SMARCA1 (D4Q7V) Rabbit mAb Store at -20°C 3 n 100 µl Orders n 877-616-CELL (2355) (10 western blots) [email protected] Support n 877-678-TECH (8324) [email protected] Web n www.cellsignal.com New 03/13 #12483 For Research Use Only. Not For Use In Diagnostic Procedures. Entrez-Gene ID #6594 Swiss-Prot Acc. #P28370 Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 Applications Species Cross-Reactivity* Molecular Wt. Isotype mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% W, IP H, Mk 130 kDa Rabbit IgG** sodium azide. Store at –20°C. Do not aliquot the antibody. Endogenous *Species cross-reactivity is determined by western blot. Background: SMARCA1 (SNF2L) is one of the two ortho- ** Anti-rabbit secondary antibodies must be used to logs of the ISWI (imitation switch) ATPases encoded by the detect this antibody. kDa LN18 SW620 HeLa HT-29 Saos-2 OVCAR8COS-7 mammalian genome (1). The ISWI chromatin remodeling Recommended Antibody Dilutions: complexes were first identified in Drosophila and have been 200 140 Western blotting 1:1000 shown to remodel and alter nucleosome spacing in vitro (2). SMARCA1 100 Immunoprecipitation 1:100 SMARCA1 is the catalytic subunit of the nucleosome re- 80 modeling factor (NURF) and CECR2-containing remodeling For product specific protocols please see the web page 60 factor (CERF) complexes (3-5). The NURF complex plays an 50 for this product at www.cellsignal.com. important role in neuronal physiology by promoting neurite 40 Please visit www.cellsignal.com for a complete listing outgrowth and regulation of Engrailed homeotic genes that 30 of recommended complementary products. are involved in neuronal development in the mid-hindbrain (3). NURF is also thought to be involved in the maturation 20 of T cells from thymocytes by regulating chromatin structure and expression of genes important for T cell development Western Blot anaylsis of extracts from various cell lines using (6). The largest subunit of the NURF complex, BPTF, is re- SMARCA1 (D4Q7V) Rabbit mAb. quired for proper development of mesoderm, endoderm, and ectoderm tissue lineages, suggesting a role for SMARCA1 in the development of the germ layers in mouse embryo (7). Disruption of the CERF complex by deletion of CECR2, kDa 1 2 3 an interacting partner of SMARCA1, is associated with the 200 neural tube defect exencephaly, linking the CERF complex 140 with regulation of neurulation (4). SMARCA1 Specificity/Sensitivity: SMARCA1 (D4Q7V) Rabbit mAb 100 recognizes endogenous levels of total SMARCA1 protein. 80 Source/Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide correspond- ing to residues surrounding Pro73 of human SMARCA1 protein. IgG 60 Background References: (1) Lazzaro, M.A. and Picketts, D.J. (2001) J Neurochem – + – Rabbit (DA1E) mAb IgG 77, 1145-56. XP® Isotype Control – – + SMARCA1 (D4Q7V) Rabbit mAb (2) Erdel, F. and Rippe, K. (2011) FEBS J 278, 3608-18. (3) Barak, O. et al. (2003) EMBO J 22, 6089-100. Immunoprecipitation of SMARCA1 from PANC-1 cell extracts using Rabbit (DA1E) IgG XP® Isotype Control #3900 (lane 2) or (4) Banting, G.S. et al. (2005) Hum Mol Genet 14, 513-24. SMARCA1 (D4Q7V) Rabbit mAb (lane 3). Lane 1 is 10% input. (5) Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84. Western blot analysis was performed using SMARCA1 (D4Q7V) Rabbit mAb. (6) Landry, J.W. et al. (2011) Genes Dev 25, 275-86. are trademarks of Cell Signaling Technology, Inc. are trademarks of Cell Signaling Technology, ® (7) Landry, J. et al. (2008) PLoS Genet 4, e1000241. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Applications Key: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide and Cell Signaling Technology ® Species Cross-Reactivity Key: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine © 2013 Cell Signaling Technology, Inc. © 2013 Cell Signaling Technology, XP Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—horse All—all species expected Species enclosed in parentheses are predicted to react based on 100% homology..