DOCSLIB.ORG

Pang Li Mei Thesis (Final) 21 May 2018.Pdf

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Pang Li Mei Thesis (Final) 21 May 2018.Pdf This document is downloaded from DR‑NTU (https://dr.ntu.edu.sg) Nanyang Technological University, Singapore. Bioprospecting indigenous actinomycetes for natural product discovery Pang, Li Mei 2018 Pang, L. M. (2018). Bioprospecting indigenous actinomycetes for natural product discovery. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/75687 https://doi.org/10.32657/10356/75687 Downloaded on 30 Sep 2021 11:26:30 SGT Bioprospecting Indigenous Actinomycetes for Natural Product Discovery Pang Li Mei SCHOOL OF BIOLOGICAL SCIENCES 2018 Bioprospecting Indigenous Actinomycetes for Natural Product Discovery Pang Li Mei SCHOOL OF BIOLOGICAL SCIENCES A thesis submitted to the Nanyang Technological University in partial fulfilment of the requirement for the degree of Doctor of Philosophy 2018 ii Acknowledgements First and foremost, I would like to express my gratitude to my supervisor, Assoc. Prof Liang Zhao-Xun for the opportunity to pursue a PhD programme in his laboratory. I am thankful for his patience, support and guidance throughout the past four years allowing me to experience different aspects of research life. His inspirational and motivational talks have brought me through the darkest moments of my research life. I would also like to thank my co-supervisor, Dr Tan Lik Tong (NIE) and thesis advisory committee, Prof James P Tam, Assoc. Prof Liu Chuan Fa and Assoc. Prof Newman Sze for their invaluable advice throughout my PhD. I am thankful for the research platform that NTU and MOE had provided for me to pursue my research. Next, I would like to thank National Parks Board for their assistance in our sample collection from Sungei Buloh Wetland Reserve. I would like to thank Assoc. Prof Cao Bin (CEE) and team for the providing samples from Pulau Ubin Quarry Lake. I would like to thank Asst. Prof Yang Liang (SCELSE) and his student Ding Yichen for the draft genome assembly of actinomycete strains. I would also like to thank our collaborators Dr Yoganathan K. (BII) and Assoc. Prof Valerie Lin for structure elucidation and estrogen response element assay for 4’,5-dihydroxy-7-methoxy-3-methylflavanone. Special thanks to Low Zhen Jie, Ye Hong and Gary Ding for providing the NMR spectra of 4’,5-dihydroxy- 7-methoxy-3-methylflavanone and echinomycin. This project would not be made possible without the help of a special team member, Low Zhen Jie, who fought extremely hard to get things to work. I am glad to be able to meet my fiancé during my PhD journey. His strengths complement my weaknesses which allowed me to pull through certain hurdles in experiments and in life. In addition, I am also grateful for the help and support from other lab members, Ye Rui Juan, Hoa Tran, Howard Saw, Xin Lingyi, Cheang Qing Wei, Sheng Shuo, and former FYP/attachment students. Lastly, I would like to thank my friends and families for their support and encouragement throughout my PhD programme. iii Table of contents Acknowledgements....................................................................................................... iii Table of contents ...........................................................................................................iv List of figures ................................................................................................................ vii List of tables ................................................................................................................... xi Abbreviations .............................................................................................................. xiii Abstract .......................................................................................................................... 1 CHAPTER 1: Background ........................................................................................... 3 1.1 Actinomycetes as a rich source of bioactive natural product .................... 3 1.2 Actinomycetes from unique environments ................................................. 5 1.3 Phenomenon of unculturable bacteria ........................................................ 7 1.4 Drug resistance and the need for new drug discovery ............................... 8 1.5 Discover bioactive compounds from actinomycetes in the post-genomics era ....................................................................................................................... 11 1.6 Overview of thesis ....................................................................................... 13 CHAPTER 2: Isolation of actinomycetes from mangrove and lake sediment ...... 15 2.1 Introduction ................................................................................................. 15 2.2 Materials and methods ............................................................................... 17 2.2.1 Source and sample collection ............................................................. 17 2.2.2 Pre-treatment methods ....................................................................... 19 2.2.3 Isolation media for actinomycetes ..................................................... 21 2.2.4 Assembly of in-situ cultivation disk ................................................... 25 2.2.5 Maintenance of strains ........................................................................ 26 2.2.6 Isolation of genomic DNA for 16S rDNA PCR amplification ......... 26 2.2.7 16S rDNA PCR amplification ............................................................ 27 2.2.8 Phylogenetic analysis .......................................................................... 27 2.3 Results .......................................................................................................... 28 2.3.1 Summary of strains from mangrove and lake sediment .................. 28 2.3.2 Comparison between direct plating and in-situ cultivation method ............................................................................................................... 31 2.3.3 Strain identification by 16S rDNA sequencing ................................. 32 2.4 Discussion ..................................................................................................... 36 2.5 Conclusion.................................................................................................... 39 CHAPTER 3: Strain prioritization by bioactivity screening .................................. 40 3.1 Introduction ................................................................................................. 40 iv 3.2 Materials and methods ............................................................................... 41 3.2.1 Cross streak assay ............................................................................... 41 3.2.2 Overlay assay ....................................................................................... 42 3.2.3 Fermentation of strains for bioactivity assays .................................. 43 3.2.4 Microtiter plate based antibacterial assay ........................................ 43 3.2.5 Microtiter plate based antifungal assay ............................................ 44 3.2.6 Anti-biofilm assay ............................................................................... 45 3.3 Results .......................................................................................................... 47 3.3.1 Antibacterial activities from Streptomyces sp. SW24, SD24 and SD50 ............................................................................................................... 48 3.3.2 Antibacterial activities from Streptomyces sp. P19 ........................... 49 3.3.3 Antibacterial and antifungal activities from Streptomyces sp. P9 ... 50 3.3.4 Antibacterial activities from Streptomyces sp. P46 ........................... 51 3.3.5 Antibacterial activities from Micromonospora sp. MD118 .............. 53 3.3.6 Antibacterial activities from Streptomyces sp. MD100 .................... 53 3.3.7 Antifungal activities from Streptomyces sp. MD102 and P7 ............ 54 3.3.8 Anti-biofilm activities from Streptomyces sp. SD9, SD35 and SD48 54 3.5 Conclusion.................................................................................................... 58 CHAPTER 4: Strain prioritization by genome sequencing and metabolite profiling ........................................................................................................................ 59 4.1 Introduction ................................................................................................. 59 4.2 Materials and methods ............................................................................... 60 4.2.1 Isolation of genomic DNA ................................................................... 60 4.2.2 Genome sequencing and assembly ..................................................... 61 4.2.3 Genome visualization of Micromonospora sp. MD118 with DNAplotter .......................................................................................................... 62 4.2.4 Analytical HPLC of crude extracts.................................................... 62 4.2.5 Fermentation of Streptomyces sp. P19 ............................................... 62 4.2.6 Fermentation of Streptomyces sp. SD50............................................. 63 4.3 Results .......................................................................................................... 63 4.3.1 Micromonospora sp. MD118 is a high potential strain that contains many novel biosynthetic gene
Load more

Recommended publications
  • Protective Effects of Berberis Crataegina DC. (Ranunculales: Berberidaceae) Extract on Bleomycin-Induced Toxicity in Fruit Flies (Diptera: Drosophilidae)
    Revista de la Sociedad Entomológica Argentina ISSN: 0373-5680 ISSN: 1851-7471 [email protected] Sociedad Entomológica Argentina Argentina Protective effects of Berberis crataegina DC. (Ranunculales: Berberidaceae) extract on Bleomycin-induced toxicity in fruit flies (Diptera: Drosophilidae) ATICI, Tuğba; ALTUN ÇOLAK, Deniz; ERSÖZ, Çağla Protective effects of Berberis crataegina DC. (Ranunculales: Berberidaceae) extract on Bleomycin-induced toxicity in fruit flies (Diptera: Drosophilidae) Revista de la Sociedad Entomológica Argentina, vol. 77, no. 4, 2018 Sociedad Entomológica Argentina, Argentina Available in: https://www.redalyc.org/articulo.oa?id=322055706003 PDF generated from XML JATS4R by Redalyc Project academic non-profit, developed under the open access initiative Artículos Protective effects of Berberis crataegina DC. (Ranunculales: Berberidaceae) extract on Bleomycin-induced toxicity in fruit flies (Diptera: Drosophilidae) Efectos protectores de extracto de Berberis crataegina DC. (Ranunculales: Berberidaceae) frente a toxicidad inducida con Bleomicina en moscas de la fruta (Diptera: Drosophilidae) Tuğba ATICI Institute of Natural and Applied Science, Erzincan Binali Yıldırım University, Turquía Deniz ALTUN ÇOLAK [email protected] Faculty of Art and Science, Erzincan Binali Yıldırım University, Turquía Çağla ERSÖZ Revista de la Sociedad Entomológica Institute of Natural and Applied Science, Erzincan Binali Yıldırım Argentina, vol. 77, no. 4, 2018 University, Turquía Sociedad Entomológica Argentina, Argentina Received: 02 October 2018 Accepted: 06 December 2018 Published: 27 December 2018 Abstract: Antineoplastic agents due to their cytotoxic effects increase the amount of free radical in cells causing cell death. e aims of this study were to assess the possible Redalyc: https://www.redalyc.org/ toxic effects of Bleomycin (BLM) on the number of offspring and survival rate of fruit articulo.oa?id=322055706003 flies as well as the protective role of Berberis crataegina DC.
    [Show full text]
  • Colonization of Lettuce Rhizosphere and Roots by Tagged Streptomyces
    PhD School of Chemistry, Biochemistry and Ecology of Pesticide (CBEA) DOCTORAL DISSERTATION Tagging biocontrol Streptomyces to study lettuce colonization Xiaoyulong Chen To be presented with the permission of the examining board, 28th cycle of the CBEA PhD school, for public examination at Aula Magna C03 (Room C03), Faculty of Agricultural and food sciences, University of Milan, on 17.12.2015. Cover figure: Colonization of lettuce root by Streptomyces cyaneus ZEA17I, examined by confocal laser scanning microscopy. Supervisor: Professor Paolo Cortesi Department of Food, Environmental, and Nutritional Sciences University of Milan, Italy Co-supervisor: Professor Marco Saracchi Department of Food, Environmental, and Nutritional Sciences University of Milan, Italy Coordinator: Professor Daniele Daffonchio Department of Food, Environmental, and Nutritional Sciences University of Milan, Italy Examination board (ordered by first letter of the family name): 1. Professor Paolo Bertolini Department of Agricultural Sciences University of Bologna, Italy 2. Dr. Jonas Bengtsson Department of Zoology Stockholm University, Sweden 3. Professor Daniele Daffonchio Department of Food, Environmental, and Nutritional Sciences University of Milan, Italy 4. Professor George Tsiamis Dep. of Environmental and Nutritional Resources Management University of Patras, Greece 5. Dr. Laura Polito Institute of Molecular Science and Technology, CNR, Italy Contents List of original publications......................................................................................................
    [Show full text]
  • Actinobacteria and Myxobacteria Isolated from Freshwater Snails and Other Uncommon Iranian Habitats, Their Taxonomy and Secondary Metabolism
    Actinobacteria and Myxobacteria isolated from freshwater snails and other uncommon Iranian habitats, their taxonomy and secondary metabolism Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades einer Doktorin der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Nasim Safaei aus Teheran / Iran 1. Referent: Professor Dr. Michael Steinert 2. Referent: Privatdozent Dr. Joachim M. Wink eingereicht am: 24.02.2021 mündliche Prüfung (Disputation) am: 20.04.2021 Druckjahr 2021 Vorveröffentlichungen der Dissertation Teilergebnisse aus dieser Arbeit wurden mit Genehmigung der Fakultät für Lebenswissenschaften, vertreten durch den Mentor der Arbeit, in folgenden Beiträgen vorab veröffentlicht: Publikationen Safaei, N. Mast, Y. Steinert, M. Huber, K. Bunk, B. Wink, J. (2020). Angucycline-like aromatic polyketide from a novel Streptomyces species reveals freshwater snail Physa acuta as underexplored reservoir for antibiotic-producing actinomycetes. J Antibiotics. DOI: 10.3390/ antibiotics10010022 Safaei, N. Nouioui, I. Mast, Y. Zaburannyi, N. Rohde, M. Schumann, P. Müller, R. Wink.J (2021) Kibdelosporangium persicum sp. nov., a new member of the Actinomycetes from a hot desert in Iran. Int J Syst Evol Microbiol (IJSEM). DOI: 10.1099/ijsem.0.004625 Tagungsbeiträge Actinobacteria and myxobacteria isolated from freshwater snails (Talk in 11th Annual Retreat, HZI, 2020) Posterbeiträge Myxobacteria and Actinomycetes isolated from freshwater snails and
    [Show full text]
  • The Rising of “Modern Actinobacteria” Era Jodi Woan-Fei Law1, Vengadesh Letchumanan1, Loh Teng-Hern Tan1, Hooi-Leng Ser1, Bey-Hing Goh2, Learn-Han Lee1*
    Review Article Progress in Microbes and Molecular Biology The Rising of “Modern Actinobacteria” Era Jodi Woan-Fei Law1, Vengadesh Letchumanan1, Loh Teng-Hern Tan1, Hooi-Leng Ser1, Bey-Hing Goh2, Learn-Han Lee1* 1Novel Bacteria and Drug Discovery Research Group (NBDD), Microbiome and Bioresource Research Strength (MBRS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 47500 Bandar Sunway, Selangor Darul Ehsan, Malaysia. 2Biofunctional Molecule Exploratory Research Group (BMEX), School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Selangor Darul Ehsan, Malaysia. Abstract: The term “Modern Actinobacteria” (MOD-ACTINO) was coined by a Malaysian Scientist Dr. Lee Learn-Han, who has great expertise and experience in the field of actinobacteria research. MOD-ACTINO is defined as a group of actinobacteria capable of producing compounds that can be explored for modern applications such as development of new drugs and cosmeceutics. MOD-ACTINO members consist of already identified or novel actinobacteria isolated from special environments: mangrove, desert, lake, hot spring, cave, mountain, Arctic and Antarctic regions. These actinobac- teria are valuable sources for various industries which can contribute directly/indirectly towards the improvement in many aspects of our lives. Keywords: modern; bioactive; actinobacteria; *Correspondence: Learn-Han Lee, Novel Bacteria and environment; bioprospecting Drug Discovery Research Group (NBDD), Microbiome and Biore-source Research Strength (MBRS), Jeffrey Cheah School of Medicine and Health Sciences, Monash Uni- Received: 16th February 2020 versity Malaysia, 47500 Bandar Sunway, Selangor Darul Accepted: 23rd March 2020 Ehsan, Malaysia. lee. [email protected] Published Online: 29th March 2020 Citation: Law JW-F, Letchumanan V, Tan LT-H et al.
    [Show full text]
  • Biosynthesis and Engineering of Cyclomarin and Cyclomarazine: Prenylated, Non-Ribosomal Cyclic Peptides of Marine Actinobacterial Origin
    UC San Diego Research Theses and Dissertations Title Biosynthesis and Engineering of Cyclomarin and Cyclomarazine: Prenylated, Non-Ribosomal Cyclic Peptides of Marine Actinobacterial Origin Permalink https://escholarship.org/uc/item/21b965z8 Author Schultz, Andrew W. Publication Date 2010 Peer reviewed eScholarship.org Powered by the California Digital Library University of California UNIVERSITY OF CALIFORNIA, SAN DIEGO Biosynthesis and Engineering of Cyclomarin and Cyclomarazine: Prenylated, Non-Ribosomal Cyclic Peptides of Marine Actinobacterial Origin A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Oceanography by Andrew William Schultz Committee in charge: Professor Bradley Moore, Chair Professor Eric Allen Professor Pieter Dorrestein Professor William Fenical Professor William Gerwick 2010 Copyright Andrew William Schultz, 2010 All rights reserved. The Dissertation of Andrew William Schultz is approved, and it is acceptable in quality and form for publication on microfilm and electronically: ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ Chair University of California, San Diego 2010 iii DEDICATION To my wife Elizabeth and our son Orion and To my parents Dale and Mary Thank you for your never ending love and support iv TABLE OF CONTENTS Signature Page ....................................................................................................
    [Show full text]
  • Enhancement of Bleomycin Production in Streptomyces Verticillus Through Global Metabolic Regulation of N-Acetylglucosamine and A
    Chen et al. Microb Cell Fact (2020) 19:32 https://doi.org/10.1186/s12934-020-01301-8 Microbial Cell Factories RESEARCH Open Access Enhancement of bleomycin production in Streptomyces verticillus through global metabolic regulation of N-acetylglucosamine and assisted metabolic profling analysis Hong Chen1,2†, Jiaqi Cui1,2†, Pan Wang1,2, Xin Wang1,2 and Jianping Wen1,2* Abstract Background: Bleomycin is a broad-spectrum glycopeptide antitumor antibiotic produced by Streptomyces verticil- lus. Clinically, the mixture of bleomycin A2 and bleomycin B2 is widely used in combination with other drugs for the treatment of various cancers. As a secondary metabolite, the biosynthesis of bleomycin is precisely controlled by the complex extra-/intracellular regulation mechanisms, it is imperative to investigate the global metabolic and regula- tory system involved in bleomycin biosynthesis for increasing bleomycin production. Results: N-acetylglucosamine (GlcNAc), the vital signaling molecule controlling the onset of development and antibiotic synthesis in Streptomyces, was found to increase the yields of bleomycins signifcantly in chemically defned medium. To mine the gene information relevant to GlcNAc metabolism, the DNA sequences of dasR-dasA-dasBCD- nagB and nagKA in S. verticillus were determined by chromosome walking. From the results of Real time fuorescence quantitative PCR (RT-qPCR) and electrophoretic mobility shift assays (EMSAs), the repression of the expression of nagB and nagKA by the global regulator DasR was released under induction with GlcNAc. The relief of blmT expres- sion repression by BlmR was the main reason for increased bleomycin production. DasR, however, could not directly afect the expression of the pathway-specifc repressor BlmR in the bleomycins gene cluster.
    [Show full text]
  • Marine Rare Actinomycetes: a Promising Source of Structurally Diverse and Unique Novel Natural Products
    Review Marine Rare Actinomycetes: A Promising Source of Structurally Diverse and Unique Novel Natural Products Ramesh Subramani 1 and Detmer Sipkema 2,* 1 School of Biological and Chemical Sciences, Faculty of Science, Technology & Environment, The University of the South Pacific, Laucala Campus, Private Mail Bag, Suva, Republic of Fiji; [email protected] 2 Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands * Correspondence: [email protected]; Tel.: +31-317-483113 Received: 7 March 2019; Accepted: 23 April 2019; Published: 26 April 2019 Abstract: Rare actinomycetes are prolific in the marine environment; however, knowledge about their diversity, distribution and biochemistry is limited. Marine rare actinomycetes represent a rather untapped source of chemically diverse secondary metabolites and novel bioactive compounds. In this review, we aim to summarize the present knowledge on the isolation, diversity, distribution and natural product discovery of marine rare actinomycetes reported from mid-2013 to 2017. A total of 97 new species, representing 9 novel genera and belonging to 27 families of marine rare actinomycetes have been reported, with the highest numbers of novel isolates from the families Pseudonocardiaceae, Demequinaceae, Micromonosporaceae and Nocardioidaceae. Additionally, this study reviewed 167 new bioactive compounds produced by 58 different rare actinomycete species representing 24 genera. Most of the compounds produced by the marine rare actinomycetes present antibacterial, antifungal, antiparasitic, anticancer or antimalarial activities. The highest numbers of natural products were derived from the genera Nocardiopsis, Micromonospora, Salinispora and Pseudonocardia. Members of the genus Micromonospora were revealed to be the richest source of chemically diverse and unique bioactive natural products.
    [Show full text]
  • Exploring the Potential of Antibiotic Production from Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis
    fmicb-11-01540 July 27, 2020 Time: 14:51 # 1 ORIGINAL RESEARCH published: 15 July 2020 doi: 10.3389/fmicb.2020.01540 Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis Dini Hu1,2, Chenghang Sun3, Tao Jin4, Guangyi Fan4, Kai Meng Mok2, Kai Li1* and Simon Ming-Yuen Lee5* 1 School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China, 2 Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Macau, China, 3 Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, 4 Beijing Genomics Institute, Shenzhen, China, 5 State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China Actinobacteria are well recognized for their production of structurally diverse bioactive Edited by: secondary metabolites, but the rare actinobacterial genera have been underexploited Sukhwan Yoon, for such potential. To search for new sources of active compounds, an experiment Korea Advanced Institute of Science combining genomic analysis and tandem mass spectrometry (MS/MS) screening and Technology, South Korea was designed to isolate and characterize actinobacterial strains from a mangrove Reviewed by: Hui Li, environment in Macau. Fourteen actinobacterial strains were isolated from the collected Jinan University, China samples. Partial 16S sequences indicated that they were from six genera, including Baogang Zhang, China University of Geosciences, Brevibacterium, Curtobacterium, Kineococcus, Micromonospora, Mycobacterium, and China Streptomyces. The isolate sp.01 showing 99.28% sequence similarity with a reference *Correspondence: rare actinobacterial species Micromonospora aurantiaca ATCC 27029T was selected for Kai Li whole genome sequencing.
    [Show full text]
  • Polyphasic Systematics of Marine Bacteria and Their Alpha-Glucosidase Inhibitor Activity
    Polyphasic systematics of marine bacteria and their alpha-glucosidase inhibitor activity Thesis Submitted to AcSIR For the Award of the Degree of DOCTOR OF PHILOSOPHY In Biological Science By RAHUL BHOLESHANKAR MAWLANKAR AcSIR no. 10BB13J26036 Under the guidance of Research Supervisor Dr. Syed G. Dastager Research Co-supervisor Dr. Mahesh S. Dharne NCIM Resource Centre, Biochemical Science divison, CSIR-National Chemical Laboratory, Pune-411 008, India Table of contents Table of contents 1 Certificate 4 Declaration 5 Acknowledgment 6 List of fugures 9 List of tables 12 List of abbreviations 14 Abstract 16 Chapter 1. Introduction 19 1.1. Bacterial Systematics 20 1.1.1. Phenotypic analysis 22 1.1.2. Phylogenetic analysis 28 1.1.2.1. The 16S rRNA gene sequencing 29 1.1.2.2. Phylogenetic analysis 30 1.1.2.3. Whole genome analysis 32 1.1.3. Genotypic analysis 33 1.1.3.1. DNA-DNA hybridization (DDH) 33 1.1.3.2. Genomic DNA G+C content 35 1.1.3.3. Multi-locus sequence typing (MLST) 36 1.1.3.4. DNA profiling 37 1.2. Marine bacteria and their potentials 38 1.3. Marine sediments 39 1.4. Alpha-glucosidase inhibitor 44 1.4.1. Acarbose 45 1.4.2. Voglibose 48 1.4.3. Nojirimycin 49 1.4.4. 1-deoxynojirimycin 50 1.4.5. Miglitol 51 1.5. Alpha-glucosidase inhibitors from marine isolates 52 Chapter 2. Polyphasic Systematic approach 55 2.1. Overview 56 2.2. Isolation of marine sediment sample 57 2.3. Characterization 57 2.3.1.
    [Show full text]
  • Streptomyces Monashensis Sp. Nov., a Novel Mangrove Soil
    www.nature.com/scientificreports Corrected: Author Correction OPEN Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Received: 13 September 2018 Accepted: 21 January 2019 Malaysia with antioxidative Published online: 28 February 2019 potential Jodi Woan-Fei Law1, Hooi-Leng Ser1,2,3, Nurul-Syakima Ab Mutalib 4, Surasak Saokaew 1,5,6, Acharaporn Duangjai1,5,7, Tahir Mehmood Khan2,8, Kok-Gan Chan 9,10, Bey-Hing Goh2,3,5 & Learn-Han Lee1,3,5 A new Streptomyces species discovered from Sarawak mangrove soil is described, with the proposed name – Streptomyces monashensis sp. nov. (strain MUSC 1JT). Taxonomy status of MUSC 1JT was determined via polyphasic approach. Phylogenetic and chemotaxonomic properties of strain MUSC 1JT were in accordance with those known for genus Streptomyces. Based on phylogenetic analyses, the strains closely related to MUSC 1JT were Streptomyces corchorusii DSM 40340T (98.7%), Streptomyces olivaceoviridis NBRC 13066T (98.7%), Streptomyces canarius NBRC 13431T (98.6%) and Streptomyces coacervatus AS-0823T (98.4%). Outcomes of DNA–DNA relatedness between strain MUSC 1JT and its closely related type strains covered from 19.7 ± 2.8% to 49.1 ± 4.3%. Strain MUSC 1JT has genome size of 10,254,857 bp with DNA G + C content of 71 mol%. MUSC 1JT extract exhibited strong antioxidative activity up to 83.80 ± 4.80% in the SOD assay, with signifcant cytotoxic efect against colon cancer cell lines HCT-116 and SW480. Streptomyces monashensis MUSC 1JT (=DSM 103626T = MCCC 1K03219T) could potentially be a producer of novel bioactive metabolites; hence discovery of this new species may be highly signifcant to the biopharmaceutical industry as it could lead to development of new and useful chemo-preventive drugs.
    [Show full text]
  • Towards Combinatorial Biosynthesis of Pyrrolamide Antibiotics in Streptomyces Celine Aubry
    Towards combinatorial biosynthesis of pyrrolamide antibiotics in Streptomyces Celine Aubry To cite this version: Celine Aubry. Towards combinatorial biosynthesis of pyrrolamide antibiotics in Streptomyces. Bio- chemistry [q-bio.BM]. Université Paris Saclay (COmUE), 2019. English. NNT : 2019SACLS245. tel-02955510 HAL Id: tel-02955510 https://tel.archives-ouvertes.fr/tel-02955510 Submitted on 2 Oct 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Towards combinatorial biosynthesis of pyrrolamide antibiotics in Streptomyces Thèse de doctorat de l'Université Paris-Saclay préparée à l’Université Paris-Sud École doctorale n°577 Structure et Dynamique des Systèmes Vivants (SDSV) Spécialité de doctorat : Sciences de la vie et de la Santé Thèse présentée et soutenue à Orsay, le 30/09/19, par Céline AUBRY Composition du Jury : Matthieu Jules Professeur, Agroparistech (MICALIS) Président du Jury Yanyan Li Chargée de recherche, MNHN (MCAM) Rapportrice Stéphane Cociancich Chercheur, CIRAD (BGPI) Rapporteur Annick Méjean Professeure, Université Paris-Diderot (LIED) Examinatrice Hasna Boubakri Maitre de conférences, Université Claude Bernard Lyon I (Ecologie microbienne) Examinatrice Sylvie Lautru Chargée de recherche, CNRS (I2BC) Directrice de thèse Acknowledgements J’ai insisté pour rédiger l’ensemble de ma thèse en anglais.
    [Show full text]
  • Exploration of Chemical Interactions Between Streptomyces and Eukaryotes
    i Exploration of chemical interactions between Streptomyces and eukaryotes By Louis K. Ho A thesis submitted in conformity with the requirements For the degree of Doctor of Philosophy Department of Biochemistry University of Toronto © Copyright by Louis K. Ho 2020 i Exploration of chemical interactions between Streptomyces and eukaryotes Louis K. Ho Doctor of Philosophy Department of Biochemistry University of Toronto 2020 Abstract In the environment, bacteria live in complex communities with other organisms. In this work, I characterize two new chemically-mediated ways in which bacteria and eukaryotes interact. First, I show that ingestion of Streptomyces bacteria can directly be lethal to fruit flies and that this toxicity is the result of bacterial production of insecticides. I also show that this toxicity is facilitated by airborne odors that reducesin insect progeny. Second, I show that the yeast Saccaromyces cerevisiae can trigger Streptomyces to enter a new mode of growth called ‘exploration’. I characterize ‘exploratory’ growth and identify how it is triggered by airborne chemicals and the chemical modification of the growth environment. Overall, this thesis describes two new ways in which bacteria and eukaryotes interact and highlights how chemicals play an important role in these interactions. ii ii Acknowledgements I would like to thank all past and present members of the laboratory for their support and putting up with me throughout the years. You are not only my trusted colleagues but also my friends that I hope to cherish and keep in touch with for many years to come*. I would like to thank my supervisor, Dr. Justin Nodwell for putting insurmountable faith in me from the very beginning and for shaping my view of the world.
    [Show full text]