NFKBIE Polyclonal Antibody

NFKBIE polyclonal antibody Gene Summary: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM Catalog Number: PAB18097 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B Regulatory Status: For research use only (RUO) proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in Product Description: Rabbit polyclonal antibody raised the cytoplasm. Phosphorylation of serine residues on the against synthetic peptide of NFKBIE. I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the Immunogen: A synthetic peptide corresponding to ubiquitination pathway, thereby allowing activation of the human NFKBIE. NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at Host: Rabbit kappa-B-binding motifs such as 5-prime GGGRNNYYCC Reactivity: Human,Mouse,Rat 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T Applications: ELISA, IF, IHC-P, WB-Ce pyrimidine). For some genes, activation requires NFKB (See our web site product page for detailed applications interaction with other transcription factors, such as STAT information) (see STAT6, MIM 601512), AP1 (JUN, MIM 165160), and NFAT (see NFATC1, MIM 600489).[supplied by Protocols: See our web site at OMIM] http://www.abnova.com/support/protocols.asp or product page for detailed protocols References: 1. Identification of NAP1, a regulatory subunit of IkappaB Specificity: This antibody is specific to NFKBIE. kinase-related kinases that potentiates NF-kappaB signaling. Fujita F, Taniguchi Y, Kato T, Narita Y, Furuya Form: Liquid A, Ogawa T, Sakurai H, Joh T, Itoh M, Delhase M, Karin M, Nakanishi M. Mol Cell Biol. 2003 Purification: Affinity purification Nov;23(21):7780-93. 2. NFkappaB and caspase-3 activity in apoptotic Concentration: 1 mg/mL hepatocytes of galactosamine-sensitized mice treated Recommend Usage: Western Blot (1:500-1:1000) with TNFalpha. Tapalaga D, Tiegs G, Angermuller S. J Immunohistochemistry (1:50-1:100) Histochem Cytochem. 2002 Dec;50(12):1599-609. Immunofluorescence (1:500-1:1000) 3. Interaction between hnRNPA1 and IkappaBalpha is ELISA (1:40000) required for maximal activation of NF-kappaB-dependent The optimal working dilution should be determined by transcription. Hay DC, Kemp GD, Dargemont C, Hay RT. the end user. Mol Cell Biol. 2001 May;21(10):3482-90.

Storage Buffer: In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)

Storage Instruction: Store at -20°C. Aliquot to avoid repeated freezing and thawing.

Entrez GeneID: 4794

Gene Symbol: NFKBIE

Gene Alias: IKBE

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