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Pathogen and Product Description H. Ducreyi + C. Trachomatis (LGV)

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Pathogen and Product Description H. Ducreyi + C. Trachomatis (LGV) H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit Pathogen and product description aemophilus ducreyi is the causative agent disease (PID), ectopic pregnancy, and infertility. Ct is of chancroid, a genital ulcer disease (GUD). also responsible for outbreaks of lymphogranuloma H The organism is usually spread during sexual venereum (LGV) and trachoma, a chronic ocular intercourse through microabrasions, and the disease disease that can lead to blindness. usually manifests as multiple painful superficial ulcers associated with inguinal lymphadenitis. As a result of LGV is caused by specific serovars of Chlamydia the painful nature of the lesions, patients usually seek trachomatis (L1, L2 and L3) and these strains are immediate treatment, and asymptomatic carriage is associated with a more chronic and invasive infection therefore uncommon. In addition to causing GUD, H. than other serovars. Although LGV symptoms can ducreyi has been found in several recent studies to vary according to site of entry and stage of infection, be a major cause of chronic skin ulceration in children genital ulceration and inguinal lymphadenopathy are from developing countries. the classical presentations of this disease. Chancroid or soft chancre is a sexually transmitted VIASURE H. ducreyi + C. trachomatis (LGV) Real Time disease (STD) of humans, characterized by painful PCR Detection Kit is designed for the diagnosis of genital ulcers. The primary ulcer is often followed by Haemophilus ducreyi and/or Chlamydia trachomatis multiple lesions, which can extend the duration of the (LGV strains) in clinical samples. After DNA isolation, illness to 1-3 months if appropriate treatment is not the identification of Haemophilus ducreyi and LGV- given. associated strains of Chlamydia trachomatis is performed by the amplification of a conserved region Chlamydia trachomatis (Ct) is a gram-negative obligate of the 16S rRNA gene for Haemophilus ducreyi and intracellular pathogen responsible for over 110 million pmpH gene for LGV-associated C. trachomatis global sexually transmitted disease (STD) cases serovars, using specific primers and a fluorescent– annually, with sequelae including pelvic inflammatory labelled probe. THE REAL ONE STEP qPCR Ficha H. ducreyi + C. trachomatis-ING.indd 1 02/09/18 09:47 H. duccreyi + C. trachomatis (LGVV) Real Timme PCR Detection Kit Analytical sensitivity VIASURE H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit has a detection limit of ≥ 10 DNA copies per reaction for H. ducreyi + C. trachomatis. (figures 1 and 2). Figure 1. Dilution series of H. ducreyi (107–101 copies/rxn) template run on the Figure 2. Dilution series of C. trachomatis (LGV) (107–101 copies/rxn) template run Bio-Rad CFX96™ Real-Time PCR Detection System (channel ROX). on the Bio-Rad CFX96™ Real-Time PCR Detection System (channel FAM). Components Work Flow One-step rehydration of Reagent/Material Description Colour Quantity wells and add your extracted DNA H. ducreyi + C. trachomatis A mix of enzymes, primers-probes, White 6/12 X 8-well (LGV) 8-well strips buffer, dNTPs, stabilizers and Internal strip control in stabilized format Rehydration Buffer Solution to reconstitute the stabilized Blue 1 vial x 1,8 product mL H. ducreyi + C. trachomatis Non-infectious synthetic lyophilized Red 1 vial (LGV) Positive Control cDNA STEP 1 Negative Control Non template control Violet 1 vial x 1 mL Add 15 μl of rehydration buffer Water RNAse/DNAse free Water RNAse/DNAse free White 1 vial x 1 mL into each well Tear-off 8-cap strips Optical caps for sealing Wells during Transparent 6/12 x 8-cap thermal cycling strip Kit References STEP 2 Add 5 μl of DNA sample / Reference Description positive control / negative control VS-HCT106L Viasure H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit 6 x 8-well strips, low profile VS-HCT106H Viasure H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit 6 x 8-well strips, high profile VS-HCT112L Viasure H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit 12 x 8-well strips, low profile VS-HCT112H Viasure H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit STEP 3 12 x 8-well strips, high profile Load the strips into the thermocycler and run VS-HCT113L Viasure H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit the specified protocol 96-well plate, low profile VS-HCT113H Viasure H. ducreyi + C. trachomatis (LGV) Real Time PCR Detection Kit 96-well plate, high profile STEP 4 Interpretate results CERTEST BIOTEC, S.L. Pol. Industrial Río Gállego II, Calle J, Nº 1, 50840, San Mateo de Gállego, Zaragoza (SPAIN) www.certest.es ,i>Ê/iÊ* ,Ê iÌiVÌÊÌÃÊUÊ6iÀÃ\Ê /än£n ,i>Ê/iÊ* ,Ê iÌiVÌÊÌÃÊUÊ6iÀÃ\Ê /än£n Ficha H. ducreyi + C. trachomatis-ING.indd 2 02/09/18 09:48.
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