ECH1 Pre-Design Chimera Rnai

ECH1 Pre-design Chimera RNAi

Catalog # : H00001891-R01 規格 : [ 10 nmol ] [ 20 nmol ] List All

Specification Application Image

Product Homo sapiens enoyl Coenzyme A hydratase 1, peroxisomal (ECH1), RNAi Knockdown Description: mRNA.

Reactivity: Human

Supplied DEPC water Product:

Target Refseq: NM_001398

Storage Store at -20°C, do not exceed 4 - 5 freeze-thaw cycles to ensure Instruction: product integrity.

Note: Position of the Chimera RNAi.

Publication Reference 1. dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference. Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91. 2. Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect. Ui-Tei K, Naito Y, Zenno S, Nishi K, Yamato K, Takahashi F, Juni A, Saigo K.Nucleic Acids Res. 2008 Apr;36(7):2136-51. Epub 2008 Feb 11. 3. Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference. Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K.Nucleic Acids Res. 2004 Feb 9;32(3):936-48. Print 2004. 4. siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference. Naito Y, Yamada T, Ui-Tei K, Morishita S, Saigo K.Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W124-9.

Applications

RNAi Knockdown

Gene Information

Entrez GeneID: 1891

Gene Name: ECH1

Gene Alias: HPXEL

Page 1 of 2 2016/5/20 Gene enoyl Coenzyme A hydratase 1, peroxisomal Description:

Omim ID: 600696

Gene Ontology: Hyperlink

Gene Summary: This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4- dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq

Other delta3,5-delta2,4-dienoyl-CoA isomerase,dienoyl-CoA Designations: isomerase,peroxisomal enoyl-CoA hydratase 1,peroxisomal enoyl- coenzyme A hydratase-like protein

Gene Pathway Tyrosine metabolism

Page 2 of 2 2016/5/20