VECTOR-BORNE AND ZOONOTIC DISEASES Volume 15, Number 6, 2015 ORIGINAL ARTICLES ª Mary Ann Liebert, Inc. DOI: 10.1089/vbz.2014.1699

Orbiviruses: A North American Perspective

D. Scott McVey,1 Barbara S. Drolet,1 Mark G. Ruder,1 William C. Wilson,1 Dana Nayduch,1 Robert Pfannenstiel,1 Lee W. Cohnstaedt,1 N. James MacLachlan,2 and Cyril G. Gay3

Abstract

Orbiviruses are members of the Reoviridae family and include bluetongue virus (BTV) and epizootic hem- orrhagic disease virus (EHDV). These viruses are the cause of significant regional disease outbreaks among livestock and wildlife in the United States, some of which have been characterized by significant morbidity and mortality. Competent vectors are clearly present in most regions of the globe; therefore, all segments of production livestock are at risk for serious disease outbreaks. Animals with subclinical infections also serve as reservoirs of infection and often result in significant trade restrictions. The economic and explicit impacts of BTV and EHDV infections are difficult to measure, but infections are a cause of economic loss for producers and loss of natural resources (wildlife). In response to United States Animal Health Association (USAHA) Resolution 16, the US Department of Agriculture (USDA), in collaboration with the Department of the Interior (DOI), organized a gap analysis workshop composed of international experts on Orbiviruses. The workshop participants met at the Arthropod-Borne Animal Diseases Research Unit in Manhattan, KS, May 14–16, 2013, to assess the available scientific information and status of currently available countermeasures to effectively control and mitigate the impact of an outbreak of an emerging Orbivirus with epizootic potential, with special emphasis given to BTV and EHDV. In assessing the threats, workshop participants determined that available countermeasures are somewhat effective, but several weaknesses were identified that affect their ability to prevent and control disease outbreaks effectively.

KeyWords: Bluetongue virus—Epizootic hemorrhagic disease—Vector—Vaccine—Diagnostic test—Epidemiology.

Introduction plants, crustaceans, fish, insects, reptiles, and mammals, in- cluding humans (King et al. 2012). luetongue (BT) and epizootic hemorrhagic disease Some 22 distinct virus species of serogroups are within the B(EHD) are noncontagious, insect-transmitted diseases of genus Orbivirus, including BT virus (BTV) and EHD virus domestic and wild ruminants caused by related but distinct (EHDV). To date, 26 distinct serotypes of BTV have been viruses. Both BT and EHD viruses cause hemorrhagic fevers described that all share common group antigens but are dis- in susceptible ruminants; however, BT is a disease of domestic tinguished on the basis of serotype-specific virus neutrali- livestock and certain species of wild ungulates, whereas EHD zation assays (VNTs); at least seven serotypes of EHDV have is principally a disease of wild unglulates. BTV is the pro- been identified (Hofmann et al. 2008, Maan et al. 2011a, b, totype member of the genus Orbivirus, family Reoviridae Savini et al. 2011). Variation among field strains of BTV, (Mertens et al. 2004). All members of the Reoviridae share even those of the same serotype, is considerable, which re- distinctive common properties, including segmented genomes flects differences in the nucleotide sequence of each of the 10 of double-stranded RNA (dsRNA) and characteristic none- distinct dsRNA segments of the BTV genome (Bonneau et al. nveloped, icosahedral virion morphology and structure. There 1999, Pritchard et al. 2004a, b, Balasuriya et al. 2008, Maan are currently two subfamilies and some 16 distinct genera et al. 2010, Maan et al. 2012, Shirafuji et al. 2012, Shaw et al. within the family Reoviridae, which includes pathogens of 2013, Legisa et al. 2013). Genetic heterogeneity of field

1US Department of Agriculture, Agricultural Research Service, Arthropod-Borne Animal Diseases Research Unit, Manhattan, Kansas. 2Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California. 3US Department of Agriculture, Agricultural Research Service, National Program 103–Animal Health, Beltsville, Maryland.

335 336 MCVEY ET AL. strains of BTV occurs as a consequence of both genetic drift treme southeastern United States, was recently isolated from and genetic shift, the latter as a result of reassortment of viral cattle in California (Mecham and Johnson 2005, Maclachlan genes during mixed infections of either the vertebrate or in- et al. 2013, Gaudreault et al. 2014). In a similar manner, vertebrate hosts of the virus (Bonneau and MacLachlan 2004, EHDV-1 and -2 also have been present in the United States Coetzee et al. 2012a, b, Shaw et al. 2013). Variation in the since the 1950s, but new serotypes (EHDV-6) have been in- sequence of individual genes occurs through a complex troduced as recently as 2006 (Allison et al. 2010). The con- process of genetic drift and founder effect during alternating tinual risk of virus introductions into areas with naı¨ve livestock passage of BTV in its ruminant and insect hosts (Bonneau and wildlife populations, as well as the expanding distribution et al. 2001). These phenomena are also complicated by sal- of the Culicoides vector into northern latitudes due to warming ivary gland and midgut barriers to infection and transmission climates, makes BT and EHD a constant threat to livestock by vector midges. Although less well characterized, similar producers. variation also likely occurs among field strains of EHDV Both BTV and EHDV are considered animal pathogens (Cheney et al. 1995, Cheney et al. 1996, Savini et al. 2011). worldwide, and new viral strains are continuously emerging BT is principally a disease of certain breeds of sheep, al- that may cause severe disease outbreaks; however, not every though disease sporadically occurs in cattle and some species susceptible animal species will show disease following in- of wild non-African ruminants (Verwoerd and Erasmus 2004, fection with all strains of these viruses. The differences in Maclachlan et al. 2009, Verwoerd 2012). Epizootic hemor- clinical outcomes depend on the susceptibility of animal rhagic disease is principally a disease of deer, especially hosts and on the pathogenic potential of strains of the viruses. white-tailed deer, although disease sporadically occurs in Hence, the World Organization for Animal Health (Office other wildlife ruminant species and cattle (Hoff and Trainer International des Epizooties, OIE) pragmatically defines 1978, Odiawa et al. 1985, Savini et al. 2011). Orbivirus in- cases of BTV and EHDV as infection with these viruses, with fection of ruminants occurs throughout much of the tem- or without clinical signs. The basis of differences in species perate and tropical regions of the world, coincident with the susceptibility is not known and virulence determinants of distribution of specific species of hematophagous Culicoides Orbiviruses are not described. Comprehensive disease con- (Diptera: Ceratopogonidae), biting midges that are the bio- trol strategies will require investing in basic research to de- logical vectors of the viruses (Gibbs and Greiner 1994, Ta- termine these unknown aspects of the virus–host interaction. bachnick 2004, Tabachnick 2010, Maclachlan 2011). BT The viruses that cause BT and EHD are also of concern to typically occurs when susceptible sheep are introduced into livestock producers in North America because of: (1) The areas where virulent strains of BTV circulate, or when viru- repeated and consistent emergence of new serotypes of lent strains of BTV extend their range into previously un- unknown virulence, (2) increased reports of clinical disease

exposed populations of ruminants. in cattle, and (3) increased spread and adaptation to new The global distribution of BTV generally has been be- geographical areas. Accordingly, the United States Animal tween latitudes of approximately 40–50Nand35S. BTV Health Association (USAHA) passed Resolution 16 in Oc- was spread transiently into southern portions of Mediter- tober of 2012 requesting the United States Department of ranean Europe during the 20th century, but since 1999, Agriculture (USDA) and the United States Department of multiple serotypes have invaded and spread throughout Interior (DOI) to organize a diverse panel of experts, extensive portions of the continent, penetrating farther including industry stakeholders, university and federal re- north than previously documented (Mellor and Leake searchers, and federal and state regulatory agency represen- 2000, Gomez-Tejedor 2004, Mellor et al. 2008, Rodriguez- tatives, to determine research needs and identify and Sanchez et al. 2008). During the recent European BTV-8 prioritize intervention strategies. epizootic, for example, the virus spread throughout Western In response to USAHA Resolution 16, USDA, in collab- Europe (Purse et al. 2005, Toussaint et al. 2006, Purse et al. oration with DOI, organized a gap analysis workshop com- 2008, Wilson and Mellor 2008, Guis et al. 2012). posed of international experts on Orbiviruses. The workshop The global epidemiology of EHDV infection is less pre- participants met at the Arthropod-Borne Animal Diseases cisely defined than that of BTV, but the global range of EHDV Research Unit in Manhattan, Kansas, May 14–16, 2013, to is predicted to occur between latitudes 35Sand49N, coin- assess the available scientific information and status of cur- cident with the distribution of competent biting midge vectors rently available countermeasures to effectively control and (Savini et al. 2011). Unlike BTV infection, EHDV has not mitigate the impact of an outbreak of an emerging Orbivirus been described to date in Europe, although the virus occurs with epizootic. Special emphasis was given to BTV and throughout extensive portions of Asia, Southeast Asia, Aus- EHDV. tralia, Africa, and the Americas (Savini et al. 2011). BT has In assessing the threats, workshop participants determined been recognized in the United States since at least the late that available countermeasures are somewhat effective, but 1940s; BTV serotype 10 (BTV-10) was first isolated and several weaknesses were identified that affect the ability of characterized in California during the early 1950s (McKercher countermeasures to effectively prevent and control disease et al. 1953), and BTV-11, -13, and -17 were identified later outbreaks. In the long term, the workshop participants de- (Barber 1979). BTV-2 was first reported in Florida in 1982, termined that implementing the critical research studies is and since 1998, some 10 additional serotypes of BTV have fundamental to addressing the extensive gaps in our scientific been isolated in the southeastern United States (Gibbs et al. knowledge base. Importantly, this research is paramount to 1983, Collisson et al. 1985, Maclachlan 2011). BTV-1 was advancing the development of effective countermeasures. detected in the southeastern United States in 2004 from a The complete report from the gap analysis workshop can be white-tailed deer isolate (Johnson et al. 2006). Additionally, accessed at the following internet website: http://go.usa.gov/ BTV-2, which was long considered to be confined to the ex- BJ5F. ORBIVIRUSES: A NORTH AMERICAN PERSPECTIVE 337

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