VPS26A (1-327, His-Tag) Human Protein – AR50466PU-N | Origene

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VPS26A (1-327, His-Tag) Human Protein – AR50466PU-N | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for AR50466PU-N VPS26A (1-327, His-tag) Human Protein Product data: Product Type: Recombinant Proteins Description: VPS26A (1-327, His-tag) human recombinant protein, 0.1 mg Species: Human Expression Host: E. coli Tag: His-tag Predicted MW: 40.6 kDa Concentration: lot specific Purity: >85% by SDS - PAGE Buffer: Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.15M NaCl Preparation: Liquid purified protein Protein Description: Recombinant human VPS26A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Stability: Shelf life: one year from despatch. RefSeq: NP_001030337 Locus ID: 9559 UniProt ID: O75436 Cytogenetics: 10q22.1 Synonyms: HB58; Hbeta58; PEP8A; VPS26 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 VPS26A (1-327, His-tag) Human Protein – AR50466PU-N Summary: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Product images: This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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Atypical Parkinsonism–Associated Retromer Mutant Alters Endosomal Sorting of Specific Cargo Proteins
JCB: Report Atypical parkinsonism–associated retromer mutant alters endosomal sorting of specific cargo proteins Kirsty J. McMillan,1 Matthew Gallon,1* Adam P. Jellett,1* Thomas Clairfeuille,3 Frances C. Tilley,1 Ian McGough,1 Chris M. Danson,1 Kate J. Heesom,2 Kevin A. Wilkinson,1 Brett M. Collins,3 and Peter J. Cullen1 1School of Biochemistry and 2Proteomics Facility, School of Biochemistry, University of Bristol, Bristol BS8 1TD, England, UK 3Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland 4072, Australia The retromer complex acts as a scaffold for endosomal protein complexes that sort integral membrane proteins to vari- ous cellular destinations. The retromer complex is a heterotrimer of VPS29, VPS35, and VPS26. Two of these paral- ogues, VPS26A and VPS26B, are expressed in humans. Retromer dysfunction is associated with neurodegenerative disease, and recently, three VPS26A mutations (p.K93E, p.M112V, and p.K297X) were discovered to be associated with atypical parkinsonism. Here, we apply quantitative proteomics to provide a detailed description of the retromer interac- tome. By establishing a comparative proteomic methodology, we identify how this interactome is perturbed in atypical parkinsonism-associated VPS26A mutants. In particular, we describe a selective defect in the association of VPS26A (p.K297X) with the SNX27 cargo adaptor. By showing how a retromer mutant leads to altered endosomal sorting of specific PDZ ligand–containing cargo proteins, we reveal a new mechanism for perturbed endosomal cargo sorting in atypical parkinsonism. Introduction Retromer is a highly conserved heterotrimer of VPS29, homologue (WASH) complex (McGough et al., 2014a; Za- VPS35, and VPS26.
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Atypical Parkinsonism–Associated Retromer Mutant Alters Endosomal Sorting of Specific Cargo Proteins
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