RESEARCH ARTICLE An Integrative Approach for Understanding Diversity in the Punctelia rudecta Species Complex (Parmeliaceae, Ascomycota) David Alors1*, H. Thorsten Lumbsch2, Pradeep K. Divakar1, Steven D. Leavitt2, Ana Crespo1 1 Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal s/n, Madrid, Spain, 2 Science and Education, Field Museum, Chicago, Illinois, United States of America *
[email protected] Abstract High levels of cryptic diversity have been documented in lichenized fungi, especially in Par- OPEN ACCESS meliaceae, and integrating various lines of evidence, including coalescent-based species delimitation approaches, help establish more robust species circumscriptions. In this study, Citation: Alors D, Lumbsch HT, Divakar PK, Leavitt we used an integrative taxonomic approach to delimit species in the lichen-forming fungal SD, Crespo A (2016) An Integrative Approach for Understanding Diversity in the Punctelia rudecta genus Punctelia (Parmeliaceae), with a particular focus on the cosmopolitan species Species Complex (Parmeliaceae, Ascomycota). P. rudecta. Nuclear, mitochondrial ribosomal DNA and protein-coding DNA sequences PLoS ONE 11(2): e0146537. doi:10.1371/journal. were analyzed in phylogenetic and coalescence-based frameworks. Additionally, morpho- pone.0146537 logical, ecological and geographical features of the sampled specimens were evaluated. Editor: William Oki Wong, Institute of Botany, CHINA Five major strongly supported monophyletic clades were recognized in the genus Punctelia, Received: May 8, 2015 and each clade could be characterized by distinct patterns in medullary chemistry. Punctelia Accepted: December 18, 2015 rudecta as currently circumscribed was shown to be polyphyletic. A variety of empirical spe- cies delimitation methods provide evidence for a minimum of four geographically isolated Published: February 10, 2016 species within the nominal taxon Punctelia rudecta, including a newly described saxicolous Copyright: © 2016 Alors et al.