Rabbit Anti-Human ECH1 Polyclonal Antibody (DPABH-07145) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

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Rabbit Anti-Human ECH1 Polyclonal Antibody (DPABH-07145) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Immunogen Recombinant Protein, antigen sequence: ISLRLTGSSAQEEASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVE CFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIE RCPK Isotype IgG Source/Host Rabbit Species Reactivity Human Purification Antigen affinity purified Conjugate Unconjugated Applications IHC, WB Format Liquid Size 100 μL Buffer 40% glycerol and PBS (pH 7.2). Preservative 0.02% Sodium Azide Storage Store at +4°C for short term storage. Long time storage is recommended at -20°C. Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. BACKGROUND Introduction This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5- cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl- CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008] Keywords ECH1; enoyl CoA hydratase 1, peroxisomal; HPXEL; delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; dienoyl-CoA isomerase; peroxisomal enoyl-CoA hydratase 1; delta3,5- delta2,4-dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; GENE INFORMATION Entrez Gene ID 1891 UniProt ID Q13011 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.

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PARSANA-DISSERTATION-2020.Pdf
DECIPHERING TRANSCRIPTIONAL PATTERNS OF GENE REGULATION: A COMPUTATIONAL APPROACH by Princy Parsana A dissertation submitted to The Johns Hopkins University in conformity with the requirements for the degree of Doctor of Philosophy Baltimore, Maryland July, 2020 © 2020 Princy Parsana All rights reserved Abstract With rapid advancements in sequencing technology, we now have the ability to sequence the entire human genome, and to quantify expression of tens of thousands of genes from hundreds of individuals. This provides an extraordinary opportunity to learn phenotype relevant genomic patterns that can improve our understanding of molecular and cellular processes underlying a trait. The high dimensional nature of genomic data presents a range of computational and statistical challenges. This dissertation presents a compilation of projects that were driven by the motivation to efficiently capture gene regulatory patterns in the human transcriptome, while addressing statistical and computational challenges that accompany this data. We attempt to address two major difficulties in this domain: a) artifacts and noise in transcriptomic data, andb) limited statistical power. First, we present our work on investigating the effect of artifactual variation in gene expression data and its impact on trans-eQTL discovery. Here we performed an in-depth analysis of diverse pre-recorded covariates and latent confounders to understand their contribution to heterogeneity in gene expression measurements. Next, we discovered 673 trans-eQTLs across 16 human tissues using v6 data from the Genotype Tissue Expression (GTEx) project. Finally, we characterized two trait-associated trans-eQTLs; one in Skeletal Muscle and another in Thyroid. Second, we present a principal component based residualization method to correct gene expression measurements prior to reconstruction of co-expression networks.
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Product datasheet [email protected] ARG58672 Package: 100 μl anti-ECH1 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes ECH1 Tested Reactivity Hu, Ms, Rat Tested Application IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name ECH1 Antigen Species Human Immunogen Recombinant fusion protein corresponding to aa. 1-328 of Human ECH1 (NP_001389.2). Conjugation Un-conjugated Alternate Names HPXEL; Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; EC 5.3.3.- Application Instructions Application table Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control BxPC3 Calculated Mw 36 kDa Observed Size 35 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol ECH1 Gene Full Name enoyl CoA hydratase 1, peroxisomal Background This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins.
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bioRxiv preprint doi: https://doi.org/10.1101/821637; this version posted November 1, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Identification of a fatty acid-sensitive interaction between mitochondrial uncoupling protein 3 and enoyl-CoA hydratase 1 in skeletal muscle Christine K. Dao1, Alexander Kenaston1, Katsuya Hirasaka2, Shohei Kohno2, Christopher Riley7, Gloria Fang1, Kristin Fathe3, Ashley Solmonson6,Sara M. Nowinski4, Matthew E. 1,2 5 2 1 Pfeiffer , Xianmei Yang , Takeshi Nikawa , and Edward M. Mills 1 Division of Pharmacology and Toxicology; University of Texas at Austin; Austin, TX; USA 2 Department of Nutritional Physiology; Institute of Health Biosciences; University of Tokushima; Tokushima; Japan 3Department of Chemistry and Biochemistry; University of Texas at Austin; Austin, TX; USA 4Department of Biochemistry; University of Utah School of Medicine; Salt Lake City, UT; USA 5 Institute of Biomedical Sciences; Fudan University; Shanghai, China 6Children's Medical Center Research Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. 7Department of Cancer Biology; Dana Farber Cancer Institute, Boston, MA; USA Address correspondence to: Edward M. Mills, PhD, University of Texas at Austin, College of Pharmacy Austin, TX 78714 USA Email: [email protected], Phone: (512) 471-6699, Fax: (512) 471-5002 1 bioRxiv preprint doi: https://doi.org/10.1101/821637; this version posted November 1, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.
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