VPS26A Rabbit Pab

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Leader in Biomolecular Solutions for Life Science VPS26A Rabbit pAb Catalog No.: A14265 Basic Information Background Catalog No. This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded A14265 protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Observed MW The close structural similarity between the yeast and human proteins that make up this 38kDa complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of Calculated MW the retromer complex. Alternative splicing results in multiple transcript variants 28kDa/38kDa encoding different isoforms. Category Primary antibody Applications WB, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 9559 O75436 IF 1:50 - 1:200 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 208-327 of human VPS26A (NP_004887.2). Synonyms VPS26A;HB58;Hbeta58;PEP8A;VPS26 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using VPS26A antibody (A14265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. Immunofluorescence analysis of NIH-3T3 cells using VPS26A Polyclonal Antibody (A14265) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Atypical Parkinsonism–Associated Retromer Mutant Alters Endosomal Sorting of Specific Cargo Proteins
JCB: Report Atypical parkinsonism–associated retromer mutant alters endosomal sorting of specific cargo proteins Kirsty J. McMillan,1 Matthew Gallon,1* Adam P. Jellett,1* Thomas Clairfeuille,3 Frances C. Tilley,1 Ian McGough,1 Chris M. Danson,1 Kate J. Heesom,2 Kevin A. Wilkinson,1 Brett M. Collins,3 and Peter J. Cullen1 1School of Biochemistry and 2Proteomics Facility, School of Biochemistry, University of Bristol, Bristol BS8 1TD, England, UK 3Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland 4072, Australia The retromer complex acts as a scaffold for endosomal protein complexes that sort integral membrane proteins to vari- ous cellular destinations. The retromer complex is a heterotrimer of VPS29, VPS35, and VPS26. Two of these paral- ogues, VPS26A and VPS26B, are expressed in humans. Retromer dysfunction is associated with neurodegenerative disease, and recently, three VPS26A mutations (p.K93E, p.M112V, and p.K297X) were discovered to be associated with atypical parkinsonism. Here, we apply quantitative proteomics to provide a detailed description of the retromer interac- tome. By establishing a comparative proteomic methodology, we identify how this interactome is perturbed in atypical parkinsonism-associated VPS26A mutants. In particular, we describe a selective defect in the association of VPS26A (p.K297X) with the SNX27 cargo adaptor. By showing how a retromer mutant leads to altered endosomal sorting of specific PDZ ligand–containing cargo proteins, we reveal a new mechanism for perturbed endosomal cargo sorting in atypical parkinsonism. Introduction Retromer is a highly conserved heterotrimer of VPS29, homologue (WASH) complex (McGough et al., 2014a; Za- VPS35, and VPS26.
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Structural Basis of Mammalian Mucin Processing by the Human Gut O
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Atypical Parkinsonism–Associated Retromer Mutant Alters Endosomal Sorting of Specific Cargo Proteins
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VPS26A Polyclonal Antibody Gene Alias: FLJ12930, HB58, Hbeta58, PEP8A, VPS26
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Sequence-Dependent Cargo Recognition by SNX-Bars Mediates Retromer-Independent Transport of CI-MPR
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Dr. Alan M. Jones 1 Address
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