ISSN: 2 941 91 Indian Journal of Biology5 Volume 5 Number 1, January June 2018 Original Article DOI: http://d .doi.org/10.21088/ijb.2 94.1 91.5118.8
aematological Analysis o Leschenault s Lea oad Gecko, emidact l s esc ena ltii Dumeril and Bibron
Sarbes ar Nayak , Pra ulla Kumar Mohanty
Abstract
aematology is useful in understanding the physiological features of lizards. The study was held to investigate the haemo profile of leschenault s leaf toad gecko in Odisha. The haematological parameters like aemoglobin Concentration B , Packed Cell Volume PCV , Total Erythrocyte Count TEC , Mean Corpuscular Volume MCV , Mean Corpuscular aemoglobin MC , Mean Corpuscular aemoglobin Concentration MC C , Total Leucocyte Count TLC , Total Platelet Count TPC and Differential Leucocyte Count DLC were calculated using standard procedures. Statistical analysis like correlation and ttest were done. The study revealed that the TEC and percentage of lymphocytes show significant difference p 0.05 between both se es of lizard. The mean values of all other parameters are also showing difference between male and female of Hemidactylus leschenaultii Dumeril and Bibron 18 . The correlation coefficient varies in male and female with respect to the parameters analysed. Some parameters were positively correlated with each other and others were found to be negatively correlated. The data obtained could be a useful indicator for monitoring and managing the general health status of the species. Key ords aematological Parameters Hemidactylus Leschenaultii Correlation Significant Difference.
Introduction Author s A iliation Lecturer in Zoology OESII , Department of Zoology, Vikram Deb Autonomous College, Jeypore, Koraput, Odisha 4001, India. Professor, Postgraduate Department of Zoology, Utkal University, Vani The significant of haematological study for Vihar, Bhubaneswar, Odisha 51004, India. evaluating, analysing, e amining and controlling the health status of animals is a prelude blueprint. The Re rint s Re uest Sarbes ar Nayak, Lecturer in Zoology haematologic techni ue for assessment of body OESII , Department of Zoology, Vikram Deb Autonomous College, Jeypore, Koraput, Odisha 4001, India. physiology has a great role being uncomplicated, Email: nayaksarbeswar89 gmail.com, serene, minimal invasive, productive first hand prafulla.mohanty gmail.com techni ue and economically sustainable. Received on 29.01.2018, Acce ted on 09.02.2018 Leschenault s leaf toad gecko is belonging to Class– Reptilia, Order– S uamata and Family cell haemoglobin concentration were studied in some Gekkonidae, found in warm climatic conditions reptiles 10,11,12 . aematology in reptiles highly throughout the world 1 . aemocytological dependent on age as well as se and it varies parameters are useful and widely used tools that throughout life 1 ,14 . The blood cells were identified assist in the diagnosis and monitoring of animal and different parameters were taken 1520 . health 2 . The combinations of different haematological parameters detect the physiological The data about haematology of reptiles is still conditions and clinical evaluation in reptiles ,4 . challenging in comparison to other nonmammalian The different e ternal and internal factors also affect vertebrates. The literature available on haematology the haemoprofile of nonmammalian vertebrates 5, . of this lizard specifically from the studied aematolgy of some saurians were studied ,8,9 . geographical region of Odisha is very less. The The haemoglobin concentration, haematocrit value, comparative scarcity of informations regarding the mean cell volume, mean cell haemoglobin and mean haematology of geckos provides a less evidences about
Indian Journal of Biology / Volume 5 Number 1 / January June 2018 Red Flower Publication Pvt.Ltd 54 Sarbeswar Nayak & Prafulla Kumar Mohanty / aematological Analysis of Leschenault s Leaf Toad Gecko, Hemidactylus Leschenaultii Dumeril and Bibron 18 family Gekkonidae. The purpose of this study was to and stained with Giemsa as protocol cited by determine the reference intervals of haematology and Lillie 2 . morphometry of blood cells of normal and apparently Haematological Analysis healthy lizards. The findings will serve as base line data for future health assessment of lizards as well The blood parameters were studied using the as for conservation and protection. procedure 24,25,2 ,2 . The concentration of haemoglobin was estimated as o yhaemoglobin by Sahalis haemometer and e pressed in g , Packed Materials and Methods cell volume PCV was determined by microhaematocrit method with a spun of microhaematocrit tube at 2500 rpm for 15 minutes. The uantification of RBCs and Animals WBCs was performed by manual methods using Fifteen lizards of each se were collected from the haemocytometer, with ayem s diluting fluid for coastal area of Rajnagar block of Kendrapara 54 225, RBCs and Turk s diluting fluid for WBCs. Erythrocyte Odisha, located in 20 20 N to 20 N latitude and indices like MCV, MC and MC C were calculated 8 14 E to 8 01 E longitude. They were caught at day using standard formulae 28 . The percentage of time from the crevices of trees and sometimes at wall of different leucocytes as well as total platelet count boundaries and homes adjacent to the fields. Lizards determined 29, 0 . were clinically healthy and in good condition transferred to animal house. The investigation of haematological profiles on Hemidactylus leschenaultii Dumeril and Statistical Analysis Bibron 18 was carried out from 2014 to 201 . The data presented as Mean SE Standard Error for both se es and MS office E cel 200 was used for statistical analysis. The correlation analysis between Blood Collection the parameters and the significant difference P 0.05 The venipuncture site was prepared aseptically was taken using Student s ttest assuming e ual prior to blood collection. Blood was collected from variances with the help of Paleontological Statistics the ventral tail vein of lizards by inserting an insulin PAST version 2.1 Natural istory Museum, syringe BD Ultra – Fine TM Needle 12. mm 0G at University of Oslo . an angle of 45 0 between the scales on ventral midline 21,22 . Once blood appeared in the needle hub, held steady and a gentle negative pressure was Results applied to the syringe. The blood was kept in an EDTA vial and then transported in icebo to laboratory. The aematological parameters were analysed in case lizards were released to their natural habitat after of adult lizard Table1 . Assuming a confidence level collection of blood. Whole blood smear were obtained of 95 , significant differences were found in two by push slide techni ue, air dried, fi ed with methanol
able aematological parameters of H. Leschenaultii Dumeril and Bibron 18
Sl. Parameters nit Male emale P No. Range Mean S M Range Mean S M value
1 aemoglobin g 4.89 – 10. 5 .49 0.41 4.5 – 9. 4 .5 0. 1. 5 2 PCV 11. – 0. 21.25 1.4 11.89 – 0.89 20.12 1. 9 0.54 TEC 10 mm 0.8 – 2. 4 1.4 0.14 0. 2 – 1.8 1.14 0.12 2.2 4 MCV fl 91.51 – 2 .48 1 1.41 1 .55 105.8 – 04. 1 185. 14.14 1.2 5 MC pg 4.24 – 92. 1 5 .59 4.14 4 .59 – 4.11 59. .18 0. 4 MC C 24. – 45. 5.1 1. 2 2 . – 4 .1 4.1 1. 2 0.4 TLC 10 mm 00 14 5 11 2.1 49 .92 8500 1 540 12 4 . 55 .58 1. 2 8 TPC 10 mm 15800 44 5 0049.8 2182.4 1 8 48900 41 8.9 2525.89 1. 1 9 eterophils 50 9 5 2.0 50 8 0.8 2.41 1.24 10 Lymphocytes 1 8 2 . 1.81 21 42 .8 1.99 2. 11 Eosinophils 1 15 .2 0.92 2 4. 0.45 1.59 12 Monocytes 2 4 2.1 0. 2 0 4 1. 0.24 0.89 1 Basophils 0 0 0 0 0 0 0
Significant difference p 0.05 for each haematological parameters
Indian Journal of Biology / Volume 5 Number 1 / January June 2018 Sarbeswar Nayak & Prafulla Kumar Mohanty / aematological Analysis of Leschenault s Leaf Toad Gecko, 55 Hemidactylus Leschenaultii Dumeril and Bibron 18 parameters throughout the investigation. The total percentage of heterophils, eosinophils and monocytes erythrocyte count was found to be highest in male was highest in male and lowest in female Table 1 . and lowest in female p 0.05 . The percentage of The correlation between haematological parameters lymphocytes is highest in female and lowest in male of both male and female geckos was depicted p 0.05 . The highest mean value of haemoglobin and Figure 1 . There is a positive correlation between b packed cell volume and mean corpuscular vs PCV Fig.1a,b , B vs TEC Fig.1c,d and MCV vs haemoglobin concentration was found in male and MC Fig.1 k,l in both male and females, but B vs lowest in female while the mean values of other MCV Fig.1 e is positively correlated in male and parameters were found to be highest in female. The negatively correlated in female Fig.1 f . A negative
Indian Journal of Biology / Volume 5 Number 1 / January June 2018 5 Sarbeswar Nayak & Prafulla Kumar Mohanty / aematological Analysis of Leschenault s Leaf Toad Gecko, Hemidactylus Leschenaultii Dumeril and Bibron 18
ig. Correlation between different haematological parameters an of Hemidactylus leschenaultii Dumeril and Bibron 18 correlation was found in both se es with respect to higher in male of Free living Mediterranean Pond MCV vs TEC Fig.1g,h , MC vs TEC Fig.1 i,j and Turtle and TEC in other reptiles 9,24 . Our data MCV vs MC C Fig.1 m,n . regarding PCV and TEC corroborate with this. The PCV and TEC found in this study is lower than the report by 5 for prehensile tailed skink. The TLC Discussion was higher in male and MCV was higher in female Mediterranean Pond Turtle 4 . But in case of aematological data are essential to correlate the Leschenault s leaf toad gecko TLC and MCV was health status of reptiles with their habitat. found higher in female only. There was difference in aematology of reptiles provides a way to diagnose mean values of haematological parameters between the animals 1 . There was no differences in total se es of the studied gecko which is also seen in some haemoglobin concentration in male and female lizards agamidae 1 , geckos, Hemidactylus frenatus . Our 2, . owever the result of our investigation shows findings of TEC and TLC show closeness with the a difference in haemoglobin concentration. The finding of some agamidae lizards , 8, 9,40 . The difference in the value may be due to some seasonal result obtained in this study regarding the factors in combination with age and se of the haematological parameters also fall within the range individuals. According to 4 the PCV and TEC is as studied in iguana by 41,42 . aematological
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