Anti-ECH1 Antibody (ARG58672)

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Product datasheet [email protected] ARG58672 Package: 100 μl anti-ECH1 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes ECH1 Tested Reactivity Hu, Ms, Rat Tested Application IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name ECH1 Antigen Species Human Immunogen Recombinant fusion protein corresponding to aa. 1-328 of Human ECH1 (NP_001389.2). Conjugation Un-conjugated Alternate Names HPXEL; Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; EC 5.3.3.- Application Instructions Application table Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control BxPC3 Calculated Mw 36 kDa Observed Size 35 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol ECH1 Gene Full Name enoyl CoA hydratase 1, peroxisomal Background This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008] Function Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. [UniProt] Cellular Localization Mitochondrion, Peroxisome. [UniProt] Images ARG58672 anti-ECH1 antibody IHC-P image Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG58672 anti-ECH1 antibody at 1:150 dilution. ARG58672 anti-ECH1 antibody WB image Western blot: 25 µg of BxPC3 cell lysate stained with ARG58672 anti- ECH1 antibody at 1:3000 dilution. www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).

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Rabbit Anti-Human ECH1 Polyclonal Antibody (DPABH-07145) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use
Rabbit Anti-Human ECH1 Polyclonal Antibody (DPABH-07145) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Immunogen Recombinant Protein, antigen sequence: ISLRLTGSSAQEEASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVE CFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIE RCPK Isotype IgG Source/Host Rabbit Species Reactivity Human Purification Antigen affinity purified Conjugate Unconjugated Applications IHC, WB Format Liquid Size 100 μL Buffer 40% glycerol and PBS (pH 7.2). Preservative 0.02% Sodium Azide Storage Store at +4°C for short term storage. Long time storage is recommended at -20°C. Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. BACKGROUND Introduction This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5- cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl- CoA isomerase. This enzyme
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