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Florida International University FIU Digital Commons Robert Stempel College of Public Health & Social Environmental & Occupational Health Work 5-18-2017 European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS) Marcus Cooke Oxidative Stress Group, Department of Environmental and Occupational Health, Florida International University, [email protected] Follow this and additional works at: http://digitalcommons.fiu.edu/eoh Part of the Medicine and Health Sciences Commons Recommended Citation Cooke, Marcus, "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" (2017). Environmental & Occupational Health. 28. http://digitalcommons.fiu.edu/eoh/28 This work is brought to you for free and open access by the Robert Stempel College of Public Health & Social Work at FIU Digital Commons. It has been accepted for inclusion in Environmental & Occupational Health by an authorized administrator of FIU Digital Commons. For more information, please contact [email protected]. Redox Biology 13 (2017) 94–162 Contents lists available at ScienceDirect Redox Biology journal homepage: www.elsevier.com/locate/redox Review article European contribution to the study of ROS: A summary of the findings and MARK prospects for the future from the COST action BM1203 (EU-ROS) Javier Egeaa,1, Isabel Fabregatb,1, Yves M. Frapartc,1, Pietro Ghezzid,1, Agnes Görlache,f,1, Thomas Kietzmanng,1, Kateryna Kubaichukg,1,UllaG.Knaush,1, Manuela G. Lopeza,1, Gloria Olaso-Gonzalezi,1, Andreas Petrye,1, Rainer Schulzj,1, Jose Vinai,1,PaulWinyardk,1, Kahina Abbasc, Opeyemi S. Ademowol, Catarina B. Afonsom, Ioanna Andreadoun, Haike Antelmanno,FernandoAntunesp, Mutay Aslanq, Markus M. Bachschmidr, Rui M. Barbosas, Vsevolod Belousovt, Carsten Berndtu, David Bernlohrv,EstherBertránb, Alberto Bindoliw, Serge P. Bottarix, Paula M. Britoy,z,GuiaCarraraaa, Ana I. Casasab, Afroditi Chatziac, Niki Chondrogianniad, Marcus Conradae,MarcusS.Cookeaf,JoãoG.Costay,ag, Antonio Cuadradoah, Pham My-Chan Dangai, Barbara De Smetaj,aw,cp,cr, Bilge Debelec–Butunerak,IrundikaH.K.Diasl, Joe Dan Dunnal,AmandaJ.Edsonam, Mariam El Assaran, Jamel El-Bennaai, Péter Ferdinandyao,cr, Ana S. Fernandesag,KariE.Fladmarkam, Ulrich Förstermannap, Rashid Giniatullinaq, Zoltán Giriczao,cr,AnikóGörbeao,cr,HelenGriffithsl,ar, Vaclav Hamplas,AlinaHanfat, Jan Hergetas, Pablo Hernansanz-Agustínau,av, Melanie Hilliono, Jingjing Huangaj,aw,cq,cs,SerapIlikayax, Pidder Jansen-Dürray, Vincent Jaquetaz, Jaap A. Jolesba, Balaraman Kalyanaramanbb, Danylo Kaminskyybc, Mahsa Karbaschiaf,MarinaKleanthousbd, Lars-Oliver Klotzbe,BatoKoracbf, Kemal Sami Korkmazbg,RafalKozielay, Damir Kračune, Karl-Heinz Krauseaz,VladimírKřenbh, Thomas Kriegbi, João Laranjinhas,AntigoneLazoubj, Huige Liap, Antonio Martínez-Ruizau,bk, Reiko Matsuir, Gethin J. McBeanbl, Stuart P. Meredithm, Joris Messensaw,cs, Verónica Miguelbm, Yuliya Mikhedat, Irina Milisavbn, Lidija Milkovićbo, Antonio Miranda-Vizuetebp, Miloš Mojovićbq, María Monsalvebr, Pierre-Alexis Mouthuybs, John Mulveybi, Thomas Münzelat, Vladimir Muzykantovbt,IsabelT.N.Nguyenba, Matthias Oelzeat, Nuno G. Oliveiray, Carlos M. Palmeirabu,ct, Nikoletta Papaevgeniouad, Aleksandra Pavićevićbq, Brandán Pedreaw,cs, Fabienne Peyrotc,bv,MariosPhylactidesbd,GratielaG.Pircalabiorubw,AndrewR.Pittm, Henrik E. Poulsenbx,cu,cv, Ignacio Prietobr, Maria Pia Rigobelloby, Natalia Robledinos-Antónah, Leocadio Rodríguez-Mañasan,bz, Anabela P. Rolobu,ct, Francis Roussetaz, Tatjana Ruskovskacb, Nuno Saraivaag, Shlomo Sassoncc, Katrin Schrödercd,ce, Khrystyna Semenbc, Tamara Seredeninaaz, Anastasia Shakirzyanovaaq,Geoffrey L. Smithaa, Thierry Soldatial, Bebiana C. Sousam, Corinne M. Spickettl, Ana Stancicbf, Marie José Stasiacf,cg, Holger Steinbrennerbe,Višnja Stepanićbo, Sebastian Stevenat, Kostas Tokatlidisac, Erkan Tuncaych, Belma Turanch, Fulvio Ursinici, Jan Vacekcj, Olga Vajnerovaas,Kateřina Valentovábh,FrankVanBreusegemaj,cq,LokmanVarisliax, Elizabeth A. Vealck, A. Suha Yalçıncl, Olha Yelisyeyevabc, Neven Žarkovićbs, Martina Zatloukalovácj, Jacek Zielonkabb, Rhian M. Touyzcm,1, Andreas Papapetropouloscn,1,TilmanGruneco,1, ⁎ ⁎⁎ Santiago Lamasbm,1, Harald H.H.W. Schmidtab,1,FabioDiLisacp, ,1, Andreas Daiberat,ce, ,1 a Institute Teofilo Hernando, Department of Pharmacology, School of Medicine. Univerisdad Autonoma de Madrid, Spain b Bellvitge Biomedical Research Institute (IDIBELL) and University of Barcelona (UB), L’Hospitalet, Barcelona, Spain ⁎ Corresponding author at: Universitätsmedizin der Johannes Gutenberg-Universität Mainz, Zentrum für Kardiologie/Kardiologie 1, Langenbeckstr. 1, 55131 Mainz, Germany. ⁎⁎ Corresponding author at: Department of Biomedical Sciences, University of Padova, Italy. E-mail addresses: [email protected] (F. Di Lisa), [email protected] (A. Daiber). http://dx.doi.org/10.1016/j.redox.2017.05.007 Received 5 May 2017; Accepted 8 May 2017 Available online 18 May 2017 2213-2317/ © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). J. Egea et al. Redox Biology 13 (2017) 94–162 c LCBPT, UMR 8601 CNRS - Paris Descartes University, Sorbonne Paris Cité, Paris, France d Brighton & Sussex Medical School, Brighton, UK e Experimental and Molecular Pediatric Cardiology, German Heart Center Munich at the Technical University Munich, Munich, Germany f DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany g Faculty of Biochemistry and Molecular Medicine, and Biocenter Oulu, University of Oulu, Oulu, Finland h Conway Institute, School of Medicine, University College Dublin, Dublin, Ireland i Department of Physiology, University of Valencia, Spain j Institute of Physiology, JLU Giessen, Giessen, Germany k University of Exeter Medical School, St Luke's Campus, Exeter EX1 2LU, UK l Life & Health Sciences and Aston Research Centre for Healthy Ageing, Aston University, Aston Triangle, Birmingham B4 7ET, UK m School of Life & Health Sciences, Aston University, Aston Triangle, Birmingham B47ET, UK n Laboratory of Pharmacology, Faculty of Pharmacy, National and Kapodistrian University of Athens, Greece o Institute for Biology-Microbiology, Freie Universität Berlin, Berlin, Germany p Departamento de Química e Bioquímica and Centro de Química e Bioquímica, Faculdade de Ciências, Portugal q Department of Medical Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey r Vascular Biology Section & Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA, USA s Center for Neurosciences and Cell Biology, University of Coimbra and Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal t Molecular technologies laboratory, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, Moscow 117997, Russia u Department of Neurology, Medical Faculty, Heinrich-Heine University, Düsseldorf, Germany v Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota - Twin Cities, USA w Institute of Neuroscience (CNR), Padova, Italy x GETI, Institute for Advanced Biosciences, INSERM U1029, CNRS UMR 5309, Grenoble-Alpes University and Radio-analysis Laboratory, CHU de Grenoble, Grenoble, France y Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal z Faculdade de Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal aa Department of Pathology, University of Cambridge, Cambridge, UK ab Department of Pharmacology & Personalized Medicine, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands ac Institute of Molecular Cell and Systems Biology, College of Medical Veterinary and Life Sciences, University of Glasgow, University Avenue, Glasgow, UK ad National Hellenic Research Foundation, Institute of Biology, Medicinal Chemistry and Biotechnology, 48 Vas. Constantinou Ave., 116 35 Athens, Greece ae Helmholtz Center Munich, Institute of Developmental Genetics, Neuherberg, Germany af Oxidative Stress Group, Dept. Environmental & Occupational Health, Florida International University, Miami, FL 33199, USA ag CBIOS, Universidade Lusófona Research Center for Biosciences & Health Technologies, Lisboa, Portugal ah Instituto de Investigaciones Biomédicas “Alberto Sols” UAM-CSIC, Instituto de Investigación Sanitaria La Paz (IdiPaz), Department of Biochemistry, Faculty of Medicine, Autonomous University of Madrid. Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain ai Université Paris Diderot, Sorbonne Paris Cité, INSERM-U1149, CNRS-ERL8252, Centre de Recherche sur l’Inflammation, Laboratoire d’Excellence Inflamex, Faculté de Médecine Xavier Bichat, Paris, France aj Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium ak Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Ege University, Bornova, Izmir 35100, Turkey al Department of Biochemistry, Science II, University of Geneva, 30 quai Ernest-Ansermet, 1211 Geneva-4, Switzerland am Department of Molecular Biology, University of Bergen, Bergen, Norway an Fundación para la Investigación Biomédica del Hospital Universitario de Getafe, Getafe, Spain ao Department of Pharmacology and Pharmacotherapy, Medical Faculty, Semmelweis University, Budapest, Hungary ap Department of Pharmacology, Johannes Gutenberg
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  • Molecular Characterization, Protein–Protein Interaction Network, and Evolution of Four Glutathione Peroxidases from Tetrahymena Thermophila

    Molecular Characterization, Protein–Protein Interaction Network, and Evolution of Four Glutathione Peroxidases from Tetrahymena Thermophila

    antioxidants Article Molecular Characterization, Protein–Protein Interaction Network, and Evolution of Four Glutathione Peroxidases from Tetrahymena thermophila Diana Ferro 1,2, Rigers Bakiu 3 , Sandra Pucciarelli 4, Cristina Miceli 4 , Adriana Vallesi 4 , Paola Irato 5 and Gianfranco Santovito 5,* 1 BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA; [email protected] 2 Department of Pediatrics, Children’s Mercy Hospital and Clinics, Kansas City, MO 64108, USA 3 Department of Aquaculture and Fisheries, Agricultural University of Tirana, 1000 Tiranë, Albania; [email protected] 4 School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy; [email protected] (S.P.); [email protected] (C.M.); [email protected] (A.V.) 5 Department of Biology, University of Padova, 35131 Padova, Italy; [email protected] * Correspondence: [email protected] Received: 6 September 2020; Accepted: 1 October 2020; Published: 2 October 2020 Abstract: Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role of GPxs in reactive oxygen species detoxification in the unicellular eukaryotic model organism Tetrahymena thermophila. Both phylogenetic and mechanistic empirical model analyses provided indications about the evolutionary relationships among the GPXs of Tetrahymena and the orthologous enzymes of phylogenetically related species. In-silico gene characterization and text mining were used to predict the functional relationships between GPxs and other physiologically-relevant processes. The GPx genes contain conserved transcriptional regulatory elements in the promoter region, which suggest that transcription is under tight control of specialized signaling pathways.
  • Extracellular Glutathione Peroxidase Gpx3 and Its Role in Cancer

    Extracellular Glutathione Peroxidase Gpx3 and Its Role in Cancer

    cancers Review Extracellular Glutathione Peroxidase GPx3 and Its Role in Cancer Caroline Chang 1, Beth L. Worley 2,Rébécca Phaëton 3 and Nadine Hempel 2,* 1 Department of Comparative Medicine, Penn State University College of Medicine, Hershey, PA 17033, USA; [email protected] 2 Department of Pharmacology, Penn State University College of Medicine, Hershey, PA 17033, USA; [email protected] 3 Department of Obstetrics & Gynecology & Department of Microbiology and Immunology, Penn State University College of Medicine, Hershey, PA 17033, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-717-531-4037 Received: 29 June 2020; Accepted: 4 August 2020; Published: 6 August 2020 Abstract: Mammalian cells possess a multifaceted antioxidant enzyme system, which includes superoxide dismutases, catalase, the peroxiredoxin/thioredoxin and the glutathione peroxidase systems. The dichotomous role of reactive oxygen species and antioxidant enzymes in tumorigenesis and cancer progression complicates the use of small molecule antioxidants, pro-oxidants, and targeting of antioxidant enzymes as therapeutic approaches for cancer treatment. It also highlights the need for additional studies to investigate the role and regulation of these antioxidant enzymes in cancer. The focus of this review is on glutathione peroxidase 3 (GPx3), a selenoprotein, and the only extracellular GPx of a family of oxidoreductases that catalyze the detoxification of hydro- and soluble lipid hydroperoxides by reduced glutathione. In addition to summarizing the biochemical function, regulation, and disease associations of GPx3, we specifically discuss the role and regulation of systemic and tumor cell expressed GPx3 in cancer. From this it is evident that GPx3 has a dichotomous role in different tumor types, acting as both a tumor suppressor and pro-survival protein.