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îNFORMÂTiüN i?J ilsHiUS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. University Microfilms international A Bell & Howell Information Company 300 Nortti Zeeb Road. Ann Arbor. Ml 48106-1346 USA 313/761-4700 800/521-0600 Order Number 9201729 Comparative ultrastructure of fossil gyrtmosperm pollen and implications regarding the origin of angiosperms Osborn, Jeffrey Mark, Ph.D. The Ohio State University, 1991 U’M'I 300 N. Zeeb Rd. Ann Arbor, MI 48106 COMPARATIVE ULTRASTRUCTURE OF FOSSIL GYMNOSPERM POLLEN AND IMPLICATIONS REGARDING THE ORIGIN OF ANGIOSPERMS DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Jeffrey Mark Osborn, B. S., M. S. ***** The Ohio State University 1991 Dissertation Committee: Approved by Thomas N. Taylor Valayamghat Raghavan Fred D. Sack \ Adviser Edith L. Taylor Department of Plant Biology To Dr. Alfred S. Osborn, Sr. ACKNOWLEDGEMENTS This research was financially supported by several institutions and agencies. These include: the National Science Foundation (Dissertation Improvement Award; BSR- 9016397), the American Association of Stratigraphie Palynologists (Student Scholarship), Sigma-Xi, The Scientinc Research Society (Grant-in-aid of Research), the Graduate School, The Ohio State University (Graduate Student Alumni Research Award and Presidential Fellowship), and the Department of Plant Biology, The Ohio State University (Graduate Teaching and Research Associateships). The outcome of this investigation was significantly aided by their assistance and it is gratefully acknowledged. Numerous individuals have also contributed in variety of ways to both this investigation as well as my other scholarly endeavors. I would like to thank the paleobotany group (Dr. L. J. Hickey, Dr. D. W. Taylor, L. Klise, and A. Muller) at the Peabody Museum of Natural History, Yale University for providing much assistance and a generous loan of Cycadeoidea material. Several members of both the Paleobotany and Botany Departments (Drs. F. M. Hueber, S. H. Mamay, W. A. DiMichele, S. L. Wing, and R. A. Faden), National Museum of Natural History, Smithsonian Institution were also especially helpful during a my visit there. I am also grateful to Dr. T. Rich and the staff of the National Museum of Victoria for loaning the specimen of Sahnia laxiphora, and Dr. M. R. de Lima for providing Cretaceous sediments fi*om the Santana Formation. I am especially appreciative of the following individuals, who gave of their time in order to write supporting letters of my various grant, fellowship, and employment proposals/applications: Drs. T. N. Taylor, T. Delevoryas, E. L. Schneider, J. R. Rowley, ni G. Piayford, D. L. Diicher, R. A. Stockey, W. L. Crepet, E. L. Taylor, V. Raghavan, D. J. Crawford, and R. E. J. Boemer. î would also like to acknowledge the assistance of Dr. W. A. Taylor, who was helpful in the early stages of this research in sharing his technical expertise on electron microscopy. Drs. E. L. Taylor, F. D. Sack, and V. Raghavan deserve much recognition and thanks. They graciously donated significant amounts of their time and effort by serving on my Advisory, General Examination, and Dissertation Reading Committees, and further contributed by providing helpful comments on this manuscript. Î am especially grateful to Dr. T. N. Taylor for the role he has played as my adviser. He has provided unrelenting guidance and encouragement at both professional and personal levels. As a student I have learned a great deal from him, and I am quite certain that as a colleague, he will remain to be a source of much knowledge and inspiration for me. Finally, I am deeply indebted to my wife, Yolanda, as well as my family for their loving support, without which this investigation would not have been possible. Yolanda has been especially understanding during the final months of preparation of this document, and I love her dearly for it IV VTTA 29 November 1963 ........................................ ...... Bora - Houston, Texas 1982-1985 ............................................................... Undergraduate Laboratory Instructor, Undergraduate Research Assistant, Southwest Texas State University, San Marcos, Texas 1985 ......................................................................... B. S. in Marine Biology, Southwest Texas State University, graduated cum laude 1985-1986 ............................................................... Graduate Laboratoiy Instructor, Graduate Research Assistant, Southwest Texas State University 1986-1987 ............................................................... Assistant Instructor, Southwest Texas State University 1987 ......................................................................... M. S. in Botany, Southwest Texas State University 1987-1990 ............................................................... Graduate Teaching Associate, Graduate Research Associate, The Ohio State University, Columbus, Ohio 1990-1991 ............................................................... Presidential Fellow, The Ohio State University 1991- .................................................................... Assistant Professor, Northeast Missouri State University, Kirksville, Missouri 1992 ......................................................................... International Fellow, Department of Botany, University of Alberta, Edmonton, Alberta, Canada PUBLICATIONS (Refereed Papers) Osborn, J. M. and E. L. Schneider. 1988. Morphological studies of the Nympheaeceae sensu iato. XVI. The floral biology of Brasenia schreberi. Annals of the Missouri Botanical Garden 75:778-794. Osborn, J. M., T. N. Taylor, and J. F. White, Jr. 1989. Falaeofibulus, gen. nov., a clamp-bearing fungus from the Triassic of Antarcdca. Mycologia 81: 622-626. Osborn, J. M. and T. N. Taylor. 1989. Structurally preserved sphenophytes from the Triassic of Antarctica: Vegetative remains of Spaciinodum, gen. nov. American Journal of Botany 16:1594-1601. Osborn, J. M. and T. N. Taylor. 1990. Morphological and ultrastructural studies of plant cuticular membranes. I. Sun and shade leaves of Quercus velutina (Fagaceae). Botanical Gazette 151:465-476. Taylor, T. N. and J. M. Osborn. 1991. The role of wood in understanding saprophytism in the fossil record. CFS-Courier Forschungsinstitut Senckenberg. (in press) Osborn, J. M., T. N. Taylor, and E. L. Schneider. 1991. Pollen morphology and ultrastructure of the Cabombaceae: Correlations with pollination biology. American Journal of Botany 78(9). (in press) Osborn, J. M., T. N. Taylor, and P. R. Crane. 1991. The ultrastructure of Sahnia pollen (Pentoxylales). American Journal of Botany, (in press) (Abstracts) Osbom, J. M. and E. L. Schneider. 1987. The reproductive biology of Brasenia schreberi J. F. Gmelin (Nymphaeaceae). Texas Academy of Science, March Program Abstr. 90: 49. Osbom, J. M. and E. L. Schneider. 1987. Wind pollination in Brasenia schreberi (Nymohaeaceae). Southwestern Association of Naturalists, April Program Abstr. 1987:72 Osbom, J. M. and E. L. Schneider. 1987. Morphological studies of the Nymphaeaceae. XVI. The floral biology of Brasenia schreberi J. F. Gmelin. IBC-87, XIV Intemational Botanic^ Congress, Berlin (West), Germany. Abstracts, 4-20-7: 224. Osbom, J. M. and E. L, Schneider. 1987. Anemophily in Brasenia (Nymphaeaceae). American Journal of Botany 74 (5): 622-623. Schneider, E. L. and J. M. Osbom. 1987. Morphological studies of the Nymphaeaceae. American Journal of Botany 74 (5): 721. VI Osbom, J. M. and T. N. Tayior. 1988. Mesozoic sphenophytes from Antarctica. American Journal of Botany 75 (6, supplement): 113. Osbom, J. M. and T. N. Taylor. 1989. Cuticle ultrastmcture and micromorphology from sun and shade leaves of Quercus velutina Lam. ^lack Oak). Ohio Journal of Science (April Program Abst.) 1989: 7. Osbom, J. M., T. N. Taylor, and J. F. White, Jr. 1989. A fossil fungus possessing clamp connections from the Triassic of Antarctica. Ohio Journal of Science (April Program Abst.) 1989: 7. Osbom, J. M. and T. N. Taylor. 1989. Morphological and ultrastructural studies of plant cuticle: Sun and shade leaves of Quercus velutina Lam. American Journal of Botany 76 (6, supplement): 50.