461 27. Wolff EC, Park MH and Folk JE (1990) Cleavage of spermidine as the first step in deoxyhypusine synthesis. J. Biol. Chem. 265: 4793-3799. PMID 2108161 28. Abbruzzese A. Hanauske-Abel HM, Park MH, Henke S and Folk JE (1991) The active site of deoxyhypusyl hydroxylase: use of catecholpeptides and their component chelator and peptide moieties as molecular probes. Biochim. Biophys. Acta 1077:159-166. PMID 2015290 29. Chung SI, Park MH, Folk JE and Lewis MS (1991) Eukaryotic initiation factor 5A: the molecular form of the hypusine-containing protein from human erythrocytes. Biochim. Biophys. Acta 1076: 448-451. PMID1900436 30. Park MH, Wolff EC, Smit-McBride Z, Hershey JWB and Folk JE (1991) Comparison of the activities of variant forms of eIF-4D: the requirement for hypusine or deoxyhypusine. J. Biol. Chem. 266: 7988-7994. PMID 1850732 31. Wolff EC, Kinzy TG, Merrick WC and Park MH (1992) Two isoforms of eIF-5A in chick embryo: isolation, activity and comparison of sequences of the hypusine-containing proteins. J. Biol. Chem. 267:6107-6113. PMID 1556119 32. Park MH, Wolff EC, and Folk JE (1993) Review. Hypusine: its post-translational formation in eukaryotic initiation factor 5A and its potential role in cellular regulation. BioFactors 4: 95-104. PMID: 8347280 33. Jakus J, Wolff EC, Park MH and Folk JE (1993) Features of the spermidine-binding site of deoxyhypusine synthase as derived from inhibition studies: effective inhibition by bis- and mono-guanylated diamines and polyamines. J. Biol. Chem. 268: 13151-13159. PMID: 8514754 34. Rinaudo MS, Joe YA and Park MH (1993) Cloning and sequencing of a chick embryo cDNA encoding the 20-kDa hypusine-containing protein, eIF-5A. Gene 137: 303-307. PMID: 7916728 35. Park MH, Wolff EC and Folk JE (1993) Review. Is hypusine essential for eukaryotic cell proliferation? Trends in Biochemical Sciences 18: 475-479. PMID: 8108861 36. Hanauske-Abel, HM, Park MH, Hanauske A-R, Popowicz AM, Lalande M and Folk JE (1994) Inhibition of G1-S transition by inhibitors of deoxyhypusine hydroxylation. Biochem. Biophys. Acta.1221: 115-124. PMID 8148388 37. Joe YA and Park MH (1994) Structural features of the eIF-5A precursor required for post-translational synthesis of deoxyhypusine. J. Biol. Chem. 269: 25916-25921. PMID 7929297 38. Park MH, Wolff EC, Lee YB and Folk JE (1994) Antiproliferative effects of inhibitors of deoxyhypusine synthase: inhibition of growth of Chinese hamster ovary cells by guanyl diamines. J. Biol. Chem. 269: 27827-27832. PMID 7961711

8 39. McCaffrey TA, Pomerantz KB, Sanborn TA, Spokojny AM. Du B, Park, MH, Folk JE, Lamberg A, Kivirikko KI, Falcone DJ, Mehta SB and Hanauske-Abel HM (1995) Specific inhibition of eIF-5A and collagen hydroxylation by a single agent: antiproliferative and fibrosuppressive effects on smooth muscle cells from human coronary arteries. J. Clin. Invest. 95: 446-455. PMID: 7860726 40. Wolff EC, Lee YB, Chung SI, Folk JE and Park MH (1995) Deoxyhypusine synthase from rat testis: purification and characterization. J. Biol Chem. 270: 8660-8666. PMID: 7721768 41. Lee YB, Wolff EC, Park MH and Folk JE (1995) Diamine and triamine analogs and derivatives as inhibitors of deoxyhypusine synthase: synthesis and biological activity. J. Med. Chem. 38: 3053-3061. PMID: 7636868 42. Kang KR, Wolff EC, Park MH, Folk JE. and Chung SI (1995) Identification of YHR068w in Saccharomyces cerevisiae as a gene for deoxyhypusine synthase: expression and characterization of the enzyme. J. Biol. Chem. 270: 18408-18412. PMID: 7629166 43. Joe YA, Wolff EC and Park MH (1995) Cloning and expression of human deoxyhypusine synthase cDNA: structure-function studies with the recombinant enzyme and mutant proteins. J. Biol. Chem. 270: 22386-22393. PMID: 7673224 44. Lee YB, Joe YA and Park MH (1995) Inhibitors of hypusine biosynthesis: potential anticancer agents. J. Biomed. Res. 5: 46-52 45. Park MH, Joe YA, Kang KR, Lee YB and Wolff EC (1996) The polyamine-derived amino acid hypusine: its posttranslational formation in eIF-5A and its role in cell proliferation. Amino Acids 10: 109-121. PMID 24178473 doi: 10.1007/BF00806584. 46. Lee YB and Park MH (1996) Inhibitors of deoxyhypusine synthase: structural features and biological study. Proc. 7th KSEA Northeast Regional Conf. (1996): pp 95-99 47. Wolff EC, Folk JE and Park MH (1997) Enzyme-substrate intermediate formation at lysine-329 of human deoxyhypusine synthase. J. Biol. Chem. 272: 15865-15871 PMID 9188485 48. Park MH, Lee YB and Joe YA (1997) Hypusine is essential for eukaryotic cell proliferation. Biol. Signals 6: 115-123 PMID: 9285094 49. Joe YA, Wolff EC, Lee YB and Park MH (1997) Enzyme-substrate intermediate at a specific lysine residue is required for deoxyhypusine synthesis: the role of Lys329 in human deoxyhypusine synthase J. Biol. Chem. 272: 32679-32685 PMID 9405486 50. Park MH, Joe YA and Kang KR (1998) Deoxyhypusine synthase activity is essential for cell viability in the yeast Saccharomyces cerevisiae J. Biol. Chem. 273: 1677-1683 PMID 9430712 51. Liao D-I, Wolff EC, Park MH and Davies DR (1998) Crystal Structure of the NAD complex of human deoxyhypusine synthase: an enzyme with a ball and chain mechanism for blocking the active site. Structure 6: 23-32 PMID9493264 52. Wolff EC and Park MH (1999) Identification of Lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation. Yeast 15: 43-50 PMID10028184 53. Lee YB, Joe YA, Wolff EC, Dimitriadis EK and Park MH (1999): Complex formation between human deoxyhypusine synthase and its protein substrate, the eIF5A precursor Biochem. J. 340: 273-281 PMID 10229683 PMC 1220246 54. Wolff EC, Wolff J and Park MH (2000): Deoxyhypusine synthase generates and uses bound NADH in a transient hydride transfer mechanism. J. Biol. Chem. 275, 9170-9177 PMID: 10734052

9 55. Lee CH, Marekov LN, Kim SY, Brahim JS, Park MH and Steinert PM (2000) Small proline-rich protein 1 is a major component of cornified cell envelope of normal human oral keratinocytes FEBS Lett. 477, 268-272 PMID10908733 56. Lee CH and Park MH (2000) Human deoxyhypusine synthase: Interrelationship between the binding of NAD and substrates Biochem. J. 352, 851-857 PMID 11104695 57. Lee CH, Um P and Park MH (2001) Structure/function studies of human deoxyhypusine synthase: Identification of amino acids critical for the binding of spermidine and NAD. Biochem. J. 355, 841-849 PMID 11311149 58. Lee Y, Kim HK, Kim YY, Park MH and Joe YA (2002) Effects of N1-guanyl-1,7­ diaminoheptane, an inhibitor of deoxyhypusine synthase, on endothelial cell growth, differentiation and apoptosis. Mol. Cell. Biochem. 237 (1-2), 69-76 PMID 12236588 59. Clement PMJ, Hanauske-Abel HM, Wolff EC, Kleinman HK and Park MH (2002) The antifungal drug ciclopirox inhibits deoxyhypusine and proline hydroxylation, endothelial cell growth and angiogenesis in vitro. Int. J. Cancer 100, 491-498 PMID: 12115536 60. Kang KR, Kim J.S, Chung SI, Park MH, Kim Y W, Lim D and Lee S.Y (2002) Deoxyhypusine synthase is phosphorylated by protein kinase C in vivo as well as in vitro. Exp Mol Med 34, 489-495 PMID 12526092 61. Leethanakul C, Knezevic V, Patel V, Amornphimoltham P, Gillespie J, Shillitoe EJ, Emko P, Park MH, Emmert-Buck MR, Strausberg RL, Krizman DB and Gutkind JS (2003) Gene discovery in oral squamous cell carcinoma through the Head and Neck Cancer Genome Anatomy Project: confirmation by microarray analysis. Oral Oncology 39, 248-258 PMID 12618197 62. Park J-H, Wolff EC, Folk JE and Park MH (2003) Reversal of the deoxyhypusine synthesis reaction: Generation of spermidine or homospermidine from deoxyhypusine by deoxyhypusine synthase. J. Biol. Chem. 278, 32683-32691 PMID: 12788913 63. Clement PMJ, Henderson A, Jenkins ZA, Smit-McBride Z, Wolff EC, Hershey JWB., Park MH and Johansson HE (2003) Identification and characterization of eukaryotic initiation factor 5A-2. Eur. J. Biochem 270, 4254-4263 64. Cracchiolo BM, Heller DS, Clement PMJ, Wolff EC, Park MH and Hanauske-Abel HM (2004) Aberrant expression of the hypusine-containing eukaryotic initiation factor 5A (eIF5A) in intraepithelial neoplasia of the vulva. Gynecologic Oncology 94, 217-222 65. Jeon GA, Lee J-S, Patel V, Gutkind JS, Thorgeirsson S, Kim EC, Chu I-S and Park MH (2004) Global gene expression profile of human head and neck squamous carcinoma cell lines. Int. J. Cancer 112, 249-258 66. Umland TC, Wolff EC, Park MH and Davies DR (2004) A new crystal structure of deoxyhypusine synthase reveals the configuration of the active site enzyme and of an enzyme:NAD:inhibitor ternary complex. J. Biol. Chem. 279, 28697-28705 67. Nishimura K, Murozumi K, Shirahata A, Park MH, Kashiwagi K and Igarashi K (2005) Independent roles of eIF5A and polyamines in cell proliferation. Biochem. J. 385, 779­ 785 PMC1134754 68. Kim, E-C, Hwang Y-S, Lee, Lee, H-J, Lee, S-K, Jeon, C-D, Lee, S-K, Park, MH, Yu H­ H. and You, Y-O (2005) Caesalpinia sappan induces cell death by increasing the expression of p53 and p21AF1/CIP1 in head and neck cancer cells. Amer. J. Chinese Med. 33, 405-414

10 69. Dong Z, Arnold RJ, Mechref YS, Novotny MV, Park MH and Zhang J-T (2005) Modulation of DRG-1 expression at both transcriptional and post-transcriptional levels, revealed by cell cycle blocker mimosine and proteomic analysis Mol. Cell. Proteomics 4.7, 993-1001 PMID 15855174 70. Lee HJ, Guo HY, Lee SK, Jeon BH, Jun CD, Lee SK, Park MH and Kim EC (2005) Effects of nicotine on proliferation, cell cycle and differentiation in immortalized and malignant oral keratinocytes. J. Oral. Pathol. Med. 34, 436-443 PMID 16011614 71. Park, J-H, Aravind, L, Wolff, EC, Kaevel, J, Kim, YS and Park MH (2006) Molecular cloning, expression and structural prediction of deoxyhypusine hydroxylase: a novel HEAT-repeat-containing metalloenzyme, Proc. Natl. Acad. Sci. USA 103, 51-56. PMID: 16371467 PMC1324997 72. Clement, PMJ, Johansson, HE, Wolff, EC and Park MH (2006) Differential expression of eIF5A-1 and eIF5A-2 in human cancer cells. FEBS J. 273, 1102-1114 PMID: 16519677 73. Kim YS, Kang KR, Wolff EC, Bell JK, McPhie P and Park MH (2006) Deoxyhypusine hydroxylase is a Fe(II)-dependent HEAT-repeat enzyme: Identification of amino acid residues critical for Fe(II) binding and catalysis. J. Biol. Chem. 281, 13217-13225 PMID 16533814 PMC1868894 74. Park MH (2006) The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A). J. Biochem. 139, 161-169 PMC2494880 75. Kang KR, Kim YS, Wolff EC and Park MH (2007) Specificity of the deoxyhypusine hydroxylase-eIF5A interactions: Identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A. J. Biol. Chem. 282, 8300-8308 PMID: 17213197 PMC1852541 76. Wolff EC, Kang KR, Kim YS and Park MH (2007) Posttranslational synthesis of hypusine: evolutionary progression and specificity of the hypusine modification. Amino Acids. 33, 341-350 PMID: 17476569 PMC2572820 77. Huang Y, Higginson DS, Hester L, Park MH and Snyder SH. (2007) Neuronal growth and survival mediated by eIF5A, a polyamine-modified translation initiation factor. Proc. Natl. Acad. Sci. USA 104, 4194-4199 PMC1820731 78. Cano VSP, Jeon GA, Johansson HE, Henderson JA, Park JH, Valentini SR, Hershey JWB. and Park MH (2008) Mutational analyses of human eIF5A-1: Identification of amino acid residues critical for hypusine modification and eIF5A activity. FEBS J. 275, 44-58 PMID: 18067580 PMC2536608 79. Dias CAO, Cano VSP, Rangel SM, Apponi LH, Frigieri MC, Muniz RCC, Garcia W, Park MH, Garratt RC, Zanelli CF and Valentini SR (2008) Structural modeling and mutational analysis of yeast eIF5A reveal new residues and reinforce its involvement in protein synthesis. FEBS J. 275, 1874-1888 PMID: 18341589 80. Chattopadhyay MK, Park MH and Tabor H (2008) Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine. Proc. Natl. Acad. Sci. USA 105, 6554-6559 PMID: 18451031 PMC2373341 81. Lee SB, Park JH, Kaevel JK, Sramkova M, Weigert R and Park MH (2009) The effect of hypusine modification on the intracellular localization of eIF5A, Biochem. Biophys. Res. Commun. 383, 497-502 PMID 19379712 PMC2737708

11 82. Vu VV, Emerson JP, Martinho M, Kim YS, Munck E, Park MH and Que L (2009) Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O2 with a nonheme diiron center. Proc. Natl. Acad. Sci. USA, 106, 14814­ 14819 PMID 19706422 PMC2736468 83. Hoque M, Hanauske-Abel HM, Palumbo P, Saxena D, Gandolfi DD, Park MH, Pe’ery T and Mathews MB (2009) Inhibition of HIV-1 gene expression by ciclopirox and deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A. Retrovirology Oct 13;6:90 PMID 19825182 PMC2770518 84. Chawla B, Jhingran A, Singh S, Tyagi N, Park MH, Srinicasan N, Roberts SC and Madhubala R (2010) Identification and characterization of a novel deoxyhypusine synthase in Leishmania donovani. J. Biol. Chem. 285: 453-463 PMID 19880510 PMC2804193 85. Landau GB, Bercovich Z, Park MH and Kahana C (2010) The role of polyamines in supporting growth of mammalian cells is mediated through their requirement for translation initiation and elongation. J. Biol. Chem. 285: 12474-12481 PMID 20181941 PMC2857121 86. Lee SB, Park JH, Woster PM, Casero RA and Park MH (2010) Suppression of exogenous gene expression by spermidine/spermine-N1-acetyltransferase (SSAT1) cotransfection. J. Biol. Chem. 285, 15548-15556 PMID 20212040 PMC2865325 87. Cano VSP, Medrano FJ, Park MH and Valentini SR (2010) Evidence for conformational changes of the yeast deoxyhypusine hydroxylase Lia1 upon iron displacement from its active site. Amino Acids 38, 479-490 PMID 19956996 PMC2901987 88. Park MH, Nishimura K, Zanelli CF and Valentini SR (2010) Functional significance of eIF5A and its hypusine modification in eukaryotes. Amino Acids 38, 491-500 PMID 19997760 PMC2829442 89. Wolff EC, Lee SB and Park MH (2011) Assay of deoxyhypusine synthase activity. Methods in Molecular Biology 720, 195-205 PMID 21318875 PMC3178833 90. Park JH, Wolff EC and Park MH (2011) Assay of deoxyhypusine hydroxylase activity. Methods in Molecular Biology 720, 207-216 PMID 21318876 PMC3178843 91. Lee SB, Park JH, Folk JE, Deck JA, Pegg AE, Sokabe M, Fraser CS and Park MH (2011) Inactivation of eukaryotic initiation factor 5A (eIF5A) by specific acetylation of its hypusine residue by spermidine/spermine acetyltransferase 1 (SSAT1). Biochem. J. 433, 205-213 PMID 20101322 PMC3003598 92. Park JH, Dias CAO, Lee SB, Valentini SR, Sokabe M, Fraser CS and Park MH (2011) Production of active recombinant eIF5A: reconstitution in E. coli of eukaryotic hypusine modification of eIF5A by its coexpression with modifying enzymes. Protein Eng. Des. Sel. 24, 301-309 PMID 21131325 PMC3038461 93. Yum S, Choi J, Hong S, Park MH, Lee J, Ha N-C and Jung Y (2011) Hyperoxia attenuates the inhibitory effect of nitric oxide donors on HIF prolyl-4- hydroxylase-2: Implication on discriminative action of nitric oxide on HIF prolyl-4-hydroxylase-2 and collagen prolyl-4- hydroxylase. Biochemical Pharmacology 82, 485-490 PMID 21723853 PMC3994879 94. Cerrada-Gimenez M, Weisell J, Hyvönen MT, Park MH, Alhonen L, Vepsäläinen J and Keinänen TA (2011) Complex N-acetylation of triethylenetetramine. Drug Metabolism and Disposition 39, 2242-2249 PMID: 21878558 PMC3226374

12 95. Park J-H, Johansson HE, Aoki H, Huang BX, Kim H-Y, Ganoza MC, Park MH (2012) Post-translational modification by β-lysylation is required for the activity of E. coli Elongation Factor P (EF-P). J. Biol. Chem. 287, 2579-2590 PMID: 22128152 PMC3268417 96. Hyvonen MT, Keinanen TA, Khomutov M, Simonian A, Vepsalainen J, Park JH, Khomutov AR, Alhonen L and Park MH (2012) Effects of novel C-methylated spermidine analogs on cell growth via hypusination of eukaryotic translation initiation factor 5A. Amino Acids 42, 685-695 PMID: 21861168 PMC3223563 97. Dias CAO, Gregio APB, Rossi D, Galvao FC, Watanabe TF, Park MH, Valentini SR and Zanelli CF (2012) eIF5A interacts functionally with eEF2. Amino Acids 42, 697-702, PMID: 21822730 PMC32445752 98. Nishimura K, Lee SB, Park JH and Park MH (2012) Essential role of eIF5A-1 and deoxyhypusine synthase in mouse embryonic development. Amino Acids 42, 703-710 PMID: 21850436 PMC3220921 99. Chawla B, Kumar RR, Tyagi N, Subramanian G, Srinivasan N, Park MH and Madhubala, RA (2012) Unique modification of the eukaryotic initiation factor 5A shows the presence of the complete hypusine pathway in Leishmania donovani. PLoS One. 7(3), e33138 PMID: 22438895 PMC3306375 100. Beninati S, Park MH, Wolff E, Fesus L, Abbruzzese A, Chung SI, Carmassi F, Cocuzze E, Trawick ML and Piacentini M (2013) In memoriam: John E Folk (1925-2010) Amino Acids 44, 11-18 PMID: 22847781 DOI10.1007/s00726-012-1367-y 101. Caraglia M, Park MH, Wolff EC, Marra M and Abbruzzese A (2013) eIF5A isoforms and cancer: two brothers for two functions? Amino Acids 44, 103-109 PMID 22139412 PMC3536922 102. Mandal S, Mandal A, Johansson HE, Orjalo AV and Park MH (2013) Depletion of cellular polyamines, spermidine and spermine, causes a total arrest in translation and growth in mammalian cells. Proc. Natl. Acad. Sci. USA 110, 2169-2174 PMID 23345430 PMC 3568356 103. Park MH and Igarashi K (2013) Polyamines and their metabolites as diagnostic markers of human diseases. Biomolecules & Therapeutics 21, 1-9 PMID 24009852 PMC 3762300 104. Hanauske-Abel HM, Saxena D, Palumbo PE, Hanauske A-R, Luchessi AD, Cambiaghi TD, Hoque M, Spino M, Gandolfi DD, Heller DS, Singh S, Park MH, Cracchiolo BM, Tricta F, Connelly J, Popowicz AM, Cone RA, Holland B, Pe’ery T and Mathews MB (2013) Drug-induced reactivation of apoptosis abrogates HIV-1 infection. PLoS ONE 8(9): e74414. doi:10.1371/journal.pone.0074414 PMC 3781084 105. Frey AG, Nandal A, Park JH, Smith PM, Yabe T, Ryu M-S, Ghosh MC, Lee J, Roualt TA, Park MH and Philpott CC (2014) Iron chaperones PCBP1 and PCBP2 mediate the metallation of the dinuclear iron enzyme deoxyhypusine hydroxylase Proc. Natl. Acad. Sci. USA 111, 8032-8036 PMID 24843120 PMC 4050543 106. Mittal M, Morada M, Tripathi P, Gowri VS, Mandal S, Quirch A, Park MH, Yarlett N and Madhubala R. (2014) Cryptosporidium parvum has an active hypusine biosynthesis pathway. Molecular and Biochemical Parasitology 195:14-22 doi: 10.1016/j.molbiopara.2014.05.005 PMID 24893338 PMC4176827 107. Park MH, Mandal S, Mandal A and Wolff EC. (2014) eIF5A and Cancer. In: Parsyan, A, ed. Translation and Cancer: Applications in Medicine, Springer. pp223-232. ISBN: 978­ 94-017-9077-2 (Print) 978-94-017-9078-9 (Online)

13 108. Mandal A, Mandal S and Park MH (2014) Genome-wide analyses and functional classification of proline repeat-rich proteins: potential role of eIF5A in eukaryotic evolution PLoS ONE 9(11): e111800 doi 10.1371/journal.pone.0111800 PMC 4218817 109. Wolff EC and Park MH. (2015) Role of the polyamine spermidine as a precursor for hypusine modification in eIF5A. In: Kusano, T and Suzuki, H, eds Polyamines: A universal molecular nexus for growth, survival and specialized metabolism, Springer Japan pp 121-129 ISBN 978-4-431-55211-6 110. Mandal S, Mandal A and Park MH (2015) Depletion of the polyamines spermidine and spermine by overexpression of spermidine/spermine N1-acetyltransferase 1 (SAT1) leads to mitochondria-mediated apoptosis in mammalian cells Biochem. J. 468(3): 435-447 doi: 10.1042/BJ20150168 PMID 25849284 111. Mandal A., Mandal S., Park M.H. (2016) Global quantitative proteomic analyses reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells. Scientific Reports 6:25795 | DOI: 10.1038/srep25795 online 5/16/16 112. Saxena D., Spino M., Tricta F., Connelly J., Cracchiolo B.M., Hanauske A.-R., Gandolfi D.D., Mathews M.B., Karn J., Holland B., Park M.H., Pe’ery T., Palumbo P.E. and Hanauske-Abel H.M. (2016) Drug-Based Lead Discovery: The Novel Ablative Antiretroviral Profile Of Deferiprone in HIV-1-Infected Cells and in HIV-Infected Treatment-Naive Subjects of A Double-Blind, Placebo-Controlled, Randomized Exploratory Trial. PLOS One DOI:10.1371/journal.pone.0154842 May 18, 2016 113. Xiao S., Chen Y., Buehler E., Mandal S., Mandal A., Betenbaugh M., Park M.H., Martin S. and Shiloach J. (2016) Genome-scale RNA interference screen identifies antizyme 1 (OAZ1) as a target for improvement of recombinant protein production in mammalian cells. Biotechnol Bioeng. 2016 May 23. doi: 10.1002/bit.26017. 114. Jasniewski A.J., Engstrom L.M., Vu V.V., Park M.H. and Que L.Jr. (2016) X-ray absorption spectroscopic characterization of the diferric-peroxo intermediate of human deoxyhypusine hydroxylase in the presence of its substrate eIF5A. J. Biol. Inorg. Chem. 2016 Jul 5, doi: 10.1007/s00775-016-1373-8

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