For ResearchUseOnly. NotForUseInDiagnostic Procedures. © 2013 Cell Signaling Technology, Inc. XP® and Cell Signaling Technology® are trademarks of Cell Signaling Technology, Inc. Store at -20°C Dg—dog Species Cross-Reactivity Key: Applications Key: Background References: protein. ing toresiduessurroundingPro73ofhumanSMARCA1 by immunizinganimalswithasyntheticpeptidecorrespond Source/Purification: Monoclonalantibodyisproduced recognizes endogenouslevelsoftotalSMARCA1protein. Specificity/Sensitivity: SMARCA1(D4Q7V)RabbitmAb with regulationofneurulation(4). neural tubedefectexencephaly, linkingtheCERFcomplex an interactingpartnerofSMARCA1,isassociatedwiththe (7). DisruptionoftheCERFcomplexbydeletionCECR2, in thedevelopmentofgermlayersmouseembryo ectoderm tissuelineages,suggestingaroleforSMARCA1 quired forproperdevelopmentofmesoderm,endoderm,and (6). ThelargestsubunitoftheNURFcomplex,BPTF, isre- and expressionofgenesimportantforTcelldevelopment of Tcellsfromthymocytesbyregulatingchromatinstructure (3). NURFisalsothoughttobeinvolvedinthematuration are involvedinneuronaldevelopmentthemid-hindbrain outgrowth andregulationofEngrailedhomeoticgenesthat important roleinneuronalphysiologybypromotingneurite factor (CERF)complexes(3-5).TheNURFcomplexplaysan modeling factor(NURF)andCECR2-containingremodeling SMARCA1 isthecatalyticsubunitofnucleosomere shown toremodelandalternucleosomespacing complexes werefirstidentifiedinDrosophilaandhavebeen mammalian genome(1).TheISWIchromatinremodeling logs oftheISWI(imitationswitch)ATPases encodedbythe Background: SMARCA1(SNF2L)isoneofthetwoortho-

(7)  (6)  (5)  (4)  (3)  (2)  (1)  #12483 0.1% Tween-20 at4°Cwithgentle shaking,overnight. IMPORTANT: Forwesternblots,incubatemembrane withdilutedantibodyin5%w/vBSA,1XTBS, Landry, J.etal.(2008)PLoSGenet4,e1000241. Landry, J.W. etal.(2011)GenesDev25,275-86. Ho, L.andCrabtree,G.R.(2010)Nature463,474-84. Banting, G.S.etal.(2005)HumMolGenet14,513-24. Barak, O.etal.(2003)EMBOJ22,6089-100. Erdel, F. andRippe,K.(2011)FEBSJ278,3608-18. Lazzaro, M.A.andPicketts,D.J.(2001)JNeurochem 77, 1145-56. n SMARCA1 (D4Q7V)RabbitmAb 3 Endogenous Applications Pg—pig  (10 westernblots) 100 µl W, IP Sc—S. cerevisiae W—Western H—human Species Cross-Reactivity* IP—Immunoprecipitation Ce—C. elegans H, Mk M—mouse

in vitro(2). Hr—horse R—rat - IHC—Immunohistochemistry - Hm—hamster using Rabbit(DA1E)IgGXP Immunoprecipitation ofSMARCA1fromPANC-1 cellextracts Rabbit mAb. Western blotanalysiswasperformed usingSMARCA1(D4Q7V) SMARCA1 (D4Q7V)RabbitmAb(lane3).Lane1is10%input. SMARCA1 (D4Q7V)RabbitmAb. Western Blotanaylsisofextractsfromvariouscelllinesusing 100 140 200 kDa Molecular Wt. 20 30 40 50 60 80 100 140 200 kDa 60 80 130 kDa All—all species expected

Mk—monkey LN18 – – 1

ChIP—Chromatin Immunoprecipitation SW620 – + 2

HeLa 3 + – ® Mi—mink Rabbit IgG** IsotypeControl#3900(lane2)or SMARCA1 (D4Q7V)RabbitmAb XP Rabbit (DA1E)mAbIgG HT-29 New 03/13 SMARCA1 IgG Isotype ® IsotypeControl Saos-2 Species enclosed inparenthesesarepredicted toreactbasedon100% homology. OVCAR8 C—chicken

COS-7 SMARCA1 Dm—D. melanogaster IF—Immunofluorescence Swiss-Prot Acc.#P28370 Entrez-Gene ID#6594 of recommended complementary products. of recommendedcomplementary Please visitwww.cellsignal.com foracompletelisting for thisproductatwww.cellsignal.com. For productspecificprotocolspleaseseethewebpage Immunoprecipitation 1:100 Western blotting Recommended AntibodyDilutions: ** *Species cross-reactivityisdeterminedbywesternblot. sodium azide.Storeat–20°C.Donotaliquottheantibody. mM NaCl,100µg/mlBSA,50%glycerolandlessthan0.02% Storage: Suppliedin10mMsodiumHEPES(pH7.5),150 detect thisantibody. antibodiesmustbeusedto Anti-rabbit secondary X—Xenopus Support Orders Web F—Flow cytometry Z—zebrafish n n n www.cellsignal.com [email protected] 877-678-TECH (8324) [email protected] 877-616-CELL (2355) E-P—ELISA-Peptide B—bovine 1:1000