ISSN:294­191 IndianJournalofBiology5 Volume 5Number 1,January ­June2018 OriginalArticle DOI:http://d.doi.org/10.21088/ijb.294.191.5118.8 aematologicalAnalysis oLeschenaultsLeaoadGecko, emidactlsescenaltiiDumerilandBibron SarbesarNayak, PraullaKumar Mohanty Abstract aematologyisusefulinunderstandingthephysiologicalfeaturesoflizards.Thestudywasheldto investigatethehaemoprofileofleschenaultsleaftoadgeckoinOdisha.Thehaematologicalparameters likeaemoglobinConcentrationB,PackedCellVolumePCV,TotalErythrocyteCountTEC,Mean CorpuscularVolumeMCV,MeanCorpuscularaemoglobinMC,MeanCorpuscularaemoglobin ConcentrationMCC,TotalLeucocyteCountTLC,TotalPlateletCountTPCandDifferentialLeucocyte CountDLCwerecalculatedusingstandardprocedures.Statisticalanalysislikecorrelationandt­test weredone.ThestudyrevealedthattheTECandpercentageoflymphocytesshowsignificantdifference p0.05betweenbothseesoflizard.Themeanvaluesofallotherparametersarealsoshowingdifference betweenmaleandfemaleofHemidactylusleschenaultiiDumerilandBibron18.Thecorrelationcoefficient variesinmaleandfemalewithrespecttotheparametersanalysed.Someparameterswerepositively correlatedwitheachotherandotherswerefoundtobenegativelycorrelated.Thedataobtainedcouldbe ausefulindicatorformonitoringandmanagingthegeneralhealthstatusofthespecies. KeyordsaematologicalParametersHemidactylusLeschenaultiiCorrelationSignificantDifference. Introduction AuthorsAiliation LecturerinZoologyOES­II, Department of Zoology, Vikram Deb Autonomous College, Jeypore, Koraput, Odisha 4001, India. Professor, PostgraduateDepartment ofZoology, UtkalUniversity, Vani Thesignificantofhaematologicalstudyfor Vihar, Bhubaneswar, Odisha51004, India. evaluating,analysing,eaminingandcontrollingthe healthstatusofanimalsisapreludeblueprint.The RerintsReuestSarbesarNayak, Lecturer in Zoology haematologictechniueforassessmentofbody OES­II, Department of Zoology, Vikram Deb Autonomous College, Jeypore, Koraput, Odisha 4001, India. physiology hasa great rolebeing uncomplicated, E­mail:nayaksarbeswar89gmail.com, serene,minimalinvasive,productivefirsthand prafulla.mohantygmail.com techniueandeconomicallysustainable. Received on29.01.2018, Accetedon 09.02.2018 Leschenaultsleaftoadgeckoisbelongingto Class–Reptilia,Order–SuamataandFamily cellhaemoglobinconcentrationwerestudiedinsome Gekkonidae,foundinwarmclimaticconditions reptiles10,11,12.aematologyinreptileshighly throughouttheworld1.aemocytological dependentonageaswellasseanditvaries parameters areusefulandwidelyusedtools that throughoutlife1,14.Thebloodcellswereidentified assist inthe diagnosis and monitoringof animal anddifferentparametersweretaken15­20. health 2. The combinations of different haematologicalparametersdetectthephysiological The data about haematologyof reptiles is still conditionsandclinicalevaluationinreptiles,4. challengingincomparisontoothernonmammalian Thedifferenteternalandinternalfactorsalsoaffect vertebrates.Theliteratureavailableonhaematology thehaemoprofileofnonmammalianvertebrates5,. of this lizard specifically from the studied aematolgyofsomesaurianswerestudied,8,9. geographicalregionofOdishaisveryless.The Thehaemoglobinconcentration,haematocritvalue, comparativescarcityofinformationsregardingthe meancellvolume,meancellhaemoglobinandmean haematologyofgeckosprovidesalessevidencesabout IndianJournalofBiology/Volume5Number1/January­June2018RedFlowerPublication Pvt.Ltd 54 SarbeswarNayak &Prafulla Kumar Mohanty /aematologicalAnalysis ofLeschenaultsLeafToadGecko, HemidactylusLeschenaultiiDumerilandBibron18 familyGekkonidae.Thepurposeofthisstudywasto andstainedwithGiemsaasprotocolcitedby determinethereferenceintervalsofhaematologyand Lillie2. morphometryofbloodcellsofnormalandapparently HaematologicalAnalysis healthylizards.Thefindingswillserveasbaseline dataforfuturehealthassessmentoflizardsaswell Thebloodparameterswerestudiedusingthe asforconservationandprotection. procedure24,25,2,2.Theconcentrationof haemoglobinwasestimatedasoyhaemoglobinby Sahalishaemometerandepresseding,Packed MaterialsandMethods cellvolumePCVwasdeterminedbymicrohaematocrit methodwithaspunofmicrohaematocrittubeat2500 rpmfor15minutes.TheuantificationofRBCsand Animals WBCswas performed by manualmethods using Fifteenlizardsofeachsewerecollectedfromthe haemocytometer, withayems dilutingfluid for coastalareaofRajnagarblockofKendrapara­54225, RBCsandTurksdilutingfluidforWBCs.Erythrocyte Odisha,locatedin2020Nto20Nlatitudeand indiceslikeMCV,MCandMCCwerecalculated 814Eto801Elongitude.Theywerecaughtatday using standard formulae 28. The percentage of timefromthecrevicesoftreesandsometimesatwallof differentleucocytesaswell astotalplateletcount boundariesandhomesadjacenttothefields.Lizards determined29,0. wereclinicallyhealthyandingoodconditiontransferred toanimalhouse.Theinvestigationofhaematological profileson HemidactylusleschenaultiiDumeril and StatisticalAnalysis Bibron18wascarriedoutfrom2014to201. ThedatapresentedasMeanSEStandardError forbothseesandMSofficeEcel200wasusedfor statisticalanalysis.Thecorrelationanalysisbetween BloodCollection theparametersandthesignificantdifferenceP0.05 Thevenipuncturesitewaspreparedaseptically wastaken usingStudents t­testassuming eual priortobloodcollection.Bloodwascollectedfrom varianceswiththehelpofPaleontologicalStatistics theventraltailveinoflizardsbyinsertinganinsulin PASTversion2.1NaturalistoryMuseum, syringeBDUltra–FineTMNeedle12.mm0Gat UniversityofOslo. anangle of 45­0 betweenthe scales on ventral midline21,22.Oncebloodappearedintheneedle hub,heldsteadyandagentlenegativepressurewas Results appliedtothesyringe.ThebloodwaskeptinanEDTA vialandthentransportedinicebotolaboratory.The aematologicalparameterswereanalysedincase lizardswerereleasedtotheirnaturalhabitatafter ofadultlizardTable1.Assumingaconfidencelevel collectionofblood.Wholebloodsmearwereobtained of95,significantdifferenceswere foundin two bypushslidetechniue,airdried,fiedwithmethanol ableaematological parametersof H.Leschenaultii Dumeril andBibron 18 Sl. Parameters nit Male emale P No. Range Mean SM Range Mean SM value 1 aemoglobin g 4.89–10.5 .49 0.41 4.5–9.4 .5 0. 1.5 2 PCV 11.–0. 21.25 1.4 11.89–0.89 20.12 1.9 0.54 TEC 10mm­ 0.8–2.4 1.4 0.14 0.2–1.8 1.14 0.12 2.2 4 MCV fl 91.51–2.48 11.41 1.55 105.8–04.1 185. 14.14 1.2 5 MC pg 4.24–92.1 5.59 4.14 4.59–4.11 59. .18 0.4 MCC 24.–45. 5.1 1.2 2.–4.1 4.1 1.2 0.4 TLC 10mm­ 00­145 112.1 49.92 8500­1540 124. 55.58 1.2 8 TPC 10mm­ 15800­445 0049.8 2182.4 18­48900 418.9 2525.89 1.1 9 eterophils 50­9 5 2.0 50­8 0.8 2.41 1.24 10 Lymphocytes 1­8 2. 1.81 21­42 .8 1.99 2. 11 Eosinophils 1­15 .2 0.92 2­ 4. 0.45 1.59 12 Monocytes 2­4 2.1 0.2 0­4 1. 0.24 0.89 1 Basophils 0 0 0 0 0 0 0 Significant difference p0.05for eachhaematological parameters IndianJournalofBiology/Volume5Number1/January­June2018 SarbeswarNayak &Prafulla Kumar Mohanty /aematologicalAnalysis ofLeschenaultsLeafToadGecko, 55 HemidactylusLeschenaultiiDumerilandBibron18 parametersthroughouttheinvestigation.Thetotal percentageofheterophils,eosinophilsandmonocytes erythrocytecountwasfoundtobehighestinmale washighestinmaleandlowestinfemaleTable1. and lowestin femalep0.05. Thepercentage of Thecorrelationbetweenhaematologicalparameters lymphocytesishighestinfemaleandlowestinmale ofbothmaleandfemalegeckoswasdepicted p0.05.Thehighestmeanvalueofhaemoglobinand Figure1.Thereisapositivecorrelationbetweenb packed cell volume and mean corpuscular vsPCVFig.1a,b,BvsTECFig.1c,dandMCVvs haemoglobinconcentrationwasfoundinmaleand MCFig.1k,linbothmaleandfemales,butBvs lowest in female while the mean valuesof other MCVFig.1eispositivelycorrelatedinmaleand parameterswerefoundtobehighestinfemale.The negativelycorrelatedinfemaleFig.1f.Anegative IndianJournalofBiology/Volume5Number1/January­June2018 5 SarbeswarNayak &Prafulla Kumar Mohanty /aematologicalAnalysis ofLeschenaultsLeafToadGecko, HemidactylusLeschenaultiiDumerilandBibron18 ig.Correlationbetween differenthaematological parameters a­nof Hemidactylus leschenaultiiDumeril andBibron 18 correlationwasfoundinbothseeswithrespectto higherinmaleofFreelivingMediterraneanPond MCVvsTECFig.1g,h,MCvsTECFig.1i,jand Turtleand TECin other reptiles 9,24.Our data MCVvsMCCFig.1m,n. regardingPCVandTECcorroboratewiththis.The PCVandTECfoundinthisstudyislowerthanthe reportby5forprehensiletailedskink.TheTLC Discussion washigherinmaleandMCVwashigherinfemale MediterraneanPondTurtle4.Butincaseof aematologicaldataareessentialtocorrelatethe LeschenaultsleaftoadgeckoTLCandMCVwas health status of reptiles with their habitat. foundhigherinfemaleonly.Therewasdifferencein aematologyofreptilesprovidesawaytodiagnose meanvaluesofhaematologicalparametersbetween theanimals1.Therewasnodifferencesintotal seesofthestudiedgeckowhichisalsoseeninsome haemoglobinconcentrationinmaleandfemalelizards agamidae1,geckos,Hemidactylusfrenatus.Our 2,.owevertheresultofourinvestigationshows findingsofTECandTLCshowclosenesswiththe adifferenceinhaemoglobinconcentration.The findingofsomeagamidaelizards,8,9,40.The differenceinthevaluemaybeduetosomeseasonal result obtained in this study regarding the factorsincombinationwithageandseofthe haematologicalparametersalsofallwithintherange individuals.Accordingto4thePCVandTECis asstudiediniguanaby41,42.aematological IndianJournalofBiology/Volume5Number1/January­June2018 SarbeswarNayak &Prafulla Kumar Mohanty /aematologicalAnalysis ofLeschenaultsLeafToadGecko, 5 HemidactylusLeschenaultiiDumerilandBibron18 parametersinreptilesvarywithageseand microhabitat and seasonal fluctuations on seasons1,4.TheTEC,inbothmaleandfemale haematologyoflizards. islowerincomparisonto Psammodromus