Carnivorous Plant Newsletter Vol. 40 No. 4 December 2011
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The Terrestrial Carnivorous Plant Utricularia Reniformis Sheds Light on Environmental and Life-Form Genome Plasticity
International Journal of Molecular Sciences Article The Terrestrial Carnivorous Plant Utricularia reniformis Sheds Light on Environmental and Life-Form Genome Plasticity Saura R. Silva 1 , Ana Paula Moraes 2 , Helen A. Penha 1, Maria H. M. Julião 1, Douglas S. Domingues 3, Todd P. Michael 4 , Vitor F. O. Miranda 5,* and Alessandro M. Varani 1,* 1 Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP—Universidade Estadual Paulista, Jaboticabal 14884-900, Brazil; [email protected] (S.R.S.); [email protected] (H.A.P.); [email protected] (M.H.M.J.) 2 Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Bernardo do Campo 09606-070, Brazil; [email protected] 3 Departamento de Botânica, Instituto de Biociências, UNESP—Universidade Estadual Paulista, Rio Claro 13506-900, Brazil; [email protected] 4 J. Craig Venter Institute, La Jolla, CA 92037, USA; [email protected] 5 Departamento de Biologia Aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP—Universidade Estadual Paulista, Jaboticabal 14884-900, Brazil * Correspondence: [email protected] (V.F.O.M.); [email protected] (A.M.V.) Received: 23 October 2019; Accepted: 15 December 2019; Published: 18 December 2019 Abstract: Utricularia belongs to Lentibulariaceae, a widespread family of carnivorous plants that possess ultra-small and highly dynamic nuclear genomes. It has been shown that the Lentibulariaceae genomes have been shaped by transposable elements expansion and loss, and multiple rounds of whole-genome duplications (WGD), making the family a platform for evolutionary and comparative genomics studies. To explore the evolution of Utricularia, we estimated the chromosome number and genome size, as well as sequenced the terrestrial bladderwort Utricularia reniformis (2n = 40, 1C = 317.1-Mpb). -
Hunters Or Farmers? Microbiome Characteristics Help Elucidate the Diet Composition in an Aquatic Carnivorous Plant
Sirová et al. Microbiome (2018) 6:225 https://doi.org/10.1186/s40168-018-0600-7 RESEARCH Open Access Hunters or farmers? Microbiome characteristics help elucidate the diet composition in an aquatic carnivorous plant Dagmara Sirová1,2*† ,Jiří Bárta2†, Karel Šimek1,2, Thomas Posch3,Jiří Pech2, James Stone4,5, Jakub Borovec1, Lubomír Adamec6 and Jaroslav Vrba1,2 Abstract Background: Utricularia are rootless aquatic carnivorous plants which have recently attracted the attention of researchers due to the peculiarities of their miniaturized genomes. Here, we focus on a novel aspect of Utricularia ecophysiology—the interactions with and within the complex communities of microorganisms colonizing their traps and external surfaces. Results: Bacteria, fungi, algae, and protozoa inhabit the miniature ecosystem of the Utricularia trap lumen and are involved in the regeneration of nutrients from complex organic matter. By combining molecular methods, microscopy, and other approaches to assess the trap-associated microbial community structure, diversity, function, as well as the nutrient turn-over potential of bacterivory, we gained insight into the nutrient acquisition strategies of the Utricularia hosts. Conclusions: We conclude that Utricularia traps can, in terms of their ecophysiological function, be compared to microbial cultivators or farms, which center around complex microbial consortia acting synergistically to convert complex organic matter, often of algal origin, into a source of utilizable nutrients for the plants. Keywords: Algae, Bacteria, Ciliate bacterivory, Digestive mutualism, Fungi, Herbivory, Nutrient turnover, Plant– microbe interactions, Protists, Utricularia traps Background microbial communities clearly play a significant role in Plant-associated microorganisms have long been plant ecophysiology, but many of the underlying mech- recognized as key partners in enhancing plant nutrient anisms governing these looser associations still remain acquisition, mitigating plant stress, promoting growth, unexplored [2]. -
Brief Information About the Species Status of Utricularia Cornigera Studnicˇka
Technical Refereed Contribution Brief information about the species status of Utricularia cornigera Studnicˇka Miloslav studnicˇka • Liberec Botanic Gardens • Purkynˇova 630/1 • CZ-460 01 Liberec • Czech Republic • [email protected] Keywords: Utricularia cornigera, hybrid, heterosis, apomixis Abstract: The carnivorous plant Utricularia cornigera Studnicˇka was described in 2009, but author- ities of the International Carnivorous Plant Society published an opinion that it is not a true species, but only a natural hybrid of U. reniformis and U. nelumbifolia. The role of heterosis is discussed, because U. cornigera is much larger than both theoretical parents. Seedlings, the very characteristic feature of bladderworts (Utricularia), are different in all the bladderworts described, that is, in the named species and in artificial hybrids of U. nelumbifolia and U. reniformis. No support for the hypothesis supposing a hybrid origin of U. cornigera was found. Introduction Recently a hypothesis appeared that Utricularia cornigera Studnicˇ ka could be a hybrid of U. nelum- bifolia Gardn. × U. reniformis St.Hil. (Schlauer 2011; Fleischmann 2012). Consequentially, the new species was rejected from the Carnivorous Plant Database (Schlauer 2011). Nevertheless it was accepted in the International Plant Name Index (IPNI 2005). This article presents the results of new experiments with artificial crossings of both theoretical parents proposed by the authors. The manner of germination and specifically the appearance of the seedlings are crucial phenomena in the life strategy of bladderworts. In the Utricularia species from the section Iperua there are two different ways of germination: either by floating seedlings (e.g. U. cornigera, U. nelumbifolia), or by terrestrial seedlings (e.g. -
Building Capacity for Plant Conservation – the Role of Botanic Gardens Volume 10 • Number 1 EDITORIAL SARA OLDFIELD 02
Journal of Botanic Gardens Conservation International Volume 10 • Number 1 • January 2013 Building capacity for plant conservation – the role of botanic gardens Volume 10 • Number 1 EDITORIAL SARA OLDFIELD 02 EDITORS 21 03 BUILDING CAPACITY THROUGH TEACHING ESSENTIAL SKILLS Suzanne Sharrock Sara Oldfield THE ROLE OF BOTANIC GARDENS IN LAURA COHEN AND LEIGH MORRIS Director of Global Secretary General Programmes BUILDING CAPACITY FOR PLANT Cover Photo : Reintroduction of “pata de elefante” CONSERVATION MARIANA CHAVEZ Beaucarnea gracilis in Cuicatlán, Oaxaca, México. A collaborative project between the Botanical Garden and AND SUZANNE SHARROCK the local people (Archive of the Jardín Botanico of the Universidad Nacional Autonoma de Mexico) Design : Seascape www.seascapedesign.co.uk BGjournal is published by Botanic Gardens Conservation International (BGCI) . It is published twice a year and is sent to all BGCI members. Membership is open to all interested individuals, institutions and organisations that support the aims of BGCI (see inside back cover for Membership application form). 08 25 Further details available from: THE UNIVERSITY OF WASHINGTON • Botanic Gardens Conservation International, Descanso BOTANIC GARDEN AND RESTORATION BUILDING CAPACITY AND FACILITATING House, 199 Kew Road, Richmond, Surrey TW9 3BW UK. Tel: +44 (0)20 8332 5953, Fax: +44 (0)20 8332 5956 ECOLOGY KERN EWING AND NETWORKS FOR PLANT E-mail: [email protected], www.bgci.org SARAH REICHARD CONSERVATION: KEW’S ON-GOING • BGCI-Russia, c/o Main Botanical Gardens, COMMITMENT -
Floral Micromorphology and Nectar Composition of the Early Evolutionary Lineage Utricularia (Subgenus Polypompholyx, Lentibulariaceae)
Protoplasma https://doi.org/10.1007/s00709-019-01401-2 ORIGINAL ARTICLE Floral micromorphology and nectar composition of the early evolutionary lineage Utricularia (subgenus Polypompholyx, Lentibulariaceae) Bartosz J. Płachno1 & Małgorzata Stpiczyńska 2 & Piotr Świątek3 & Hans Lambers4 & Gregory R. Cawthray4 & Francis J. Nge5 & Saura R. Silva6 & Vitor F. O. Miranda6 Received: 1 April 2019 /Accepted: 4 June 2019 # The Author(s) 2019 Abstract Utricularia (Lentibulariaceae) is a genus comprising around 240 species of herbaceous, carnivorous plants. Utricularia is usually viewed as an insect-pollinated genus, with the exception of a few bird-pollinated species. The bladderworts Utricularia multifida and U. tenella are interesting species because they represent an early evolutionary Utricularia branch and have some unusual morphological characters in their traps and calyx. Thus, our aims were to (i) determine whether the nectar sugar concentrations andcompositioninU. multifida and U. tenella are similar to those of other Utricularia species from the subgenera Polypompholyx and Utricularia, (ii) compare the nectary structure of U. multifida and U. tenella with those of other Utricularia species, and (iii) determine whether U. multifida and U. tenella use some of their floral trichomes as an alternative food reward for pollinators. We used light microscopy, histochemistry, and scanning and transmission electron microscopy to address those aims. The concentration and composition of nectar sugars were analysed using high-performance liquid chroma- tography. In all of the examined species, the floral nectary consisted of a spur bearing glandular trichomes. The spur produced and stored the nectar. We detected hexose-dominated (fructose + glucose) nectar in U. multifida and U. tenella as well as in U. -
VCPS Mar 06 Journal No 79
ISSN 1033-6966 VICTORIAN CARNIVOROUS PLANT SOC IETY Inc. March 2006 No. 79 P. immaculata x emarginata Nepenthes x allardii Utricularia nelumbifolia Dionaea muscipula “Goliath” Drosera parvula ssp sargentii Sarracenia flava var. rugelii Drosera praefolia D. whittakerii ssp whittakerii D. whittakerii ssp whittakerii VICTORIAN CARNIVOROUS VICTORIAN CARNIVOROUS PLANT SOC IETY Inc. PLANT SOC IETY Inc. Annual Subscriptions Issue No. 79 March 2006 Australian membership $20.00 Office Bearers: July 2005 – June 2006 Overseas membership $20.00 Payment from overseas must be in Australian dollars. President Stephen Fretwell All cheques or money orders should be made payable to the Victorian Carnivorous Plant Society Inc (VCPS). Vice President Sean Spence Payment by credit card is NOT available at the time of this journal issue. General Secretary Paul Edwards Correspondence Minutes Secretary Sean Spence Please forward all correspondence regarding subscription, change of address, Other Publications Gordon Ohlenrott articles for the journal and back issues to: The Secretary VCPS Journal Editor Stephen Fretwell P.O. Box 201 SOUTH YARRA 3141. Assistant Journal Editor George Caspar AUSTRALIA Internet Co-ordinator Paul Edwards Journal articles, in MS-Word, ready for publication, may be Emailed to the Editor or Secretary. Treasurer Ken Neal Librarian Andrew Gibbons Meetings Seedbank Administrator George Caspar Most VCPS meetings are held in the hall at the rear of the Pilgrim Uniting Church on the corner of Bayview Road and Montague Street, Yarraville – Melway map reference Hardware Co-ordinator Andre Cleghorn 41K7. These meetings are on the fourth Wednesday of the month at 8 PM. However, some meetings may be at the home of members during a weekend. -
The Discovery of Drosera Magnifica, the “Facebook Sundew”
Deep in the web, up in the hills: the discovery of Drosera magnifica, the “Facebook sundew” Paulo Minatel Gonella • Lab. Sistemática Vegetal • Instituto de Biociências • Universidade de São Paulo • Brazil • [email protected] Social media are part of the daily life in the modern society: we follow the news on Twitter, share funny gifs on Tumblr, post selfies on Facebook, spread scientific papers on ResearchGate, and share experiences and trade plants in the discussion forums. Among all possible connections achieved by the social media, one that has always captivated me is the bridge they created to connect professional biologists to “citizen scientists”. From the age of the forums to Facebook, I’ve been fol- lowing with keen interest posts with photos of carnivorous plants in their natural habitats in Brazil and abroad, and many new regional records were found that way, and even species discoveries and re-discoveries. The long-lost Drosera ascendens A.St.-Hil. was rediscovered thanks to social media, as it was the discovery of the peculiar D. chimaera Gonella & Rivadavia. Both findings were made possible thanks to photos posted by the carnivorous plant enthusiast Adilson Peres in the Brazilian carnivo- rous plant forum back in 2010. His photos and the information he provided on the location of his encounters enabled us to collect and study these plants, and finally solve the taxonomy of the group (see Gonella et al. 2014). Cases of discoveries of new species thanks to social media are not restrict- ed to carnivorous plants, though. The enormous palm Tahina spectabilis J.Dransf. -
A New Species of Utricularia Sect. Orchidioides (Lentibulariaceae) from the Amotape-Huancabamba Zone of Northern Peru
DARWINIANA, nueva serie 9(2): 299-311. 2021 Versión de registro, efectivamente publicada el 27 de agosto de 2021 DOI: 10.14522/darwiniana.2021.92.955 ISSN 0011-6793 impresa - ISSN 1850-1699 en línea A NEW SPECIES OF UTRICULARIA SECT. ORCHIDIOIDES (LENTIBULARIACEAE) FROM THE AMOTAPE-HUANCABAMBA ZONE OF NORTHERN PERU Tilo Henning1 ID , Joshua P. Allen2 ID & Eric F. Rodríguez Rodríguez3 ID 1 Leibniz Centre for Agricultural Landscape Research (ZALF), Eberswalder Str. 84, 15374, Müncheberg, Germany; [email protected] (author for correspondence). 2 Comunidad Campesina Maska S/N, Pisac, Peru. 3 Herbarium Truxillense (HUT), Universidad Nacional de Trujillo, Jr. San Martín 392, Trujillo, Peru. Abstract. Henning, T.; J. P. Allen & E. F. Rodríguez Rodríguez. 2021. A new species of Utricularia Sect. Orchidioides (Lentibulariaceae) from the Amotape-Huancabamba Zone of North Peru. Darwiniana, nueva serie 9(2): 299-311. A new species of Utricularia Section Orchidioides: Utricularia amotape-huancabambensis sp. nov. (Lentibulariaceae), endemic to Northern Peru is described and illustrated. It is known from two populations so far, both located in the Province Bongará, Dpto. Amazonas at ca. 2200 m altitude. The new species inhabits the páramo of the low white sandstone plateaus, an extension of the southern branches of the Condor mountain range in the southeastern part of the Amotape-Huancabamba phytogeographic zone. A diagnosis, description, etymology and an identification key covering the Andean and selected similar species of the section are presented. The distribution, habitat, ecology, associated flora, preliminary conservation status, an observed hybridization with sympatric U. unifolia and the affinities of the new species with other related taxa are discussed. -
Structural Features of Carnivorous Plant (Genlisea, Utricularia)
1 Article – Supplementary Materials 2 Structural features of carnivorous plant (Genlisea, 3 Utricularia) tubers as abiotic stress resistance organs 4 Bartosz J. Płachno 1,*, Saura R. Silva 2, Piotr Świątek 3, Kingsley W. Dixon 4, Krzystof Lustofin 1, 5 Guilherme C. Seber 2 and Vitor F. O. Miranda 2 6 1 Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian 7 University in Kraków, Gronostajowa 9 St. 30-387 Cracow, Poland; [email protected] 8 (K.L.) 9 2 São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Laboratory of Plant 10 Systematics, Jaboticabal, CEP 14884-900, SP, Brazil; [email protected] (S.R.S); [email protected] 11 (G.C.S.); [email protected] (V.F.O.M.) 12 3 Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University 13 of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice; [email protected] 14 4 School of Molecular and Life Sciences, Curtin University, Kent Street, Bentley, Perth, Western Australia 15 6102, Australia; [email protected] 16 17 * Correspondence: [email protected] 18 19 20 Table S1. Data used for the phylogenetic analyses. “-” denotes missing data. Pinguicula species were 21 used as outgroup. Species matK/trnK rbcL Genlisea africana FN641702 - Genlisea aurea NC037078 NC037078 Genlisea barthlottii FN641704 - Genlisea filiformis NC037079 NC037079 Genlisea glabra FN641692 - Genlisea glandulosissima FN641700 - Genlisea guianensis FN641696 AY128631 Genlisea hispidula FN641705 - Genlisea lobata FN641711 - Genlisea margaretae HG530134 HG530134 Genlisea pygmaea NC037080 NC037080 Genlisea repens NC037081 NC037081 Genlisea roraimensis AF531817 - Genlisea sanariapoana FN641698 - Int. -
University of Pisa
University of Pisa Research Doctorate in Biology XXVIII Cycle BIOSYSTEMATICS OF EUROPEAN SPECIES OF CARNIVOROUS GENUS UTRICULARIA (LAMIALES, ANGIOSPERMS) DOCTORAL THESIS PhD Student: Giovanni Astuti Supervisor: Lorenzo Peruzzi SSD BIO/02 Systematic Botany Academic Years 2012-2015 Pisa, January 2016 Copyright © The author 2016 1 In loving memory of my father Mario “…it’s just a ride…” Bill Hicks 2 TABLE OF CONTENTS ABSTRACT 5 INTRODUCTION 6 The carnivorous plants 6 The family Lentibulariaceae 7 Lentibulariaceae as model organisms in genomic studies 9 The genus Utricularia 12 Distribution and habitats 12 Systematics and evolution 14 General morphology 16 Utricularia prey spectra 19 European species of Utricularia 20 Species description 23 Utricularia intermedia aggr. 23 Utricularia minor aggr. 30 Utricularia vulgaris aggr. 35 Taxonomic and Systematic problems 42 Objectives of the thesis 45 The use and utility of Geometric morphometrics 46 DNA Barcoding approach 48 MATERIAL & METHODS 51 General sampling 51 ‘Traditional’ morphometric analysis 51 Geometric morphometric analysis 53 Molecular analysis 56 DNA extraction, amplification and sequencing 56 DNA Barcoding approach 57 Splits and phylogenetic networks 58 Phylogenetic trees 59 RESULTS 62 3 ‘Traditional’ morphometric analysis 62 Geometric morphometric analysis 66 Shape 66 All species 66 Utricularia intermedia aggregate 70 Utricularia minor aggregate 70 Utricularia vulgaris aggregate 71 Molecular analysis 78 DNA Barcoding 78 Phylogenetic relationships 79 DISCUSSION 82 Morphometric analysis 82 Molecular analysis 85 CONCLUSIONS 88 IDENTIFICATION KEY 91 ACKNOWLEDGEMENTS 92 REFERENCES 93 APPENDIX I 108 APPENDIX II 110 PAPERS AND ABSTRACTS (last three years) 111 ACTIVITIES DONE ABROAD 114 4 ABSTRACT Utricularia is a genus of carnivorous plants catching its preys using small traps. -
The Structure and Occurrence of a Velum in Utricularia Traps (Lentibulariaceae)
ORIGINAL RESEARCH published: 22 March 2019 doi: 10.3389/fpls.2019.00302 The Structure and Occurrence of a Velum in Utricularia Traps (Lentibulariaceae) Bartosz J. Płachno1*, Piotr S´ wia˛ tek2, Vitor F. O. Miranda3 and Piotr Stolarczyk4 1 Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University in Kraków, Cracow, Poland, 2 Department of Animal Histology and Embryology, University of Silesia in Katowice, Katowice, Poland, 3 Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, Departamento de Biologia Aplicada à Agropecuária, UNESP–Universidade Estadual Paulista, São Paulo, Brazil, 4 Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, University of Agriculture in Kraków, Cracow, Poland Bladderworts (Utricularia, Lentibulariaceae, Lamiales) are carnivorous plants that form small suction traps (bladders) for catching invertebrates. The velum is a cuticle structure Edited by: that is produced by specialized trichomes of the threshold pavement epithelium. It is Simon Poppinga, University of Freiburg, Germany believed that the velum together with the mucilage seals the free edge of the trap door Reviewed by: and that it is necessary for correct functioning of the trap. However, recently, some authors Lubomir Adamec, have questioned the occurrence of a velum in the traps of the Utricularia from the various Institute of Botany of the Academy of Sciences of the Czech Republic, sections. The main aim of this study was to confirm whether velum occurs in the traps Czechia of the Utricularia species from the subgenera Polypompholyx, Bivalvaria, and Utricularia. Rolf Rutishauser, The 15 species were examined from subg. Polypompholyx, subg. Bivalvaria, and subg. University of Zurich, Switzerland Ulrike K. -
Nectar Trichome Structure of Aquatic Bladderworts from the Section Utricularia (Lentibulariaceae) with Observation of Flower Visitors and Pollinators
Protoplasma (2018) 255:1053–1064 https://doi.org/10.1007/s00709-018-1216-2 ORIGINAL ARTICLE Nectar trichome structure of aquatic bladderworts from the section Utricularia (Lentibulariaceae) with observation of flower visitors and pollinators Bartosz J. Płachno1 & Małgorzata Stpiczyńska2 & Lubomír Adamec3 & Vitor Fernandes Oliveira Miranda4 & Piotr Świątek5 Received: 20 November 2017 /Accepted: 26 January 2018 /Published online: 5 February 2018 # The Author(s) 2018. This article is an open access publication Abstract In Utricularia, the flower spur is a nectary and in this organ, nectar is produced and stored. This study aimed to examine the structure of the nectary trichomes in four Utricularia species (Utricularia vulgaris L., U. australis R.Br., U. bremii Heer and U. foliosa L.) from the generic section Utricularia. We have investigated whether species with different spur morphology had similar spur anatomy and nectary trichome structure. In Utricularia flowers, nectar is produced by spur capitate trichomes (sessile or stalked). Our results showed that regardless of the various spur morphology, trichomes have similar architecture and ultra- structure. Head cells of these trichomes are transfer cells with an eccrine nectar secretion. Examined species differed in the micromorphology of papillae in spurs. The fly Eristalis tenax was found to be a pollinator of U. vulgaris. Small Halictidae bees seem to be pollinators of U. foliosa. Keywords Bladderwort . Sect. Utricularia . Lentibulariaceae . Carnivorous plant . Floral micromorphology . Nectar . Spur . Pollination . Nectary ultrastructure . Entomophily . Trichomes Introduction Angraecum sesquipedale,Darwin(1862) proposed the coevo- lution of the length of a nectar spur and its pollinator’stongue. In flowering plants, typical flower spurs are tubular out- Nectary spur characters are strongly associated with a type of growths of perianth organs which contain nectar for pollina- pollinator, and this organ has evolved multiple times across tors.