The Preparation and Primary Structure of S-Peptides from Different Pancreatic Ribonucleases
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Cervid TB DPP Testing August 2017
Cervid TB DPP Testing August 2017 Elisabeth Patton, DVM, PhD, Diplomate ACVIM Veterinary Program Manager Wisconsin Department of Agriculture, Trade and Consumer Protection Division of Animal Health Why Use the Serologic Test? Employ newer, accurate diagnostic test technology Minimizes capture and handling events for animal safety Expected to promote additional cervid TB testing • Requested by USAHA and cervid industry Comparable sensitivity and specificity to skin tests 2 Historical Timeline 3 Stat-Pak licensed for elk and red deer, 2009 White-tailed and fallow deer, 2010-11 2010 - USAHA resolution - USDA evaluate Stat-Pak as official TB test 2011 – Project to evaluate TB serologic tests in cervids (Cervid Serology Project); USAHA resolution to approve Oct 2012 – USDA licenses the Dual-Path Platform (DPP) secondary test for elk, red deer, white-tailed deer, and fallow deer Improved specificity compared to Stat-Pak Oct 2012 – USDA approves the Stat-Pak (primary) and DPP (secondary) as official bovine TB tests in elk, red deer, white- tailed deer, fallow deer and reindeer Recent Actions Stat-Pak is no longer in production 9 CFR 77.20 has been amended to approve the DPP as official TB program test. An interim rule was published on 9 January 2013 USDA APHIS created a Guidance Document (6701.2) to provide instructions for using the tests https://www.aphis.usda.gov/animal_health/animal_ diseases/tuberculosis/downloads/vs_guidance_670 1.2_dpp_testing.pdf 4 Cervid Serology Project Objective 5 Evaluate TB detection tests for official bovine tuberculosis (TB) program use in captive and free- ranging cervids North American elk (Cervus canadensis) White-tailed deer (Odocoileus virginianus) Reindeer (Rangifer tarandus) Primary/screening test AND Secondary Test: Dual Path Platform (DPP) Rapid immunochromatographic lateral-flow serology test Detect antibodies to M. -
Phylogenetic Analysis of Eight Sudanese Camel Contagious Ecthyma Viruses Based on B2L Gene Sequence Abdelmalik I
Khalafalla et al. Virology Journal (2015) 12:124 DOI 10.1186/s12985-015-0348-7 RESEARCH Open Access Phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on B2L gene sequence Abdelmalik I. Khalafalla1,2* , Ibrahim M. El-Sabagh3,4, Khalid A. Al-Busada5, Abdullah I. Al-Mubarak6 and Yahia H. Ali7 Abstract Background: Camel contagious ecthyma (CCE) is an important viral disease of camelids caused by a poxvirus of the genus parapoxvirus (PPV) of the family Poxviridae. The disease has been reported in west and east of the Sudan causing economical losses. However, the PPVs that cause the disease in camels of the Sudan have not yet subjected to genetic characterization. At present, the PPV that cause CCE cannot be properly classified because only few isolates that have been genetically analyzed. Methods and results: PCR was used to amplify the B2L gene of the PPV directly from clinical specimens collected from dromedary camels affected with contagious ecthyma in the Sudan between 1993 and 2013. PCR products were sequenced and subjected to genetic analysis. The results provided evidence for close relationships and genetic variation of the camel PPV (CPPV) represented by the circulation of both Pseudocowpox virus (PCPV) and Orf virus (ORFV) strains among dromedary camels in the Sudan. Based on the B2L gene sequence the available CPPV isolates can be divided into two genetic clades or lineages; the Asian lineage represented by isolates from Saudi Arabia, Bahrain and India and the African lineage comprising isolates from the Sudan. Conclusion: The camel parapoxvirus is genetically diverse involving predominantly viruses close to PCPV in addition to ORFVs, and can be divided into two genetically distant lineages. -
Characterization of Caseins from Mongolian Yak, Khainak, and Bactrian Camel B Ochirkhuyag, Jm Chobert, M Dalgalarrondo, Y Choiset, T Haertlé
Characterization of caseins from Mongolian yak, khainak, and bactrian camel B Ochirkhuyag, Jm Chobert, M Dalgalarrondo, Y Choiset, T Haertlé To cite this version: B Ochirkhuyag, Jm Chobert, M Dalgalarrondo, Y Choiset, T Haertlé. Characterization of caseins from Mongolian yak, khainak, and bactrian camel. Le Lait, INRA Editions, 1997, 77 (5), pp.601-613. hal-00929550 HAL Id: hal-00929550 https://hal.archives-ouvertes.fr/hal-00929550 Submitted on 1 Jan 1997 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Lait (1997) 77, 601-613 601 © Eisevier/Inra Original article Characterization of caseins from Mongolian yak, khainak, and bactrian cam el B Ochirkhuyag 2, lM Chobert 1*, M Dalgalarrondo 1, Y Choiset 1, T Haertlé ' 1 Laboratoire d'étude des interactions des molécules alimentaires, Inra, rue de la Géraudière, BP 71627, 44316 Nantes cedex 03, France; 2 Institute of Chemistry, Academy of Sciences, Vlan Bator, Mongolia (Received 25 November 1996; accepted 5 May 1997) Summary - The composition of acid-precipitated caseins from ruminant Mongolian domestic ani- maIs was analyzed and a comparative study between camel (Camelus bactrianus) and dromedary (Camelus dromedarius) was realized. Acid-precipitated whole caseins were analyzed for ami no acid composition, separated by anion exchange chromatography and identified by alkaline urea-PAGE. -
Muskox Management Report Alaska Dept of Fish and Game Wildlife
Muskox Management Report of survey-inventory activities 1 July 1998–30 June 2000 Mary V. Hicks, Editor Alaska Department of Fish and Game Division of Wildlife Conservation December 2001 ADF&G Please note that population and harvest data in this report are estimates and may be refined at a later date. If this report is used in its entirety, please reference as: Alaska Department of Fish and Game. 2001. Muskox management report of survey-inventory activities 1 July 1998–30 June 2000. M.V. Hicks, editor. Juneau, Alaska. If used in part, the reference would include the author’s name, unit number, and page numbers. Authors’ names can be found at the end of each unit section. Funded in part through Federal Aid in Wildlife Restoration, Proj. 16, Grants W-27-2 and W-27-3. LOCATION 2 GAME MANAGEMENT UNIT: 18 (41,159 mi ) GEOGRAPHIC DESCRIPTION: Yukon–Kuskokwim Delta BACKGROUND NUNIVAK ISLAND Muskoxen were once widely distributed in northern and western Alaska but were extirpated by the middle or late 1800s. In 1929, with the support of the Alaska Territorial Legislature, the US Congress initiated a program to reintroduce muskoxen in Alaska. Thirty-one muskoxen were introduced from Greenland to Nunivak Island in Unit 18 during 1935–1936, as a first step toward reintroducing this species to Alaska. The Nunivak Island population grew slowly until approximately 1958 and then began a period of rapid growth. The first hunting season was opened in 1975, and the population has since fluctuated between 400 and 750 animals, exhibiting considerable reproductive potential, even under heavy harvest regimes. -
What Do Caribou and Wood Bison Have in Common? by Nate Olson
Refuge Notebook • Vol. 17, No. 4 • January 23, 2015 What do caribou and wood bison have in common? by Nate Olson Residents from the village of Shageluk on the Innoko River assist ADFG and USFWS staff in constructing a holding pen as part of the wood bison reintroduction effort. 100 wood bison are scheduled to be released on the lower Innoko River in March 2015. Photo credit: Tom Seaton, ADFG. Last weekend on a drive back from Anchorage, Wood bison are native to Alaska and were plentiful whizzing by the Alaska Wildlife Conservation Center over a large portion of the state until their extirpation at 57 miles per hour my daughter pointed out a strange in the early 1900s. The reasons for their disappearance looking moose on the side of the Seward Highway. are not clearly known but probably related to overhar- “That isn’t a moose,” I explained, “that is a woodbi- vest and habitat loss. son.” Wildlife transplants are nothing new in Alaska. And why are wood bison living in pens next to The reasons for transplanting animals generally fall the Seward Highway? They are part of a reintroduc- into two categories. The first is to provide human re- tion effort by the Alaska Department of Fish and Game lated benefits such as recreational hunting, economic (ADFG) and the Alaska Wildlife Conservation Center. gain, or an additional food supply. The second is re- The fate of these wood bison has been in the news lated to species recovery in their historic range. lately as the U.S. Fish and Wildlife Service (USFWS) Kodiak Island is a dramatic example of the first published a final rule in May 2014 that gives the green category. -
The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (Rangifer Tarandus L.) from the Russian Arctic Zone: Regional and Seasonal Effects
animals Article The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (Rangifer tarandus L.) from the Russian Arctic Zone: Regional and Seasonal Effects Larisa A. Ilina 1,*, Valentina A. Filippova 1 , Evgeni A. Brazhnik 1 , Andrey V. Dubrovin 1, Elena A. Yildirim 1 , Timur P. Dunyashev 1, Georgiy Y. Laptev 1, Natalia I. Novikova 1, Dmitriy V. Sobolev 1, Aleksandr A. Yuzhakov 2 and Kasim A. Laishev 2 1 BIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, Russia; fi[email protected] (V.A.F.); [email protected] (E.A.B.); [email protected] (A.V.D.); [email protected] (E.A.Y.); [email protected] (T.P.D.); [email protected] (G.Y.L.); [email protected] (N.I.N.); [email protected] (D.V.S.) 2 Department of Animal Husbandry and Environmental Management of the Arctic, Federal Research Center of Russian Academy Sciences, 7, Sh. Podbel’skogo, Pushkin, 196608 St. Petersburg, Russia; [email protected] (A.A.Y.); [email protected] (K.A.L.) * Correspondence: [email protected] Simple Summary: The reindeer (Rangifer tarandus) is a unique ruminant that lives in arctic areas characterized by severe living conditions. Low temperatures and a scarce diet containing a high Citation: Ilina, L.A.; Filippova, V.A.; proportion of hard-to-digest components have contributed to the development of several adaptations Brazhnik, E.A.; Dubrovin, A.V.; that allow reindeer to have a successful existence in the Far North region. These adaptations include Yildirim, E.A.; Dunyashev, T.P.; Laptev, G.Y.; Novikova, N.I.; Sobolev, the microbiome of the rumen—a digestive organ in ruminants that is responsible for crude fiber D.V.; Yuzhakov, A.A.; et al. -
Camel Prion Disease: a Possible Emerging Disease in Dromedary Camel Populations?
Camel prion disease: a possible emerging disease in dromedary camel populations? ©B. Babelhadj/University Kasdi Merbah, Algeria The identification of a new prion disease in dromedary camels in Algeria and Tunisia, called camel prion disease (CPD), extends the spectrum of animal species naturally susceptible to prion diseases and opens up new research areas for investigation. Camel prion disease was identified in 2018 in adult camels showing clinical signs at the ante mortem inspection at slaughterhouses in the region of Ouargla (Algeria), and in 2019 in the region of Tataouine (Tunisia). It adds to the group of existing animal prion diseases, including scrapie in sheep and goats, chronic wasting disease (CWD) in cervids and BSE (mainly in bovines). The detection of a new prion disease in the dromedary population requires attention and investigation needs to be carried out to assess the risks of this disease to animal and public health. As of today, very limited epidemiological information is available to assess the prevalence, geographical distribution and dynamic of the transmission of the disease. Based on the clinical signs suggesting prion disease, CPD seems to have occurred in 3.1% of the dromedaries brought to the abattoir in Ouargla. Pathognomonic neurodegeneration and disease- specific prion protein (PrPSc) were detected in brain tissue from three symptomatic animals (source: CDC article wwwnc.cdc.gov/eid/article/24/6/17-2007_article). In May 2019, the OIE received a report from Tunisia on a single case of a 12-year-old slaughtered dromedary camel showing neurological signs confirmed as CPD by the Istituto Superiore di Sanità (ISS) based in Italy. -
Foraging Ecologies of Giraffe (Giraffa Camelopardalis Reticulata)
Foraging ecologies of giraffe (Giraffa camelopardalis reticulata) and camels (Camelus dromedarius) in northern Kenya: effects of habitat structure and possibilities for competition? David A. O’Connor1,2,3*, Bilal Butt2 and Johannes B. Foufopoulos2 1San Diego Zoo Institute for Conservation Research, 15600 San Pasqual Valley Road, Escondido, CA, 92027, U.S.A., 2School of Natural Resources and Environment, University of Michigan, 440 Church St. Ann Arbor, Michigan, 48109-1041, U.S.A. and 3National Geographic Society 1145 17th St., NW, Washington, DC, 20036, U.S.A. Abstract au Kenya, ou ces especes sont recemment devenues The foraging ecologies of reticulated giraffe (Giraffa camel- sympatriques. La popularite croissante des dromadaires opardalis reticulata) and domestic camels (Camelus drome- dans la region a suscite des inquietudes au sujet des darius) were examined in the Laikipia District of Kenya, impacts sur l’environnement et d’une eventuelle competi- where these species have recently become sympatric. tion pour les ressources avec les girafes sauvages. Nous Camels increased popularity in the region has lead to avons recolte des donnees sur l’alimentation des deux concerns about their environmental impacts and possible especes au moyen de scan de groupe de deux minutes, qui competition with wild giraffe for resources. We gathered enregistraient la hauteur a laquelle les animaux mangea- foraging data on both species using 2-min group scans ient et les plantes preferees. Des transects ont permis de that recorded feeding heights and plant food preferences. recolter des echantillons de vegetation dans les zones ou les Transects sampled the vegetation in areas where foraging observations alimentaires ont ete faites. -
Camelus Dromedarius) Need Shaded Areas? a Case Study of the Camel Market in Doha
animals Article Do Camels (Camelus dromedarius) Need Shaded Areas? A Case Study of the Camel Market in Doha Martina Zappaterra, Laura Menchetti , Leonardo Nanni Costa and Barbara Padalino * Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin 46, I-40127 Bologna, Italy; [email protected] (M.Z.); [email protected] (L.M.); [email protected] (L.N.C.) * Correspondence: [email protected] Simple Summary: Scientific knowledge concerning dromedary camel behavior and welfare is still limited. To date, providing pens with adequate shaded areas is not regulated in camel husbandry. The objectives of this study were to document whether dromedary camels have a preference for shade and describe how their behavior would change depending on the presence of shade in pens with different animal densities. Analyzing the behavior of camels kept at a permanent market in Doha, we found they had a preference for shade, and adequate shaded areas seemed to exert a positive effect on their behavioral repertoire. Camels in shade expressed more natural behaviors such as lying in sternal recumbency and ruminating, while those in the sun showed more walking and standing. Limited space allowance, instead, seemed to affect camel welfare, increasing the expression of stereotypic behavior (i.e., pacing). Overall, the results of this pilot study suggest that provision of adequate shaded areas could safeguard camel wellbeing under extremely hot conditions. Citation: Zappaterra, M.; Menchetti, Abstract: This study aimed at documenting whether dromedary camels have a preference for shade L.; Nanni Costa, L.; Padalino, B. Do and how their behavior would change depending on the presence of shade and variable space Camels (Camelus dromedarius) Need allowance. -
Cervid Mixed-Species Table That Was Included in the 2014 Cervid RC
Appendix III. Cervid Mixed Species Attempts (Successful) Species Birds Ungulates Small Mammals Alces alces Trumpeter Swans Moose Axis axis Saurus Crane, Stanley Crane, Turkey, Sandhill Crane Sambar, Nilgai, Mouflon, Indian Rhino, Przewalski Horse, Sable, Gemsbok, Addax, Fallow Deer, Waterbuck, Persian Spotted Deer Goitered Gazelle, Reeves Muntjac, Blackbuck, Whitetailed deer Axis calamianensis Pronghorn, Bighorned Sheep Calamian Deer Axis kuhili Kuhl’s or Bawean Deer Axis porcinus Saurus Crane Sika, Sambar, Pere David's Deer, Wisent, Waterbuffalo, Muntjac Hog Deer Capreolus capreolus Western Roe Deer Cervus albirostris Urial, Markhor, Fallow Deer, MacNeil's Deer, Barbary Deer, Bactrian Wapiti, Wisent, Banteng, Sambar, Pere White-lipped Deer David's Deer, Sika Cervus alfredi Philipine Spotted Deer Cervus duvauceli Saurus Crane Mouflon, Goitered Gazelle, Axis Deer, Indian Rhino, Indian Muntjac, Sika, Nilgai, Sambar Barasingha Cervus elaphus Turkey, Roadrunner Sand Gazelle, Fallow Deer, White-lipped Deer, Axis Deer, Sika, Scimitar-horned Oryx, Addra Gazelle, Ankole, Red Deer or Elk Dromedary Camel, Bison, Pronghorn, Giraffe, Grant's Zebra, Wildebeest, Addax, Blesbok, Bontebok Cervus eldii Urial, Markhor, Sambar, Sika, Wisent, Waterbuffalo Burmese Brow-antlered Deer Cervus nippon Saurus Crane, Pheasant Mouflon, Urial, Markhor, Hog Deer, Sambar, Barasingha, Nilgai, Wisent, Pere David's Deer Sika 52 Cervus unicolor Mouflon, Urial, Markhor, Barasingha, Nilgai, Rusa, Sika, Indian Rhino Sambar Dama dama Rhea Llama, Tapirs European Fallow Deer -
Amplification of Potential Thermogenetic Mechanisms In
www.nature.com/scientificreports OPEN Amplifcation of potential thermogenetic mechanisms in cetacean brains compared to artiodactyl brains Paul R. Manger1*, Nina Patzke1,10, Muhammad A. Spocter1,2, Adhil Bhagwandin1,11, Karl Æ. Karlsson3, Mads F. Bertelsen4, Abdulaziz N. Alagaili5, Nigel C. Bennett5,6, Osama B. Mohammed5, Suzana Herculano‑Houzel7, Patrick R. Hof8 & Kjell Fuxe9 To elucidate factors underlying the evolution of large brains in cetaceans, we examined 16 brains from 14 cetartiodactyl species, with immunohistochemical techniques, for evidence of non‑shivering thermogenesis. We show that, in comparison to the 11 artiodactyl brains studied (from 11 species), the 5 cetacean brains (from 3 species), exhibit an expanded expression of uncoupling protein 1 (UCP1, UCPs being mitochondrial inner membrane proteins that dissipate the proton gradient to generate heat) in cortical neurons, immunolocalization of UCP4 within a substantial proportion of glia throughout the brain, and an increased density of noradrenergic axonal boutons (noradrenaline functioning to control concentrations of and activate UCPs). Thus, cetacean brains studied possess multiple characteristics indicative of intensifed thermogenetic functionality that can be related to their current and historical obligatory aquatic niche. These fndings necessitate reassessment of our concepts regarding the reasons for large brain evolution and associated functional capacities in cetaceans. Cetaceans (whales, dolphins and porpoises) in general have large relative and absolute brain -
Camel Anatomy; More Than Just a Hump
The Review: A Journal of Undergraduate Student Research Volume 20 Article 5 2019 Camel Anatomy; More Than Just a Hump Michael Chase St. John Fisher College, [email protected] Follow this and additional works at: https://fisherpub.sjfc.edu/ur Part of the Anatomy Commons How has open access to Fisher Digital Publications benefited ou?y Recommended Citation Chase, Michael. "Camel Anatomy; More Than Just a Hump." The Review: A Journal of Undergraduate Student Research 20 (2019): -. Web. [date of access]. <https://fisherpub.sjfc.edu/ur/vol20/iss1/5>. This document is posted at https://fisherpub.sjfc.edu/ur/vol20/iss1/5 and is brought to you for free and open access by Fisher Digital Publications at St. John Fisher College. For more information, please contact [email protected]. Camel Anatomy; More Than Just a Hump Abstract The one-humped camel (Camelus dromedarius) is capable of living in extreme, arid environments due to its numerous anatomical adaptations. Its modified eaturf es of the muscular system, integument, skeletal system, and several internal organs allow this animal to survive in such harsh environmental conditions. Many of these adaptations allow for conservation of energy and water as well as improvement of locomotion to acquire scarce resources. In this paper we will look more closely at some of these adaptations and determine their function in promoting the survival and reproduction of the one-humped camel in desert environments. Keywords camel, anatomy, hump, camelus, dromedarius, desert, anatomy This article is available in The Review: A Journal of Undergraduate Student Research: https://fisherpub.sjfc.edu/ur/ vol20/iss1/5 Chase: Camel Anatomy; More Than Just a Hump The Review, Volume 20 (2019) Camel Anatomy: More Than Just a Hump Michael Chase ABSTRACT The one-humped camel (Camelus dromedarius) is capable of living in extreme, arid environments due to its numerous anatomical adaptations.