Effect of Using Lactobacillus Brevis IBRC-M10790 and Baker's Yeast
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Journal of Food Biosciences and Technology, Islamic Azad University, Science and Research Branch, Vol. 9, No. 1, 11-18, 2019 Effect of Using Lactobacillus Brevis IBRC-M10790 and Baker’s Yeast on Phytate Degradation of Sourdough and Barbari Bread H. Hadaegh a, S. M. Seyyedain Ardabili b*, M. Tajabadi Ebrahimi c, M. Chamani d, R. Azizi Nezhad e a Ph. D Student of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. b Associate Professor of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. c Associate Professor of the Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. d Professor of the Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. e Assistant Professor of the Plant Breeding Department, Science and Research Branch, Islamic Azad University, Tehran, Iran. Received: 10 September 2018 Accepted: 8 October 2018 ABSTRACT: The possible use of Lactobacillus brevis IBRC-M10790 with degrading phytase activity was investigated in sourdough and Barbari bread –using 20 and 30% of sourdough in the formulation- in order to enhance the nutritional bioavailability, and the efficiency of the bacteria in decreasing phytate via using in sourdough was compared to yeast. Results showed that L. brevis IBRC-M10790 has better efficiency in phytate reduction than yeast alone or the combination of yeast and bacteria, and can be effectively used for improving the nutritional properties of Barbari bread. Use of 30% sourdough in bread formulation gave the highest percentage of phytate reduction in breads. It seems that not only the percentage of sourdough (the amount of used yeast), but also fermentation time is important in reducing the level of phytate in Barbari bread. Statistical analysis revealed that pH is not the major factor for phytate reduction in Barbari breads with different percentages of sourdough. Keywords: Barbari Bread, Lactobacillus brevis, Phytate Reduction, Sourdough, Yeast. Introduction1 et al., 2015). Degradation of phytate occurs Phytic acid (myoinositol 1, 2, 3, 4, 5, 6- via the enzyme phytase (EC 3.1.3.8). This hexakisdihydrogen phosphate, phytate or enzyme can be derived from different IP6), is the major storage form of sources, such as animals, plants and phosphorous, comprising 1–5% by weight in microorganisms (De Angelis et al., 2003; cereals, legumes, oil seeds and nuts (Gupta Leenhardt et al., 2005; Matsuo et al., 2012). et al., 2015; Garcı́a-Estepa et al., 1999). It Considering fermented bakery products, has the ability to chelate multivalent metal phytase activity has been shown in different ions, especially zinc, calcium and iron, as strains of commercial baker`s yeast (Türk et well as resulting in poor bioavailability of al., 2000; Lambrechts et al., 1992), however, minerals (Garcı́a-Estepa et al., 1999; Gupta it seems not to be sufficient for the degradation of phytate. Thus, the addition of * phytase or micro-organisms producing Corresponding Author: [email protected] H. Hadaegh et al. phytase was proposed by various researchers direct effect on phytate degradation, and (Lopez et al., 2000; Sandberg and Andlid, they carry out their function by lowering pH, 2002; De Angelis et al., 2003; Reale et al., in order to provide favorable conditions for 2007; Palacios et al., 2008; Didar and endogenous cereal phytase. On the other Haddad Khodaparast, 2011). Nevertheless, hand, Palacios et al. (2008) stated that in the different reports have revealed that phytase presence of certain bifidobacterial strains, activity in lactic acid bacteria seems to be reducing pH is not the main factor for strain-specific (Reale et al., 2007; Lopez et hydrolyzing phytase. al., 2000). Several studies have been carried out on Bread made from wheat is a staple food the purification and characterization of in many countries, especially in Iran. Its phytase from different LAB or evaluating its consumption is 140-160 kg per year (more activity in various foods (Sumengen et al., than twice of what is consumed in Europe) 2013; Zamudio et al., 2001; Reale et al., (Abtahi et al., 2014). Since bread is 2007; De Angelis et al., 2003; Lopez et al., consumed at almost every meal, it is of 2000; Tang et al., 2010), however, this utmost importance in the case of nutrition study aims to use Lactobacillus brevis bioavailability. Thus, reducing phytate IBRC-M10790 in sourdough, to improve the should be given great attention. Among four nutritional value of bread by producing types of bread mainly consumed in Iran, phytase, and comparing its effect with yeast Barbari is the most popular one, and its taste on phytate degradation, as well as evaluating becomes unique when produced by the effect of pH for providing an optimum sourdough (Pourafshar et al., 2015). condition. To the best of our knowledge, this The use of sourdough in bread processing novel strain of LAB isolated from Tarhana has a long tradition and plays an important (Tafvizi and Tajabadi Ebrahimi, 2015), has role in the quality and nutritional value of not been used in any food matrix, for different breads (Diowksz and Kordialik- verifying its phytase production. Bogacka, 2017), especially in the case of IP6 reduction (Najafi et al., 2012; Poutanen et Materials and Methods al., 2009; Gobbetti et al., 2005; Gobbetti et - Materials al., 2014). In addition, using lactic acid The flour utilized in this study for bacteria (LAB) in sourdough has received measuring IP6 quantity, producing extensive attention, due to their impact on sourdough and Barbari bread was different characteristics of the dough and commercial-type wheat flour (Tehran bread. Phytase is one of the metabolites Bakhtar Co., Tehran, Iran) with an produced by some species of LAB (Reale et extraction rate of 79%. Flour characteristics al., 2007; De Angelis et al., 2003; Didar and are listed in Table 1. Salt was obtained from Haddad Khodaparast, 2011). According to the local market. A typical fresh baker`s different published reports, there is still a yeast (Saccharomyces cerevisiae) was challenge between the role of yeast and purchased from Iran Mayeh Co., Tabriz, LAB, regarding IP6 degradation. Leenhardt Iran. L. brevis IBRC-M10790, was used as a et al. (2005) illustrated the effect of dough sourdough starter, obtained from Tak Gen acidification in the phytate breakdown either Zist Co. (Tehran, Iran). by LAB or lactic acid. However, Reale et al. (2007) have reported that LAB do not have a 12 J. FBT, IAU, Vol. 9, No. 1, 11-18, 2019 Table 1. Chemical and rheological characteristics of the flour Characteristics Chemical Moisture (%) 14.18 Wet Gluten (%) 30.2 Protein (% of dry weight basis) 12.5 Ash (% of dry weight basis) 0.66 Rheological Farinograph water absorption (%) 54.1 Dough development time (min) 2.06 Dough stability (min) 18.03 Falling Numbers 364 Mixing properties of the flour were determined using a Farinograph according to AACC method (54.21 on 50.0 g of the flour) (AACC, 2000). Values represent the average of three replications. - Methods - Determination of pH - Starter Preparation pH measurements were carried out L. brevis IBRC-M10790 was received in according to AACC method (02-52) in a lyophilized form, and then grown in a triplicate order. sterile industrial broth culture for 12 h in a 37C incubator. Prior to this step, bacteria - Bread production were incubated for 5, 12, 24 and 48 h, and For the preparation of Barbari bread, 600 results showed that 12 h is the best time for g of flour, 10.8 g of salt, 24 g of fresh yeast, incubation, as some derivatives were and 420 ml of water were utilized (control consumed by the bacteria after this time. bread). For evaluating the effect of After incubation, all broth medium within sourdough on phytate reduction in bread, the cells were utilized in the preparation of Barbari samples were prepared using two sourdough with the cell density of 1.0 × 109 different amounts of sourdough: 20 g/100 g CFU/ml. of dough (formulation 1), and 30 g/100 g of dough (formulation 2). All ingredients were - Sourdough preparation mixed and kneaded in a spiral mixer Sourdoughs were prepared in a spiral (Diosna, model SP12-SP160, Germany) for mixer (Diosna Co., model SP12-SP160, 2 min slow speed and 6 min fast speed. The Germany), by mixing 500 g wheat flour with prepared dough was divided into 500 g 310 g tap water, 10 g salt, 20 g yeast (in the pieces and put into proofer (38◦C, RH=85%) trials which yeast was included) and the for 20 min. Thereafter, semi-proofed dough inoculum of the sourdough starter (1.0 × 109 pieces were brought out from the proofer, CFU/ml) for 2 min in slow speed and 6 min formed to the flattened shape by hands, fast speed. For the preparation of sourdough punched by fingers, and continued proofing starter, L. brevis IBRC-M10790 was for 6 min. Baking was carried out in a Miwe incubated for 12 h in MRS broth (De Man et rack oven (Germany) in 250C for 11 min. al., 1960) at 37C in an aerobic condition. Breads were cooled after 30 min and packed Prepared sourdoughs were thereafter put in in plastic bags for further analysis. the bowel, covered with a plastic cover and held for 24 h in room temperature. - Sample preparation Phytate was extracted from the samples 13 H. Hadaegh et al. based on the method described by Dost and as 300.494 ± 0.01 mg/ 100 g of dry weight Tokul (2006). The extracts were centrifuged basis. After 24 h fermentation in the control at 12000×g for 15 min (Micro 200R, Hettich sourdough (prepared by yeast), it decreased Co., UK), while the supernatants were to 121.272 ± 0.11 mg/ 100 g of dry weight filtered through a 0.22 μm filter (Sartorius basis (60% reduction) (P < 0.05).