Zhiyi Zuo, M.D., Ph.D.

Phone: (434) 982 4481 (work) or (434) 227-1007 (Cell) Address: Department of Anesthesiology, University of Virginia Health System, PO Box 800710, Charlottesville, VA 22908

ACADEMIC POSITIONS 10/1990 - 11/1990: Attending Physician of Anesthesiology Sun Yat-Sen University of Medical Sciences, Guangzhou, China. 07/2000 – 06/2003: Assistant Professor of Anesthesiology University of Virginia, Charlottesville, VA. 12/2002 – 06/2003: Assistant Professor of Neuroscience (secondary appointment) University of Virginia, Charlottesville, VA. 07/2003 – 06/2005: Associate Professor of Anesthesiology University of Virginia, Charlottesville, VA. 07/2003 – 06/2007: Associate Professor of Neuroscience (secondary appointment) University of Virginia, Charlottesville, VA. 04/2004 – 06/2005: Assistant Professor of Neurosurgery (secondary appointment) University of Virginia, Charlottesville, VA. 07/2005 – 06/2007: Associate Professor of Anesthesiology (tenured) University of Virginia, Charlottesville, VA. 07/2005 – 06/2007: Associate Professor of Neurosurgery (secondary appointment) University of Virginia, Charlottesville, VA. 07/2007 – present: Professor of Anesthesiology (without term) University of Virginia, Charlottesville, VA. 07/2007 – present: Professor of Neuroscience (secondary appointment) University of Virginia, Charlottesville, VA. 07/2007 – present: Professor of Neurosurgery (secondary appointment) University of Virginia, Charlottesville, VA. 07/2007 – present: Robert M. Epstein Professor (without term) University of Virginia, Charlottesville, VA

EDUCATION 09/1978 - 08/1983: M.D. Zhongshan Medical College, Guangzhou, China. 09/1985 - 08/1988: Master’s Degree of Anesthesiology Sun Yat-Sen University of Medical Sciences (formerly Zhongshan Medical College), Guangzhou, China. 12/1990 - 02/1994: Ph.D. Major: receptor physiology, pharmacology and biochemistry. University of Cambridge, Cambridge, UK.

CLINICAL TRAINING 09/1983 - 08/1985: General Surgery Resident Yichun Medical Academic School, Jiangxi, China. 09/1985 - 07/1989: Anesthesia Resident Sun Yat-Sen University of Medical Sciences, Guangzhou, China.

Zhiyi Zuo, MD, Ph.D. 1

08/1989 - 09/1990: Anesthesia Chief Resident Sun Yat-Sen University of Medical Sciences, Guangzhou, China. 07/1996 – 06/2000: Anesthesia Resident (combining program of internship and residency) Department of Anesthesiology, University of Virginia, Charlottesville, VA.

RESEARCH TRAINING 09/1985 - 08/1988: Master’s Degree Candidate Sun Yat-Sen University of Medical Sciences, Department of Anesthesiology, Guangzhou, China Research areas: physiological and pharmacological studies related to anesthesia 12/1990 - 02/1994: Ph.D. Student University of Cambridge, Physiological Laboratory, Cambridge, UK Research area: C-type natriuretic peptide receptors (particulate guanylyl cyclase). 03/1994 – 06/1996: Research Associate University of Virginia, Department of Anesthesiology, Charlottesville, USA Research area: anesthetics and the nitric oxide signaling pathway

ADDITIONAL TRAINING May 18 – 23, 2003: Financial management for non-financial managers. Darden Graduate School of Business Administration, University of Virginia, Charlottesville, USA Sept. 21 – 24, 2003: Strategic decision making. Darden Graduate School of Business Administration, University of Virginia, Charlottesville, USA 09/2018 – 12/2018: Leadership in Academic Matters, Office of the Executive Vice President and Provost, University of Virginia, Charlottesville, USA

LICENSURE AND CERTIFICATION Permanent ECFMG Certificate, 1995, Certificate No.: 0-528-243-9. Commonwealth of Virginia Board of Medicine, 1999, License No.: 0101221056. North Carolina Medical Board, 2005, License No.: 2005-00817 Diplomat of the American Board of Anesthesiology, April 26, 2002, No.: 35045

MAJOR COMMITTEE AND ADMINISTRATIVE ACTIVITIES 08/1988 – 11/1990: Director of Operating Room, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. 07/2004 – present: Vice Chair for Research/Research Committee Chair, Department of Anesthesiology, University of Virginia, USA. 2007 – present: Member, Promotion and Tenure Committee, Department of Anesthesiology, University of Virginia, USA. 2008 – 2011: Member, Scientific Advisory Committee, Association of University Anesthesiologists, USA.

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2010 – present: Academic Chair, Department of Anesthesiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. 2010 – 2014: Member, Scientific Advisory Committee, Society of Neurosurgical Anesthesia & Critical Care, USA. 2012 – 2015: Member, Geriatric Anesthesia Committee, American Society of Aneshesiologists, USA. 2012 – 2014: President: International Chinese Academy of Anesthesiology 2013 – present: Member, Neuroscience Abstract Review Committee, American Society of Aneshesiologists, USA. 2015 – present: Chair, Promotion and Tenure Committee, Department of Anesthesiology, University of Virginia, USA. 2017 – present: Member, Promotion and Tenure Committee, School of Medicine, University of Virginia, USA. 2018 – present: President: World Association of Chinese Anesthesiologists

GRANT SUPPORT 01/2001 – 12/2001: Hypoxic effects on glutamate transporters in rat neurons and glial cells. $20,279.8, Principal Investigator: Zhiyi Zuo Research and Development Committee, University of Virginia School of Medicine. 01/2001 – 12/2002: Inhalational anesthetics and glutamate transporters. $67,401.55, Principal Investigator: Zhiyi Zuo Foundation for Anesthesia Education and Research 07/2001 – 06/2003: The effects of reversed transport of glutamate on glutamate accumulation and neuronal survival under hypoxic and ischemic conditions. $132,000, Principal Investigator: Zhiyi Zuo American Heart Association-Mid-Atlantic Affiliate, Beginner Grant-in- Aid. 02/2003 – 01/2008: Volatile anesthetic modulation of glutamate transporters. RO1 GM065211 (year 1-5), $1,332,000 (total) Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 01/2004 - 12/2007: Neuroprotection after isoflurane preconditioning. RO1 NS045983, $1,163,246 (total), Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 07/2007 – 06/2013: Neuroprotection by RNA interference-produced autoregulation of inducible nitric oxide synthase expression Frontiers in Anesthesia Research Award, $500,000 Principal Investigator: Zhiyi Zuo International Anesthesia Research Society 07/2007 – 06/2009: Role of p38 mitogen-activated protein kinase in volatile anesthetic preconditioning-induced neuroprotection $132,000, Principal Investigator: Zhiyi Zuo American Heart Association-Mid-Atlantic Affiliate, Grant-in-Aid. 12/2007 – 11/2008: Volatile anesthetic modulation of glutamate transporter type 3 $99,691, Principal Investigator: Zhiyi Zuo

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Vice President for Research and Graduate Studies Office, University of Virginia 08/2008 – 04/2012: Volatile anesthetic modulation of glutamate transporters. RO1 GM065211 (year 6-9), $1,172,610, Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 09/2009 – 08//2011: Volatile anesthetic modulation of glutamate transporters. 3RO1 GM065211-S, $181,674, Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 07/2010 – 06/2013: Effects of oral hypoglycemics and dietary restriction on tau phosphorylation in diabetic mice 10GRNT3900019, $154,000, Principal Investigator: Zhiyi Zuo American Heart Association-Mid-Atlantic Affiliate, Grant-in-Aid. 04/2011 – 03/2012: Identification of regulatory factor X-regulated genes $6,300, Principal Investigator: Zhiyi Zuo Research and Development Committee, University of Virginia School of Medicine. 09/2012 – 08/2016: Volatile anesthetic effects on aging brain 1R01GM098308, $1,200,800, Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 02/2013 – 01/2014: Role of regulatory factor X1 in the human glioblastoma $61,648, Principal Investigator: Zhiyi Zuo Voices against brain tumor, USA 03/2014 – 02/2015: Anesthetic choice and amantadine on postoperative cognitive dysfunction $35,000, Principal Investigator: Zhiyi Zuo School of Medicine, Vice President for Research and Graduate Studies Office and department of Anesthesiology, University of Virginia 07/2014 – 06/ 2015: Environmental enrichment reduces postoperative cognitive dysfunction $45,000, Principal Investigator: Zhiyi Zuo Commonwealth of Virginia Alzheimer’s and Related Disease Research Fund. 08/2015 – 04/2017: Attenuation of postoperative cognitive dysfunction by amantadine 1R21 AG047472, $434,500, Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 09/2017 – 07/2022: Glial cell line-derived neurotropic factor and postoperative cognitive impairment in young rats R01HD089999, $1,674,700, Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 07/2017 – 04/2022: Photoacoustic Microscopy of the Awake Mouse Brain 1R01NS099261, $1,646,203, Principal Investigator: Song Hu (Role for Dr. Zuo: Co-Investigator, about 1/3 budget) National Institutes of Health, USA 09/2017 – 05/2019: Postoperative cognitive dysfunction and enriched environment 1R21AG056995, $434,500, Principal Investigator: Zhiyi Zuo National Institutes of Health, USA 04/2018 – 03/2023: Obesity-induced cerebral vascular remodeling and poor brain ischemic tolerance 1 R01 NS099118, $1,725,681, Principal Investigator: Zhiyi Zuo

Zhiyi Zuo, MD, Ph.D. 4

National Institutes of Health, USA

(Note: a K02 award from the National Institutes of Health, USA, had to be declined in 2004 due to the lack of my time for having two NIH R01 grants and one K02 grant simultaneously)

GRANT SUPPORT ON BEHALF OF MY TRAINEES 07/2010 – 06/2012: Haptoglobin polymorphism as a predicator of major adverse cardiac events and acute kidney injury after coronary artery bypass surgery in patients with diabetes 2010 Clinical Scholar Award, $80,000 Principal Investigator: Jacob Raphael, Mentor: Zhiyi Zuo International Anesthesia Research Society 07/2013 – 06/2014: Comparison of broad-band near-infrared spectroscopy-derived mitochondrial oxidation state and tissue oxygen saturation for the detection of ischemia in two models of rat cerebral ischemia 2013 Starter grant, $47,057 Principal Investigator: Robert H. Thiele, Mentor: Zhiyi Zuo Society of Cardiovascular Anesthesiology 05/2016 – 04/2020: Effect of sepsis on cytochrome aa3 oxidation state 1K08GM115861, $763,448 Principal Investigator: Robert H. Thiele, Mentor: Zhiyi Zuo National Institutes of Health, USA 08/2017 – 07/2021: Mechanisms of synaptic and behavioral dysfunction after postnatal anesthesia 1K08GM123321, $756,128 Principal Investigator: Nadia Lunardi, Mentor: Zhiyi Zuo National Institutes of Health, USA

NATIONAL AWARD ON BEHALF OF MY TRAINEES -2010: American Society of Aneshesiologists Resident Research Award-Third place, clinical studies with the study entitled “Patients with chronic pulmonary diseases stayed longer in hosptial after neurosurgery”. Winner : Narayana C. Varhabhatla, Mentor: Zhiyi Zuo -2013: American Society of Aneshesiologists Best Abstract-First prize, basic sciences with the study entitled “Amantadine alleviates postoperative cognitive dysfunction by reducing microglia-assocated neuroinflammation ans stimulating GDNF release in rats”. Winner: Junfeng Zhang, Mentor: Zhiyi Zuo

PARTICIPATION IN MULTICENTER STUDY 2000 – 2003: Sub-investigator (Principal Investigator: Michael M. Todd, NIH RO1NS038554): Intraoperative hypothermia for aneurysm surgery trial.

HONORS AND AWARDS 1. Excellent paper award in “The Second Medical Symposium of Guangzhou City” in 1988. 2. Excellent paper award in “The Third Symposium of Sun Yat-Sen University of Medical Sciences” in 1989. 3. Cambridge Overseas Research Student Award from 1990 to 1993. 4. Sir Run Run Show Cambridge Scholarship from 1990 to 1993.

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5. Third prize award in the 1997 Essay Contest for Residents sponsored by the American Society of Anesthesiologists, Inc. 6. New Investigator Award from the Foundation for Anesthesia Education and Research, 2001- 2002. 7. The American Society of Anesthesiologists’ Presidential Scholar Award, 2005 8. The Frontiers in Anesthesia Research Award, International Anesthesia Research Society, 2007 9. Member of the Foundation for Anesthesia Education and Research (FAER) Academy Mentors in Anesthesiology (elected in 2011). 10. Fok Ying-Tung Prize, the World Outstanding Chinese Doctor Award, 2018

SOCIETY MEMBERSHIP 1. American Society of Anesthesiologists. 2. International Anesthesia Research Society 3. Society for Neuroscience 4. Virginia State Medical Society 5. American Heart Association 6. Virginia Society of Anesthesiologists 7. American Society of Regional Anesthesia and Pain Medicine 8. Association of University Anesthesiologists (elected in 2003) 9. American Society for Biochemistry and Molecular Biology

GRANT REVIEW COMMITTEE 2003-2006: Regular member, American Heart Association-Mid-Atlantic Affiliate, USA 10/2004: Ad hoc, National Institutes of Health (SAT Study Section), USA 12/2004: Foreign guest reviewer for The Wellcome Trust, UK 06/2005: Ad hoc, National Institutes of Health (SAT Study Section), USA 10/2005: Ad hoc, National Institutes of Health (SAT Study Section), USA 06/2006: Ad hoc, National Institutes of Health (SAT Study Section), USA 07/2006 – 06/2010: Regular member, SAT Study Section, NIH, USA 10/2010 – present: Ad hoc, National Institutes of Health, USA

JOURNAL EDITORSHIP 01/2008 – 12/2013: Associate editor for Anesthesiology 01/2011 – present: Editorial board member for Medical Gas Research 12/2011 – present: Editor-in-Chief for World Journal of Anesthesiology 02/2013 – present: Academic editor for Plos One 01/2014 – present: Associate Editor-in-Chief for Journal of Anesthesia and Perioperative Medicine 01/2016 – present: Executive Editor-in-Chief for Translational Perioperative and Pain Medicine 06/2016 – present: Academic editor for Scientific Report

JOURNAL REVIEW Consulting reviewer for numerous journals including Annals of Surgery, Progress of Neurobiology, Molecular Pharmacology, Critical Care Medicine, Stroke, Journal of Cerebral Blood Flow and Metabolism, Anesthesiology, Anesthesia and Analgesia, Neuroscience, Experimental Neurology, Journal of Applied Physiology, Neuroscience Letters,

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Neuropharmacology, Biochimica et Biophysica Acta (BBA) - General Subjects, British Journal of Pharmacology, European Journal of Pharmacology, Cell and Tissue Research, Brain Research, Achieves of Medical Research and Current Neuropharmacology etc (more than 40 journals).

MENTORSHIP OF TRAINING PROGRAMS 2002 – present: Medical science training (MST) program of the University of Virginia. 2003 – present: Neuroscience graduate program of the University of Virginia.

SCIENTIFIC MEETING MODERATOR 2005: Experimental Neuroscience section, American Society of Anesthesiologists Annual Meeting, Atlanta, George, October, 2005. 2007: Experimental Neuroscience section, American Society of Anesthesiologists Annual Meeting, San Francisco, California, October, 2007. 2008: Ischemia: tolerance and preconditioning, 2008 Annual Meeting of Society for Neuroscience, Washington, DC, November, 2008. 2009: Ischemia and Neuroproection, 2009 Annual Meeting of American Society of Anesthesiologists, New Orleans, LA, October, 2010. 2010: Alzheimer’s disease, World Congress of NeuroTalk 2010, Singapore, Singapore, June 2010 2010: John D. Michenfelder New Investigator Award Oral Presentation, Society for Neuroscience in Anesthesiology and Critical Care, San Diego, CA, October, 2010. 2011: John D. Michenfelder New Investigator Award Oral Presentation, Society for Neuroscience in Anesthesiology and Critical Care, Chicago, IL, October, 2011. 2012: John D. Michenfelder New Investigator Award Oral Presentation, Society for Neuroscience in Anesthesiology and Critical Care, Chicago, IL, October, 2012.

LABORATORY TRAINEES Post-doctoral Fellows and Research Associates: (this section is not updated) Sang-Hwan Do, MD, Ph.D. –(2000-2001) Professor and Chairman, Seoul National University College of Medicine, Seoul, Republic of Korea. Yuanli Yu, MD – (2000) Research Specialist, Department of Internal Medicine, University of Virginia. Yueming Huang, MD, Ph.D. –(2000-2006) Research Associate, Department of Anesthesiology, University of Virginia. Hongyu Fang, MD, M.S. -(2001-2003) Research Associate, Department of Anesthesiology, University of Virginia. Shuqiu Zheng, MD, Ph.D. –(2001-2003) Research Associate, Department of Anesthesiology, University of Virginia. Hui-Bih Yuan, MD, M.S. – (2001-2002 and then 2004) Lecturer, Department of Anesthesiology and Pain Clinic, VGH-Taipei School of Medicine, National Yang-Ming University, Taiwan, R.O.C. Jue Li, MD, M.S. – (2001-2002) Lecturer, Department of Anesthesiology, Sun Yat-Sen University of Medical Sciences, Guangzhou, China. Yonghua Zhang, MD, M.S. – (2001-2003) Research Assistant, Department of Anesthesiology, University of Virginia. Young-Jin Lim, MD, Ph.D. – (2002-2003) Associate Professor, Seoul National University

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College of Medicine, Seoul, Republic of Korea. Ping, Zhao, MD, Ph.D. – (2002-present) Research Associate, Department of Anesthesiology, University of Virginia. Xiaorong Feng, MD, Ph.D. – (2003-2007) Instructor of Research, Department of Anesthesiology, University of Virginia. Gwanwoo Lee, MD, Ph.D. – (2003-2005) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Dankook University College of Medicine, Cheonan, Korea. Kaiwen Ma, MD, Ph.D. – (2003-2005) Research Associate, Department of Anesthesiology, University of Virginia. Ying Wang, MD, Ph.D. – (2004-2005) Post-doctoral Fellow, Department of Anesthesiology, University of Virginia. Chengbin Wang, MD, MS – (2005-2006) Visiting scholar, Department of Anesthesiology, University of Virginia and Assistant Professor, Department of Anesthesia, Beijing Anzhen Hospital, Capital University of Medical Science, Beijing, China. Longyun Peng, MD, Ph.D. – (2005-2006) Post-doctoral fellow, Department of Anesthesiology, University of Virginia. Liaoliao Li, MD, Ph.D. – (2006-2012) Post-doctoral fellow, Department of Anesthesiology, University of Virginia. Hee-Jung Baik, MD, Ph.D. (2006-2007) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Dong- Dae-Mun Hospital, School of Medicine, Ewha Woman’s University, Korea. Hae Hyuk Jung, MD, Ph.D. (2006-2007) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Internal Medicine, Kangwon National University Hospital, Kangwon National University, Korea Jeong Jin Lee, MD, Ph.D. (2006-2007) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Korea. Xuebing Xu, MD, Ph.D. (2006-2007) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Guangzhou Medical College, China. Jifeng Feng, MD (2007-2008) Visiting scholar, Department of Anesthesiology, University of Virginia and Director, Department of Anesthesiology and Intensive Care Unit, Guangxi Province’s Women and Children Hospital, Nanning China Soon Ae Lee, MD, Ph.D. (2007– 2008) Visiting scholar, Department of Anesthesiology, University of Virginia and Staff Anesthesiologist, Department of Anesthesiology and Pain Medicine, Center for live Cancer, National Cancer Center, Korea. Jie Ae Kim, MD, Ph.D. (2007-2008) Visiting scholar, Department of Anesthesiology, University of Virginia and Assistant Professor, Department of Anesthesiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Korea. Chenzhuo Feng, Ph.D. (2008-2013) Post-doctoral fellow, Department of Anesthesiology, University of Virginia. Lei Liu, MD (2008-2009) Post-doctoral fellow, Department of Anesthesiology, University of Virginia. Xing Wu, MD (2008-2009) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Cardiology, the 1st affiliated hospital of ZhongShan University, Guangzhou, China.

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Mi Sook Gwak, MD, Ph.D. (2008-2009) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Korea. Jiejie Li, MD (2008-2010) Ph.D. student, Department of Anesthesiology, University of Virginia and Department of Neurology, ZhongShan University, Guangzhou, China. Kyung-Hye Huh, Ph.D. (2008-2009) Post-doctoral fellow, Department of Anesthesiology, University of Virginia. Jun-Gwon Choi, MD, Ph.D. (2008-2009) Visiting scholar, Department of Anesthesiology, University of Virginia and Assistant Professor, Department of Anesthesia and Pain Medicine, Ilsan Dongguk University Hospital, Goyang City, Gyeonggi-Do, South Korea. Daowei Lin, MD (2009-present) Visiting scholar/postdoctoral fellow, Department of Anesthesiology, University of Virginia and Department of Anesthesiology, ZhongShan University, Guangzhou, China. Yueming Huang, MD, Ph.D. (2009-present) Research Associate, Department of Anesthesiology, University of Virginia. Hae-Jeong Jeong, MS, Ph.D. (2009-2010) Visiting scholar/postdoctoral fellow, Department of Anesthesiology, University of Virginia and Professor and Chairman, Department of Anesthesiology and Pain Medicine, National Cancer Center, Goyang-si, South Korea Su Nam Lee, MD, Ph.D. (2009-2010) Visiting scholar/postdoctoral fellow, Department of Anesthesiology, University of Virginia and Assistant Professor, Department of Anesthesiology, Korea Cancer Center Hospital, Seoul, South Korea Guofu Li, MD (2009-2010) Visiting scholar/postdoctoral fellow, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, China Sang-Hon Park, MD, Ph.D. (2010-2011) Visiting scholar/postdoctoral fellow, Department of Anesthesiology, University of Virginia and Assistant Professor, Department of Anesthesiology and Pain management, Seoul National University Bundang Hospital, Bundang-Gu Seongnam City, Korea. So-Young Lee, M.D. (2010-2011) Visiting scholar/postdoctoral fellow, Department of Anesthesiology, University of Virginia and Assistant Professor, Department of Anesthesiology, Korea Cancer Center Hospital (KIRAMS), Seoul, South Korea Lin Cao, MD, Ph.D. (2010-2011) Visiting scholar/postdoctoral fellow, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, The second Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdone, China Jiao Deng, MD (2010-2012) Ph.D. student, Department of Anesthesiology, University of Virginia and Department of Anesthesiology, Fourth Military Medical University, Xi’an, China. Jinbi Yin, MD, PhD (2011-2012) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China Hong Li, MD, PhD (2011-2012) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing, China Zhi Wang, MD, Ph.D. (2011-present) Visiting scholar/postdoctoral fellow, Department of

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Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, The second Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdone, China Jiangbei Cao, MD, Ph.D. (2011-2012) Visiting scholar, Department of Anesthesiology, University of Virginia and lecturer, Department of Anesthesiology, PLA General Hospital Beijing, P. R. China Xiaoling Zhu, MD (2011-2012) Visiting scholar, Department of Anesthesiology, University of Virginia and Lecturer, Department of Anesthesiology, Xi-Jing Hospital, Fourth Military Medical University, Xi’an, China Hai Yu, MD, Ph.D. (2011-2012) Visiting scholar, Department of Anesthesiology, University of Virginia and Lecturer, Department of Anesthesiology, Laboratory of Anesthesia & Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Jungyeon Yun, MD (2011-2012) Visiting scholar, Department of Anesthesiology, University of Virginia and lecturer, Department of Anesthesiology, Research Institute and Hospital, National Cancer Center, Gyeonggi-do, Republic of Korea Zhongxing Wang, MD (2012-2014) Ph.D. student, Department of Anesthesiology, University of Virginia and Department of Anesthesiology, The First Affiliated Hospital, Sun Yat- Sen University, Guangzhou, Guangdong, China Junfeng Zhang, MD (2012-2014) Ph.D. student, Department of Anesthesiology, University of Virginia and Attending Physician, Department of Anesthesiology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China Xiaoyan Ren, MD (2012-2013) Ph.D. student, Department of Anesthesiology, University of Virginia and Department of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning Province, China Hongying Tan, MD (2012-present) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Sun Yat-Sen University Cancer Center, Guangzhou, China Jun Li, MD, Ph.D. (2012-present) Visiting scholar, Department of Anesthesiology, University of Virginia and Lecturer, Department of Anesthesiology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, China Dan Fan, MD (2013-present) Visiting scholar, Department of Anesthesiology, University of Virginia and Attending Physician, Department of Anesthesiology, Sichuan Provincial People’s Hospital, Chengdu, China Li Wan, MD, Ph.D. (2013-2014) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China Weiran Shan, Ph.D. (2013-present) postdoctoral research fellow, Department of Anesthesiology, University of Virginia Bin Zheng, MD (2013-present) Visiting scholar, Department of Anesthesiology, University of Virginia and lecturer, Department of Anesthesiology, Guangzhou First Municipal Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China. Renchun Lai, MD, Ph.D. (2013-presnt) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Department of Anesthesiology, Sun Yat- Sen University Cancer Center, Guangzhou, China Lijun Cao, MD, Ph.D. (2013-presnt) Visiting scholar, Department of Anesthesiology,

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University of Virginia and Associate Professor, Second Xiangya Hospital, Central South University, Changsha, Hunan, China Yunzhen Wang, MD (2013-presnt) Ph.D. student, Department of Anesthesiology, University of Virginia and Department of Anesthesiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China Jiangjiang Bi, MD (2013-present) Visiting scholar, Department of Anesthesiology, University of Virginia and Lecturer, Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Lingli Gui, MD, Ph.D. (2014-present) Visiting scholar, Department of Anesthesiology, University of Virginia and Associate Professor, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China Gunn-Hee Kim, MD (2014-present) Visiting scholar, Department of Anesthesiology, University of Virginia and attending physician, Department of Anesthesia and Pain Medicine, National Medical Center, Seoul, Republic of Korea.

Other Trainees (under direct supervision for research) Undergraduates: Tracy Holbrook - (2002-2003) Undergraduate (majoring Biochemistry), University of Virginia. Nicole Briggs - (2003-2004) Undergraduate (majoring Psychology), University of Virginia. Paul Sang-Hwa - (2003-2004) Undergraduate (majoring Biochemistry), University of Virginia. Lawrence Gonzales - (2003-2004) Undergraduate (majoring Biochemistry), University of Virginia.

Medical Student: Tad Kim – (2004) Medical student, School of Medicine, University of Virginia. Sung Hyuk Im – (2006) Medical student, School of Medicine, University of Virginia. Allyson JA Morman – (2007) Medical student, School of Medicine, University of Virginia.

Anesthesiology Residents: Usha Barry, MD - (2003-2005) Resident, Department of Anesthesiology, University of Virginia. Ashley Shilling, MD - (2004-2005) Resident, Department of Anesthesiology, University of Virginia. William J. Mauermann, MD – (2004-2006) Resident, Department of Anesthesiology, University of Virginia. Komail Saifee, MD – (2006-2007) Resident, Department of Anesthesiology, University of Virginia. Robert H Thiele, MD – (2008-2011) Resident, Department of Anesthesiology, University of Virginia. Narayana C. Varhabhatla, MD – (2009-2013) Resident, Department of Anesthesiology, University of Virginia. Richard J. McMurtrey, MD – (2009-2010) Resident, Department of Anesthesiology, University of Virginia. Sunny Chiao, MD – (2013-presnt) Resident, Department of Anesthesiology, University of Virginia. Jason Maas, MD, Ph.D. – (2013-presnt) Resident, Department of Anesthesiology, University of Virginia. Zhiyi Zuo, MD, Ph.D. 11

Lauren G. Powlovich, MD – (2014 – present) Resident, Department of Anesthesiology, University of Virginia. Christopher Spence, MD – (2017 – present) Resident, Department of Anesthesiology, University of Virginia.

Faculty: Edward C. Nemergut, MD – (2002-present) Professor with tenure, Department of Anesthesiology, University of Virginia. Ashley Shilling, MD - (2005-present) Associate Professor, Department of Anesthesiology, University of Virginia. Jacob Raphael, MD – (2005-present) Professor, Department of Anesthesiology, University of Virginia. Robert H Thiele, MD – (2012-present) Associate Professor, Department of Anesthesiology, University of Virginia. Narayana C. Varhabhatla, MD (2013-presnt) Assistant Professor, Department of Anesthesiology, University of Virginia. Bhiken Naik, MD - (2013-presnt) Associate Professor, Department of Anesthesiology, University of Virginia. Nadia Lunardi, MD, Ph.D.- (2015-present), Assistant Professor, Department of Anesthesiology, University of Virginia. Lauren Dunn MD, Ph.D.- (2017-present), Assistant Professor, Department of Anesthesiology, University of Virginia.

CLINICAL TRAINEES Residents in the Department of Anesthesiology, particularly when they are in neuroanesthesia rotation.

TEACHING COURSES Director for the teaching block: history, statistics and research methods from 2005 to present.

JOURNAL CLUBS ORGANIZED FOR RESIDENTS Anesthesia research, May, 2004 Statistical thinking, September, 2004 Research, November, 2007

PATIENT CARE Providing general anesthesia service and subspecialty service (neuroanesthesia) to patients.

INVITED ORAL PRESENTATIONS/VISITING PROFESSOR (this section is not updated) 1. Cyclic guanosine monophosphate (cGMP) production stimulated in isolated rat glomeruli by natriuretic peptides. British Physiological Society Meeting, Manchester, UK, January, 1992. 2. Cyclic 3’5’ guanosine monophosphate (cGMP) is stimulated by C-type natriuretic peptide (CNP(1-22)) in the fetal telencephalon of rats. British Physiological Society Meeting, Oxford, UK, July, 1992. 3. Heterogeneity in the C-ANP binding proteins of rat renal glomeruli. British Physiological Society Meeting, Cambridge, UK. September, 1992.

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4. Effects of halothane on N-methyl-D-aspartate-induced cGMP changes in rat cerebellum: Localization and quantitative detection. Association of University Anesthesiologists Annual Meeting. San Diego, CA. May, 1995. 5. Localization and quantitative detection of cyclic guanosine 3,5-monophosphate changes caused by halothane or isoflurane in rat cerebellum. American Society of Anesthesiologists Annual Meeting, Atlanta, Georgia. October, 1995. 6. Inhalational anesthetics upregulate the expression of endothelial and inducible nitric oxide synthase. 5th International Meeting on the Biology of Nitric Oxide, Kyoto, Japan. September, 1997 (Invited Panelist). 7. The effects of inhalational anesthetics on nitric oxide-guanylyl cyclase signaling pathway. Department of Anesthesiology, University of California at Los Angels. December, 1999. 8. Volatile anesthetic effects on glutamate transporters. Department of Anesthesiology, University of Virginia. October, 2000. 9. Volatile anesthetics and nitric oxide-guanylyl cyclase signaling pathway. Invited lecture at the 165th Anniversary of Sun Yat-Sen University of Medical Sciences, Guangzhou, China. November, 2000 (Visiting Professor). 10. Volatile anesthetics and glutamate transporters. Invited lecture at the 165th Anniversary of Sun Yat-Sen University of Medical Sciences, Guangzhou, China. November, 2000 (Visiting Professor). 11. Cerebral physiology and the effects of anesthetics and techniques. Department of Anesthesiology, University of Virginia. May, 2001. 12. The effects of isoflurane and ischemic preconditioning on Purkinje neuron survival after an ischemic insult. American Society of Anesthesiologists Annual Meeting, New Orleans, LA. October, 2001. 13. Morphine preconditions Purkinje cells against cell death under in vitro simulated ischemia- reperfusion conditions. American Society of Anesthesiologists Annual Meeting, San Francisco, California. October, 2003. 14. Volatile anesthetic modulation of glutamate transporters. Presentation given to new graduate students of neuroscience program at the University of Virginia. November, 2003. 15. Neuroprotection after isoflurane preconditioning. Presentation given to new graduate students of neuroscience program at the University of Virginia. November, 2003. 16. Neuroprotection induced by anesthetics. Department of Anesthesiology, Ohio State University. May, 2004 (Visiting Professor). 17. Isoflurane preconditioning-induced neuroprotection. Department of Anesthesia, Stanford University. July, 2004. 18. Glutamate transporters: Why do I care? Department of Anesthesiology, University of Virginia, May, 2005. 19. Neuroprotection after isoflurane preconditioning. Department of Anesthesia and Perioperative Care, University of California San Francisco. January, 2006 (Visiting professor). 20. Glutamate transporters and volatile anesthetics. Preview of neuroscience research seminar series at the University of Virginia, March, 2006. 21. Anesthetic modulation of glutamate transporters. Neuroscience program research seminar series at the University of Virginia, March, 2006. 22. Neuroprotection after anesthetic preconditioning. Department of Anesthesia, University of Pennsylvania, March, 2006 (Visiting professor).

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23. Isoflurane preconditioning-induced neuroprotection. Department of Anesthesia, University of Iowa, January, 2007 (visiting professor). 24. Neuroprotection induced by anesthetics. Department of Anesthesia, University of Iowa, January, 2007 (visiting professor). 25. Neuroprotection induced by anesthetics. Department of Anesthesia, University of Virginia, April, 2007. 26. Volatile anesthetic preconditioning-induced neuroprotection. Invited panel speaker, American Society of Anesthesiologists Annual Meeting, San Francisco, October, 2007. 27. Anesthetic preconditioning- and postconditioning-induced neuroprotection. Department of Anesthesia, University of Utah. April, 2008 (visiting professor). 28. Anesthetic regulation of glutamate transporters. Invited panel speaker, Association of University Anesthesiologists Annual Meeting, Durham, North Carolina, May, 2008. 29. Neuroprotection induced by volatile anesthetic preconditioning and postconditioning. Invited speaker, Seoul National University College of Medicine, Seoul, Korea, June, 2008. 30. Anesthesiology resident training in the USA. Invited speaker, Seoul National University College of Medicine, Seoul, Korea, June, 2008. 31. How to get your work published and how to perform a meta-analysis? Invited speaker, Seoul National University Bundang Hospital, Bundang-gu, Korea, June, 2008 32. Anesthetic preconditioning- and postconditioning-induced neuroprotection and cardioprotection. Invited speaker, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China, June, 2008. 33. Anesthetic preconditioning- and postconditioning-induced neuroprotection. Invited speaker, Anesthesiologists Association, Guangdong Province, China, June, 2008. 34. Volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Invited speaker, Chinese American Society of Anesthesiologists Annual Meeting in conjunction with American Society of Anesthesiologists Annual Meeting, Orlando, FL, October, 2008. 35. Anesthetic-induced neuroprotection. Department of Anesthesiology, University of Medicine and Dentistry of New Jersey, November, 2008 (visiting professor). 36. Neuroprotection induced by anesthetic preconditioning and postconditioning. Invited speaker, Department of Anesthesiology, the Fourth Military Medical University, Xi’an, China, July, 2009. 37. Volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Invited speaker, the Fourth Meeting of the Anesthesiology Society of the East-north Three Provinces, Shenyang, China, July, 2009. 38. Anesthetics-induced neuroprotection. Department of Anesthesia, University of Virginia, January, 2010. 39. Anesthetics-induced neuroprotection. Invited speaker, Department of Anesthesiology, Texas Tech University, February, 2010. 40. Lidocaine attenuates isoflurane anesthesia-induced cognitive impairments in aged rats. Invited speaker, 2nd International workshop on anesthetic neurotoxicity, Toronto, Canada, June 2010. 41. Spine surgery under general anesthesia may not increase the risk for Alzheimer’s disease. Invited speaker, NeuroTalk 2010, Singapore, Singapore, June 2010. 42. Organ protective strategies studied in my laboratory. Invited speaker, Guangzhou, China, July 2010.

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43. Isoflurane and desflurane: are they equipotent to induce neuroprotection via preconditioning mechanism and to cause possible neurotoxicity? Invited speaker, Guangzhou, China, July 2010. 44. Volatile anesthetic preconditioning- and postconditioning-induced neuroprotection and role of glutamate transporter in neuroprotection. Invited speaker, Chendu, China, July 2010. 45. Volatile anesthetic preconditioning- and postconditioning-induced neuroprotection and possible neurotoxicity of these agents. Invited speaker, Xi’an, China, July 2010. 46. Volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Invited speaker, Harbin, China, July 2010. 47. Patient selection for ambulatory surgical center and use of anti-emetics in the setting. Invited speaker, Beijing, China, July 2010. 48. Hypothermia-induced neuroprotection. Invited speaker, Shanghain, China, March, 2011. 49. Anesthetic preconditioning- and postconditioning-induced neuroprotection. Invited speaker, 22nd Annual Spring Brain Conference, Tucson, Arizona, March, 2011 50. Age-dependent isoflurane effects on rat brains. Invited speaker, Xi’an, China, April, 2011. 51. How to get your work published? Invited speaker, Xi’an, China, April, 2011. 52. Cerebral physiology and the effects of anesthetics and techniques. Department of Anesthesiology, University of Virginia. April, 2011. 53. Using CONSORT and STROBE to guide your paper writing. Invited speaker, Xi’an, China, April, 2011. 54. Glutamate transporter type 3, its role in ischemic brain injury and a story branched out from this research, Grand round, NIH Clinical Center, Washington, DC, January, 2012. 55. Volatile anesthetic preconditioning and postconditioning induce neuroprotection in adult and neonatal rats. Symposium organizer and speaker, International Stroke Conference 2012, New Orleans, Louisiana, February, 2012 56. Mechanisms for volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Xiangya Hospital, Changsha, China, April 5, 2012 57. How to get your research work published, Third Xiangya Hospital, Changsha, China, April 6, 2012 58. Thinking outside the box: another responsibility for anesthesiologists, Keynote speak, Annual Meeting of Chinese Anesthesiologists Association, Guangzhou, China, April 7, 2012 59. Mechanisms for volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Annual Meeting of Chinese Anesthesiologists Association, Guangzhou, China, April 8, 2012 60. How to get your research work published, Annual Meeting of Chinese Anesthesiologists Association, Guangzhou, China, April 8, 2012 61. Mechanisms for volatile anesthetic preconditioning-induced neuroprotection. Xijing Hospital, Xi’an, China, April 9, 2012 62. Mechanisms for volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Tangdu Hospital, April 10, 2012. 63. Glutamate transporter type 3, its role in ischemic brain injury and a story branched out from this research, Grand round, Department of Neurosurgery, University of Virginia, August, 2012. 64. Mechanics for getting your work published. First Affiliated Hospital, China Medical University, Shenyang, China, Oct 31, 2012. 65. Mechanics for getting your work published. Second Affiliated Hospital, China Medical University, Shenyang, China, Nov 1, 2012.

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66. Mechanisms for volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Second Affiliated Hospital, China Medical University, Shenyang, China, Nov 1, 2012. 67. Mechanics for getting your work published. Beijing Renming Hospital, Beijing, China, Nov 2, 2012. 68. Mechanics for getting your work published. Jishuipang Hospital, Beijing, China, Nov 2, 2012 69. Mechanisms for volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. 301 Hospital, Beijing, Beijing, China, Nov 4, 2012. 70. Mechanics for getting your work published. Huqiao Hospital, Guangzhou, China, Nov 7, 2012. 71. Mechanics for getting your work published. Renji Hospital, Beijing, China, Nov 9, 2012. 72. Mechanisms for volatile anesthetic preconditioning- and postconditioning-induced neuroprotection. Children’s Hospital, Fudan University, Shanghai, China, Nov 11, 2012. 73. Volatile anesthetic use and the preconditioning and postconditioning effects of these anesthetics in the brain. Tumor Hospital, Sun Yat-sen University, Guangzhou, China, March 18, 2013 74. Volatile anesthetic use and the preconditioning and postconditioning effects of these anesthetics in the brain. Xiehe Hospital and Wuhan anesthesiologist Association meeting, Wuhan, China, March 20, 2013. 75. Mechanics for getting your work published. Xiehe Hospital and Wuhan anesthesiologist Association meeting, Wuhan, China, March 20, 2013. 76. Postoperative cognitive dysfunction and the effects of anesthesia and surgery on Alzheimer’s disease development: an update, Grand round/visiting professor, State University of New York at Stony Brook, New York, April 3, 2013 77. My glutamate transporter story, University of Pennsylvania Hospital, April 11, 2013 78. Mechanics for getting your work published. The First Affiliated Hospital, Fujian Medical University, Fuzhou, China, May 16, 2013 79. Mechanics for getting your work published. Annual Meeting of Association of Anesthesiologists from Huadong Six Provinces and One City, Xiamen, China, May 17, 2013 80. Volatile anesthetic postconditioning-induced neuroprotection, Annual Meeting of Association of Anesthesiologists from Huadong Six Provinces and One City, Xiamen, China, May 17, 2013 81. Anesthetic management for intracranial vascular surgery. 2013 TianTang-International Symposium for Neuroanesthesia, Beijing, May 18, 2013 82. Volatile anesthetic postconditioning-induced neuroprotection, 2013 TianTang-International Symposium for Neuroanesthesia, Beijing, May 18, 2013 83. History of large animal stroke models and clinical translation, Pangu Stroke Meeting, Shanghai, China, May 20, 2013. 84. Research hot topics of volatile anesthetics and volatile anesthetic postconditioning-induced neuroprotection, Renji Hospital, Shanghai, China, May 22, 2013 85. Research hot topics of volatile anesthetics and volatile anesthetic postconditioning-induced neuroprotection, Symposium of the Association of Anesthesiologists in Zhejiang Province, Hangzhou, China, May 22, 2013 86. My glutamate transporter story, Xijing Hospital, Xi’an, China, may 23, 2013

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87. Research hot topics of volatile anesthetics and volatile anesthetic postconditioning-induced neuroprotection, The Fifth International Symposium of Anesthesia Basic Science and Clinical Research, May 25, 2013 88. Volatile anesthetics-induced neuroinflammation and anti-inflammatory effects, The Fifth International Symposium of Anesthesia Basic Science and Clinical Research, May 25, 2013 89. My glutamate transporter story, Second Affiliated Hospital, Harbin Medical University, Harbin, China, May 27, 2013 90. Future of Clinical Anesthesia, Second Affiliated Hospital, Harbin Medical University, Harbin, China, May 28, 2013 91. Mechanics for getting your work published, Second Affiliated Hospital, Harbin Medical University, Harbin, China, May 28, 2013 92. Research hot topics of volatile anesthetics and volatile anesthetic postconditioning-induced neuroprotection, Symposium of the Association of Anesthesiologists in Heilongjian Province, Harbin, China, May 28, 2013 93. Research hot topics of volatile anesthetics and volatile anesthetic postconditioning-induced neuroprotection, Symposium of the Association of Anesthesiologists in Beijing City, Beijing, China, May 29, 2013 94. Mechanics for getting your work published, Beijing Chanyang Hospital, Beijing, China, May 30, 2013 95. Postoperative cognitive dysfunction and the effects of anesthesia and surgery on Alzheimer’s disease development: an update, Grand round/visiting professor, Thomas Jefferson University, Philadelphia, PA, March 7, 2014 96. Role of anesthetics in neuroinflammation after surgery, Oral presentation as a panelist, Annual Meeting of Internal Anesthesia Research Society, Montréal, Canada, May 19, 2014.

Research Seminars Presented by My Associates and Me in the Department of Anesthesiology, University of Virginia (this section is not fully updated) 1. S-H Do and Z Zuo: Effects of intravenous and local anesthetics on glutamate transporter type 3 activity. September, 2001. 2. H Fang and Z Zuo: Volatile anesthetic effects on glutamate transporter type 2 and its mutant. February, 2002. 3. Y Huang and Z Zuo: Effects of volatile anesthetics on EAAT3 trafficking and expression. May, 2002. 4. S Zheng and Z Zuo: Isoflurane preconditioning-induced neuroprotection. October, 2002. 5. H Fang and Z Zuo: Glutamate transporter type 3 attenuates the activation of NMDA receptors co-expressed in Xenopus oocytes. January, 2003. 6. H Fang and Z Zuo: PMA modulates the glutamate-gated chloride conductance in rat glutamate transporter type 4. October, 2003. 7. P Zhao and Z Zuo: Isoflurane preconditioning induces inducible nitric oxide synthase- dependent neuroprotection in the neonatal rats. November, 2003. 8. Y Huang and Z Zuo: Isoflurane induces a protein kinase C alpha-dependent increase in cell surface protein level and activity of glutamate transporter type 3 in C6 glioma cells. February, 2004. 9. X Feng and Z Zuo: Allele-specific Silencing of Alzheimer’s Disease Genes. September, 2004. 10. K Ma and Z Zuo: Specific modification of the expression of the glutamate transporter

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EAAT3 by the transcription factor. December, 2004. 11. P Zhao, Y Huang and Z Zuo: Morphine and Tan-67 preconditioning-induced neuroprotection. May, 2005. 12. X Feng and Z Zuo: Specific silencing of BACE1 expression and Alzheimer’s disease. November, 2005. 13. K. Ma and Z Zuo: Transcription factor RFX1 increases the expression of neuronal glutamate transporter type 3. April, 2006. 14. P Zhao, L Li and Z Zuo: Is isoflurane preconditioning-induced neuroprotection long-lasting? November, 2006. 15. X Xu, X Feng and Z Zuo: Targeting inducible nitric oxide synthase for protection in macrophage: the effects of isoflurane preconditioning and RNA interference. March, 2007. 16. L Li and Z Zuo: Isoflurane preconditioning increases Bcl-2 expression and reduces cytochrome c release from the mitochondria in the ischemic penumbra of rat brain. Department of Anesthesia, University of Virginia, November, 2007. 17. L Li and Z Zuo: Isoflurane preconditioning improved long-term neurological outcome in adult rats after transient focal cerebral ischemia. November, 2008. 18. C Feng and Z Zuo: RFX1 knockout, a journey. May, 2009. 19. J Li and Z Zuo: Pyrrolidine dithiocarbamate-induced neuroprotection. November, 2010. 20. C Feng and Z Zuo: RFX1 regulation of transforming growth factor beta 2 (TGFβ2) expression and cell proliferation in human neuroblastoma cells. November, 2011. 21. J Deng and Z Zuo: The effects of Actos on cognitive functions and neuropathological changes of diabetic mice. November, 2012.

PATENT 1. Z Zuo: Preactivation of delta-1 opioid receptors for neuroprotection. US provisional patent number: 60/652,640, granted in 2005. 2. Z Zuo: Endotracheal tube. US provisional patent number: 60/923,326, granted in 2007. 3. Z Zuo: Compositions and Methods for Improving Cognitive and Neurological Functions. US provisional patent number: 61/414,051, granted in 2010. 4. Z Zuo: Compositions and Methods for Preventing and Treating Hypoxic and Ischemic Brain Injury. US provisional patent number: 61/698,932, granted in 2012.

PUBLICATIONS Full publications in Peer-reviewed English Journals 1. J Brown and Z Zuo: Renal receptors for atrial and C-type natriuretic peptides in the rat. Am. J. Physiol. 263:F89-F96, 1992. 2. J Brown and Z Zuo: C-type natriuretic peptide and atrial natriuretic peptide receptors of rat brain. Am. J. Physiol. 264:R513-R523, 1993. 3. J Brown and Z Zuo: Receptor proteins and biological effects of C-type natriuretic peptides in the glomerulus of the rat. Am. J. Physiol. 266:R1383-R1394, 1994. 4. J Brown and Z Zuo: Natriuretic peptide receptors are expressed during cerebral growth in the fetal rat. Am. J. Physiol. 269:R261-R273, 1995. 5. J Brown and Z Zuo: Natriuretic peptide receptors in the fetal rat. Am. J. Physiol. 269:E253- E268, 1995. 6. Z Zuo and R A Johns: Halothane, enflurane, and isoflurane do not affect the basal or

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agonist-stimulated activity of particulate guanylyl cyclases of rat brain. Anesthesiology 83: 395-404,1995. 7. Z Zuo, J De Vente and RA Johns: Halothane and isoflurane dose-dependently inhibit the cyclic GMP increase caused by N-methyl-D-aspartate in rat cerebellum: Novel localization and quantitation by in vitro autoradiography. Neuroscience 74:1069- 1075, 1996. 8. Z Zuo, A Tichotsky and RA Johns: Halothane and isoflurane inhibit vasodilation due to constitutive but not inducible nitric oxide synthase. Anesthesiology 84:1156- 1165, 1996. 9. Z Zuo and RA Johns: Inhalational anesthetics up-regulate constitutive and Lipopolysaccharide-induced inducible nitric oxide synthase expression and activity. Molecular Pharmacology 52: 606-612, 1997. 10. Z Zuo, A Tichotsky and RA Johns: Inhibition of excitatory neurotransmitter-nitric oxide signaling pathway by inhalational anesthetics. Neuroscience 93:1167-72, 1999. 11. Z Zuo: Isoflurane enhances glutamate uptake via glutamate transporters in rat glial cells. NeuroReport 12:1077-1080, 2001. 12. S-H Do, GL Kamatchi, JM Washington and Z Zuo: Effects of volatile anesthetics on glutamate transporter, excitatory amino acid transporter type 3. Anesthesiology 96:1492- 7, 2002. 13. S-H Do, H Fang, B-M Ham and Z Zuo: The effects of lidocaine on the activity of glutamate transporter, EAAT3: The role of protein kinase C and phosphatidylinostol 3-kinase. Anesthesia and Analgesia 95:1263-8 2002. 14. H Fang, Y Huang and Z Zuo: The different responses of rat glutamate transporter type 2 and its mutant (tyrosine 403 to histidine) activity to volatile anesthetics and activation of protein kinase C. Brain Research 953:255-264, 2002. 15. J Li, S Zheng and Z Zuo: Isoflurane decreases AMPA-induced dark cell degeneration and edematous damage of Purkinje neurons. Brain Research 958:399-404, 2002. 16. S Zheng and Z Zuo: Isoflurane preconditioning reduces Purkinje cell death in an in vitro model of rat cerebellar ischemia. Neuroscience, 118:99-106, 2003. 17. S-H Do, B-M Ham and Z Zuo: Effects of propofol on the activity of glutamate transporter, EAAT3: The role of protein kinase C and phosphatidylinostol 3-kinase. Neuroscience Lett., 343:113-116, 2003. 18. Y J Lim, Y Huang, H Fang and Z Zuo: Effects of Local and Intravenous Anesthetics on the Activity of Glutamate Transporter Type 2. Brain Research Bulletin 61:537-40, 2003. 19. Y Huang and Z Zuo: Isoflurane enhances the expression and activity of glutamate transporter type 3 in C6 glioma cells. Anesthesiology 99:1346-53, 2003. 20. J-H Kim, Y-J Lim, Y-J Ro, S-W Min, C-S Kim, S-H Do, Y-L Kim and Z Zuo: Effects of ethanol on the rat glutamate transporter EAAT3 expressed in Xenopus oocytes: role of protein kinase C and phosphatidylinositol 3-kinase. Alcoholism: Clinical & Experimental Research 27:1548-53, 2003. 21. H-B Yuan, Y Huang, S Zheng and Z Zuo: Hypothermic preconditioning increases survival of Purkinje neurons in rat cerebellar slices after an in vitro simulated ischemia. Anesthesiology 100:331-7, 2004. 22. Y-J Lim, S Zheng and Z Zuo: Morphine preconditions Purkinje cells against cell death under in vitro simulated ischemia-reperfusion conditions. Anesthesiology 100:562-8, 2004. 23. P Zhao and Z Zuo: Isoflurane preconditioning induces neuroprotection that may be

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inducible nitric oxide synthase-dependent in the neonatal rats. Anesthesiology 101:695- 702, 2004. 24. S Zheng and Z Zuo: Isoflurane preconditioning induces neuroprotection against ischemia via activation of p38 mitogen-activated protein kinases. Molecular Pharmacology 65:1172- 1180, 2004. 25. U Barry and Z Zuo: Opioids: Old Drugs for Potential New Applications. Current Pharmaceutical Design 11:1343-1350, 2005. 26. Z Zuo: Role of opioid receptor internalization and -arrestins in the development of opioid tolerance. Anesthesia and Analgesia 101:728-34, 2005. 27. EC Nemergut, Z Zuo, JA Jane, Jr., and ER Laws, Jr.: Predictors of diabetes insipidus after transsphenoidal surgery: A review of 881 patients. Journal of Neurosurgery 103:448-454, 2005. 28. MM Todd, BJ Hindman, WR Clarke, JC Torner, ...... Z Zuo (I am one of the many investigators)...... : Mild intraoperative hypothermia during surgery for intracranial aneurysm. New England Journal of Medicine 352:135-145, 2005. 29. Y Huang and Z Zuo: Isoflurane induces a protein kinase C alpha-dependent increase in cell surface protein level and activity of glutamate transporter type 3. Molecular Pharmacology 67:1522-1533, 2005. 30. Y Huang and Z Zuo: The effects of isoflurane on oxygen-glucose deprivation-induced changes of gene expression profiling in glial-neuronal co-cultures. Acta Anaesthesiologica Scandinavica 49:1544-51, 2005. 31. Z Zuo and H Fang: Glutamate transporter type 3 attenuates the activation of N-methy-D- aspartate receptors co-expressed in Xenopus Oocytes. Journal of Experimental Biology 208 (11):2063-2070, 2005. 32. Y.Y. Huang, Z. Zuo, H.B. Yuan, M.Y. Tsou, M.T. Chen and S.K. Tsai: A Paraspinal Abscess Developed after Epidural Analgesia in an Obstetric Patient. International Journal of Obstetric Anesthesia 14:252-255, 2005. 33. P Zhao and Z Zuo: Prenatal hypoxia-induced adaptation and neuroprotection that is inducible nitric oxide synthase-dependent. Neurobiology of Disease 20:871-880, 2005. 34. Z Zheng and Z Zuo: Isoflurane preconditioning decreases glutamate receptor overactivation-induced Purkinje neuronal injury in rat cerebellar slices. Brain Research 1054:143-151, 2005. 35. GW Lee, Y Huang, JM. Washington, N Briggs and Z Zuo: Carbamazepine enhances the activity of glutamate transporter type 3 via phosphatidylinositol 3-kinase. Epilepsy Research 66:145-53, 2005. 36. EC Nemergut and Z Zuo: Airway Management in Patients with Pituitary Disease: A Review of 746 Patients. J Neurosurg Anesth 18(1):73-7, 2006. 37. J-H Kim, S-H Do, Y-L Kim and Z Zuo: Effects of Chronic Exposure to Ethanol on Glutamate Transporter EAAT3 expressed in Xenopus Oocytes: Evidence for Protein Kinase C Involvement. Alcoholism: Clinical & Experimental Research 29(11):2046-52, 2005. 38. X Feng, P Zhao, Y He and Z Zuo: Allele-specific silencing of Alzheimer’s disease genes: The amyloid precursor protein genes with Swedish or London mutations. Gene 371:68- 74, 2006. 39. J-Y Yun, J-H Kim, H-K Kim, S-H Do, Y-J Lim and Z Zuo: Effects of intravenous anesthetics on the activity of the rat glutamate transporter EAAT3 expressed in Xenopus oocytes. European Journal of Pharmacology 531:133-9, 2006.

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40. H Fang, Y Huang and Z Zuo: Enhancement of substrate-gated chloride currents via rat glutamate transporter EAAT4 by phorbol-12-myrisate-13-acetate. Am J Physiol-Cell Ph. 290:C1334-40, 2006. 41. J Raphael, S Abedat, J Rivo, K Meir, R Beeri, T Pugatsch, Z Zuo and YGozal: Volatile Anesthetics Attenuate Myocardial Apoptosis in Rabbits after Regional Ischemia and Reperfusion via Akt Signaling and Modulation of Bcl-2 Family Proteins. J Pharmacol Exp Ther. 318:186-94, 2006. 42. S Cechova and Z Zuo: Inhibition of glutamate transporters increases the minimum alveolar anesthetic concentration for isoflurane in rats. Br J Anaesth 97:192-5, 2006. 43. WJ. Mauermann, AM Shilling and Z Zuo: A Comparison of Neuraxial block Versus General Anesthesia for Elective Total Hip Replacement: A Meta-Analysis. Anesthesia &Analgesia 103:1018-25, 2006. 44. Z Zuo, Y Wang and Y Huang: Isoflurane preconditioning protects human neuroblastoma SH-SY5Y cells against in vitro simulated ischemia-reperfusion through the activation of extracellular signal-regulated kinases pathway European Journal of Pharmacology 542:84-91, 2006. 45. K Ma, S Zheng and Z Zuo: Transcription factor RFX1 specifically increases the expression of neuronal glutamate transporter type 3. J Biol Chem 281(30):21250-5, 2006. 46. H-B Yuan, Y Huang and Z Zuo: Hypothermic Preconditioning Reduces Purkinje Cell Death by Preventing the Over-expression of Inducible Nitric Oxide Synthase in Rat Cerebellar Slices after an In Vitro Simulated Ischemia. Neuroscience 142:381-9, 2006. 47. P Zhao, Y Huang and Z Zuo: Opioid preconditioning induces an opioid receptor-dependent delayed neuroprotection against ischemia in rats. J. Neuropath Exp Neur. 65:945-52, 2006. 48. Y Huang, X Feng, JJ Sando and Z Zuo: Critical role of serine 465 in isoflurane-induced increase of cell-surface redistribution and activity of glutamate transporter type 3. J Biol Chem 281(50):38133-8, 2006. 49. X Gao, L Peng, C.M Adhikari, L Jun and Z Zuo: Spironolactone reduced arrhythmia and maintained magnesium homeostasis in patients with congestive heart failure. J Cardiac Failure 13(3):170-7, 2007. 50. C Zuo and Z Zuo: The relationship between brain size and intelligence in human. Res. J Med. Sciences 1:70-73, 2007. 51. JY Yun, JH Kim, SH Do and Z Zuo: Propofol reverses oxidative stress-reduced activity of glutamate transporter EAAT3: the evidence for protein kinase C involvement. Eur J. Pharmacol 565(1-3):83-8, 2007. 52. C Wang, JJ Lee, HH Jung and Z Zuo: Pretreatment with volatile anesthetics, but not with the nonimmobilizer 1,2-dichlorohexafluorocyclobutane reduced cell injury in rat cerebellar slices after an in vitro simulated ischemia. Brain Research 1152:201-8, 2007. 53. P Zhao, L Peng, L Li, X Xu and Z Zuo: Isoflurane preconditioning improves long-term neurological outcome after ischemia. Anesthesiology 107:963-70, 2007. 54. H.B Yuan, Z Zuo, K.W Yu, W.M Lin, H.C Lee and K.H Chan: Bacterial Colonization of Epidural Catheters Used for Postoperative Analgesia: Microbiological Examination and Risk Factors Analysis. Anesthesiology 108:130-137, 2008. 55. X Xu, J Feng and Z Zuo: Isoflurane preconditioning reduces the rat NR8383 macrophage injury induced by lipopolysaccharide and interferon-γ. Anesthesiology 108:643-50, 2008. 56. J Raphael, Z Zuo, S Abedat, R Beeri and Y Gozal: Isoflurane preconditioning decreases

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myocardial infarction in rabbits via up-regulation of hypoxia inducible factor 1 that is mTOR mediated. Anesthesiology 108:415-25, 2008. 57. JJ Lee, L Li, HH Jung and Z Zuo: Postconditioning with isoflurane reduced brain injury in rat corticostriatal slices after an in vitro simulated ischemia. Anesthesiology 108:1055-62, 2008. 58. HY Park, JH Kim, Z Zuo and SH Do: Ethanol increases the activity of rat excitatory amino acid transporter type 4 expressed in Xenopus oocytes : role of protein kinase C and phosphatidylinositol 3-kinase. Alcoholism: Clinical & Experimental Research 32:348- 354, 2008. 59. L Li, L Peng and Z Zuo: Isoflurane preconditioning increases Bcl-2 expression and reduces cytochrome c release from the mitochondria in the ischemic penumbra of rat brain. Eur J. Pharmacol. 586:106-13, 2008. 60. Y Zhai, X Gao, Q Wu, L Peng, J Lin, Z Zuo: Fluvastatin decreases cardiac fibrosis possibly through regulation of TGF-β1/Smad 7 expression in the spontaneously hypertensive rats. Eur J. Pharmacol. 587:196-203, 2008. 61. SY Yoo, JH Kim, SH Do and Z Zuo: Inhibition of the activity of excitatory amino acid transporter 4 expressed in Xenopus Oocytes after chronic exposure to ethanol. Alcoholism: Clinical & Experimental Research. 32:1292-1298, 2008. 62. X Xu and Z Zuo: Isoflurane preconditioning reduces mouse microglia injury induced by lipopolysaccharide and interferon-γ. Neuroscience 154;1002-1008, 2008. 63. HH Jung, JJ Lee and Z Zuo: Inability of volatile anesthetics to inhibit oxygen-glucose deprivation-induced glutamate release via glutamate transporters and anion channels in rat corticostriatal slices. Brain Research. 1227:234-9, 2008. 64. HJ Baik, Y Huang, JM. Washington and Z Zuo: Critical role of S465 in protein kinase C increased rat glutamate transporter type 3 activity. Intern J Neurosci 119(9):1419-1428, 2009. 65. RH Thiele, N Pouratian, Z Zuo, DC Scalzo, HA Dobbs, AS Dumont, NF Kassell, EC Nemergut: Strict Glucose Control Does Not Affect Mortality and Increases the Incidence of Vasospasm in Aneurysmal Subarachnoid Hemorrhage Patients. Anesthesiology 110:603-10, 2009. 66. J Ryu, I-Y Cheong, SH Do, Z Zuo: Alphaxalone, a neurosteroid anaesthetic, increases the activity of the glutamate transporter type 3 expressed in Xenopus oocytes. Eur J Pharmacology 602:23-7, 2009. 67. D Baranov, PE Bickler, GJ Crosby, DJ Culley, MF Eckenhoff, RG Eckenhoff, KJ Hogan, V Jevtovic-Todorovic, A Palotas, M Perouansky, E Planel, JH silverstein, H Wei, RA Whittington, Z Xie and Z Zuo: Consensus statement: first international workshop on anesthetics and Alzheimer’s disease. Anesthesia and Analgesia 108:1627-30, 2009. 68. X Gao, X He, B Luo, L Peng, J Lin and Z Zuo: Angiotensin II increases collagen I expression via transforming growth factor-beta1 and extracellular signal-regulated kinase in cardiac fibroblasts. Eur J Pharmacol. 606:115-20, 2009. 69. HJ Baik, SA Lee, JM. Washington and Z Zuo: Amitriptyline inhibits the activity of the rat glutamate transporter EAAT3 expressed in Xenopus oocytes. J Pharma Pharmacol. 61:577-81, 2009. 70. JA Kim, L Li and Z Zuo: Delayed treatment with isoflurane attenuates lipopolysaccharide and interferon γ-induced activation and injury of mouse microglial cells. Anesthesiology 111:566-73, 2009. 71. JA Kim, L Li and Z Zuo: Isoflurane, but not desflurane, induces a postconditioning effect on

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bovine pulmonary arterial endothelial cells exposed to oxygen-glucose deprivation. Eur J Pharmacol. 615:144-9, 2009. 72. SA Lee, JW Choi and Z Zuo: Volatile anesthetics attenuate oxidative stress-reduced activity of glutamate transporter type 3. Anesthesia and Analgesia. 109: 1506-10, 2009. 73. C Feng, W Xu and Z Zuo: Knockout of the regulatory factor X1 gene leads to early embryonic lethality. Biochem Bioph Res Co. 386:715-7, 2009. 74. L Li and Z Zuo: Isoflurane preconditioning improves short-term and long-term neurological outcome after focal brain ischemia in adult rats. Neuroscience 164:497-506, 2009. 75. X Gao, CM Adhikari, L Peng, X Guo, Y Zhai, X He, L Zhang, J Lin and Z Zuo: Efficacy and safety of different doses of aspirin in suppressing chronic low-grade inflammation in patients with metabolic syndrome. J Pharma Pharmacol. 61:1505-10, 2009. 76. YT Lin, Z Zuo, PH Lo, SS Hseu, WK Chang, KH Chan, HB Yuan. Bilateral tension pneumothorax and tension pneumoperitoneum secondary to tracheal tear in a patient with relapsing polychondritis. J Chin Med Assoc. 72:488-91, 2009. 77. JA Antonakakis, P Ting, DC Scalzo, KK Figg, Z Zuo and AS Jorgenson: Ultrasound Does Not Improve the Success Rate of a Deep Peroneal Nerve Block at the Ankle. Reg Anesth Pain Med 35:217-21, 2010. 78. J Raphael, Y Gozal, J Rivo, N Navot and Z Zuo: Hyperglycemia Inhibits Isoflurane Postconditioning in the Rabbit Heart via Modulation of Phosphatidyl-3-Kinase/Akt and Endothelial Nitric Oxide Synthase Signaling. J Cardiovasc Pharm 55:348-57, 2010. 79. X Wu and Z Zuo: Statin posttreatment provides protection against simulated ischemia in bovine pulmonary arterial endothelial cells. Eur J Pharmacol 636:114-20, 2010. 80. M-S Gwak, L Li and Z Zuo: Morphine preconditioning reduces lipopolysaccharide and interferon-γ-induced mouse microglial cell injury via δ1 opioid receptor activation. Neuroscience 167:256-60, 2010. 81. C Zuo and Z Zuo: Spinal surgery under general anesthesia does not increase Alzheimer’s disease. Dementia and Geriatric Cognitive Disorders 29:233-9, 2010. 82. X Zhang, J Xiong, Y Jiao, G Wang, Z Zuo: Involvement of mitochondrial ATP-sensitive potassium channels in etomidate preconditioning-induced protection in human myeloid HL-60 cells. Environmental Toxicology and Pharmacology 29:320–322, 2010. 83. M Kang, J Ryu, JH Kim, H Na, Z Zuo and SH Do: Corticosterone decreases the activity of rat glutamate transporter type 3 expressed in Xenopus oocytes. Steriods 75:1113-8, 2010. 84. RJ McMurtrey and Z Zuo: Isoflurane preconditioning and postconditioning in rat hippocampasl neurons. Brain Research 1358:184-90, 2010. 85. SN Lee, L Li and Z Zuo: Glutamate transporter type 3 knockout mice have a decreased isoflurane requirement to induce loss of righting reflex. Neuroscience 171:788-93, 2010. 86. MS Gwak, L Cao, L li and Z Zuo: Isoflurane preconditioning reduces oxygen-glucose deprivation-induced neuronal injury via B-cell lymphoma 2 protein. Environmental Toxicology and Pharmacology 31:262-5, 2011. 87. D Lin, C Feng, M Cao and Z Zuo: Isoflurane may not induce significant toxicity to human neuron-like cells. Anesth Analg 112:1194-8, 2011. 88. J Feng and Z Zuo: Isoflurane preconditioning increases endothelial cell tolerance to in vitro simulated ischemia. J Pharma Pharmacol. 63:106-10, 2011. 89. L Li and Z Zuo: Glutamate transporter type 3 knockout reduces brain tolerance to focal brain ischemia in mice. J Cereb Met Blood Flow 31:1283-92, 2011. 90. Y Huang, L Li, JM Washington, X Xu, JJ Sando, D Lin and Z Zuo: Inhibition of isoflurane-

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induced Increase of cell-surface redistribution and activity of glutamate transporter type 3 by serine 465 sequence-specific peptides. Eur J Pharmacol 655:16-22, 2011. 91. C Feng, J Li and Z Zuo: Monoallelic expression of regulatory factor X1 in mouse brain. Folia Histocehmica et Cytobiogia 49:344-51, 2011 92. N Varhabhatla and Z Zuo: Patients with chronic pulmonary diseases stayed longer in hospital after neurosurgery. J Neurosurgery 115:375-379, 2011. 93. X Gao, M Wang, X He, L Tang, J Liang, L Peng, H Lin, H Sun, H Ma, J Lin and Z Zuo: Decreased Intralymphocytic Magnesium Content Is Associated with Diastolic Heart Dysfunction in Patients with Essential Hypertension. International Journal of Cardiology 147:331-4, 2011. 94. D Lin, G Li and Z Zuo: Volatile anesthetic post-treatment induces protection via inhibition of glycogen synthase kinase 3β in human neuron-like cells. Neuroscience 179:73-9, 2011. 95. C Feng, L Cao and Z Zuo: RNA interference-produced autoregulation of inducible nitric oxide synthase expression. FEBS Lett 585:2488-92, 2011. 96. D Lin and Z Zuo: Isoflurane induces hippocampal cell injury and cognitive impairments in adult rats. Neuropharmacology 61:1354-9, 2011. 97. L Li and Z Zuo: Isoflurane postconditioning induces neuroprotection via Akt activation and reduced mitochondrial membrane permeation. Neuroscience 199:44-50, 2011. 98. J Li, W Sheng, C Feng and Z Zuo: Pyrrolidine dithiocarbamate attenuates brain Aβ increase and long-term cognitive and neurological impairment in rats after focal brain ischemia. Neurobiology of Disease 45:564-72, 2012. 99. H-J Jeong, D Lin and Z Zuo: Delayed treatment with lidocaine reduces mouse microglial cell injury and cytokine production after stimulation with lipopolysaccharide and interferon γ. Anesthesia and Analgesia 114:856-61, 2012. 100. S-N Lee, S-H Park, and Z Zuo: Effects of isoflurane on learning and memory functions of wild type and glutamate transporter type 3 knockout mice. J Pharma Pharmacol. 64:302- 7, 2012. 101. NC Varhabhatla and Z Zuo: Rising complication rates after intrathecal catheter and pump placement in the pediatric population – Analysis of national data between 1997 and 2006. Pain Physician 15:65-74, 2012. 102. HS Na, HP Park, CS Kim, SH Do, Z Zuo, and CS Kim. 17β-Estradiol attenuates the activity of the glutamate transporter type 3 expressed in Xenopus oocytes. Eur J Pharmacol 676:20-5, 2012 103. D Lin, L Cao, Z Wang, J Li, JM Washington and Z Zuo: Lidocaine attenuates cognitive impairment after isoflurane anesthesia in old rats. Behav Brain Res. 228:319-27, 2012. 104. J Li , J Deng, W Sheng and Z Zuo: Metformin attenuates Alzheimer's disease-like neuropathology in obese, leptin-resistant mice. Pharmacol Biochem Behav. 101(4):564- 105. Z Zuo: Are volatile anesthetics neuroprotective or neurotoxic? Med Gas Res. 2(1):10, 2012. 106. C Feng and Z Zuo. Regulatory factor X1-induced down-regulation of transforming growth factor beta 2 transcription in human neuroblastoma cells. J Biol Chem. 287(27):22730-9, 2012. 107. Cao L, Feng C, Li L and Zuo Z: Contribution of microRNA-203 to the isoflurane preconditioning-induced neuroprotection. Brain Res Bull. 88(5):525-8, 2012. 108. L Cao, L Li and Z Zuo: N-Acetylcysteine reverses existing cognitive impairment and increased oxidative stress in glutamate transporter type 3 deficient mice. Neuroscience 220:85-9, 2012.

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109. Zhao Y, Chen X, Ma L, Zuo Z, Zhu Z, Zhu X, Wang Q, He E, Xiong L, Pei J, Xu L, Hou L, Chen S. Electroacupuncture pretreatment induces tolerance against focal cerebral ischemia through activation of canonical Notch pathway. BMC Neuroscience 13:111, 2012. 110. Z Ye, YM Huang, E Wang, Z Zuo and QL Guo: Sevoflurane-induced delayed neuroprotection involves mitoKATP channel opening and PKCε activation. Mol Biol Rep 39:5049-57, 2012. 111. Lin Cao, Liaoliao Li, Daowei Lin, and Z Zuo. Isoflurane induces learning impairment that is mediated by interleukin 1β in rodents. Plos One 7:e51431, 2012. 112. K Zhang, J Wang, H Zhang, J Chen, Z Zuo, J Wang, H Huang. Mechanisms of epoxyeicosatrienoic acids to improve cardiac remodeling in chronic renal failure disease. Eur J Pharmacol. 701(1-3):33-9, 2013. 113. JS Choi, JH Ryu, Z Zuo, SM Yang, HW Chang and SH Do: Riluzole attenuates excitatory amino acid transporter type 3 activity in Xenopus oocytes via protein kinase C inhibition. Eur J Pharmacol 713(1-3):39-43, 2013. 114. X Ren and Z Zuo: Are we ready to apply preconditioning-induced neuroprotection to improve the outcome of patients with brain ischemia? Translation Medicine Research, In press. 115. J Deng, J Li, L Li, C Feng, L Xiong and Z Zuo: Glutamate transporter type 3 knockout leads to decreased heart rate possibly via parasympathetic mechanism. Transgenic Research 22:757-66, 2013. 116. H Li, J Yin, L Li, J Deng, C Feng and Z Zuo: Isoflurane postconditioning reduces ischemia-induced nuclear factor-κB activation and interleukin 1β production to provide neuroprotection in rats and mice. Neurobiology of Disease 54:216-24, 2013. 117. I Son, HJ Shin, JH Ryu, HK Kim, SH Do and Z Zuo: Progesterone increases the activity of glutamate transporter type 3 expressed in Xenopus oocytes. Eur J Pharmacol 715: 414-9, 2013. 118. L Li, Z Wang and Z Zuo: Chronic intermittent fasting improves cognitive functions and brain structures in mice. Plos One 8(6):e66069. 119. H Huang, G Peng, M Lin, K Zhang, Y Wang, Y Yang, Z Zuo, R Chen and J Wang: The Diagnostic Threshold of HbA1c and Impact of its Use on Diabetes Prevalence--A Population-Based Survey of 6,898 Han Participants from Southern China. Preventive Medicine 57:345-50, 2013. 120. L Li, J Deng and Z Zuo: Glutamate transporter type 3 mediates isoflurane preconditioning- induced acute phase of neuroprotection in mice. Brain Research Bulletin 98:23-29, 2013. 121. X Zhu, J Yin, L Li, L Ma, H Tan, J Deng, S Chen, and Z Zuo: Electroacupuncture preconditioning-induced neuroprotection may be mediated by glutamate transporter type 2. Neurochemistry International 63:302-8, 2013. 122. F Blum and Z Zuo: Volatile anesthetics-induced neuroinflammatory and anti-inflammatory responses. Medical Gas Research 3(1):16, 2013. 123. Z Wang, H Zhao, S Peng and Z Zuo: Intranasal pyrrolidine dithiocarbamate decreases brain inflammatory mediators and provides neuroprotection after brain hypoxia-ischemia in neonatal rats. Exp Neurol 249:74-82, 2013. 124. Q Hou, Z Zuo, P Michel, Y Zhang, A Eskandari, F Mang, Q Gao, K Johnston, M

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Wintermark: Influence of chronic hyperglycemia on cerebral micro-vascular remodeling: an in-vivo study using perfusion-CT in acute ischemic stroke patients. Stroke 44:3557-60, 2013. 125. HJ Yoon, YJ Lim, Z Zuo, W Hur and SH Do: Nicotine decreases the activity of glutamate transporter type 3. Toxicol Lett. 225(1):147-52, 2014. 126. J Zhang, W Jiang and Z Zuo: Pyrrolidine dithiocarbamate attenuates surgery-induced neuroinflammation and cognitive dysfunction possibly via inhibition of nuclear factor κB. Neuroscience 261:1-10, 2014. 127. J Cao, H Tan, W Mi and Z Zuo: Glutamate transporter type 3 regulates mouse hippocampal GluR1 trafficking. Biochmi Biophys Acta 1840:1640-5, 2014. 128. C Feng, Y Zhang, J Yin, J Li, R Abounader and Z Zuo: Regulatory factor X1 is a new tumor suppressive transcription factor that acts via direct down-regulation of CD44 in glioblastoma. Neuro-Oncology 16:1078-85, 2014 (PMID: 24526308). 128. J Yin, H Li, C Feng and Z Zuo: Inhibition of brain ischemia-caused Notch activation in microglia may contribute to isoflurane postconditioning-induced neuroprotection in male rats. CNS & Neurological Disorders - Drug Targets 13:718-32, 2014. 129. J Yun, J Li and Z Zuo: Transferred inter-cell ischemic preconditioning-induced neuroprotection may be mediated by adenosine A1 receptors. Brain Research Bulletin 103:66-71, 2014. 130. HT Lin, SC Wang, Z Zuo, MY Tsou, KH Chan and HB Yuan: Increased requirement for minute ventilation and negative arterial to end-tidal carbon dioxide gradient may indicate malignant hyperthermia. J Chin Med Assoc. 77:209-12, 2014. 131. X Ren, Z Wang H Ma and Z Zuo: Sevoflurane postconditioning provides neuroprotection against brain hypoxia-ischemia in neonatal rats. Neurological Science 35:1401-4, 2014. 132. Z Wang, W Huang and Z Zuo: Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat. J Neuroinflammation 11:56 doi: 10.1186/1742-2094-11-56, 2014. 133. W Hur, MK Lee, HP Par, CS, Kim, HJ Yoon, Z Zuo and SH Do: Ondansetron attenuates the activity of excitatory amino acid transporter type 3 expressed in Xenopus oocytes. Eur J Pharmacol. 733:7-12, 2014. 134. Y Li, M Zeng, W Chen, C Liu, F Wang, X Han, Z Zuo and S Peng: Dexmedetomidine Reduces Isoflurane-Induced Neuroapoptosis Partly by Preserving PI3K/Akt Pathway in the Hippocampus of Neonatal Rats. Plos One 9:e93639, 2014. 135. SH Do, SJ Par, HJ Shin, HS Paik, Z Zuo, HJ Yoon, and JH Ryu: Dexmedetomidine increases the activity of excitatory amino acid transporter type 3 expressed in Xenopus oocytes: The involvement of protein kinase C and phosphatidylinositol 3-kinase. Eur J. Pharmacol. 738:8-13, 2014. 136. S Chiao and Z Zuo: A double-edged sword: volatile anesthetic effects on the neonatal brain. Brain Sci. 4:273-94,2014. 137. Z Wang, SH Park, H Zhao, S Peng and Z Zuo: A critical role of glutamate transporter type 3 in the learning and memory of mice. Neurobiol Learn Mem. 114C:70-80, 2014. 138. J Cao, Z wang, W Mi and Z Zuo: Isoflurane unveils a critical role of glutamate transporter type 3 in regulating hippocampal GluR1 trafficking and context-related learning and memory in mice. Neuroscience 272C:58-64, 2014. 139. H Tan, J Cao, J Zhang and Z Zuo: Critical role of inflammatory cytokines in impairing biochemical processes for learning and memory after surgery in rats. J. Neuroinflammation 11:93. doi: 10.1186/1742-2094-11-93.

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140. Z Liao, D Cao, X Han, C Liu, J Peng, Z Zuo, F Wang, and Y Li: Both JNK and p38 MAPK pathways participate in the protection by dexmedetomidine against isoflurane-induced neuroapoptosis in the hippocampus of neonatal rats. Brain Res. Bull. 107C:69-78, 2014. 141. B Naik, EL Keeley, DR Gress and Z Zuo: Case scenario: A patient on dual antiplatelet therapy with an intracranial hemorrhage after percutaneous coronary intervention. Anesthesiology 121:644-53, 2014. 142: J Zhang, H Tan, W Jiang and Z Zuo: Amantadine alleviates postoperative cognitive dysfunction possibly by increasing glial cell line-derived neurotrophic factor in rats. Anesthesiology 121:773-85, 2014. 143: J Deng, J Zhang, C Feng L Xiong and Z Zuo: Critical role of matrix metalloprotease-9 in chronic high fat diet-induced cerebral vascular remodeling and increase of ischemic brain injury in mice. Cardiovascular Res. 103:473-84, 2014. 144. Z Zhou, G Zhang, X Li, X Liu, N Wang, L Qiu, W, Liu, Z Zuo and J Yang: Loss of phenotype of parvalbumin interneurons in rat prefrontal cortex is involved in antidepressant- and propsychotic-like behaviors following acute and repeated ketamine administration. Mol Neurobiol 51:808-19, 2015. 145. H Yu, J Deng, and Z Zuo: High fat diet reduces neuroprotection of isoflurane post- treatment: role of carboxyl-terminal modulator protein-Akt signaling. Obesity 22:2396- 405, 2014. 146. P Zhao, G Ji, H Xue, W Yu, X Zhao, M Ding, Y Yang and Z Zuo: Isoflurane postconditioning improved long-term neurological outcome possibly via inhibiting the mitochondrial permeability transition pore in neonatal rats after brain hypoxia-ischemia. Neuroscience 280C:193-203, 2014 147. Y Zhang, Y Wang, Z Zuo, Z Wang, J Roy, Q Hou, E Tong, A Hoffmann, E Sperberg, J Bredno, SS Berr, M Xie, LK Lee, M Wintermark: Effects of tissue plasminogen activator timing on blood-brain barrier permeability and hemorrhagic transformation in rats with transient ischemic stroke. J Neurol Sci 347:148-54, 2014. 148. J Deng, L Xiong and Z Zuo: Trans-sodium crocetinate provides neuroprotction against cerebral ischemia and reperfusion in obese mice. J Neurosci Res. 93:615-22, 2015. 149. D Fan, J Li, B Zheng, L Hua and Z Zuo: Enriched environment attenuates surgery-induced impairment of learning, memory and neurogenesis possibly by preserving BDNF expression. Molecular Neurobiology 53:344-54, 2016. 150. K Zhang, F Huang, J Chen, Q Cai, T Wang, R Zou, Z Zuo, J Wang and H Huang: Independent influence of overweight and obesity on the regress of left ventricular hypertrophy in hypertensive patients: A meta-analysis. Medicine 93:e130, 2014. 151. Z Wang, C Feng, H Zhao, X Ren, S Peng and Z Zuo: Autoregulation of inducible nitric oxide synthase expression by RNA interference provides neuroprotection in neonatal rats. Theranostics 15:504-14, 2015. 152. Y Zhang, J Aubry, J Zhang, Y Wang, J Roy, JF Mata, W Miller, E Dumont, M Xie, K Lee, Z Zuo, M Wintermark: Defining the Optimal Age for Focal Lesioning in a Rat Model of Transcranial HIFU. Ultrasound Med. Biol. 41:449-55, 2015. 153. J Zhang, H Tan, W Jiang, Z Zuo: The choice of general anesthetics may not affect neuroinflammation and impairment of learning and memory after surgery in elderly rats. Journal of Neuroimmune Pharmacology. 10:179-89, 2015. 154. H Tan, J Bi, Y Wang, J Zhang and Z Zuo: Transfusion of old red blood cells induces neuroinflammation and cognitive impairment. Critical Care Medicine 43:e276-86, 2015. 155. J Raphael, Y Gozal, N Navot, Z Zuo: Activation of andeosine triphosphate-regulated

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potassium channels during reperfusion restores isoflurane postconditioning-induced cardiac protection in acutely hyperglycemic rabbits. Anesthesiology 122:1299-311, 2015. 156. SJ Park, HJ Shin, BW Gu, KI Woo, Z Zuo, SH Do, JH Ryu: Desflurane increased the activity of excitatory amino-acid carrier 1 (EAAC1) expressed in Xenopus oocytes. Eur J Pharmacol 757:84-9, 2015. 157. Z Wang, L Li, H Zhao, S Peng, Z Zuo: Chronic high fat diet induces cardiac hypertrophy and fibrosis in mice. Metabolism 64:917-25, 2015. 158. YS Gil, JH Kim, CH Kim, JI Han, Z Zuo, HJ Baik: Gabapentin inhibits the activity of the rat excitatory glutamate transporter 3 expressed in Xenopus oocytes. Eur J Pharmacol 762:112-117, 2015. 159. L Zhou, Z Wang, H Zhou, T Liu, F Lu, S Wang, J Li, S Peng, Z Zuo: Neonatal exposure to sevoflurane may not cause learning and memory deficits and behavioral abnormality in the childhood of Cynomolgus monkeys. Sci Rep 5:11145, 2015. 160. BI Naik, TN Pajewski, DI Bogdonoff, Z Zuo, P Clark, AS Terkawi, ME Durieux, CI Shaffrey, EC Nemergut: Rotational thromboelastometry-guided blood product management in major spine surgery. J Neurosurg Spine 22:1-11, 2015. 161. HJ Park, HJ Baik, DY Kim, GY Lee, JH Woo, Z Zuo, RK Chung: Doxepin and imipramine but not fluoxetine reduce the activity of the rat glutamate transporter EAAT3 expressed in Xenopus oocytes. BMC Anesthesiology 15:116, 2015. 162. RH Thiele, C Hucklenbruch, JZ Ma, D Colquhoun, Z Zuo, EC Nemergut, J Raphael: Admission hyperglycemia is associated with poor outcome after emergent coronary bypass grafting surgery. J Crit Care 30:1210-6, 2015. 163. F Chen, Z Long. J Yin, Z Zuo, H Li: Isoflurane post-treatment improves outcome after an embolic stroke in rabbits. Plos One 10:e0143931, 201. 164. Y Wang, R Han, and Z Zuo: Remote Ischemic Postconditioning May Provide Limited Protection in Rats with Subarachnoid Hemorrhage. J Anesth Perioper Med 2: 231-5, 2015. 165. SS Chiao, Z Zuo: Approach to Risk Management of Perioperative Stroke. J Anesth Perioper Med 2: 268-76, 2015 166. Y Wang, R Han, and Z Zuo: Dexmedetomidine post-treatment induces neuroprotection via activation of ERK in rats with subarachnoid haemorrhage. Br J Anaesth 116:384-92, 2016. 167. X Ren, H Ma and Z Zuo: Dexmedetomidine postconditioning reduces brain injury after brain hypoxia-ischemia in neonatal rats. Journal of Neuroimmune Pharmacology 11:238- 47, 2016. 167. Y Zhang , H Tan, EH Bertram, JF Aubry, MB Lopes, J Roy, E Dumont, M Xie, Z Zuo, AL Klibanov, KS Lee and M Wintermark: Non-Invasive, Focal Disconnection of Brain Circuitry Using Magnetic Resonance-Guided Low-Intensity Focused Ultrasound to Deliver a Neurotoxin. Ultrasound Med Biol. 42:2261-9, 2016. 168. JH Ryu, Z Wang, D Fan, SH Han, SH Do and Z Zuo: Isoflurane attenuates mouse microglial engulfment induced by lipopolysaccharide and interferon-γ possibly by inhibition of p38 mitogen-activated protein kinase. NeuroReport 27:1101-5, 2016. 169. J Chen, Z Gu, M Wu, Y Yang, J Zhang, J Ou, Z Zuo, J Wang and Y Chen: C-reactive protein can upregulate VEGF expression to promote ADSC-induced angiogenesis by activating HIF-1α via CD64/PI3k/Akt and MAPK/ERK signaling pathways. Stem Cell Res Ther. 16;7:114, 2016. 170. F Luo, Y Hu, W Zhao, Z Zuo, Q Yu, Z Liu, J Lin, Y Feng, B Li, L Wu and L Xu: Maternal

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Exposure of Rats to Isoflurane during Late Pregnancy Impairs Spatial Learning and Memory in the Offspring by Up-Regulating the Expression of Histone Deacetylase 2. PLoS One 11:e0160826, 2016. 171. K Pan, X Li, Y Chen, D Zhu, Y Li, G Tao and Z Zuo: Deferoxamine pre-treatment protects against postoperative cognitive dysfunction of aged rats by depressing microglial activation via ameliorating iron accumulation in hippocampus. Neuropharmacology 111:180-94, 2016. 172. Y Lu, S Lei, N Wang, P Lu, W Li, J Zheng, PK Giri, H Lu, X Chen, Z Zuo, Y Liu and P Zhang: Protective effect of minocycline against ketamine-induced injury in neural stem cell: involvement of PI3K/Akt and GSK-2 beta pathway. Frontiers in Molecular Neuroscience 9:135, 2016 173. RH Thiele, K Ikeda, Y Wang, Y Nartz and Z Zuo: Broadband near-infrared spectroscopy can detect cyanide-induced cytochrome aa3 inhibition in tats: a proof of concept study. Canadian Journal of Anaesthesia 64:376-384, 2017 174. J Luo, X Chen, C Luo, G Lu, L Peng, X Gao and Z Zuo: Hydrochlorothiazide modulates ischemic heart failure-induced cardiac remodeling via inhibiting angiotensin II type 1 receptor pathway in rats. Cardiovascular Therapeutics 35:e12246, 2017 175. L Wan, J Bi, J Li and Z Zuo: Glutamate transporter type 3 participates in maintaining morphine-induced conditioned place preference. Neuroscience 344:67-73, 2017 176. B Zheng, R Lai, J Li and Z Zuo: Critical role of P2X7 receptors in the neuroinflammation and cognitive dysfunction after surgery. Brain, Behavior, and Immunity 61:365-374, 2017 177. J Bi, W Shan, A Luo and Z Zuo: Critical role of matrix metallopeptidase 9 in postoperative cognitive dysfunction and age-dependent cognitive decline. Oncotarget in press. doi: 10.18632/oncotarget.15545 178. R Cao, J Li, B Ning, N Sun, T Wang, Z Zuo and S Hu: Functional and oxygen-metabolic photoacoustic microscopy of the awake mouse brain. Neuroimage 150:77-87, 2017 179. L Gui, X Lei and Z Zuo: Decrease of glial cell-derived neurotrophic factor contributes to anesthesia and surgery-induced learning and memory dysfunction in neonatal rats. Journal of Molecular Medicine 95:369-379, 2017 180.Y Wang, R Han and Z Zuo: Dexmedetomidine-induced neuroprotection: is it translational? Transl Perioper Pain Med 1:15-19, 2016 181.Y Lu, PK Gir, S Lei, J Zheng, W Li, N Wang, X Chen, H Lu, Z Zuo, Y Liu and P Zhang: Pretreatment with minocycline restores neurogenesis in the subventricular zone and subgranular zone of the hippocampus after ketamine exposure in neonatal rats. Neuroscience 352:144-154, 2017. 182.C Feng, BI Naik, W Xin, JZ Ma, DC Scalzo, S Thammishetti, RH Thiele, Z Zuo, and J Raphael: Haptoglobin 2-2 Phenotype is Associated with Increased Acute Kidney Injury after Elective Cardiac Surgery in Patients with Diabetes. JAHA: Journal of the American Heart Association 6:e006565, 2017. 183.Z Wang, W Shan, J Cao, M Wintermark, W Huang, and Z Zuo: Early administration of pyrrolidine dithiocarbamate extends the therapeutic time window of tissue plasminogen activator in a male rat model of embolic stroke. Journal of Neuroscience Research 96:449- 458, 2018. 184.F Chen, G Duan, Z Wu, Z Zuo, H Li: Comparison of the cerebroprotective effect of inhalation anaesthesia and total intravenous anaesthesia in patients undergoing cardiac

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surgery with cardiopulmonary bypass: a systematic review and meta-analysis. BMJ Open 7:e014629 185.H Zhao, Y Yang, Y Lu, M Mort, DN Cooper, Z Zuo, Y Zhou: Quantitative mapping of genetic similarity in human heritable diseases by shared mutations. Hum Mutat. 39:292-301, 2018 186.J Li, W Shan, Z Zuo: Age-Related Upregulation of Carboxyl Terminal Modulator Protein Contributes to the Decreased Brain Ischemic Tolerance in Older Rats. Mol. Neurobiol. 55:6145-6154, 2018 187.X Han, C Liu, K Zhang, M Guo, Z Shen, Y Liu, Z Zuo, M Cao, Y Li: Calpain and JNK pathways participate in isoflurane - induced nucleus translocation of apoptosis-inducing factor in the brain of neonatal rats. Toxicol Lett. 2017 Dec 28;285:60-73 188.M Good, S Eucker, J Li, H Bacon, S Lang, J Butcher, T Johnson, R Gaykema, M Patel, Z Zuo, B Isakson: Endothelial cell Pannexin1 modulates severity of ischemic stroke by regulating cerebral inflammation and myogenic tone. Journal of Clinical Investigation Insight 3:pii: 96272, 2018 189.W Xing, P Huang, Y Lu, W Zeng, Z Zuo: Amantadine attenuates sepsis-induced cognitive dysfunction possibly not through inhibiting toll-like receptor 2. Journal of Molecular Medicine 96:391-402, 2018 190.JH Ryu, SH Do, SH Han, Z Zuo: Morphine reduces mouse microglial engulfment induced by lipopolysaccharide and interferon-γ via δ opioid receptor and p38 mitogen-activated protein kinase. Neurol Res. 27:1-7, 2018 191.Z Wang, S Meng, L Cao, Y Chen, Z Zuo, S Peng: Critical role of NLRP3-caspase-1 pathway in age-dependent isoflurane-induced microglial inflammatory response and cognitive impairment. J Neuroinflammation 15:109, 2018 192.Y Xu, X Rong, W Hu, X Huang, Y Li, D Zheng, Z Cai, Z Zuo, Y Tang: Bevacizumab Monotherapy Reduces Radiation-induced Brain Necrosis in Nasopharyngeal Carcinoma Patients: A Randomized Controlled Trial. Int J Radiat Oncol Biol Phys. 101(5):1087-1095, 2018 193.RH Thiele, K Ikeda, HP Osuru, Z Zuo: Comparison of Broadband and Discrete Wavelength Near-Infrared Spectroscopy Algorithms for the Detection of Cytochrome aa3 Reduction. Anesth Analg. In press 194.P Liang, W Shan, Z Zuo: Perioperative use of cefazolin ameliorates postoperative cognitive dysfunction but induces gut inflammation in mice. J Neuroinflammation 15:235, 2018 195.M Berger, KJ Schenning, CH Brown 4th, SG Deiner, RA Whittington, RG Eckenhoff, MS Angst, S Avramescu, A Bekker, M Brzezinski, G Crosby, DJ Culley, M Eckenhoff, LI Eriksson, L Evered, J Ibinson, RP Kline, A Kofke, D Ma, JP Mathew, M Maze, BA Orser, CC Price, DA Scott, B Silbert, D Su, N Terrando, DS Wang, H Wei, Z Xie, Z Zuo: Perioperative Neurotoxicity Working Group. Best Practices for Postoperative Brain Health: Recommendations From the Fifth International Perioperative Neurotoxicity Working Group. Anesth Analg. 127(6):1406-1413, 2018. 196. R Cao, J Li, Y Kharel, C Zhang, K Lynch*, Z Zuo*, Song Hu*: Photoacoustic microscopy reveals the role of sphingosine-1-phosphate induced neuroprotection against ischemic stroke. Theranostics 8(22):6111-6120, 2018. *co-corresponding authors. 197. R Cao, C Zhang, V Mitkin, M Lankford, J Li, Z Zuo, C Goyne, C Meyer, J Stone, S Hu: Comprehensive Characterization of Cerebrovascular Dysfunction in Blast Traumatic Brain Injury Using Photoacoustic Microscopy. Journal of Neurotrauma In press.

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198. R Cao, J Li, C Zhang, Z Zuo*, S Hu*: Photoacoustic Microscopy of Obesity-induced Cerebrovascular Alternations. Neuroimage 188:3690379, 2018. *co-corresponding authors. 199.N Lunardi, R Sica, N Atluri, KA Salvati, C Keller, M Beenhakker, H Goodkin, Z Zuo: Disruption of REM sleep homeostasis in adolescent rats after neonatal anesthesia. Anesthesiology In press. 200.G Duan, X Bao, G Yang, J Peng, Z Wu, P Zhao, Z Zuo, H Li: Patient-controlled intravenous tramadol versus patient-controlled intravenous hydromorphone for analgesia after secondary cesarean delivery: a randomized controlled trial to compare analgesic, anti- anxiety and anti-depression effects. J Pain Res 18;12:49-59, 2018. 201.W Shan, J Li, W Xu, H Li, Z Zuo: Critical role of UQCRC1 in embryo survival, brain ischemic tolerance and normal cognition in mice. Cellular and Molecular Life Sciences in press 202.R Lai, W Shan, D Zhou, X Zeng, K Zuo, D Pan, W Zeng, Z Zuo: Sevoflurane enhances cancer behavior of human glioblastoma cells possibly via increasing the expression of clusters of differentiation 44. Acta Pharmacologica Sinica in press

Published editorial 1. Z Zuo and LS Sorkin: Molecular mechanism of cross-organ reflex sensitization: Should I pay attention to it? Anesthesiology 114:14-5, 2011. 2. Z Zuo: A novel mechanism for sevoflurane preconditioning-induced neuroprotection. Anesthesiology 117:942-4, 2013. 3. Z Zuo: Postoperative cognitive effects in newborns: the role of inflammatory processes. Anesthesiology 118:481-3, 2013.

Published correspondence 1. Z Zuo and H-B Yuan: Hypothermic preconditioning-induced neuroprotection. Anesthesiology 101:1043-4, 2004. 2. WJ Mauermann, AM Shilling and Z Zuo: Prior Work Using Meta-Analyses of Operative Times between Regional and General Anesthesia. Anesthesia &Analgesia 104:459, 2007 3. X Xu, S She, S Yao, M Mok, Z Zuo: Respiratory inhibition and difficult ventilation after inadvertent epidural administration of remifentanil. Anesthesia &Analgesia 104:1004, 2007 4. Z Zuo, Z Wang, S Meng, S Peng: A response to readers’ comments. J Neuroinflammation 15:267, 2018

Publications in Peer-reviewed English Journals as a group of authors (resulted from participating in a major clinical study) 1. Anderson SW, Todd MM, Hindman BJ, Clarke WR, Torner JC, Tranel D, Yoo B, Weeks J, Manzel KW, Samra S; IHAST Investigators. Effects of intraoperative hypothermia on neuropsychological outcomes after intracranial aneurysm surgery. Ann Neurol. 60(5):518-27, 2006. 2. SK Samra, B Giordani, AF Caveney, WR Clarke, PA Scott, S Anderson, BG Thompson, MM Todd, for the CFAAST Investigators. Recovery of Cognitive Function After Surgery for Aneurysmal Subarachnoid Hemorrhage. Stroke 38:1864-1872, 2007 3. Leira EC, Davis PH, Martin CO, Torner JC, Yoo B, Weeks JB, Hindman BJ, Todd MM;

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Intraoperative Hypothermia for Aneurysm Surgery Trial (IHAST) Investigators. Improving prediction of outcome in "good grade" subarachnoid hemorrhage Neurosurgery. 61(3):470-3, 2007. 4. McGregor DG, Lanier WL, Pasternak JJ, Rusy DA, Hogan K, Samra S, Hindman B, Todd MM, Schroeder DR, Bayman EO, Clarke W, Torner J, Weeks J; Intraoperative Hypothermia for Aneurysm Surgery Trial Investigators. Effect of nitrous oxide on neurologic and neuropsychological function after intracranial aneurysm surgery. Anesthesiology. 108(4):568-79, 2008. 5. Pasternak JJ, McGregor DG, Schroeder DR, Lanier WL, Shi Q, Hindman BJ, Clarke WR, Torner JC, Weeks JB, Todd MM; IHAST Investigators. Hyperglycemia in patients undergoing cerebral aneurysm surgery: its association with long-term gross neurologic and neuropsychological function. Mayo Clin Proc. 83(4):406-17, 2008. 6. Coghlan LA, Hindman BJ, Bayman EO, Banki NM, Gelb AW, Todd MM, Zaroff JG; IHAST Investigators. Independent associations between electrocardiographic abnormalities and outcomes in patients with aneurysmal subarachnoid hemorrhage: findings from the intraoperative hypothermia aneurysm surgery trial. Stroke. 40(2):412-8, 2009 7. Pasternak JJ, McGregor DG, Lanier WL, Schroeder DR, Rusy DA, Hindman B, Clarke W, Torner J, Todd MM; IHAST Investigators. Effect of nitrous oxide use on long-term neurologic and neuropsychological outcome in patients who received temporary proximal artery occlusion during cerebral aneurysm clipping surgery. Anesthesiology 110(3):563-73, 2009. 8. Todd MM, Hindman BJ, Clarke WR, Torner JC, Weeks JB, Bayman EO, Shi Q, Spofford CM; IHAST Investigators. Perioperative fever and outcome in surgical patients with aneurysmal subarachnoid hemorrhage. Neurosurgery 64(5):897-908, 2009. 9. Hindman BJ, Bayman EO, Pfisterer WK, Torner JC, Todd MM; IHAST Investigators. No association between intraoperative hypothermia or supplemental protective drug and neurologic outcomes in patients undergoing temporary clipping during cerebral aneurysm surgery: findings from the Intraoperative Hypothermia for Aneurysm Surgery Trial. Anesthesiology 112(1):86-101, 2010. 10. Nguyen HP, Zaroff JG, Bayman EO, Gelb AW, Todd MM, Hindman BJ; IHAST-MIDS and IHAST Investigators. Perioperative hypothermia (33 degrees C) does not increase the occurrence of cardiovascular events in patients undergoing cerebral aneurysm surgery: findings from the Intraoperative Hypothermia for Aneurysm Surgery Trial. Anesthesiology 113(2):327-42, 2010. 11. Mahaney KB, Todd MM, Torner JC; IHAST Investigators. Variation of patient characteristics, management, and outcome with timing of surgery for aneurysmal subarachnoid hemorrhage. J Neurosurg 114:1045-53, 2011. 12. Mahaney KB, Todd MM, Bayman EO, Torner JC; for the IHAST Investigators. Acute postoperative neurological deterioration associated with surgery for ruptured intracranial aneurysm: incidence, predictors, and outcomes. J Neurosurg. 116(6):1267-1278, 2012. 13. EO Bayman, KM Chaloner, BJ Hindman, MM Todd and IHAST Investigators: Bayesian methods to determine performance differences and to quantify variability among centers in multi-center trials: the IHAST trial. BMC Med Res Methodol. 13:5 (doi: 10.1186/1471- 2288-13-5), 2013

Manuscript in revision

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About 7 manuscripts with Z Zuo as the corresponding authors are currently in revision for various journals such as Cardiovascular Research and Anesthesiology. About half are minor revision and the others are major revision.

Manuscripts Submitted/in Review Four manuscripts are under initial review.

Selected Publications in Chinese Journals 1. Z Zuo and C Gao: Atracurium: a new neuromuscular blocker. Guangdone Medical J. 9:42-47, 1988. 2. Z Zuo and C Gao: Atracurium for endotracheal intubation. Guangdone Medical J. 10:47-49, 1989. 3. Z Zuo and C Gao: Continuous observation of the dynamic changes of intracranial pressure and cerebral blood perfusion during the process of hypotension induced by sodium nitroprusside. Chinese J. Anesthesiology 9:332-337, 1989. 4. Z Zuo and C Gao: The effects of endotracheal intubation on the state of brain blood perfusion. J. Clinical Anesthesiology 5:102-111, 1990. 5. Z Zuo and C Gao: The anesthetic management of hepatectomy under the conditions of normal body temperature and temporary occlusion of the afferent hepatic circulation. J. Clinical Anesthesiology 5:112-119, 1990.

Books and Book Chapters 1. RA Johns, Z Zuo, Rengasamy A, Pajewski T and E Haddad: Effects of anesthetics on NO synthase activity and signaling. Published Symposia of the 25th Anniversary of the clinic of Anesthesiology, Intensive Care Medicine and Pain Control. Frankfurt, Germany. June, 1998. 2. DE Traul, Z Zuo and TN Pajewski: Anesthetic induced neuroprotection: a review. In Advance in Anesthesia, 26 (2008):201-12. 3. Z Zuo: Anesthesia: what you need to know. A book published by PublishAmerican in 2011. 4. Z Zuo: Medical gases: volatile anesthetics, hyperbaric oxygen and hydrogen sulfide. In Innate Neuroprotection for Stroke. Ed: JM Gidday, M Perez-Pinzon, JH Zhang, Springer, 2012 5. JE Mass and Z Zuo: Delta opioid receptor in cerebral precondititioning. In Opioid Receptor Biology. Ed: Ying Xia, Springer, 2014.

Abstracts (not updated) 1. J Brown and Z Zuo: Rat kidney expresses GC-A, not GC-B receptors for natriuretic peptides. J. Physiol. Lond. 446:90p, 1992. 2. J Brown and Z Zuo: In vitro receptor binding of C-type natriuretic peptide (CNP(1-22)) in rat brain. J. Physiol. Lond. 446:440p, 1992. 3. J Brown and Z Zuo: Natriuretic peptide receptors and the growth of fetal brain. J. Physiol. Lond. 452;89p, 1992. 4. J Brown and Z Zuo: Cyclic guanosine monophosphate (cGMP) production stimulated in isolated rat glomeruli by natriuretic peptides. J. Physiol. Lond. 452:92p, 1992. 5. J Brown and Z Zuo: Cyclic 3’5’ guanosine monophosphate (cGMP) is stimulated by C- type natriuretic peptide (CNP(1-22)) in the fetal telencephalon of rats. J. Physiol. Lond. 459:198p, 1993. 6. J Brown and Z Zuo: Heterogeneity in the C-ANP binding proteins of rat renal glomeruli. J.

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Physiol. Lond. 459:363p, 1993. 7. J Brown and Z Zuo: A novel natriuretic peptide receptor in fetal rats. J. Physiol. Lond. 459:472p, 1993. 8. J Brown and Z Zuo: Low concentrations of C-type natriuretic peptide (CNP(1-22)) inhibit cyclic 3’5’ adenosine monophosphate levels in rat glomeruli. J. Physiol. Lond. 467:83p, 1993. 9. J Brown and Z Zuo: C-type natriuretic peptide (CNP(1-22)) is poorly internalized by the natriuretic peptide clearance receptor (ANPR-C). J. Physiol. Lond. 467:82p, 1993. 10. J Brown and Z Zuo: The natriuretic peptide receptor of fetal somites in the rats. J. Physiol. Lond. 479:76p, 1994. 11. J Brown and Z Zuo: Electrophoretic properties of the natruretic peptide receptors of the adult rat glomerulus. J. Physiol. Lond. 480:83p, 1994. 12. Z Zuo, A Tichotsky and RA Johns: Halothane and isoflurane do not affect inducible nitric oxide synthase activity. Anesthesiology 83(3A):A293, 1995. 13. Z Zuo and RA Johns: Localization and quantitative detection of cGMP changes caused by halothane or isoflurane in rat cerebellum. Anesthesiology 83(3A):A699, 1995. 14. Z Zuo, A Tichotsky and RA Johns: Halothane and isoflurane inhibit the NMDA induced cyclic GMP accumulation in cultured rat hippocampal neurons. The FASEB Journal 10:A926, 1996. 15. Z Zuo and RA Johns: Halothane and isoflurane upregulate the gene expression and activity level of constitutive endothelial nitric oxide synthase. Anesthesiology 85:A375, 1996 16. Z Zuo and RA Johns: Upregulation of the gene expression and activity level of inducible nitric oxide synthase by halothane and isoflurane. Anesthesiology 85:A374, 1996. 17. Z Zuo and RA. Johns: Inhalational anesthetics inhibit glutamate receptor-mediated cGMP increase. The Fifth International Conference on Molecular and Cellular Mechanisms of Anesthesia, Calgary, Canada. June, 1997. 18. Z Zuo, A Tichotsky and RA Johns: Inhibition of excitatory neurotransmission-nitric oxide signaling pathway by inhalational anesthetics: A mechanism of anesthesia? Anesthesiology 87:A631, 1997. 19. Z Zuo, A Tichotsky, T Pajewski and RA Johns: Isoflurane but not halothane enhances the inhibitory effects of alpha2-adrenoceptor-mediated cyclic GMP production in the cultured neurons of rat. Anesthesiology 89:A746, 1998. 20. Z Zuo, A Tichotsky and RA Johns: Isoflurane does not affect the protein expression of constitutive neuronal nitric oxide synthase. Anesthesiology 91:A814, 1999. 21. Z Zuo: Isoflurane increases the activity of glutamate transporters in rat glial cells. Anesthesia and Analgesia 90:S481, 2000. 22. S-H Do and Z Zuo: The effects of volatile anesthetics on glutamate transporter type 3 activity: Role of protein kinase C. The Sixth International Conference on Molecular and Basic Mechanisms of Anesthesia, Bonn, Germany. June, 2001. 23. S-H Do, J Washington, GL Kamatchi and Z Zuo: Effects of isoflurane on glutamate transporter, EAAC: Role of protein kinase C. American Society of Anesthesiologists Annual Meeting, New Orleans, LA. October, 2001. 24. Z Zuo: The effects of isoflurane and ischemic preconditioning on Purkinje neuron survival after an ischemic insult”, American Society of Anesthesiologists Annual Meeting, New Orleans, LA. October, 2001.

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25. S-H Do and Z Zuo: Effects of propofol on glutamate transporter, EAAC1: Role of protein kinase C. American Society of Anesthesiologists Annual Meeting, Orlando, Florida. October, 2002. 26. Y Huang and Z Zuo: Isoflurane, a volatile anesthetic, enhances the expression of glutamate transporter type 3. Society for Neuroscience Annual Meeting, Orlando, Florida. November, 2002. 27. Y-J Lim and Z Zuo: Morphine preconditions Purkinje cells against cell death under in vitro simulated ischemia-reperfusion conditions. American Society of Anesthesiologists Annual Meeting, San Francisco, California. October, 2003. 28. S Zheng and Z Zuo: Roles of ERK1/2 mitogen-activated protein Kinases in isoflurane preconditioning-induced neuroprotection. Society for Neuroscience Annual Meeting, New Orleans, LA. November, 2003. 29. P Zhao and Z Zuo: Isoflurane preconditioning induces inducible nitric oxide synthase- dependent neuroprotection in the neonatal rats. Association of University Anesthesiologists Annual Meeting, Sacramento, CA, 2004. 30. P Zhao and Z Zuo: Prenatal hypoxia-induced adaptation and neuroprotection that is inducible nitric oxide synthase-dependent. American Society of Anesthesiologists Annual Meeting, Las Vegas, Nevada, October, 2004. 31. EC Nemergut, KE Littlewood, Z Zuo and ER Laws: Increased incidence of postoperative diabetes insipidus in patients undergoing transsphenoidal surgery complicated by intraoperative CSF leak. American Society of Anesthesiologists Annual Meeting, Las Vegas, Nevada, October, 2004. 32. EC Nemergut, Z Zuo, KE Littlewood and ER Laws: Increased postoperative pain and vomiting in patients undergoing transsphenoidal surgery complicated by intraoperative CSF leak. American Society of Anesthesiologists Annual Meeting, Las Vegas, Nevada, October, 2004. 33. EC Nemergut, Z Zuo: Diabetes insipidus after transsphenoidal surgery. Association of University Anesthesiologists Annual Meeting, Baltimore, Maryland, May, 2005. 34. Jane JA Jr, Nemergut EC, Zuo Z, Mahoozi L, Vance ML, Laws ER: Diabetes Insipidus after Transsphenoidal Surgery: Review of 881 Patients. The Endocrine Society- ENDO 2005, San Diego, CA, June 5, 2005. 35. BJ Mauermann, AM Shilling and Z Zuo: A Comparison Of Neuraxial Versus General Anesthesia For Elective Total Hip Replacement: A Meta-Analysis. American Society of Anesthesiologists Annual Meeting, Atlanta, GA, October, 2005. 36. H Yuan, Y Huang and Z Zuo: Hypothermic Preconditioning Reduces Purkinje cell death by Preventing the Overexpression of Inducible Nitric Oxide Synthase in Rat Cerebellar Slices after an In Vitro Simulated Ischemia. American Society of Anesthesiologists Annual Meeting, Atlanta, GA, October, 2005. 37. K Ma, S Zheng and Z Zuo: Transcription factor RFX1 specifically increases the expression of neuronal glutamate transporter type 3. Society for Neuroscience Annual Meeting, Washington DC. November, 2005. 38. P Zhao, Y Huang and Z Zuo: Opioid preconditioning induces an opioid receptor-dependent delayed neuroprotection against ischemia in rats. American Society of Anesthesiologists Annual Meeting, Chicago, IL, October, 2006. 39. P Zhao, L Peng, L Li, X Xu and Z Zuo: Isoflurane preconditioning improves long-term neurological outcome after ischemia. American Society of Anesthesiologists Annual Meeting, San Francisco, CA, October, 2007.

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40. FM Gazoni and Z Zuo: The transphenoidal resection of a pituitary tumor: A medical challenging case. American Society of Anesthesiologists Annual Meeting, San Francisco, CA, October, 2007. 41. JJ Lee, L Li, HH Jung and Z Zuo: Postconditioning with isoflurane reduced brain injury in rat corticostriatal slices after an in vitro simulated ischemia. Society for Neuroscience Annual Meeting, San Diego, CA. November, 2007. 42. L Li and Z Zuo: Isoflurane preconditioning improved long-term neurological outcome in adult rats after transient focal cerebral ischemia. Society for Neuroscience Annual Meeting, Washington DC. November, 2008. 43. Z. Zuo, J. Kim: Isoflurane induces a postconditioning effect on bovine pulmonary arterial endothelial cells exposed to oxygen-glucose deprivation. International Anesthesia Research Society Annual Meeting, San Diego, CA, March, 2009. 44. Z Zuo, S-A Lee and J-G Choi: Volatile Anesthetics Attenuate Oxidative Stress-Reduced Activity of Glutamate Transporter Type 3. American Society of Anesthesiologists Annual Meeting, New Orleans, LA, October, 2009. 45. Z Ye, Y Huang, Q Guo, E Wang and Z Zuo: Sevoflurane Induced Delayed Neuroprotection Depends on PKCε and Opening mitoK ATP Channel. American Society of Anesthesiologists Annual Meeting, New Orleans, LA, October, 2009. 46. Z Zuo and L Li: Pretreatment with Isoflurane, but Not Desflurane, Improves Neurologic Outcome after Brain Ischemia. American Society of Anesthesiologists Annual Meeting, New Orleans, LA, October, 2009. 47. J Raphael, Y Gozal, N Navot and Z Zuo: Hyperglycemia Inhibits Isoflurane Postconditioning Via of PI3-Kinase/Akt and eNOS Signaling. American Society of Anesthesiologists Annual Meeting, New Orleans, LA, October, 2009. 48. J Raphael, Y Gozal, N Navot and Z Zuo: Simvastatin Enhances Pharmacological Preconditioning by Isoflurane Via PI3K/Akt Signaling. American Society of Anesthesiologists Annual Meeting, New Orleans, LA, October, 2009. 49. NC Varhabhatla and Z Zuo: Patients with Chronic Pulmonary Diseases Stayed Longer in Hospital after Neurosurgery. American Society of Anesthesiologists Annual Meeting, San Diego, CA, October, 2010. 50. RJ McMurtrey and Z Zuo: Isoflurane Preconditioning and Postconditioning in Rat Hippocampal Neurons. American Society of Anesthesiologists Annual Meeting, San Diego, CA, October, 2010. 51. D Lin, G Li and Z Zuo: Volatile Anesthetic Postconditioning in Human Neuron-Like Cell: Role of Glycogen Synthase Kinase 3. American Society of Anesthesiologists Annual Meeting, San Diego, CA, October, 2010. 52. S Sharma, S David, J Washington, Z Zuo and J Raphael: Decreased Expression of VEGF and EPO and Impaired Coronary Collaterals in Patients with Diabetes. American Society of Anesthesiologists Annual Meeting, San Diego, CA, October, 2010. 53. J Li and Z Zuo: Pyrrolidine dithiocarbamate attenuates accumulation of β-amyloid peptide in the brain and impvoed learning and memory functions in rats after focal brain ischemia. Society for Neuroscience Annual Meeting, San Diego, CA, October, 2010. 54. D Lin, J Li and Z Zuo: Lidocaine attenuates cognitive impairment after isoflurane anesthesia in old rats. International Anesthesia Research Society Annual Meeting, Vancouver, Canada, May, 2011

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55. NC Varhabhatla and Z Zuo: Rising Complication Rates After Intrathecal Catheter and Pump Placement in the Pediatric Population - Analysis of National Data. American Society of Anesthesiologists Annual Meeting, Chicago, IL, October, 2011 56. Z Zuo, H-J Jeong, D Lin: Delayed Treatment with Lidocaine Reduces Mouse Microglial Cell Injury and Cytokins Production after Stimulation With Lipopolysaccharide and Interferon γ. American Society of Anesthesiologists Annual Meeting, Chicago, IL, October, 2011 57. J Raphael, Y Gozal, Z Zuo: Activation of K¬ATP Channels Restores Anesthetic-Induced Cardiac Protection During Reperfusion in the Presence of Hyperglycemia. American Society of Anesthesiologists Annual Meeting, Chicago, IL, October, 2011 58. NC Varhabhatla and Z Zuo: Patients with Congestive Heart Failure have a Five-fold Increase in Mortality after Neurosurgery. American Society of Anesthesiologists Annual Meeting, Chicago, IL, October, 2011 59. Z Zuo and L Li: Isoflurane postconditioning induces neuroprotection via AKT activation and reduced mitochondrial membrane permeation. Society for Neuroscience Annual Meeting, Washington, DC, November, 2011.

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