MINUTES of the MEETING of the S-9 TECHNICAL COMMITTEE
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CHLOROPLAST Matk GENE PHYLOGENY of SOME IMPORTANT SPECIES of PLANTS
AKDENİZ ÜNİVERSİTESİ ZİRAAT FAKÜLTESİ DERGİSİ, 2005, 18(2), 157-162 CHLOROPLAST matK GENE PHYLOGENY OF SOME IMPORTANT SPECIES OF PLANTS Ayşe Gül İNCE1 Mehmet KARACA2 A. Naci ONUS1 Mehmet BİLGEN2 1Akdeniz University Faculty of Agriculture Department of Horticulture, 07059 Antalya, Turkey 2Akdeniz University Faculty of Agriculture Department of Field Crops, 07059 Antalya, Turkey Correspondence addressed E-mail: [email protected] Abstract In this study using the chloroplast matK DNA sequence, a chloroplast-encoded locus that has been shown to be much more variable than many other genes, from one hundred and forty two plant species belong to the families of 26 plants we conducted a study to contribute to the understanding of major evolutionary relationships among the studied plant orders, families genus and species (clades) and discussed the utilization of matK for molecular phylogeny. Determined genetic relationship between the species or genera is very valuable for genetic improvement studies. The chloroplast matK gene sequences ranging from 730 to 1545 nucleotides were downloaded from the GenBank database. These DNA sequences were aligned using Clustal W program. We employed the maximum parsimony method for phylogenetic reconstruction using PAUP* program. Trees resulting from the parsimony analyses were similar to those generated earlier using single or multiple gene analyses, but our analyses resulted in strict consensus tree providing much better resolution of relationships among major clades. We found that gymnosperms (Pinus thunbergii, Pinus attenuata and Ginko biloba) were different from the monocotyledons and dicotyledons. We showed that Cynodon dactylon, Panicum capilare, Zea mays and Saccharum officiarum (all are in the C4 metabolism) were improved from a common ancestors while the other cereals Triticum Avena, Hordeum, Oryza and Phalaris were evolved from another or similar ancestors. -
Production of Disomic Wheat-Barley Chromosome Addition Lines Using Hordeum Bulbosum Crosses
Genet. Res., Gamb. (1981), 37, pp. 215-219 215 Printed in Great Britain SHORT PAPER Production of disomic wheat-barley chromosome addition lines using Hordeum bulbosum crosses BY A. K. M. R. ISLAM AND K. W. SHEPHERD Department of Agronomy, Waite Agricultural Research Institute, The University of Adelaide, Glen Osmond, South Australia, 5064 (Received 1 September 1980) SUMMARY The possibility of using Hordeum bulbosum Crosses to facilitate production of disomic wheat-barley addition lines from monosomic additions was investigated. Aneuhaploids with 22 chromosomes were obtained in the expected gametic frequencies after crossing monosomic, disomio and monotelo-disomic addition lines, involving four different barley chromosomes, as the female parent with tetraploid H. bulbosum. Thus the added barley chromosomes were not eliminated when preferential elimination of the bulbosum chromo- somes took place in the hybrid embryos. Disomic addition lines were obtained after treating the aneuhaploids with colchicine. This method could have wider application in the production of other wheat-alien chromosome disomic addition lines, especially where the transmission frequency of the alien chromosome through the pollen is very low, but its use will depend on the wheat parent being crossable with H. bulbosum and the alien chromosome being retained during the elimination of bulbosum chromosomes. 1. INTRODUCTION After O'Mara (1940) described a systematic procedure for adding individual pairs of rye chromosomes to wheat, many other workers have used a similar approach to add chromosomes of several other alien species to wheat. These addition lines have had many uses including assigning genes in the alien species to particular chromosomes, determining the genetic similarity (homoeology) between alien chromosomes and particular wheat chromosomes, and also for transferring desirable characters such as disease resistance from the alien species to wheat. -
Texas Big Tree Registry a List of the Largest Trees in Texas Sponsored by Texas a & M Forest Service
Texas Big Tree Registry A list of the largest trees in Texas Sponsored by Texas A & M Forest Service Native and Naturalized Species of Texas: 320 ( D indicates species naturalized to Texas) Common Name (also known as) Latin Name Remarks Cir. Threshold acacia, Berlandier (guajillo) Senegalia berlandieri Considered a shrub by B. Simpson 18'' or 1.5 ' acacia, blackbrush Vachellia rigidula Considered a shrub by Simpson 12'' or 1.0 ' acacia, Gregg (catclaw acacia, Gregg catclaw) Senegalia greggii var. greggii Was named A. greggii 55'' or 4.6 ' acacia, Roemer (roundflower catclaw) Senegalia roemeriana 18'' or 1.5 ' acacia, sweet (huisache) Vachellia farnesiana 100'' or 8.3 ' acacia, twisted (huisachillo) Vachellia bravoensis Was named 'A. tortuosa' 9'' or 0.8 ' acacia, Wright (Wright catclaw) Senegalia greggii var. wrightii Was named 'A. wrightii' 70'' or 5.8 ' D ailanthus (tree-of-heaven) Ailanthus altissima 120'' or 10.0 ' alder, hazel Alnus serrulata 18'' or 1.5 ' allthorn (crown-of-thorns) Koeberlinia spinosa Considered a shrub by Simpson 18'' or 1.5 ' anacahuita (anacahuite, Mexican olive) Cordia boissieri 60'' or 5.0 ' anacua (anaqua, knockaway) Ehretia anacua 120'' or 10.0 ' ash, Carolina Fraxinus caroliniana 90'' or 7.5 ' ash, Chihuahuan Fraxinus papillosa 12'' or 1.0 ' ash, fragrant Fraxinus cuspidata 18'' or 1.5 ' ash, green Fraxinus pennsylvanica 120'' or 10.0 ' ash, Gregg (littleleaf ash) Fraxinus greggii 12'' or 1.0 ' ash, Mexican (Berlandier ash) Fraxinus berlandieriana Was named 'F. berlandierana' 120'' or 10.0 ' ash, Texas Fraxinus texensis 60'' or 5.0 ' ash, velvet (Arizona ash) Fraxinus velutina 120'' or 10.0 ' ash, white Fraxinus americana 100'' or 8.3 ' aspen, quaking Populus tremuloides 25'' or 2.1 ' baccharis, eastern (groundseltree) Baccharis halimifolia Considered a shrub by Simpson 12'' or 1.0 ' baldcypress (bald cypress) Taxodium distichum Was named 'T. -
Oaks of the Wild West Inventory Page 1 Nursery Stock Feb, 2016
Oaks of the Wild West Inventory Nursery Stock Legend: AZ = Arizona Nursery TX = Texas Nursery Feb, 2016 *Some species are also available in tube sizes Pine Trees Scientific Name 1G 3/5G 10G 15 G Aleppo Pine Pinus halapensis AZ Afghan Pine Pinus elderica AZ Apache Pine Pinus engelmannii AZ Chinese Pine Pinus tabulaeformis AZ Chihuahua Pine Pinus leiophylla Cluster Pine Pinus pinaster AZ Elderica Pine Pinus elderica AZ AZ Italian Stone Pine Pinus pinea AZ Japanese Black Pine Pinus thunbergii Long Leaf Pine Pinus palustris Mexican Pinyon Pine Pinus cembroides AZ Colorado Pinyon Pine Pinus Edulis AZ Ponderosa Pine Pinus ponderosa AZ Scotch Pine Pinus sylvestre AZ Single Leaf Pine Pinus monophylla AZ Texas Pine Pinus remota AZ, TX Common Trees Scientific Name 1G 3/5G 10G 15 G Arizona Sycamore Platanus wrightii ** Ash, Arizona Fraxinus velutina AZ AZ Black Walnut, Arizona Juglans major AZ AZ Black Walnut, Texas Juglans microcarpa TX Black Walnut juglans nigra AZ, TX Big Tooth Maple Acer grandidentatum AZ Carolina Buckthorn Rhamnus caroliniana TX Chitalpa Chitalpa tashkentensis AZ Crabapple, Blanco Malus ioensis var. texana Cypress, Bald Taxodium distichum AZ Desert Willow Chillopsis linearis AZ AZ Elm, Cedar Ulmus crassifolia TX TX Ginko Ginkgo biloba TX Hackberry, Canyon Celtis reticulata AZ AZ AZ Hackberry, Common Celtis occidentalis TX Maple (Sugar) Acer saccharum AZ AZ Mexican Maple Acer skutchii AZ Mexican Sycamore Platanus mexicana ** Mimosa, fragrant Mimosa borealis Page 1 Oaks of the Wild West Inventory Pistache (Red Push) Pistacia -
Having Gametic Chromosome Number Are Called Haploids
Session-10 Classification of Haploids Haploids: Plants (or the sporophyte) having gametic chromosome number are called haploids. Classification of Haploids: According to their cytological features, haploids have been classified into different types (Fig. 17.1), a brief description of which is given below. I. Euhaploid: The chromosome number of such a haploid is an exact multiple of one of the basic numbers of the group. Euhaploids are of two types: i) Mono-haploid or monoploid: It arises from a diploid species (2x) and possesses the gametic chromosome number of the species; it is designated as “x”. (ii) Polyhaploid: Haploid individuals arising from a polyploid species (4x (autotetraploid), 6x (autohexaploid), etc.,) are called polyhaploids; they may be di-haploids (with 2x), tri- haploids (3x) and so on. Based on their auto- or allo-ploid condition, polyhaploids have been divided into two groups: (a) Auto-polyhaploid : Autopolyploid species give rise to auto-polyhaploids. For example, Autotetraploid (Ax = AAAA)———-> Auto-di-haploid (2n = AA) Auto-hexaploid (6x = AAAAAA)———–> Auto-tri-haploid (3x = AAA) (b) Allopolyhaploid : Such individuals arise from allopolyploid species. For example, Allotetraploid {Ax = AABB)———-> Allodihaploid (2x = AB) Allohexaploid (6x = AABBDD)———–> Allotrihaploid (3x = ABD) II. Aneuhaploid: The chromosome number of such a haploid is not an exact multiple of the basic number of the group. Aneuhaploids have been classified into five types: (i) Disomic haploids (n + 1): Such haploids have one chromosome in disomic condition, i.e., there is one extra chromosome belonging to the genome involved. (ii) Addition haploids (n + few alien): The extra chromosome(s) in the haploid is an alien chromosome. -
Illustrated Flora of East Texas Illustrated Flora of East Texas
ILLUSTRATED FLORA OF EAST TEXAS ILLUSTRATED FLORA OF EAST TEXAS IS PUBLISHED WITH THE SUPPORT OF: MAJOR BENEFACTORS: DAVID GIBSON AND WILL CRENSHAW DISCOVERY FUND U.S. FISH AND WILDLIFE FOUNDATION (NATIONAL PARK SERVICE, USDA FOREST SERVICE) TEXAS PARKS AND WILDLIFE DEPARTMENT SCOTT AND STUART GENTLING BENEFACTORS: NEW DOROTHEA L. LEONHARDT FOUNDATION (ANDREA C. HARKINS) TEMPLE-INLAND FOUNDATION SUMMERLEE FOUNDATION AMON G. CARTER FOUNDATION ROBERT J. O’KENNON PEG & BEN KEITH DORA & GORDON SYLVESTER DAVID & SUE NIVENS NATIVE PLANT SOCIETY OF TEXAS DAVID & MARGARET BAMBERGER GORDON MAY & KAREN WILLIAMSON JACOB & TERESE HERSHEY FOUNDATION INSTITUTIONAL SUPPORT: AUSTIN COLLEGE BOTANICAL RESEARCH INSTITUTE OF TEXAS SID RICHARDSON CAREER DEVELOPMENT FUND OF AUSTIN COLLEGE II OTHER CONTRIBUTORS: ALLDREDGE, LINDA & JACK HOLLEMAN, W.B. PETRUS, ELAINE J. BATTERBAE, SUSAN ROBERTS HOLT, JEAN & DUNCAN PRITCHETT, MARY H. BECK, NELL HUBER, MARY MAUD PRICE, DIANE BECKELMAN, SARA HUDSON, JIM & YONIE PRUESS, WARREN W. BENDER, LYNNE HULTMARK, GORDON & SARAH ROACH, ELIZABETH M. & ALLEN BIBB, NATHAN & BETTIE HUSTON, MELIA ROEBUCK, RICK & VICKI BOSWORTH, TONY JACOBS, BONNIE & LOUIS ROGNLIE, GLORIA & ERIC BOTTONE, LAURA BURKS JAMES, ROI & DEANNA ROUSH, LUCY BROWN, LARRY E. JEFFORDS, RUSSELL M. ROWE, BRIAN BRUSER, III, MR. & MRS. HENRY JOHN, SUE & PHIL ROZELL, JIMMY BURT, HELEN W. JONES, MARY LOU SANDLIN, MIKE CAMPBELL, KATHERINE & CHARLES KAHLE, GAIL SANDLIN, MR. & MRS. WILLIAM CARR, WILLIAM R. KARGES, JOANN SATTERWHITE, BEN CLARY, KAREN KEITH, ELIZABETH & ERIC SCHOENFELD, CARL COCHRAN, JOYCE LANEY, ELEANOR W. SCHULTZE, BETTY DAHLBERG, WALTER G. LAUGHLIN, DR. JAMES E. SCHULZE, PETER & HELEN DALLAS CHAPTER-NPSOT LECHE, BEVERLY SENNHAUSER, KELLY S. DAMEWOOD, LOGAN & ELEANOR LEWIS, PATRICIA SERLING, STEVEN DAMUTH, STEVEN LIGGIO, JOE SHANNON, LEILA HOUSEMAN DAVIS, ELLEN D. -
Coinoculación De Rizobios Y HMA Para El Establecimiento De Stylosanthes
Instituto Nacional de Ciencias Agrícolas Coinoculación de rizobios y hongos micorrízicos arbusculares para el establecimiento de Stylosanthes guianensis en asociación con Brachiaria decumbens Tesis presentada en opción al título académico de Maestro en Ciencias en Nutrición de las Plantas y Biofertilizantes Autor: Ing. Gustavo Crespo Flores Tutor: Dr. C. Pedro José González Cañizares San José de las Lajas, Mayabeque 2016 Dedicatoria A mis padres A mis hijos y A mi esposa AGRADECIMIENTOS A la Revolución Cubana, por posibilitar mis estudios y mi formación profesional. A mi padre y mi madre, por guiarme con la luz de su ejemplo, en el trabajo y conducta en general, por su apoyo constante y la confianza depositada en mí. A mi esposa, por su apoyo diario y a mis tres hijos (Thalía, Danny Y. y Dariel G.), por ser mi principal motivación para seguir adelante. A toda mi familia, desde el más pequeño hasta el más longevo, los que están lejos y los de cerca, y con los que convivo diariamente. A mi tutor, Dr. C. Pedro José González, por su constante apoyo en el desarrollo de este trabajo y mi trayectoria profesional en estos últimos años. A aquellos a los que considero verdaderos maestros de la enseñanza profesional, quienes me han guiado de alguna manera para desarrollar este trabajo, los Dr. C. Rodolfo Plana, Nicolás Medina, Alberto Hernández, entre otros y los Ms. C. Jorge Corbera, Luis R. Fundora y Alfredo Calderón. A los miembros de la Comisión Científica del departamento de Biofertilizantes y Nutrición de las Plantas por sus consejos y sugerencias, especialmente a mi oponente Dr. -
Fragrant Annuals Fragrant Annuals
TheThe AmericanAmerican GARDENERGARDENER® TheThe MagazineMagazine ofof thethe AAmericanmerican HorticulturalHorticultural SocietySociety JanuaryJanuary // FebruaryFebruary 20112011 New Plants for 2011 Unusual Trees with Garden Potential The AHS’s River Farm: A Center of Horticulture Fragrant Annuals Legacies assume many forms hether making estate plans, considering W year-end giving, honoring a loved one or planting a tree, the legacies of tomorrow are created today. Please remember the American Horticultural Society when making your estate and charitable giving plans. Together we can leave a legacy of a greener, healthier, more beautiful America. For more information on including the AHS in your estate planning and charitable giving, or to make a gift to honor or remember a loved one, please contact Courtney Capstack at (703) 768-5700 ext. 127. Making America a Nation of Gardeners, a Land of Gardens contents Volume 90, Number 1 . January / February 2011 FEATURES DEPARTMENTS 5 NOTES FROM RIVER FARM 6 MEMBERS’ FORUM 8 NEWS FROM THE AHS 2011 Seed Exchange catalog online for AHS members, new AHS Travel Study Program destinations, AHS forms partnership with Northeast garden symposium, registration open for 10th annual America in Bloom Contest, 2011 EPCOT International Flower & Garden Festival, Colonial Williamsburg Garden Symposium, TGOA-MGCA garden photography competition opens. 40 GARDEN SOLUTIONS Plant expert Scott Aker offers a holistic approach to solving common problems. 42 HOMEGROWN HARVEST page 28 Easy-to-grow parsley. 44 GARDENER’S NOTEBOOK Enlightened ways to NEW PLANTS FOR 2011 BY JANE BERGER 12 control powdery mildew, Edible, compact, upright, and colorful are the themes of this beating bugs with plant year’s new plant introductions. -
Tree Recommendations
New Mexico State University, Cooperative Extension Service www.aces.nmsu.edu Shrubs Vines Trees Palms Citrus ‘Landscape Trees in the Southwest’ Discovering the beautiful possibilities of what can be grown in our southwestern desert climates Washington D.C. Tree Canopies for Walkability of City Streets, Reduction of the Heat Island Effect Red Oaks Tree Groups to Consider Zelkova/Elm trees Fagaceae/Oak Family Heritage Seedlings and Liners, Salem, Oregon (heritageseedlings.com) ‘Mesa de Maya’ Oak Quercus grisea ‘Grey Oak’ Quercus muhlenbergii ‘Chinquapin Oak’ Quercus rugosa ‘Net Leaf Oak’ Quercus laceyi ‘Lacey Oak’ Quercus oblongifolia ‘Mexican Blue Oak’ Quercus robur x alba 'Crimschmidt‘ ‘CRIMSON SPIRE OAK’ Quercus albocicta ‘Cusi Oak’ Yécora, Sonora, Mexico Quercus germana ‘Mexican Royal Oak’ Ulmaceae/Elm Family Celtis reticulata ‘Net Leaf Hackberry’ Sapindaceae/Maple Family Sapindus drummondii ‘Western Soapberry’ Ungnadia speciosa ‘Mexican Buckeye’ Acer grandidentatum ‘Big Tooth Maple’ ‘Big Tooth Maple’ Acer grandidentatum ‘Mesa Glow’ NMSU, New Tree Introduction through J. Frank Schmidt & Son Company, Boring, OR Acer sempervirens ‘Cretan Maple’ Acer sempervirens ‘Cretan Maple’ Cashew/Anacardiaceae Family Pistacia chinensis Pistacia chinensis ‘Keith Davey’ Pistacia ‘Red Push’ Pistacia mexicana Pistacia lentiscus ‘Mastic Gum Tree’ Pistacia lentiscus Pistacia lentiscus Rhus ovata Rhus ovata Olive/Oleaceae Family Chionanthus retusus ‘Chinese Fringe Tree’ Chionanthus retusus ‘Chinese Fringe Tree’ Foresteria neomexicana Fraxinus greggii ‘Little -
Tobusch Fishhook Cactus Species Status Assessment - Final
Tobusch Fishhook Cactus Species Status Assessment - Final SPECIES STATUS ASSESSMENT REPORT FOR TOBUSCH FISHHOOK CACTUS (SCLEROCACTUS BREVIHAMATUS SSP. TOBUSCHII (W.T. MARSHALL) N.P. TAYLOR) February, 2017 Southwest Region U.S. Fish and Wildlife Service Albuquerque, NM Tobusch Fishhook Cactus Species Status Assessment - Final Prepared by Chris Best, Austin Ecological Services Field Office, Suggested citation: U.S. Fish and Wildlife Service. 2017. Species status assessment of Tobusch Fishhook Cactus (Sclerocactus brevihamatus ssp. tobuschii (W.T. Marshall) N.P. Taylor). U.S. Fish and Wildlife Service Southwest Region, Albuquerque, New Mexico. 65 pp. + 2 appendices. i Tobusch Fishhook Cactus Species Status Assessment - Final EXECUTIVE SUMMARY Tobusch fishhook cactus is a small cactus, with curved “fishhook” spines, that is endemic to the Edwards Plateau of Texas. It was federally listed as endangered on November 7, 1979 (44 FR 64736) as Ancistrocactus tobuschii. At that time, fewer than 200 individuals had been documented from 4 sites. Tobusch fishhook cactus is now confirmed in 8 central Texas counties: Bandera, Edwards, Kerr, Kimble, Kinney, Real, Uvalde, and Val Verde. In recent years, over 4,000 individuals have been documented in surveys and monitoring plots. Recent phylogenetic evidence supports classifying Tobusch fishhook cactus as Sclerocactus brevihamatus ssp. tobuschii. It is distinguished morphologically from its closest relative, S. brevihamatus ssp. brevihamatus, on the basis of yellow versus pink- or brown-tinged flowers, fewer radial spines, and fewer ribs. Additionally, subspecies tobuschii is endemic to limestone outcrops of the Edwards Plateau, while subspecies brevihamatus occurs in alluvial soils in the Tamaulipan Shrublands and Chihuahuan Desert. A recent investigation found genetic divergence between the two subspecies, although they may interact genetically in a narrow area where their ranges overlap. -
Journal of Seed Science
Online version: ISSN 2317-1545 JOURNAL OF SEED SCIENCE Continuation of the “Revista Brasileira de Sementes / Brazilian Seed Journal” 4 Issues a Year / Publicação Trimestral This Journal’s articles are indexed in: - BINAGRI / Ministério da Agricultura, Pecuária e Abastecimento www.agricultura.gov.br - SCIELO (Scientific Eletronic Library on line) www.scielo.br - SCOPUS - AGROBASE - AGRIS Coordenadoria Geral e Informação Documental Agrícola BINAGRI/MAPA - EBSCO Information Services Associação Brasileira de Tecnologia de Sementes Brazilian Association of Seed Technology JSS Viçosa, MG v. 40 n. 3 p.213-355 Sep. 2018 GENERAL INFORMATION The Journal of Seed Science (JSS) is the official publication of the Brazilian Association of Seed Technology, and publishes original articles and revisions in the field of Seed Science and Technology and related areas of the agricultural services. The JSS is a publication in continuation of the “Revista Brasileira de Sementes” (Brazilian Seed Journal), from January/2013. The mission of JSS is to publish scientific papers in the area of Seed Science and Technology, providing to the national and international agricultural sectors the knowledge for producing high quality seeds and the benefits from its use. Besides, JSS aims to contribute for the development and improvement of technologies that would aid in the economic and social improvement of the population, guaranteeing the basic input of the agricultural production and the preservation of the vegetable species. The JSS is published four times a year, although special numbers can be published. The Editorial Board is composed by one or more editors, Associated Editors and a Scientists Committee formed by scientists working with Seed Science and Technology. -
(12) United States Patent (10) Patent No.: US 6,271,001 B1 Clarke Et Al
USOO6271001B1 (12) United States Patent (10) Patent No.: US 6,271,001 B1 Clarke et al. (45) Date of Patent: Aug. 7, 2001 (54) CULTURED PLANT CELL GUMS FOR 56-164148 4/1983 (JP). FOOD, PHARMACEUTICAL COSMETIC 56-164149 4/1983 (JP). AND INDUSTRIAL APPLICATIONS 60-172927 2/1984 (JP). 61-209599 3/1985 (JP). 60-49604 9/1986 (JP). (75) Inventors: Adrienne Elizabeth Clarke, Parkville; 61-209599A 9/1986 (JP). Antony Bacic, Eaglemont; Alan 62-201594A 9/1987 (JP). Gordon Lane, Parramatta, all of (AU) 4053495 2/1992 (JP). 4-0997.42A 3/1992 (JP). (73) Assignee: Bio Polymers Pty. Ltd., Melbourne 5-070503A 3/1993 (JP) (AU) WO 88/06627 9/19ss (WO). WO 94/02113 2/1994 (WO). (*) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 OTHER PUBLICATIONS U.S.C. 154(b) by 0 days. Aspinall, G. and Molloy, J. (1969) Canadian J. Biochem. 47:1063-1070. (21) Appl. No.: 09/072,568 Bacic, A. et al. (1987) Australian J. Plant Physiol. (22) Filed: May 5, 1998 14:633-641. Bacic, A. et al. (1988) “Arabinogalactan proteins from Related U.S. Application Data stigmas of Nicotiana alata” Phytochem. 27(3):679-684. Barnoud et al. (1977) Physiol. Veg. 15:153–161. (63) Continuation-in-part of application No. 08/409,737, filed on Blumenkrantz, N. and Ashoe-Hansen, G. (1973) Anal. Bio Mar. 23, 1995, now Pat. No. 5,747.297. chem. 54:484-489. (51) Int. Cl." ....................................................... C12N 5/00 Baydoun et al. (1985) Planta 165(2):269-276 (abstract).