bioRxiv preprint doi: https://doi.org/10.1101/2021.06.30.450495; this version posted July 1, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Inverse Density of Iba1 and Glutamine Synthetase Expressing Glia in Rat Inferior Colliculus Running title: Iba1 & Glutamine Synthetase in Inferior Colliculi Author names and affiliations: Llwyd David Orton Department of Life Sciences, Manchester Metropolitan University, Manchester, M1 5GD, UK Corresponding author: Llwyd Orton:
[email protected]; +44 161 247 2483 Keywords: Auditory, Anatomy, Microglia, Astrocytes, Immunohistochemistry Contributions (CRediT): LDO: Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, review and editing. Acknowledgements: The author thanks Dave Maskew, Megan Ryan and Rosie Mitchell for their technical support. Conflicting interests: The author declares no competing interests. bioRxiv preprint doi: https://doi.org/10.1101/2021.06.30.450495; this version posted July 1, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Abstract Microglia and astrocytes undertake numerous essential roles in nervous systems but we know little of their anatomical distribution within numerous nuclei. In the principal nuclei of the mammalian auditory midbrain, the inferior colliculi (IC), the cellular density and relative distribution of glutamate synthetase (GS) expressing astrocytes and ionized calcium-binding adapter molecule 1 (Iba1) expressing microglia is unknown.