Anti-ECH1 Antibody (ARG58672)

Product datasheet [email protected]

ARG58672 Package: 100 μl anti-ECH1 antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes ECH1

Tested Reactivity Hu, Ms, Rat

Tested Application IHC-P, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name ECH1

Antigen Species Human

Immunogen Recombinant fusion protein corresponding to aa. 1-328 of Human ECH1 (NP_001389.2).

Conjugation Un-conjugated

Alternate Names HPXEL; Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; EC 5.3.3.-

Application Instructions

Application table Application Dilution

IHC-P 1:50 - 1:200

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control BxPC3

Calculated Mw 36 kDa

Observed Size 35 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol ECH1

Gene Full Name enoyl CoA hydratase 1, peroxisomal

Background This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

Function Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. [UniProt]

Cellular Localization Mitochondrion, Peroxisome. [UniProt]

Images

ARG58672 anti-ECH1 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG58672 anti-ECH1 antibody at 1:150 dilution.

ARG58672 anti-ECH1 antibody WB image

Western blot: 25 µg of BxPC3 cell lysate stained with ARG58672 anti- ECH1 antibody at 1:3000 dilution.