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Systematic Entomology (2017), 42, 60–81 DOI: 10.1111/syen.12210 A molecular phylogeny and revised higher-level classification for the leaf-mining moth family Gracillariidae and its implications for larval host-use evolution AKITO Y. KAWAHARA1, DAVID PLOTKIN1,2, ISSEI OHSHIMA3, CARLOS LOPEZ-VAAMONDE4,5, PETER R. HOULIHAN1,6, JESSE W. BREINHOLT1, ATSUSHI KAWAKITA7, LEI XIAO1,JEROMEC. REGIER8,9, DONALD R. DAVIS10, TOSIO KUMATA11, JAE-CHEON SOHN9,10,12, JURATE DE PRINS13 andCHARLES MITTER9 1Florida Museum of Natural History, University of Florida, Gainesville, FL, U.S.A., 2Department of Entomology and Nematology, University of Florida, Gainesville, FL, U.S.A., 3Kyoto Prefectural University, Kyoto, Japan, 4INRA, UR0633 Zoologie Forestière, Orléans, France, 5IRBI, UMR 7261, CNRS/Université François-Rabelais de Tours, Tours, France, 6Department of Biology, University of Florida, Gainesville, FL, U.S.A., 7Center for Ecological Research, Kyoto University, Kyoto, Japan, 8Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, U.S.A., 9Department of Entomology, University of Maryland, College Park, MD, U.S.A., 10Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington, DC, U.S.A., 11Hokkaido University Museum, Sapporo, Japan, 12Department of Environmental Education, Mokpo National University, Muan, South Korea and 13Department of Entomology, Royal Belgian Institute of Natural Sciences, Brussels, Belgium Abstract. Gracillariidae are one of the most diverse families of internally feeding insects, and many species are economically important. Study of this family has been hampered by lack of a robust and comprehensive phylogeny. In the present paper, we sequenced up to 22 genes in 96 gracillariid species, representing all previously rec- ognized subfamilies and genus groups, plus 20 outgroups representing other families and superfamilies.
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